This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.71 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 415,071 (16.1%) Reads that were too short: 29,186 (1.1%) Reads written (passing filters): 385,885 (15.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 86,983,949 bp (11.2%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 415071 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 377 628.5 0 377 7 1696 628.5 0 1696 8 26034 628.5 0 26034 9 30513 628.5 0 30513 10 31261 628.5 0 31261 11 48 628.5 0 48 12 45 628.5 0 45 13 32 628.5 0 32 14 18 628.5 0 18 15 31 628.5 0 31 16 30 628.5 0 30 17 43 628.5 0 43 18 27 628.5 0 27 19 50 628.5 0 50 20 30 628.5 0 30 21 43 628.5 0 43 22 48 628.5 0 48 23 73 628.5 0 73 24 98 628.5 0 98 25 62 628.5 0 62 26 65 628.5 0 65 27 96 628.5 0 96 28 41 628.5 0 41 29 39 628.5 0 39 30 38 628.5 0 38 31 45 628.5 0 45 32 62 628.5 0 62 33 26 628.5 0 26 34 32 628.5 0 32 35 32 628.5 0 32 36 21 628.5 0 21 37 21 628.5 0 21 38 30 628.5 0 30 39 70 628.5 0 70 40 98 628.5 0 98 41 147 628.5 0 147 42 68 628.5 0 68 43 41 628.5 0 41 44 27 628.5 0 27 45 29 628.5 0 29 46 40 628.5 0 40 47 42 628.5 0 42 48 92 628.5 0 92 49 76 628.5 0 76 50 62 628.5 0 62 51 44 628.5 0 44 52 76 628.5 0 76 53 53 628.5 0 53 54 65 628.5 0 65 55 32 628.5 0 32 56 40 628.5 0 40 57 78 628.5 0 78 58 34 628.5 0 34 59 63 628.5 0 63 60 60 628.5 0 60 61 39 628.5 0 39 62 69 628.5 0 69 63 57 628.5 0 57 64 32 628.5 0 32 65 50 628.5 0 50 66 111 628.5 0 111 67 74 628.5 0 74 68 193 628.5 0 193 69 106 628.5 0 106 70 69 628.5 0 69 71 43 628.5 0 43 72 129 628.5 0 129 73 96 628.5 0 96 74 111 628.5 0 111 75 193 628.5 0 193 76 232 628.5 0 232 77 364 628.5 0 364 78 720 628.5 0 720 79 1426 628.5 0 1426 80 413 628.5 0 413 81 449 628.5 0 449 82 939 628.5 0 939 83 458 628.5 0 458 84 407 628.5 0 407 85 1046 628.5 0 1046 86 1269 628.5 0 1269 87 1238 628.5 0 1238 88 1019 628.5 0 1019 89 1612 628.5 0 1612 90 2213 628.5 0 2213 91 2716 628.5 0 2716 92 78134 628.5 0 78134 93 95134 628.5 0 95134 94 88257 628.5 0 88257 95 1729 628.5 0 1729 96 35 628.5 0 35 97 51 628.5 0 51 98 58 628.5 0 58 99 27 628.5 0 27 100 28 628.5 0 28 101 19 628.5 0 19 102 32 628.5 0 32 103 17 628.5 0 17 104 19 628.5 0 19 105 15 628.5 0 15 106 14 628.5 0 14 107 24 628.5 0 24 108 32 628.5 0 32 109 43 628.5 0 43 110 52 628.5 0 52 111 103 628.5 0 103 112 59 628.5 0 59 113 14 628.5 0 14 114 43 628.5 0 43 115 24 628.5 0 24 116 22 628.5 0 22 117 65 628.5 0 65 118 37 628.5 0 37 119 312 628.5 0 312 120 21 628.5 0 21 121 21 628.5 0 21 122 12 628.5 0 12 123 16 628.5 0 16 124 18 628.5 0 18 125 40 628.5 0 40 126 24 628.5 0 24 127 15 628.5 0 15 128 48 628.5 0 48 129 25 628.5 0 25 130 26 628.5 0 26 131 26 628.5 0 26 132 22 628.5 0 22 133 22 628.5 0 22 134 10 628.5 0 10 135 22 628.5 0 22 136 44 628.5 0 44 137 33 628.5 0 33 138 46 628.5 0 46 139 27 628.5 0 27 140 17 628.5 0 17 141 67 628.5 0 67 142 17 628.5 0 17 143 37 628.5 0 37 144 26 628.5 0 26 145 31 628.5 0 31 146 23 628.5 0 23 147 21 628.5 0 21 148 26 628.5 0 26 149 19 628.5 0 19 150 20 628.5 0 20 151 25 628.5 0 25 152 21 628.5 0 21 153 24 628.5 0 24 154 23 628.5 0 23 155 24 628.5 0 24 156 24 628.5 0 24 157 31 628.5 0 31 158 30 628.5 0 30 159 33 628.5 0 33 160 20 628.5 0 20 161 19 628.5 0 19 162 14 628.5 0 14 163 24 628.5 0 24 164 22 628.5 0 22 165 16 628.5 0 16 166 17 628.5 0 17 167 25 628.5 0 25 168 32 628.5 0 32 169 25 628.5 0 25 170 30 628.5 0 30 171 31 628.5 0 31 172 27 628.5 0 27 173 28 628.5 0 28 174 22 628.5 0 22 175 31 628.5 0 31 176 38 628.5 0 38 177 81 628.5 0 81 178 292 628.5 0 292 179 180 628.5 0 180 180 136 628.5 0 136 181 24 628.5 0 24 182 44 628.5 0 44 183 44 628.5 0 44 184 39 628.5 0 39 185 50 628.5 0 50 186 65 628.5 0 65 187 116 628.5 0 116 188 255 628.5 0 255 189 101 628.5 0 101 190 218 628.5 0 218 191 212 628.5 0 212 192 140 628.5 0 140 193 58 628.5 0 58 194 139 628.5 0 139 195 138 628.5 0 138 196 133 628.5 0 133 197 103 628.5 0 103 198 155 628.5 0 155 199 208 628.5 0 208 