This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.61 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 88,028 (3.4%) Reads that were too short: 6,924 (0.3%) Reads written (passing filters): 81,104 (3.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 21,148,386 bp (2.7%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 88028 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3433 628.5 0 3433 7 22237 628.5 0 22237 8 1143 628.5 0 1143 9 19770 628.5 0 19770 10 51 628.5 0 51 11 33 628.5 0 33 12 12 628.5 0 12 13 14 628.5 0 14 14 19 628.5 0 19 15 23 628.5 0 23 16 16 628.5 0 16 17 26 628.5 0 26 18 25 628.5 0 25 19 14 628.5 0 14 20 24 628.5 0 24 21 38 628.5 0 38 22 19 628.5 0 19 23 47 628.5 0 47 24 66 628.5 0 66 25 59 628.5 0 59 26 32 628.5 0 32 27 56 628.5 0 56 28 592 628.5 0 592 29 88 628.5 0 88 30 623 628.5 0 623 31 21 628.5 0 21 32 19 628.5 0 19 33 90 628.5 0 90 34 333 628.5 0 333 35 386 628.5 0 386 36 36 628.5 0 36 37 51 628.5 0 51 38 27 628.5 0 27 39 48 628.5 0 48 40 20 628.5 0 20 41 34 628.5 0 34 42 38 628.5 0 38 43 34 628.5 0 34 44 40 628.5 0 40 45 19 628.5 0 19 46 33 628.5 0 33 47 36 628.5 0 36 48 48 628.5 0 48 49 101 628.5 0 101 50 149 628.5 0 149 51 156 628.5 0 156 52 283 628.5 0 283 53 455 628.5 0 455 54 5049 628.5 0 5049 55 3409 628.5 0 3409 56 8610 628.5 0 8610 57 60 628.5 0 60 58 34 628.5 0 34 59 28 628.5 0 28 60 47 628.5 0 47 61 16 628.5 0 16 62 17 628.5 0 17 63 19 628.5 0 19 64 18 628.5 0 18 65 19 628.5 0 19 66 22 628.5 0 22 67 52 628.5 0 52 68 45 628.5 0 45 69 28 628.5 0 28 70 27 628.5 0 27 71 10 628.5 0 10 72 16 628.5 0 16 73 22 628.5 0 22 74 20 628.5 0 20 75 19 628.5 0 19 76 13 628.5 0 13 77 18 628.5 0 18 78 20 628.5 0 20 79 23 628.5 0 23 80 18 628.5 0 18 81 55 628.5 0 55 82 24 628.5 0 24 83 25 628.5 0 25 84 29 628.5 0 29 85 22 628.5 0 22 86 28 628.5 0 28 87 21 628.5 0 21 88 28 628.5 0 28 89 36 628.5 0 36 90 32 628.5 0 32 91 36 628.5 0 36 92 38 628.5 0 38 93 61 628.5 0 61 94 733 628.5 0 733 95 1027 628.5 0 1027 96 405 628.5 0 405 97 22 628.5 0 22 98 10 628.5 0 10 99 17 628.5 0 17 100 9 628.5 0 9 101 22 628.5 0 22 102 20 628.5 0 20 103 21 628.5 0 21 104 17 628.5 0 17 105 21 628.5 0 21 106 10 628.5 0 10 107 18 628.5 0 18 108 36 628.5 0 36 109 254 628.5 0 254 110 144 628.5 0 144 111 369 628.5 0 369 112 25 628.5 0 25 113 23 628.5 0 23 114 11 628.5 0 11 115 21 628.5 0 21 116 22 628.5 0 22 117 26 628.5 0 26 118 51 628.5 0 51 119 124 628.5 0 124 120 88 628.5 0 88 121 642 628.5 0 642 122 283 628.5 0 283 123 225 628.5 0 225 124 26 628.5 0 26 125 26 628.5 0 26 126 26 628.5 0 26 127 19 628.5 0 19 128 42 628.5 0 42 129 26 628.5 0 26 130 27 628.5 0 27 131 34 628.5 0 34 132 43 628.5 0 43 133 23 628.5 0 23 134 41 628.5 0 41 135 34 628.5 0 34 136 118 628.5 0 118 137 223 628.5 0 223 138 99 628.5 0 99 139 93 628.5 0 93 140 98 628.5 0 98 141 172 628.5 0 172 142 44 628.5 0 44 143 27 628.5 0 27 144 34 628.5 0 34 145 49 628.5 0 49 146 45 628.5 0 45 147 73 628.5 0 73 148 96 628.5 0 96 149 96 628.5 0 96 150 86 628.5 0 86 151 28 628.5 0 28 152 37 628.5 0 37 153 37 628.5 0 37 154 27 628.5 0 27 155 37 628.5 0 37 156 38 628.5 0 38 157 38 628.5 0 38 158 46 628.5 0 46 159 50 628.5 0 50 160 128 628.5 0 128 161 320 628.5 0 320 162 400 628.5 0 400 163 120 628.5 0 120 164 31 628.5 0 31 165 91 628.5 0 91 166 151 628.5 0 151 167 301 628.5 0 301 168 280 628.5 0 280 169 34 628.5 0 34 170 28 628.5 0 28 171 32 628.5 0 32 172 30 628.5 0 30 173 51 628.5 0 51 174 61 628.5 0 61 175 194 628.5 0 194 176 198 628.5 0 198 177 186 628.5 0 186 178 225 628.5 0 225 179 121 628.5 0 121 180 972 628.5 0 972 181 290 628.5 0 290 182 474 628.5 0 474 183 41 628.5 0 41 184 29 628.5 0 29 185 29 628.5 0 29 186 52 628.5 0 52 187 27 628.5 0 27 188 