This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.06 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 441,032 (17.1%) Reads that were too short: 31,211 (1.2%) Reads written (passing filters): 409,821 (15.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 91,490,916 bp (11.8%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 441032 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 484 628.5 0 484 7 1749 628.5 0 1749 8 27060 628.5 0 27060 9 25046 628.5 0 25046 10 28059 628.5 0 28059 11 61 628.5 0 61 12 109 628.5 0 109 13 36 628.5 0 36 14 15 628.5 0 15 15 29 628.5 0 29 16 50 628.5 0 50 17 51 628.5 0 51 18 34 628.5 0 34 19 39 628.5 0 39 20 39 628.5 0 39 21 45 628.5 0 45 22 58 628.5 0 58 23 70 628.5 0 70 24 76 628.5 0 76 25 56 628.5 0 56 26 74 628.5 0 74 27 81 628.5 0 81 28 42 628.5 0 42 29 51 628.5 0 51 30 54 628.5 0 54 31 73 628.5 0 73 32 219 628.5 0 219 33 37 628.5 0 37 34 26 628.5 0 26 35 23 628.5 0 23 36 24 628.5 0 24 37 37 628.5 0 37 38 121 628.5 0 121 39 121 628.5 0 121 40 249 628.5 0 249 41 379 628.5 0 379 42 67 628.5 0 67 43 50 628.5 0 50 44 24 628.5 0 24 45 39 628.5 0 39 46 24 628.5 0 24 47 44 628.5 0 44 48 114 628.5 0 114 49 99 628.5 0 99 50 76 628.5 0 76 51 110 628.5 0 110 52 163 628.5 0 163 53 89 628.5 0 89 54 96 628.5 0 96 55 28 628.5 0 28 56 32 628.5 0 32 57 77 628.5 0 77 58 43 628.5 0 43 59 47 628.5 0 47 60 65 628.5 0 65 61 39 628.5 0 39 62 63 628.5 0 63 63 49 628.5 0 49 64 30 628.5 0 30 65 47 628.5 0 47 66 121 628.5 0 121 67 71 628.5 0 71 68 172 628.5 0 172 69 87 628.5 0 87 70 69 628.5 0 69 71 52 628.5 0 52 72 179 628.5 0 179 73 83 628.5 0 83 74 126 628.5 0 126 75 161 628.5 0 161 76 228 628.5 0 228 77 360 628.5 0 360 78 740 628.5 0 740 79 1417 628.5 0 1417 80 439 628.5 0 439 81 575 628.5 0 575 82 917 628.5 0 917 83 462 628.5 0 462 84 465 628.5 0 465 85 970 628.5 0 970 86 1251 628.5 0 1251 87 1342 628.5 0 1342 88 1277 628.5 0 1277 89 2093 628.5 0 2093 90 2964 628.5 0 2964 91 4070 628.5 0 4070 92 89779 628.5 0 89779 93 107812 628.5 0 107812 94 91298 628.5 0 91298 95 1759 628.5 0 1759 96 47 628.5 0 47 97 87 628.5 0 87 98 176 628.5 0 176 99 60 628.5 0 60 100 26 628.5 0 26 101 14 628.5 0 14 102 24 628.5 0 24 103 26 628.5 0 26 104 20 628.5 0 20 105 20 628.5 0 20 106 18 628.5 0 18 107 30 628.5 0 30 108 34 628.5 0 34 109 39 628.5 0 39 110 85 628.5 0 85 111 181 628.5 0 181 112 125 628.5 0 125 113 32 628.5 0 32 114 64 628.5 0 64 115 73 628.5 0 73 116 63 628.5 0 63 117 89 628.5 0 89 118 45 628.5 0 45 119 294 628.5 0 294 120 16 628.5 0 16 121 19 628.5 0 19 122 7 628.5 0 7 123 12 628.5 0 12 124 16 628.5 0 16 125 34 628.5 0 34 126 20 628.5 0 20 127 17 628.5 0 17 128 36 628.5 0 36 129 32 628.5 0 32 130 55 628.5 0 55 131 62 628.5 0 62 132 23 628.5 0 23 133 35 628.5 0 35 134 20 628.5 0 20 135 34 628.5 0 34 136 46 628.5 0 46 137 35 628.5 0 35 138 59 628.5 0 59 139 33 628.5 0 33 140 