This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.29 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 60,495 (2.3%) Reads that were too short: 11,803 (0.5%) Reads written (passing filters): 48,692 (1.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 12,684,450 bp (1.6%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 60495 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 688 628.5 0 688 7 17283 628.5 0 17283 8 15691 628.5 0 15691 9 69 628.5 0 69 10 47 628.5 0 47 11 57 628.5 0 57 12 40 628.5 0 40 13 63 628.5 0 63 14 49 628.5 0 49 15 54 628.5 0 54 16 101 628.5 0 101 17 54 628.5 0 54 18 67 628.5 0 67 19 58 628.5 0 58 20 38 628.5 0 38 21 33 628.5 0 33 22 46 628.5 0 46 23 50 628.5 0 50 24 51 628.5 0 51 25 61 628.5 0 61 26 111 628.5 0 111 27 88 628.5 0 88 28 273 628.5 0 273 29 34 628.5 0 34 30 41 628.5 0 41 31 46 628.5 0 46 32 58 628.5 0 58 33 41 628.5 0 41 34 32 628.5 0 32 35 45 628.5 0 45 36 126 628.5 0 126 37 65 628.5 0 65 38 36 628.5 0 36 39 53 628.5 0 53 40 39 628.5 0 39 41 43 628.5 0 43 42 58 628.5 0 58 43 44 628.5 0 44 44 41 628.5 0 41 45 38 628.5 0 38 46 48 628.5 0 48 47 46 628.5 0 46 48 41 628.5 0 41 49 41 628.5 0 41 50 44 628.5 0 44 51 60 628.5 0 60 52 35 628.5 0 35 53 33 628.5 0 33 54 48 628.5 0 48 55 35 628.5 0 35 56 35 628.5 0 35 57 32 628.5 0 32 58 38 628.5 0 38 59 46 628.5 0 46 60 39 628.5 0 39 61 46 628.5 0 46 62 40 628.5 0 40 63 45 628.5 0 45 64 43 628.5 0 43 65 53 628.5 0 53 66 49 628.5 0 49 67 49 628.5 0 49 68 45 628.5 0 45 69 49 628.5 0 49 70 36 628.5 0 36 71 40 628.5 0 40 72 44 628.5 0 44 73 38 628.5 0 38 74 48 628.5 0 48 75 63 628.5 0 63 76 51 628.5 0 51 77 75 628.5 0 75 78 48 628.5 0 48 79 49 628.5 0 49 80 46 628.5 0 46 81 59 628.5 0 59 82 61 628.5 0 61 83 50 628.5 0 50 84 49 628.5 0 49 85 53 628.5 0 53 86 43 628.5 0 43 87 45 628.5 0 45 88 25 628.5 0 25 89 43 628.5 0 43 90 42 628.5 0 42 91 60 628.5 0 60 92 59 628.5 0 59 93 32 628.5 0 32 94 57 628.5 0 57 95 53 628.5 0 53 96 66 628.5 0 66 97 54 628.5 0 54 98 80 628.5 0 80 99 84 628.5 0 84 100 101 628.5 0 101 101 64 628.5 0 64 102 68 628.5 0 68 103 60 628.5 0 60 104 48 628.5 0 48 105 56 628.5 0 56 106 80 628.5 0 80 107 73 628.5 0 73 108 310 628.5 0 310 109 391 628.5 0 391 110 1072 628.5 0 1072 111 116 628.5 0 116 112 103 628.5 0 103 113 86 628.5 0 86 114 178 628.5 0 178 115 90 628.5 0 90 116 144 628.5 0 144 117 69 628.5 0 69 118 42 628.5 0 42 119 67 628.5 0 67 120 75 628.5 0 75 121 47 628.5 0 47 122 91 628.5 0 91 123 76 628.5 0 76 124 57 628.5 0 57 125 59 628.5 0 59 126 61 628.5 0 61 127 52 628.5 0 52 128 62 628.5 0 62 129 95 628.5 0 95 130 71 628.5 0 71 131 113 628.5 0 113 132 76 628.5 0 76 133 80 628.5 0 80 134 85 628.5 0 85 135 89 628.5 0 89 136 109 628.5 0 109 137 84 628.5 0 84 138 84 628.5 0 84 139 114 628.5 0 114 140 164 628.5 0 164 141 140 628.5 0 140 142 115 628.5 0 115 143 99 628.5 0 99 144 90 628.5 0 90 145 126 628.5 0 126 146 76 628.5 0 76 147 73 628.5 0 73 148 71 628.5 0 71 149 66 628.5 0 66 150 76 628.5 0 76 151 48 628.5 0 48 152 56 628.5 0 56 153 71 628.5 0 71 154 61 628.5 0 61 155 145 628.5 0 145 156 69 628.5 0 69 157 120 628.5 0 120 158 119 628.5 0 119 159 78 628.5 0 78 160 87 628.5 0 87 161 78 628.5 0 78 162 86 628.5 0 86 163 186 628.5 0 186 164 99 628.5 0 99 165 147 628.5 0 147 166 100 628.5 0 100 167 119 628.5 0 119 168 62 628.5 0 62 169 95 628.5 0 95 170 81 628.5 0 81 171 70 628.5 0 70 172 81 628.5 0 81 173 68 628.5 0 68 174 50 628.5 0 50 175 49 628.5 0 49 176 83 628.5 0 83 177 57 628.5 0 57 178 110 628.5 0 110 179 86 628.5 0 86 180 97 628.5 0 97 181 64 628.5 0 64 182 101 628.5 0 101 183 72 628.5 0 72 184 82 628.5 0 82 185 59 628.5 0 59 186 75 628.5 0 75 187 83 628.5 0 83 188 78 628.5 