This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.43 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 207,361 (8.1%) Reads that were too short: 14,277 (0.6%) Reads written (passing filters): 193,084 (7.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 52,629,963 bp (6.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 207361 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1167 628.5 0 1167 7 2398 628.5 0 2398 8 81548 628.5 0 81548 9 68597 628.5 0 68597 10 121 628.5 0 121 11 83 628.5 0 83 12 55 628.5 0 55 13 55 628.5 0 55 14 65 628.5 0 65 15 50 628.5 0 50 16 60 628.5 0 60 17 54 628.5 0 54 18 49 628.5 0 49 19 40 628.5 0 40 20 56 628.5 0 56 21 43 628.5 0 43 22 53 628.5 0 53 23 73 628.5 0 73 24 50 628.5 0 50 25 52 628.5 0 52 26 57 628.5 0 57 27 65 628.5 0 65 28 65 628.5 0 65 29 56 628.5 0 56 30 48 628.5 0 48 31 90 628.5 0 90 32 141 628.5 0 141 33 54 628.5 0 54 34 50 628.5 0 50 35 59 628.5 0 59 36 58 628.5 0 58 37 55 628.5 0 55 38 73 628.5 0 73 39 50 628.5 0 50 40 56 628.5 0 56 41 106 628.5 0 106 42 65 628.5 0 65 43 71 628.5 0 71 44 64 628.5 0 64 45 183 628.5 0 183 46 91 628.5 0 91 47 144 628.5 0 144 48 47 628.5 0 47 49 68 628.5 0 68 50 46 628.5 0 46 51 50 628.5 0 50 52 43 628.5 0 43 53 53 628.5 0 53 54 49 628.5 0 49 55 50 628.5 0 50 56 76 628.5 0 76 57 50 628.5 0 50 58 69 628.5 0 69 59 75 628.5 0 75 60 70 628.5 0 70 61 63 628.5 0 63 62 103 628.5 0 103 63 70 628.5 0 70 64 55 628.5 0 55 65 119 628.5 0 119 66 183 628.5 0 183 67 87 628.5 0 87 68 103 628.5 0 103 69 129 628.5 0 129 70 118 628.5 0 118 71 149 628.5 0 149 72 308 628.5 0 308 73 218 628.5 0 218 74 153 628.5 0 153 75 182 628.5 0 182 76 203 628.5 0 203 77 219 628.5 0 219 78 284 628.5 0 284 79 5186 628.5 0 5186 80 6318 628.5 0 6318 81 4187 628.5 0 4187 82 152 628.5 0 152 83 204 628.5 0 204 84 179 628.5 0 179 85 62 628.5 0 62 86 46 628.5 0 46 87 42 628.5 0 42 88 59 628.5 0 59 89 55 628.5 0 55 90 51 628.5 0 51 91 61 628.5 0 61 92 77 628.5 0 77 93 68 628.5 0 68 94 54 628.5 0 54 95 75 628.5 0 75 96 65 628.5 0 65 97 80 628.5 0 80 98 137 628.5 0 137 99 155 628.5 0 155 100 101 628.5 0 101 101 69 628.5 0 69 102 76 628.5 0 76 103 79 628.5 0 79 104 97 628.5 0 97 105 83 628.5 0 83 106 68 628.5 0 68 107 84 628.5 0 84 108 102 628.5 0 102 109 78 628.5 0 78 110 75 628.5 0 75 111 92 628.5 0 92 112 99 628.5 0 99 113 71 628.5 0 71 114 71 628.5 0 71 115 83 628.5 0 83 116 78 628.5 0 78 117 102 628.5 0 102 118 80 628.5 0 80 119 86 628.5 0 86 120 99 628.5 0 99 121 132 628.5 0 132 122 109 628.5 0 109 123 123 628.5 0 123 124 164 628.5 0 164 125 324 628.5 0 324 126 311 628.5 0 311 127 304 628.5 0 304 128 184 628.5 0 184 129 219 628.5 0 219 130 259 628.5 0 259 131 115 628.5 0 115 132 81 628.5 0 81 133 72 628.5 0 72 134 112 628.5 0 112 135 109 628.5 0 109 136 121 628.5 0 121 137 167 628.5 0 167 138 133 628.5 0 133 139 290 628.5 0 290 140 188 628.5 0 188 141 619 