19 628.5 0 19 141 85 628.5 0 85 142 30 628.5 0 30 143 43 628.5 0 43 144 57 628.5 0 57 145 82 628.5 0 82 146 33 628.5 0 33 147 30 628.5 0 30 148 43 628.5 0 43 149 24 628.5 0 24 150 31 628.5 0 31 151 18 628.5 0 18 152 23 628.5 0 23 153 23 628.5 0 23 154 19 628.5 0 19 155 21 628.5 0 21 156 24 628.5 0 24 157 35 628.5 0 35 158 41 628.5 0 41 159 26 628.5 0 26 160 29 628.5 0 29 161 20 628.5 0 20 162 23 628.5 0 23 163 25 628.5 0 25 164 18 628.5 0 18 165 16 628.5 0 16 166 17 628.5 0 17 167 17 628.5 0 17 168 18 628.5 0 18 169 20 628.5 0 20 170 14 628.5 0 14 171 28 628.5 0 28 172 25 628.5 0 25 173 27 628.5 0 27 174 12 628.5 0 12 175 27 628.5 0 27 176 41 628.5 0 41 177 61 628.5 0 61 178 260 628.5 0 260 179 212 628.5 0 212 180 154 628.5 0 154 181 38 628.5 0 38 182 31 628.5 0 31 183 40 628.5 0 40 184 39 628.5 0 39 185 47 628.5 0 47 186 74 628.5 0 74 187 148 628.5 0 148 188 226 628.5 0 226 189 97 628.5 0 97 190 177 628.5 0 177 191 180 628.5 0 180 192 116 628.5 0 116 193 71 628.5 0 71 194 116 628.5 0 116 195 113 628.5 0 113 196 143 628.5 0 143 197 125 628.5 0 125 198 182 628.5 0 182 199 247 628.5 0 247 200 377 628.5 0 377 201 5549 628.5 0 5549 202 5452 628.5 0 5452 203 6962 628.5 0 6962 204 236 628.5 0 236 205 303 628.5 0 303 206 748 628.5 0 748 207 615 628.5 0 615 208 513 628.5 0 513 209 137 628.5 0 137 210 87 628.5 0 87 211 78 628.5 0 78 212 71 628.5 0 71 213 68 628.5 0 68 214 59 628.5 0 59 215 107 628.5 0 107 216 170 628.5 0 170 217 150 628.5 0 150 218 116 628.5 0 116 219 85 628.5 0 85 220 78 628.5 0 78 221 186 628.5 0 186 222 303 628.5 0 303 223 220 628.5 0 220 224 137 628.5 0 137 225 394 628.5 0 394 226 915 628.5 0 915 227 1077 628.5 0 1077 228 754 628.5 0 754 229 670 628.5 0 670 230 47 628.5 0 47 231 47 628.5 0 47 232 43 628.5 0 43 233 36 628.5 0 36 234 23 628.5 0 23 235 23 628.5 0 23 236 77 628.5 0 77 237 73 628.5 0 73 238 73 628.5 0 73 239 26 628.5 0 26 240 21 628.5 0 21 241 19 628.5 0 19 242 15 628.5 0 15 243 42 628.5 0 42 244 28 628.5 0 28 245 44 628.5 0 44 246 69 628.5 0 69 247 57 628.5 0 57 248 42 628.5 0 42 249 29 628.5 0 29 250 38 628.5 0 38 251 35 628.5 0 35 252 31 628.5 0 31 253 67 628.5 0 67 254 33 628.5 0 33 255 47 628.5 0 47 256 46 628.5 0 46 257 45 628.5 0 45 258 69 628.5 0 69 259 362 628.5 0 362 260 553 628.5 0 553 261 582 628.5 0 582 262 373 628.5 0 373 263 169 628.5 0 169 264 67 628.5 0 67 265 95 628.5 0 95 266 35 628.5 0 35 267 27 628.5 0 27 268 31 628.5 0 31 269 61 628.5 0 61 270 123 628.5 0 123 271 240 628.5 0 240 272 507 628.5 0 507 273 112 628.5 0 112 274 49 628.5 0 49 275 52 628.5 0 52 276 84 628.5 0 84 277 87 628.5 0 87 278 85 628.5 0 85 279 50 628.5 0 50 280 128 628.5 0 128 281 108 628.5 0 108 282 129 628.5 0 129 283 74 628.5 0 74 284 161 628.5 0 161 285 123 628.5 0 123 286 182 628.5 0 182 287 107 628.5 0 107 288 50 628.5 0 50 289 36 628.5 0 36 290 52 628.5 0 52 291 36 628.5 0 36 