This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.47 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 223,362 (8.7%) Reads that were too short: 15,388 (0.6%) Reads written (passing filters): 207,974 (8.1%) Total basepairs processed: 774,927,510 bp Total written (filtered): 56,514,296 bp (7.3%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 223362 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 849 628.5 0 849 7 1625 628.5 0 1625 8 78518 628.5 0 78518 9 81604 628.5 0 81604 10 147 628.5 0 147 11 107 628.5 0 107 12 73 628.5 0 73 13 98 628.5 0 98 14 113 628.5 0 113 15 56 628.5 0 56 16 69 628.5 0 69 17 70 628.5 0 70 18 77 628.5 0 77 19 63 628.5 0 63 20 66 628.5 0 66 21 77 628.5 0 77 22 92 628.5 0 92 23 80 628.5 0 80 24 89 628.5 0 89 25 94 628.5 0 94 26 95 628.5 0 95 27 95 628.5 0 95 28 98 628.5 0 98 29 108 628.5 0 108 30 80 628.5 0 80 31 95 628.5 0 95 32 127 628.5 0 127 33 69 628.5 0 69 34 82 628.5 0 82 35 109 628.5 0 109 36 79 628.5 0 79 37 79 628.5 0 79 38 101 628.5 0 101 39 84 628.5 0 84 40 88 628.5 0 88 41 124 628.5 0 124 42 70 628.5 0 70 43 82 628.5 0 82 44 101 628.5 0 101 45 197 628.5 0 197 46 117 628.5 0 117 47 149 628.5 0 149 48 93 628.5 0 93 49 117 628.5 0 117 50 103 628.5 0 103 51 76 628.5 0 76 52 84 628.5 0 84 53 91 628.5 0 91 54 82 628.5 0 82 55 89 628.5 0 89 56 107 628.5 0 107 57 123 628.5 0 123 58 102 628.5 0 102 59 98 628.5 0 98 60 94 628.5 0 94 61 88 628.5 0 88 62 112 628.5 0 112 63 106 628.5 0 106 64 128 628.5 0 128 65 169 628.5 0 169 66 218 628.5 0 218 67 110 628.5 0 110 68 139 628.5 0 139 69 158 628.5 0 158 70 136 628.5 0 136 71 182 628.5 0 182 72 204 628.5 0 204 73 262 628.5 0 262 74 159 628.5 0 159 75 175 628.5 0 175 76 249 628.5 0 249 77 265 628.5 0 265 78 289 628.5 0 289 79 5559 628.5 0 5559 80 7048 628.5 0 7048 81 4770 628.5 0 4770 82 187 628.5 0 187 83 190 628.5 0 190 84 176 628.5 0 176 85 109 628.5 0 109 86 113 628.5 0 113 87 109 628.5 0 109 88 137 628.5 0 137 89 119 628.5 0 119 90 113 628.5 0 113 91 109 628.5 0 109 92 125 628.5 0 125 93 110 628.5 0 110 94 119 628.5 0 119 95 126 628.5 0 126 96 125 628.5 0 125 97 161 628.5 0 161 98 123 628.5 0 123 99 130 628.5 0 130 100 136 628.5 0 136 101 123 628.5 0 123 102 117 628.5 0 117 103 118 628.5 0 118 104 124 628.5 0 124 105 134 628.5 0 134 106 118 628.5 0 118 107 155 628.5 0 155 108 173 628.5 0 173 109 137 628.5 0 137 110 96 628.5 0 96 111 129 628.5 0 129 112 141 628.5 0 141 113 119 628.5 0 119 114 104 628.5 0 104 115 118 628.5 0 118 116 132 628.5 0 132 117 163 628.5 0 163 118 100 628.5 0 100 119 124 628.5 0 124 120 127 628.5 0 127 121 123 628.5 0 123 122 156 628.5 0 156 123 130 628.5 0 130 124 196 628.5 0 196 125 210 628.5 0 210 126 235 628.5 0 235 127 213 628.5 0 213 128 198 628.5 0 198 129 228 628.5 0 228 130 241 628.5 0 241 131 143 628.5 0 143 132 119 628.5 0 119 133 103 628.5 0 103 134 126 628.5 0 126 135 108 628.5 0 108 136 143 628.5 0 143 137 155 628.5 0 155 138 172 628.5 0 172 139 233 628.5 0 233 140 188 628.5 0 188 141 614 628.5 0 614 142 240 628.5 0 240 143 248 628.5 0 248 144 150 628.5 0 150 145 102 628.5 0 102 146 128 628.5 0 128 147 187 628.5 0 187 148 102 628.5 0 102 149 104 628.5 0 104 150 123 628.5 0 123 151 157 628.5 0 157 152 161 628.5 0 161 153 114 628.5 0 114 154 110 628.5 0 110 155 125 628.5 0 125 156 109 628.5 0 109 157 122 628.5 0 122 158 254 628.5 0 254 159 271 628.5 0 271 160 235 628.5 0 235 161 125 628.5 0 125 162 114 628.5 0 114 163 103 628.5 0 103 164 316 628.5 0 316 165 318 628.5 0 318 166 217 628.5 0 217 167 100 628.5 0 100 168 126 628.5 0 126 169 120 628.5 0 120 170 125 628.5 0 125 171 134 628.5 0 134 172 107 628.5 0 107 173 106 628.5 0 106 174 136 628.5 0 136 175 120 628.5 0 120 176 83 628.5 0 83 177 142 628.5 0 142 178 143 628.5 0 143 179 166 628.5 0 166 180 143 628.5 0 143 181 199 628.5 0 199 182 1279 628.5 0 1279 183 637 628.5 0 637 184 829 628.5 