628.5 0 619 142 204 628.5 0 204 143 236 628.5 0 236 144 105 628.5 0 105 145 82 628.5 0 82 146 86 628.5 0 86 147 123 628.5 0 123 148 80 628.5 0 80 149 63 628.5 0 63 150 120 628.5 0 120 151 129 628.5 0 129 152 161 628.5 0 161 153 81 628.5 0 81 154 72 628.5 0 72 155 81 628.5 0 81 156 78 628.5 0 78 157 81 628.5 0 81 158 165 628.5 0 165 159 220 628.5 0 220 160 201 628.5 0 201 161 90 628.5 0 90 162 75 628.5 0 75 163 118 628.5 0 118 164 231 628.5 0 231 165 220 628.5 0 220 166 155 628.5 0 155 167 77 628.5 0 77 168 77 628.5 0 77 169 77 628.5 0 77 170 96 628.5 0 96 171 90 628.5 0 90 172 63 628.5 0 63 173 67 628.5 0 67 174 69 628.5 0 69 175 57 628.5 0 57 176 57 628.5 0 57 177 118 628.5 0 118 178 109 628.5 0 109 179 132 628.5 0 132 180 143 628.5 0 143 181 176 628.5 0 176 182 1883 628.5 0 1883 183 1010 628.5 0 1010 184 1290 628.5 0 1290 185 77 628.5 0 77 186 58 628.5 0 58 187 52 628.5 0 52 188 63 628.5 0 63 189 72 628.5 0 72 190 64 628.5 0 64 191 81 628.5 0 81 192 61 628.5 0 61 193 76 628.5 0 76 194 73 628.5 0 73 195 76 628.5 0 76 196 60 628.5 0 60 197 58 628.5 0 58 198 71 628.5 0 71 199 56 628.5 0 56 200 73 628.5 0 73 201 81 628.5 0 81 202 72 628.5 0 72 203 83 628.5 0 83 204 86 628.5 0 86 205 99 628.5 0 99 206 157 628.5 0 157 207 219 628.5 0 219 208 138 628.5 0 138 209 134 628.5 0 134 210 94 628.5 0 94 211 116 628.5 0 116 212 150 628.5 0 150 213 175 628.5 0 175 214 108 628.5 0 108 215 98 628.5 0 98 216 87 628.5 0 87 217 79 628.5 0 79 218 116 628.5 0 116 219 89 628.5 0 89 220 125 628.5 0 125 221 119 628.5 0 119 222 97 628.5 0 97 223 131 628.5 0 131 224 147 628.5 0 147 225 146 628.5 0 146 226 162 628.5 0 162 227 153 628.5 0 153 228 140 628.5 0 140 229 68 628.5 0 68 230 86 628.5 0 86 231 135 628.5 0 135 232 145 628.5 0 145 233 151 628.5 0 151 234 90 628.5 0 90 235 90 628.5 0 90 236 103 628.5 0 103 237 106 628.5 0 106 238 104 628.5 0 104 239 123 628.5 0 123 240 85 628.5 0 85 241 110 628.5 0 110 242 95 628.5 0 95 243 70 628.5 0 70 244 64 628.5 0 64 245 69 628.5 0 69 246 77 628.5 0 77 247 94 628.5 0 94 248 85 628.5 0 85 249 87 628.5 0 87 250 100 628.5 0 100 251 104 628.5 0 104 252 98 628.5 0 98 253 239 628.5 0 239 254 502 628.5 0 502 255 732 628.5 0 732 256 218 628.5 0 218 257 110 628.5 0 110 258 73 628.5 0 73 259 103 628.5 0 103 260 220 628.5 0 220 261 291 628.5 0 291 262 171 628.5 0 171 263 161 628.5 0 161 264 375 628.5 0 375 265 408 628.5 0 408 266 1532 628.5 0 1532 267 87 628.5 0 87 268 81 628.5 0 81 269 93 628.5 0 93 270 122 628.5 0 122 271 92 628.5 0 92 272 89 628.5 0 89 273 108 628.5 0 108 274 98 628.5 0 98 275 130 628.5 0 130 276 158 628.5 0 158 277 149 628.5 0 149 278 119 628.5 0 119 279 89 628.5 0 89 280 85 628.5 0 85 281 84 628.5 0 84 282 110 628.5 0 110 283 76 628.5 0 76 284 87 628.5 0 87 285 73 628.5 0 73 286 83 628.5 0 83 287 83 628.5 0 83 288 96 628.5 0 96 289 167 628.5 0 167 290 114 628.5 0 114 291 94 628.5 0 94 292 