This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.49 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 51,796 (2.0%) Reads that were too short: 7,990 (0.3%) Reads written (passing filters): 43,806 (1.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,934,867 bp (1.5%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 51796 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 430 628.5 0 430 7 17492 628.5 0 17492 8 17552 628.5 0 17552 9 53 628.5 0 53 10 41 628.5 0 41 11 31 628.5 0 31 12 23 628.5 0 23 13 41 628.5 0 41 14 16 628.5 0 16 15 27 628.5 0 27 16 37 628.5 0 37 17 32 628.5 0 32 18 28 628.5 0 28 19 20 628.5 0 20 20 22 628.5 0 22 21 22 628.5 0 22 22 26 628.5 0 26 23 22 628.5 0 22 24 22 628.5 0 22 25 26 628.5 0 26 26 26 628.5 0 26 27 27 628.5 0 27 28 39 628.5 0 39 29 27 628.5 0 27 30 21 628.5 0 21 31 35 628.5 0 35 32 29 628.5 0 29 33 29 628.5 0 29 34 28 628.5 0 28 35 15 628.5 0 15 36 120 628.5 0 120 37 28 628.5 0 28 38 39 628.5 0 39 39 26 628.5 0 26 40 24 628.5 0 24 41 24 628.5 0 24 42 27 628.5 0 27 43 19 628.5 0 19 44 22 628.5 0 22 45 29 628.5 0 29 46 18 628.5 0 18 47 28 628.5 0 28 48 25 628.5 0 25 49 29 628.5 0 29 50 23 628.5 0 23 51 35 628.5 0 35 52 23 628.5 0 23 53 30 628.5 0 30 54 27 628.5 0 27 55 25 628.5 0 25 56 14 628.5 0 14 57 18 628.5 0 18 58 25 628.5 0 25 59 26 628.5 0 26 60 21 628.5 0 21 61 20 628.5 0 20 62 21 628.5 0 21 63 23 628.5 0 23 64 21 628.5 0 21 65 24 628.5 0 24 66 37 628.5 0 37 67 24 628.5 0 24 68 31 628.5 0 31 69 24 628.5 0 24 70 21 628.5 0 21 71 28 628.5 0 28 72 26 628.5 0 26 73 19 628.5 0 19 74 19 628.5 0 19 75 36 628.5 0 36 76 26 628.5 0 26 77 22 628.5 0 22 78 25 628.5 0 25 79 31 628.5 0 31 80 45 628.5 0 45 81 26 628.5 0 26 82 35 628.5 0 35 83 40 628.5 0 40 84 34 628.5 0 34 85 56 628.5 0 56 86 40 628.5 0 40 87 28 628.5 0 28 88 27 628.5 0 27 89 30 628.5 0 30 90 37 628.5 0 37 91 51 628.5 0 51 92 33 628.5 0 33 93 22 628.5 0 22 94 40 628.5 0 40 95 31 628.5 0 31 96 35 628.5 0 35 97 39 628.5 0 39 98 37 628.5 0 37 99 41 628.5 0 41 100 45 628.5 0 45 101 41 628.5 0 41 102 39 628.5 0 39 103 47 628.5 0 47 104 30 628.5 0 30 105 31 628.5 0 31 106 50 628.5 0 50 107 48 628.5 0 48 108 94 628.5 0 94 109 138 628.5 0 138 110 263 628.5 0 263 111 63 628.5 0 63 112 56 628.5 0 56 113 54 628.5 0 54 114 79 628.5 0 79 115 59 628.5 0 59 116 84 628.5 0 84 117 53 628.5 0 53 118 41 628.5 0 41 119 51 628.5 0 51 120 36 628.5 0 36 121 37 628.5 0 37 122 55 628.5 0 55 123 46 628.5 0 46 124 49 628.5 0 49 125 27 628.5 0 27 126 39 628.5 0 39 127 48 628.5 0 48 128 48 628.5 0 48 129 53 628.5 0 53 130 40 628.5 0 40 131 92 628.5 0 92 132 38 628.5 0 38 133 50 628.5 0 50 134 57 628.5 0 57 135 54 628.5 0 54 136 70 628.5 0 70 137 55 628.5 0 55 138 53 628.5 0 53 139 53 628.5 0 53 140 81 628.5 0 81 141 54 628.5 0 54 142 76 628.5 0 76 143 60 628.5 0 60 144 53 628.5 0 53 145 57 628.5 0 57 146 38 628.5 0 38 147 37 628.5 0 37 148 32 628.5 0 32 149 38 628.5 0 38 150 49 628.5 0 49 151 44 628.5 0 44 152 38 628.5 0 38 153 46 628.5 0 46 154 64 628.5 0 64 155 75 628.5 0 75 156 55 628.5 0 55 157 67 628.5 0 67 158 66 628.5 0 66 159 44 628.5 0 44 160 51 628.5 0 51 161 38 628.5 0 38 162 57 628.5 0 57 163 97 628.5 0 97 164 45 628.5 0 45 165 60 628.5 0 60 166 60 628.5 0 60 167 58 628.5 0 58 168 44 628.5 0 44 169 52 628.5 0 52 