50 628.5 0 50 189 21 628.5 0 21 190 18 628.5 0 18 191 29 628.5 0 29 192 26 628.5 0 26 193 45 628.5 0 45 194 48 628.5 0 48 195 32 628.5 0 32 196 20 628.5 0 20 197 22 628.5 0 22 198 36 628.5 0 36 199 33 628.5 0 33 200 22 628.5 0 22 201 21 628.5 0 21 202 29 628.5 0 29 203 59 628.5 0 59 204 66 628.5 0 66 205 60 628.5 0 60 206 36 628.5 0 36 207 42 628.5 0 42 208 47 628.5 0 47 209 58 628.5 0 58 210 69 628.5 0 69 211 49 628.5 0 49 212 20 628.5 0 20 213 51 628.5 0 51 214 42 628.5 0 42 215 32 628.5 0 32 216 25 628.5 0 25 217 36 628.5 0 36 218 39 628.5 0 39 219 58 628.5 0 58 220 45 628.5 0 45 221 42 628.5 0 42 222 46 628.5 0 46 223 72 628.5 0 72 224 76 628.5 0 76 225 84 628.5 0 84 226 104 628.5 0 104 227 43 628.5 0 43 228 53 628.5 0 53 229 84 628.5 0 84 230 102 628.5 0 102 231 77 628.5 0 77 232 42 628.5 0 42 233 33 628.5 0 33 234 16 628.5 0 16 235 23 628.5 0 23 236 29 628.5 0 29 237 28 628.5 0 28 238 59 628.5 0 59 239 38 628.5 0 38 240 46 628.5 0 46 241 27 628.5 0 27 242 39 628.5 0 39 243 26 628.5 0 26 244 39 628.5 0 39 245 31 628.5 0 31 246 49 628.5 0 49 247 52 628.5 0 52 248 31 628.5 0 31 249 46 628.5 0 46 250 33 628.5 0 33 251 29 628.5 0 29 252 55 628.5 0 55 253 52 628.5 0 52 254 91 628.5 0 91 255 122 628.5 0 122 256 133 628.5 0 133 257 130 628.5 0 130 258 76 628.5 0 76 259 55 628.5 0 55 260 53 628.5 0 53 261 52 628.5 0 52 262 259 628.5 0 259 263 181 628.5 0 181 264 328 628.5 0 328 265 41 628.5 0 41 266 49 628.5 0 49 267 48 628.5 0 48 268 49 628.5 0 49 269 40 628.5 0 40 270 33 628.5 0 33 271 48 628.5 0 48 272 66 628.5 0 66 273 82 628.5 0 82 274 52 628.5 0 52 275 74 628.5 0 74 276 49 628.5 0 49 277 49 628.5 0 49 278 128 628.5 0 128 279 161 628.5 0 161 280 239 628.5 0 239 281 89 628.5 0 89 282 121 628.5 0 121 283 126 628.5 0 126 284 146 628.5 0 146 285 93 628.5 0 93 286 33 628.5 0 33 287 57 628.5 0 57 288 55 628.5 0 55 289 57 628.5 0 57 290 65 628.5 0 65 291 102 628.5 0 102 292 117 628.5 0 117 293 106 628.5 0 106 294 66 628.5 0 66 295 52 628.5 0 52 296 46 628.5 0 46 297 50 628.5 0 50 298 47 628.5 0 47 299 70 628.5 0 70 300 130 628.5 0 130 301 139 628.5 0 139 Finished in 4.21 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 81,104 Reads with adapters: 21,171 (26.1%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 21,149 (26.1%) Total basepairs processed: 21,148,386 bp Total written (filtered): 5,710,169 bp (27.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21171 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 17 0.0 3 0 0 14 1 2 19 294 0.0 3 0 228 38 16 12 20 19883 0.0 4 18404 1167 230 66 16 21 65 0.0 4 11 46 4 4 22 22 0.0 4 10 3 4 4 1 23 62 0.0 4 1 1 1 40 19 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 0 0 1 0 1 27 1 0.0 4 0 1 53 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 75 3 0.0 4 0 0 0 0 3 76 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 13 0.0 4 0 0 0 0 13 127 721 0.0 4 0 0 0 0 721 128 3 0.0 4 0 0 0 0 3 129 48 0.0 4 0 0 0 1 47 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 1 169 2 0.0 4 0 0 0 0 2 185 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.73 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 21,149 Reads with adapters: 999 (4.7%) Reads written (passing filters): 21,149 (100.0%) Total basepairs processed: 5,710,169 bp Total written (filtered): 5,698,778 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 999 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.8% C: 13.6% G: 72.5% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 117 330.5 0 117 4 28 82.6 0 28 5 1 20.7 0 1 9 8 0.1 0 2 6 10 25 0.0 1 1 24 11 290 0.0 1 11 279 12 500 0.0 1 14 486 13 20 0.0 1 1 19 15 3 0.0 1 0 3 18 1 0.0 1 1 152 2 0.0 1 1 1 153 1 0.0 1 1 210 2 0.0 1 1 1 211 1 0.0 1 1