292 51 628.5 0 51 293 68 628.5 0 68 294 64 628.5 0 64 295 122 628.5 0 122 296 149 628.5 0 149 297 783 628.5 0 783 298 851 628.5 0 851 299 2189 628.5 0 2189 300 49 628.5 0 49 301 19 628.5 0 19 Finished in 17.52 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 409,821 Reads with adapters: 23,262 (5.7%) Reads that were too short: 215 (0.1%) Reads written (passing filters): 23,047 (5.6%) Total basepairs processed: 91,490,916 bp Total written (filtered): 6,219,974 bp (6.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23262 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 4 5 18 28 0.0 3 0 0 15 5 8 19 512 0.0 3 0 435 41 25 11 20 21838 0.0 4 19755 1586 342 115 40 21 49 0.0 4 11 32 4 0 2 22 13 0.0 4 5 1 2 1 4 23 129 0.0 4 1 0 0 84 44 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 1 27 1 0.0 4 1 31 1 0.0 4 1 38 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 42 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 67 5 0.0 4 0 0 0 0 5 68 54 0.0 4 0 0 0 0 54 76 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 1 115 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 5 0.0 4 0 0 0 1 4 123 4 0.0 4 0 0 0 1 3 124 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 4 0.0 4 0 0 0 0 4 127 372 0.0 4 0 0 0 0 372 128 4 0.0 4 0 0 0 0 4 129 3 0.0 4 0 0 0 0 3 130 4 0.0 4 0 0 0 0 4 136 11 0.0 4 0 0 0 0 11 137 2 0.0 4 0 0 0 1 1 138 3 0.0 4 0 0 0 0 3 140 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 148 8 0.0 4 0 0 0 1 7 149 32 0.0 4 0 0 0 2 30 150 13 0.0 4 0 0 0 0 13 151 68 0.0 4 0 0 0 3 65 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 169 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 172 4 0.0 4 0 0 0 0 4 173 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 184 2 0.0 4 0 0 0 0 2 185 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 1 194 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 0 0 0 2 198 4 0.0 4 0 0 0 0 4 199 1 0.0 4 0 0 0 1 200 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 3 0.0 4 0 0 0 0 3 211 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 233 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 258 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 285 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 23,047 Reads with adapters: 5,374 (23.3%) Reads written (passing filters): 23,047 (100.0%) Total basepairs processed: 6,219,974 bp Total written (filtered): 6,162,992 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5374 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 9.7% G: 85.0% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 360.1 0 16 4 3 90.0 0 3 7 2 1.4 0 2 8 7 0.4 0 7 9 989 0.1 0 120 869 10 3480 0.0 1 305 3175 11 821 0.0 1 40 781 12 16 0.0 1 1 15 13 17 0.0 1 0 17 14 1 0.0 1 0 1 150 11 0.0 1 11 152 3 0.0 1 3 169 2 0.0 1 2 208 5 0.0 1 2 3 209 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.