This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.92 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 318,763 (12.4%) Reads that were too short: 9,430 (0.4%) Reads written (passing filters): 309,333 (12.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 68,540,254 bp (8.8%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 318763 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 72 628.5 0 72 7 6308 628.5 0 6308 8 3745 628.5 0 3745 9 19 628.5 0 19 10 28 628.5 0 28 11 65 628.5 0 65 12 13 628.5 0 13 13 5 628.5 0 5 14 4 628.5 0 4 15 6 628.5 0 6 16 5 628.5 0 5 17 10 628.5 0 10 18 9 628.5 0 9 19 11 628.5 0 11 20 17 628.5 0 17 21 44 628.5 0 44 22 22 628.5 0 22 23 14 628.5 0 14 24 7 628.5 0 7 25 11 628.5 0 11 26 16 628.5 0 16 27 28 628.5 0 28 28 77 628.5 0 77 29 17 628.5 0 17 30 21 628.5 0 21 31 11 628.5 0 11 32 10 628.5 0 10 33 19 628.5 0 19 34 20 628.5 0 20 35 41 628.5 0 41 36 40 628.5 0 40 37 6 628.5 0 6 38 10 628.5 0 10 39 25 628.5 0 25 40 24 628.5 0 24 41 22 628.5 0 22 42 72 628.5 0 72 43 41 628.5 0 41 44 45 628.5 0 45 45 55 628.5 0 55 46 55 628.5 0 55 47 37 628.5 0 37 48 17 628.5 0 17 49 76 628.5 0 76 50 26 628.5 0 26 51 56 628.5 0 56 52 459 628.5 0 459 53 109 628.5 0 109 54 67 628.5 0 67 55 93 628.5 0 93 56 18 628.5 0 18 57 21 628.5 0 21 58 16 628.5 0 16 59 132 628.5 0 132 60 98 628.5 0 98 61 222 628.5 0 222 62 604 628.5 0 604 63 112 628.5 0 112 64 284 628.5 0 284 65 281 628.5 0 281 66 467 628.5 0 467 67 839 628.5 0 839 68 284 628.5 0 284 69 271 628.5 0 271 70 105 628.5 0 105 71 160 628.5 0 160 72 483 628.5 0 483 73 516 628.5 0 516 74 963 628.5 0 963 75 1588 628.5 0 1588 76 1656 628.5 0 1656 77 4689 628.5 0 4689 78 6560 628.5 0 6560 79 119117 628.5 0 119117 80 38183 628.5 0 38183 81 107654 628.5 0 107654 82 645 628.5 0 645 83 226 628.5 0 226 84 43 628.5 0 43 85 18 628.5 0 18 86 19 628.5 0 19 87 22 628.5 0 22 88 14 628.5 0 14 89 14 628.5 0 14 90 11 628.5 0 11 91 9 628.5 0 9 92 13 628.5 0 13 93 11 628.5 0 11 94 12 628.5 0 12 95 15 628.5 0 15 96 13 628.5 0 13 97 7 628.5 0 7 98 9 628.5 0 9 99 10 628.5 0 10 100 14 628.5 0 14 101 9 628.5 0 9 102 19 628.5 0 19 103 19 628.5 0 19 104 12 628.5 0 12 105 13 628.5 0 13 106 12 628.5 0 12 107 14 628.5 0 14 108 13 628.5 0 13 109 19 628.5 0 19 110 12 628.5 0 12 111 18 628.5 0 18 112 15 628.5 0 15 113 7 628.5 0 7 114 21 628.5 0 21 115 14 628.5 0 14 116 114 628.5 0 114 117 128 628.5 0 128 118 351 628.5 0 351 119 16 628.5 0 16 120 27 628.5 0 27 121 36 628.5 0 36 122 38 628.5 0 38 123 77 628.5 0 77 124 50 628.5 0 50 125 39 628.5 0 39 126 31 628.5 0 31 127 27 628.5 0 27 128 21 628.5 0 21 129 41 628.5 0 41 130 96 628.5 0 96 131 185 628.5 0 185 132 230 628.5 0 230 133 32 628.5 0 32 134 15 628.5 0 15 135 39 628.5 0 39 136 42 628.5 0 42 137 76 628.5 0 76 138 18 628.5 0 18 139 39 628.5 0 39 140 77 628.5 0 77 141 116 628.5 0 116 142 199 628.5 0 199 143 48 628.5 0 48 144 56 628.5 0 56 145 61 628.5 0 61 146 73 628.5 0 73 147 102 628.5 0 102 148 82 628.5 0 82 149 115 628.5 0 115 150 1756 628.5 0 1756 151 2678 628.5 0 2678 152 2018 628.5 0 2018 153 36 628.5 0 36 154 20 628.5 0 20 155 28 628.5 0 28 156 18 628.5 0 18 157 19 628.5 0 19 158 53 628.5 0 53 159 11 628.5 0 11 160 19 628.5 0 19 161 17 628.5 0 17 162 17 628.5 0 17 163 17 628.5 0 17 164 27 628.5 0 27 165 26 628.5 0 26 166 29 628.5 0 29 167 49 628.5 0 49 168 42 628.5 0 42 169 74 628.5 0 74 170 57 628.5 0 57 171 33 628.5 0 33 172 107 628.5 0 107 173 77 628.5 0 77 174 74 628.5 0 74 175 20 628.5 0 20 176 9 628.5 0 9 177 8 628.5 0 8 178 13 628.5 0 13 179 20 628.5 0 20 180 25 628.5 0 25 181 19 628.5 0 19 182 19 628.5 0 19 183 24 628.5 0 24 184 19 628.5 0 19 185 26 628.5 0 26 186 37 628.5 0 37 187 33 628.5 0 33 188 45 628.5 0 45 189 19 628.5 0 19 190 19 628.5 0 19 191 21 628.5 0 21 192 22 