170 49 628.5 0 49 171 45 628.5 0 45 172 42 628.5 0 42 173 44 628.5 0 44 174 37 628.5 0 37 175 43 628.5 0 43 176 62 628.5 0 62 177 44 628.5 0 44 178 73 628.5 0 73 179 49 628.5 0 49 180 42 628.5 0 42 181 53 628.5 0 53 182 70 628.5 0 70 183 55 628.5 0 55 184 60 628.5 0 60 185 54 628.5 0 54 186 49 628.5 0 49 187 55 628.5 0 55 188 48 628.5 0 48 189 57 628.5 0 57 190 50 628.5 0 50 191 43 628.5 0 43 192 63 628.5 0 63 193 38 628.5 0 38 194 43 628.5 0 43 195 54 628.5 0 54 196 55 628.5 0 55 197 56 628.5 0 56 198 34 628.5 0 34 199 44 628.5 0 44 200 51 628.5 0 51 201 73 628.5 0 73 202 67 628.5 0 67 203 59 628.5 0 59 204 62 628.5 0 62 205 38 628.5 0 38 206 49 628.5 0 49 207 54 628.5 0 54 208 90 628.5 0 90 209 98 628.5 0 98 210 103 628.5 0 103 211 104 628.5 0 104 212 47 628.5 0 47 213 53 628.5 0 53 214 38 628.5 0 38 215 45 628.5 0 45 216 53 628.5 0 53 217 52 628.5 0 52 218 31 628.5 0 31 219 47 628.5 0 47 220 49 628.5 0 49 221 35 628.5 0 35 222 62 628.5 0 62 223 91 628.5 0 91 224 116 628.5 0 116 225 62 628.5 0 62 226 74 628.5 0 74 227 47 628.5 0 47 228 41 628.5 0 41 229 31 628.5 0 31 230 46 628.5 0 46 231 35 628.5 0 35 232 51 628.5 0 51 233 30 628.5 0 30 234 32 628.5 0 32 235 53 628.5 0 53 236 41 628.5 0 41 237 49 628.5 0 49 238 46 628.5 0 46 239 37 628.5 0 37 240 53 628.5 0 53 241 62 628.5 0 62 242 74 628.5 0 74 243 105 628.5 0 105 244 138 628.5 0 138 245 108 628.5 0 108 246 124 628.5 0 124 247 49 628.5 0 49 248 50 628.5 0 50 249 47 628.5 0 47 250 52 628.5 0 52 251 55 628.5 0 55 252 86 628.5 0 86 253 224 628.5 0 224 254 186 628.5 0 186 255 359 628.5 0 359 256 78 628.5 0 78 257 59 628.5 0 59 258 369 628.5 0 369 259 421 628.5 0 421 260 615 628.5 0 615 261 52 628.5 0 52 262 74 628.5 0 74 263 84 628.5 0 84 264 99 628.5 0 99 265 80 628.5 0 80 266 36 628.5 0 36 267 22 628.5 0 22 268 36 628.5 0 36 269 42 628.5 0 42 270 47 628.5 0 47 271 88 628.5 0 88 272 81 628.5 0 81 273 57 628.5 0 57 274 46 628.5 0 46 275 49 628.5 0 49 276 49 628.5 0 49 277 41 628.5 0 41 278 57 628.5 0 57 279 48 628.5 0 48 280 45 628.5 0 45 281 70 628.5 0 70 282 52 628.5 0 52 283 85 628.5 0 85 284 76 628.5 0 76 285 73 628.5 0 73 286 62 628.5 0 62 287 56 628.5 0 56 288 50 628.5 0 50 289 48 628.5 0 48 290 68 628.5 0 68 291 147 628.5 0 147 292 117 628.5 0 117 293 36 628.5 0 36 294 47 628.5 0 47 295 47 628.5 0 47 296 60 628.5 0 60 297 70 628.5 0 70 298 87 628.5 0 87 299 65 628.5 0 65 300 54 628.5 0 54 301 55 628.5 0 55 Finished in 2.07 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 43,806 Reads with adapters: 17,336 (39.6%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 17,330 (39.6%) Total basepairs processed: 11,934,867 bp Total written (filtered): 4,799,679 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17336 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 93 0.0 3 0 91 2 17 17175 0.0 3 16839 327 8 1 18 36 0.0 3 2 33 1 19 6 0.0 3 0 0 6 20 12 0.0 3 0 0 0 12 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 17,330 Reads with adapters: 37 (0.2%) Reads written (passing filters): 17,330 (100.0%) Total basepairs processed: 4,799,679 bp Total written (filtered): 4,799,558 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.9% C: 13.5% G: 13.5% T: 8.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 31 270.8 0 31 4 4 67.7 0 4 6 2 4.2 0 2