0 78 189 64 628.5 0 64 190 103 628.5 0 103 191 77 628.5 0 77 192 87 628.5 0 87 193 67 628.5 0 67 194 61 628.5 0 61 195 100 628.5 0 100 196 69 628.5 0 69 197 69 628.5 0 69 198 65 628.5 0 65 199 67 628.5 0 67 200 76 628.5 0 76 201 116 628.5 0 116 202 115 628.5 0 115 203 102 628.5 0 102 204 117 628.5 0 117 205 86 628.5 0 86 206 85 628.5 0 85 207 89 628.5 0 89 208 145 628.5 0 145 209 146 628.5 0 146 210 230 628.5 0 230 211 130 628.5 0 130 212 71 628.5 0 71 213 83 628.5 0 83 214 67 628.5 0 67 215 80 628.5 0 80 216 102 628.5 0 102 217 80 628.5 0 80 218 64 628.5 0 64 219 74 628.5 0 74 220 86 628.5 0 86 221 73 628.5 0 73 222 117 628.5 0 117 223 218 628.5 0 218 224 276 628.5 0 276 225 126 628.5 0 126 226 154 628.5 0 154 227 74 628.5 0 74 228 46 628.5 0 46 229 63 628.5 0 63 230 51 628.5 0 51 231 48 628.5 0 48 232 76 628.5 0 76 233 60 628.5 0 60 234 82 628.5 0 82 235 57 628.5 0 57 236 56 628.5 0 56 237 71 628.5 0 71 238 75 628.5 0 75 239 65 628.5 0 65 240 97 628.5 0 97 241 80 628.5 0 80 242 96 628.5 0 96 243 167 628.5 0 167 244 251 628.5 0 251 245 164 628.5 0 164 246 218 628.5 0 218 247 73 628.5 0 73 248 68 628.5 0 68 249 56 628.5 0 56 250 59 628.5 0 59 251 74 628.5 0 74 252 100 628.5 0 100 253 262 628.5 0 262 254 250 628.5 0 250 255 488 628.5 0 488 256 104 628.5 0 104 257 77 628.5 0 77 258 305 628.5 0 305 259 324 628.5 0 324 260 430 628.5 0 430 261 55 628.5 0 55 262 141 628.5 0 141 263 239 628.5 0 239 264 293 628.5 0 293 265 223 628.5 0 223 266 67 628.5 0 67 267 54 628.5 0 54 268 49 628.5 0 49 269 74 628.5 0 74 270 77 628.5 0 77 271 130 628.5 0 130 272 125 628.5 0 125 273 89 628.5 0 89 274 56 628.5 0 56 275 73 628.5 0 73 276 78 628.5 0 78 277 71 628.5 0 71 278 80 628.5 0 80 279 53 628.5 0 53 280 63 628.5 0 63 281 90 628.5 0 90 282 89 628.5 0 89 283 158 628.5 0 158 284 142 628.5 0 142 285 163 628.5 0 163 286 109 628.5 0 109 287 71 628.5 0 71 288 61 628.5 0 61 289 75 628.5 0 75 290 113 628.5 0 113 291 198 628.5 0 198 292 207 628.5 0 207 293 71 628.5 0 71 294 90 628.5 0 90 295 53 628.5 0 53 296 83 628.5 0 83 297 94 628.5 0 94 298 107 628.5 0 107 299 86 628.5 0 86 300 74 628.5 0 74 301 96 628.5 0 96 Finished in 2.45 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 48,692 Reads with adapters: 17,022 (35.0%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 17,003 (34.9%) Total basepairs processed: 12,684,450 bp Total written (filtered): 4,545,413 bp (35.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17022 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 6 0.0 3 0 0 3 2 1 19 212 0.0 3 0 147 28 22 15 20 15727 0.0 4 14331 1083 223 72 18 21 38 0.0 4 4 29 1 3 1 22 20 0.0 4 9 4 4 2 1 23 81 0.0 4 0 0 1 56 24 24 1 0.0 4 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 34 0.0 4 0 0 0 0 34 29 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 20 0.0 4 0 0 0 0 20 127 830 0.0 4 0 0 0 1 829 128 14 0.0 4 0 0 0 1 13 130 5 0.0 4 0 0 0 0 5 143 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 1 211 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 253 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 17,003 Reads with adapters: 4,096 (24.1%) Reads written (passing filters): 17,003 (100.0%) Total basepairs processed: 4,545,413 bp Total written (filtered): 4,499,493 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4096 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 7.9% G: 87.0% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 26 265.7 0 26 4 14 66.4 0 14 7 1 1.0 0 1 9 22 0.1 0 1 21 10 501 0.0 1 53 448 11 3481 0.0 1 122 3359 12 39 0.0 1 0 39 13 1 0.0 1 0 1 14 2 0.0 1 0 2 151 2 0.0 1 0 2 170 1 0.0 1 1 209 3 0.0 1 2 1 224 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.