628.5 0 22 193 30 628.5 0 30 194 19 628.5 0 19 195 22 628.5 0 22 196 30 628.5 0 30 197 35 628.5 0 35 198 37 628.5 0 37 199 34 628.5 0 34 200 32 628.5 0 32 201 22 628.5 0 22 202 16 628.5 0 16 203 21 628.5 0 21 204 20 628.5 0 20 205 39 628.5 0 39 206 22 628.5 0 22 207 14 628.5 0 14 208 36 628.5 0 36 209 41 628.5 0 41 210 43 628.5 0 43 211 34 628.5 0 34 212 44 628.5 0 44 213 45 628.5 0 45 214 38 628.5 0 38 215 48 628.5 0 48 216 54 628.5 0 54 217 406 628.5 0 406 218 254 628.5 0 254 219 655 628.5 0 655 220 46 628.5 0 46 221 40 628.5 0 40 222 94 628.5 0 94 223 49 628.5 0 49 224 118 628.5 0 118 225 245 628.5 0 245 226 2010 628.5 0 2010 227 493 628.5 0 493 228 1293 628.5 0 1293 229 46 628.5 0 46 230 34 628.5 0 34 231 22 628.5 0 22 232 35 628.5 0 35 233 23 628.5 0 23 234 31 628.5 0 31 235 24 628.5 0 24 236 19 628.5 0 19 237 18 628.5 0 18 238 29 628.5 0 29 239 30 628.5 0 30 240 36 628.5 0 36 241 33 628.5 0 33 242 30 628.5 0 30 243 30 628.5 0 30 244 30 628.5 0 30 245 34 628.5 0 34 246 45 628.5 0 45 247 41 628.5 0 41 248 52 628.5 0 52 249 28 628.5 0 28 250 36 628.5 0 36 251 40 628.5 0 40 252 28 628.5 0 28 253 45 628.5 0 45 254 25 628.5 0 25 255 29 628.5 0 29 256 39 628.5 0 39 257 39 628.5 0 39 258 34 628.5 0 34 259 22 628.5 0 22 260 30 628.5 0 30 261 23 628.5 0 23 262 38 628.5 0 38 263 21 628.5 0 21 264 32 628.5 0 32 265 31 628.5 0 31 266 55 628.5 0 55 267 30 628.5 0 30 268 34 628.5 0 34 269 33 628.5 0 33 270 28 628.5 0 28 271 25 628.5 0 25 272 34 628.5 0 34 273 53 628.5 0 53 274 110 628.5 0 110 275 153 628.5 0 153 276 52 628.5 0 52 277 180 628.5 0 180 278 37 628.5 0 37 279 59 628.5 0 59 280 53 628.5 0 53 281 40 628.5 0 40 282 31 628.5 0 31 283 25 628.5 0 25 284 57 628.5 0 57 285 39 628.5 0 39 286 88 628.5 0 88 287 46 628.5 0 46 288 40 628.5 0 40 289 51 628.5 0 51 290 60 628.5 0 60 291 68 628.5 0 68 292 81 628.5 0 81 293 44 628.5 0 44 294 35 628.5 0 35 295 29 628.5 0 29 296 36 628.5 0 36 297 40 628.5 0 40 298 68 628.5 0 68 299 58 628.5 0 58 300 88 628.5 0 88 301 70 628.5 0 70 Finished in 11.54 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 309,333 Reads with adapters: 3,480 (1.1%) Reads that were too short: 95 (0.0%) Reads written (passing filters): 3,385 (1.1%) Total basepairs processed: 68,540,254 bp Total written (filtered): 917,352 bp (1.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3480 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 24 0.0 3 0 24 17 3206 0.0 3 3142 64 18 6 0.0 3 0 6 19 11 0.0 3 7 0 4 20 6 0.0 3 1 0 0 5 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 26 5 0.0 3 2 0 0 3 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 41 63 0.0 3 0 0 0 63 43 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 45 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 1 74 1 0.0 3 0 0 0 1 76 7 0.0 3 0 0 0 7 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 8 0.0 3 0 0 0 8 109 7 0.0 3 0 0 0 7 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 1 124 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 0 4 134 5 0.0 3 0 0 0 5 135 1 0.0 3 0 0 0 1 142 4 0.0 3 0 0 0 4 144 3 0.0 3 0 0 0 3 146 3 0.0 3 0 0 0 3 149 6 0.0 3 0 0 0 6 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 188 1 0.0 3 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 207 9 0.0 3 0 0 0 9 208 5 0.0 3 0 0 0 5 209 15 0.0 3 0 0 0 15 210 13 0.0 3 0 0 0 13 211 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 222 4 0.0 3 0 0 0 4 225 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 3,385 Reads with adapters: 5 (0.1%) Reads written (passing filters): 3,385 (100.0%) Total basepairs processed: 917,352 bp Total written (filtered): 917,307 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 60.0% G: 40.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 52.9 0 2 13 3 0.0 1 0 3