96 628.5 0 96 293 89 628.5 0 89 294 78 628.5 0 78 295 85 628.5 0 85 296 86 628.5 0 86 297 85 628.5 0 85 298 98 628.5 0 98 299 81 628.5 0 81 300 98 628.5 0 98 301 120 628.5 0 120 Finished in 9.35 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 193,084 Reads with adapters: 74,151 (38.4%) Reads that were too short: 90 (0.0%) Reads written (passing filters): 74,061 (38.4%) Total basepairs processed: 52,629,963 bp Total written (filtered): 20,009,990 bp (38.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 74151 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 14 0.0 3 0 0 0 5 9 18 59 0.0 3 0 0 29 18 12 19 1034 0.0 3 0 817 95 88 34 20 71029 0.0 4 64928 4656 1003 318 124 21 159 0.0 4 34 105 12 5 3 22 20 0.0 4 0 0 10 4 6 23 422 0.0 4 2 1 0 260 159 24 4 0.0 4 2 0 0 1 1 27 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 1 55 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 3 0.0 4 0 0 0 0 3 73 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 78 2 0.0 4 0 0 0 1 1 83 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 124 1 0.0 4 1 125 6 0.0 4 0 0 0 0 6 126 18 0.0 4 0 0 0 0 18 127 1253 0.0 4 0 0 0 1 1252 128 6 0.0 4 0 0 0 0 6 130 12 0.0 4 0 0 0 0 12 131 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 141 2 0.0 4 1 0 0 0 1 146 3 0.0 4 0 0 0 0 3 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 161 3 0.0 4 0 0 0 0 3 162 9 0.0 4 0 0 0 0 9 164 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 0 2 208 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 210 4 0.0 4 0 0 0 0 4 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 225 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 1 6 229 7 0.0 4 0 0 0 1 6 230 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 271 2 0.0 4 0 0 0 0 2 281 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 0 1 283 3 0.0 4 0 0 0 0 3 284 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 293 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.73 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 74,061 Reads with adapters: 17,229 (23.3%) Reads written (passing filters): 74,061 (100.0%) Total basepairs processed: 20,009,990 bp Total written (filtered): 19,791,015 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 17229 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 9.5% G: 85.1% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 1157.2 0 27 4 16 289.3 0 16 5 1 72.3 0 1 6 4 18.1 0 4 7 2 4.5 0 2 8 2 1.1 0 2 9 2167 0.3 0 289 1878 10 12921 0.1 1 1050 11871 11 1773 0.0 1 70 1703 12 45 0.0 1 0 45 13 44 0.0 1 0 44 14 2 0.0 1 0 2 39 1 0.0 1 1 147 1 0.0 1 1 150 8 0.0 1 6 2 151 1 0.0 1 1 168 1 0.0 1 0 1 169 3 0.0 1 2 1 177 1 0.0 1 0 1 189 1 0.0 1 0 1 208 9 0.0 1 7 2 220 1 0.0 1 1 223 172 0.0 1 150 22 224 6 0.0 1 5 1 225 3 0.0 1 2 1 226 1 0.0 1 1 257 1 0.0 1 1 259 13 0.0 1 12 1 260 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.