0 829 185 104 628.5 0 104 186 83 628.5 0 83 187 102 628.5 0 102 188 110 628.5 0 110 189 114 628.5 0 114 190 104 628.5 0 104 191 105 628.5 0 105 192 115 628.5 0 115 193 109 628.5 0 109 194 137 628.5 0 137 195 129 628.5 0 129 196 112 628.5 0 112 197 98 628.5 0 98 198 118 628.5 0 118 199 134 628.5 0 134 200 137 628.5 0 137 201 149 628.5 0 149 202 120 628.5 0 120 203 130 628.5 0 130 204 127 628.5 0 127 205 130 628.5 0 130 206 142 628.5 0 142 207 150 628.5 0 150 208 200 628.5 0 200 209 150 628.5 0 150 210 125 628.5 0 125 211 151 628.5 0 151 212 152 628.5 0 152 213 152 628.5 0 152 214 91 628.5 0 91 215 126 628.5 0 126 216 131 628.5 0 131 217 109 628.5 0 109 218 130 628.5 0 130 219 108 628.5 0 108 220 167 628.5 0 167 221 152 628.5 0 152 222 122 628.5 0 122 223 130 628.5 0 130 224 164 628.5 0 164 225 153 628.5 0 153 226 149 628.5 0 149 227 151 628.5 0 151 228 137 628.5 0 137 229 129 628.5 0 129 230 117 628.5 0 117 231 179 628.5 0 179 232 115 628.5 0 115 233 163 628.5 0 163 234 127 628.5 0 127 235 142 628.5 0 142 236 119 628.5 0 119 237 142 628.5 0 142 238 165 628.5 0 165 239 141 628.5 0 141 240 127 628.5 0 127 241 135 628.5 0 135 242 140 628.5 0 140 243 122 628.5 0 122 244 121 628.5 0 121 245 109 628.5 0 109 246 105 628.5 0 105 247 137 628.5 0 137 248 156 628.5 0 156 249 134 628.5 0 134 250 136 628.5 0 136 251 138 628.5 0 138 252 166 628.5 0 166 253 390 628.5 0 390 254 791 628.5 0 791 255 1143 628.5 0 1143 256 276 628.5 0 276 257 148 628.5 0 148 258 121 628.5 0 121 259 127 628.5 0 127 260 188 628.5 0 188 261 223 628.5 0 223 262 129 628.5 0 129 263 105 628.5 0 105 264 165 628.5 0 165 265 169 628.5 0 169 266 417 628.5 0 417 267 99 628.5 0 99 268 111 628.5 0 111 269 102 628.5 0 102 270 88 628.5 0 88 271 122 628.5 0 122 272 115 628.5 0 115 273 115 628.5 0 115 274 135 628.5 0 135 275 150 628.5 0 150 276 136 628.5 0 136 277 109 628.5 0 109 278 115 628.5 0 115 279 103 628.5 0 103 280 131 628.5 0 131 281 110 628.5 0 110 282 110 628.5 0 110 283 112 628.5 0 112 284 144 628.5 0 144 285 107 628.5 0 107 286 108 628.5 0 108 287 103 628.5 0 103 288 103 628.5 0 103 289 145 628.5 0 145 290 102 628.5 0 102 291 97 628.5 0 97 292 108 628.5 0 108 293 115 628.5 0 115 294 114 628.5 0 114 295 100 628.5 0 100 296 97 628.5 0 97 297 123 628.5 0 123 298 118 628.5 0 118 299 118 628.5 0 118 300 96 628.5 0 96 301 121 628.5 0 121 Finished in 8.98 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 207,974 Reads with adapters: 77,080 (37.1%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 77,041 (37.0%) Total basepairs processed: 56,514,296 bp Total written (filtered): 21,255,843 bp (37.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 77080 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 13 0.0 3 0 0 13 16 363 0.0 3 0 360 3 17 76395 0.0 3 75056 1296 39 4 18 156 0.0 3 12 140 4 19 26 0.0 3 1 1 21 3 20 26 0.0 3 0 0 0 26 21 3 0.0 3 0 0 0 3 23 1 0.0 3 1 25 8 0.0 3 0 0 0 8 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 6 0.0 3 0 0 0 6 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 67 10 0.0 3 0 0 0 10 89 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 8 0.0 3 0 0 0 8 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 1 136 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 172 6 0.0 3 0 0 0 6 175 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 11 0.0 3 0 0 1 10 215 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.70 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 77,041 Reads with adapters: 758 (1.0%) Reads written (passing filters): 77,041 (100.0%) Total basepairs processed: 21,255,843 bp Total written (filtered): 21,253,331 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 758 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.1% C: 6.7% G: 30.2% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 527 1203.8 0 527 4 230 300.9 0 230 11 1 0.0 1 0 1