200 296 628.5 0 296 201 6444 628.5 0 6444 202 6116 628.5 0 6116 203 8649 628.5 0 8649 204 261 628.5 0 261 205 352 628.5 0 352 206 676 628.5 0 676 207 544 628.5 0 544 208 298 628.5 0 298 209 122 628.5 0 122 210 111 628.5 0 111 211 67 628.5 0 67 212 77 628.5 0 77 213 59 628.5 0 59 214 59 628.5 0 59 215 70 628.5 0 70 216 86 628.5 0 86 217 109 628.5 0 109 218 90 628.5 0 90 219 84 628.5 0 84 220 69 628.5 0 69 221 219 628.5 0 219 222 352 628.5 0 352 223 199 628.5 0 199 224 84 628.5 0 84 225 456 628.5 0 456 226 930 628.5 0 930 227 881 628.5 0 881 228 761 628.5 0 761 229 346 628.5 0 346 230 23 628.5 0 23 231 14 628.5 0 14 232 19 628.5 0 19 233 19 628.5 0 19 234 16 628.5 0 16 235 24 628.5 0 24 236 46 628.5 0 46 237 35 628.5 0 35 238 33 628.5 0 33 239 24 628.5 0 24 240 20 628.5 0 20 241 19 628.5 0 19 242 23 628.5 0 23 243 37 628.5 0 37 244 16 628.5 0 16 245 36 628.5 0 36 246 39 628.5 0 39 247 40 628.5 0 40 248 27 628.5 0 27 249 27 628.5 0 27 250 25 628.5 0 25 251 30 628.5 0 30 252 19 628.5 0 19 253 27 628.5 0 27 254 28 628.5 0 28 255 39 628.5 0 39 256 41 628.5 0 41 257 45 628.5 0 45 258 83 628.5 0 83 259 376 628.5 0 376 260 647 628.5 0 647 261 652 628.5 0 652 262 387 628.5 0 387 263 151 628.5 0 151 264 61 628.5 0 61 265 30 628.5 0 30 266 29 628.5 0 29 267 31 628.5 0 31 268 25 628.5 0 25 269 34 628.5 0 34 270 54 628.5 0 54 271 136 628.5 0 136 272 366 628.5 0 366 273 101 628.5 0 101 274 24 628.5 0 24 275 19 628.5 0 19 276 69 628.5 0 69 277 51 628.5 0 51 278 61 628.5 0 61 279 42 628.5 0 42 280 77 628.5 0 77 281 74 628.5 0 74 282 60 628.5 0 60 283 36 628.5 0 36 284 146 628.5 0 146 285 52 628.5 0 52 286 121 628.5 0 121 287 59 628.5 0 59 288 23 628.5 0 23 289 28 628.5 0 28 290 36 628.5 0 36 291 27 628.5 0 27 292 23 628.5 0 23 293 53 628.5 0 53 294 35 628.5 0 35 295 51 628.5 0 51 296 45 628.5 0 45 297 418 628.5 0 418 298 447 628.5 0 447 299 971 628.5 0 971 300 38 628.5 0 38 301 19 628.5 0 19 Finished in 14.62 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 385,885 Reads with adapters: 26,336 (6.8%) Reads that were too short: 148 (0.0%) Reads written (passing filters): 26,188 (6.8%) Total basepairs processed: 86,983,949 bp Total written (filtered): 6,903,077 bp (7.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26336 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 131 0.0 3 0 129 2 17 24001 0.0 3 23585 398 16 2 18 44 0.0 3 2 40 1 1 19 24 0.0 3 8 1 13 2 20 20 0.0 3 2 0 0 18 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 35 1 0.0 3 1 42 2 0.0 3 0 0 0 2 50 1 0.0 3 1 55 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 74 13 0.0 3 0 0 0 13 75 4 0.0 3 0 0 0 4 76 5 0.0 3 0 0 0 5 77 4 0.0 3 0 0 0 4 78 2 0.0 3 0 0 0 2 79 55 0.0 3 0 0 0 55 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 7 0.0 3 0 0 0 7 94 1 0.0 3 0 0 0 1 98 8 0.0 3 0 0 0 8 99 1172 0.0 3 0 0 2 1170 100 528 0.0 3 0 0 3 525 102 83 0.0 3 0 0 4 79 103 64 0.0 3 0 0 1 63 116 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 1 0.0 3 1 144 1 0.0 3 1 146 2 0.0 3 0 0 0 2 150 6 0.0 3 0 0 0 6 151 10 0.0 3 0 0 0 10 152 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 159 5 0.0 3 0 0 0 5 160 7 0.0 3 0 0 0 7 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 5 0.0 3 0 0 0 5 193 1 0.0 3 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 200 37 0.0 3 0 0 1 36 201 9 0.0 3 0 0 0 9 202 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 4 0.0 3 0 0 1 3 214 25 0.0 3 0 0 0 25 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 0 3 279 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 26,188 Reads with adapters: 109 (0.4%) Reads written (passing filters): 26,188 (100.0%) Total basepairs processed: 6,903,077 bp Total written (filtered): 6,902,709 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 109 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.1% C: 7.3% G: 14.7% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 93 409.2 0 93 4 10 102.3 0 10 5 2 25.6 0 2 6 1 6.4 0 1 11 3 0.0 1 0 3