This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.25 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 141,687 (5.5%) Reads that were too short: 45,871 (1.8%) Reads written (passing filters): 95,816 (3.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 24,927,263 bp (3.2%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 141687 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4274 628.5 0 4274 7 26666 628.5 0 26666 8 1023 628.5 0 1023 9 33070 628.5 0 33070 10 105 628.5 0 105 11 78 628.5 0 78 12 74 628.5 0 74 13 97 628.5 0 97 14 80 628.5 0 80 15 68 628.5 0 68 16 52 628.5 0 52 17 137 628.5 0 137 18 229 628.5 0 229 19 170 628.5 0 170 20 54 628.5 0 54 21 76 628.5 0 76 22 80 628.5 0 80 23 90 628.5 0 90 24 57 628.5 0 57 25 71 628.5 0 71 26 67 628.5 0 67 27 70 628.5 0 70 28 58 628.5 0 58 29 58 628.5 0 58 30 80 628.5 0 80 31 89 628.5 0 89 32 100 628.5 0 100 33 87 628.5 0 87 34 78 628.5 0 78 35 50 628.5 0 50 36 68 628.5 0 68 37 82 628.5 0 82 38 83 628.5 0 83 39 82 628.5 0 82 40 98 628.5 0 98 41 100 628.5 0 100 42 109 628.5 0 109 43 92 628.5 0 92 44 82 628.5 0 82 45 103 628.5 0 103 46 99 628.5 0 99 47 407 628.5 0 407 48 547 628.5 0 547 49 888 628.5 0 888 50 142 628.5 0 142 51 76 628.5 0 76 52 83 628.5 0 83 53 81 628.5 0 81 54 85 628.5 0 85 55 84 628.5 0 84 56 72 628.5 0 72 57 74 628.5 0 74 58 112 628.5 0 112 59 207 628.5 0 207 60 296 628.5 0 296 61 250 628.5 0 250 62 96 628.5 0 96 63 82 628.5 0 82 64 93 628.5 0 93 65 73 628.5 0 73 66 76 628.5 0 76 67 69 628.5 0 69 68 81 628.5 0 81 69 81 628.5 0 81 70 131 628.5 0 131 71 137 628.5 0 137 72 173 628.5 0 173 73 68 628.5 0 68 74 73 628.5 0 73 75 77 628.5 0 77 76 105 628.5 0 105 77 302 628.5 0 302 78 269 628.5 0 269 79 281 628.5 0 281 80 90 628.5 0 90 81 115 628.5 0 115 82 281 628.5 0 281 83 140 628.5 0 140 84 620 628.5 0 620 85 566 628.5 0 566 86 673 628.5 0 673 87 73 628.5 0 73 88 62 628.5 0 62 89 74 628.5 0 74 90 52 628.5 0 52 91 95 628.5 0 95 92 57 628.5 0 57 93 90 628.5 0 90 94 60 628.5 0 60 95 45 628.5 0 45 96 83 628.5 0 83 97 99 628.5 0 99 98 47 628.5 0 47 99 76 628.5 0 76 100 91 628.5 0 91 101 53 628.5 0 53 102 43 628.5 0 43 103 52 628.5 0 52 104 57 628.5 0 57 105 72 628.5 0 72 106 73 628.5 0 73 107 88 628.5 0 88 108 67 628.5 0 67 109 90 628.5 0 90 110 76 628.5 0 76 111 75 628.5 0 75 112 375 628.5 0 375 113 386 628.5 0 386 114 406 628.5 0 406 115 89 628.5 0 89 116 80 628.5 0 80 117 155 628.5 0 155 118 163 628.5 0 163 119 220 628.5 0 220 120 97 628.5 0 97 121 67 628.5 0 67 122 79 628.5 0 79 123 65 628.5 0 65 124 70 628.5 0 70 125 81 628.5 0 81 126 104 628.5 0 104 127 127 628.5 0 127 128 199 628.5 0 199 129 256 628.5 0 256 130 134 628.5 0 134 131 184 628.5 0 184 132 367 628.5 0 367 133 561 628.5 0 561 134 718 628.5 0 718 135 330 628.5 0 330 136 228 628.5 0 228 137 127 628.5 0 127 138 146 628.5 0 146 139 189 628.5 0 189 140 146 628.5 0 146 141 440 628.5 0 440 142 2121 628.5 0 2121 143 2719 628.5 0 2719 144 80 628.5 0 80 145 67 628.5 0 67 146 88 628.5 0 88 147 99 628.5 0 99 148 114 628.5 0 114 149 103 628.5 0 103 150 93 628.5 0 93 151 126 628.5 0 126 152 343 628.5 0 343 153 565 628.5 0 565 154 523 628.5 0 523 155 215 628.5 0 215 156 58 628.5 0 58 157 71 628.5 0 71 158 71 628.5 0 71 159 87 628.5 0 87 160 58 628.5 0 58 161 54 628.5 0 54 162 67 628.5 0 67 163 70 628.5 0 70 164 94 628.5 0 94 165 49 628.5 0 49 166 80 628.5 0 80 167 57 628.5 0 57 168 65 628.5 0 65 169 92 628.5 0 92 170 78 628.5 0 78 171 88 628.5 0 88 172 75 628.5 0 75 173 85 628.5 0 85 174 77 628.5 0 77 175 65 628.5 0 65 176 62 628.5 0 62 177 62 628.5 0 62 178 58 628.5 0 58 179 74 628.5 0 74 180 61 628.5 0 61 181 66 628.5 0 66 182 70 628.5 0 70 183 67 628.5 0 67 184 90 628.5 0 90 185 90 628.5 0 90 186 90 628.5 0 90 187 117 628.5 0 117 188 93 628.5 0 93 189 110 628.5 0 110 190 125 628.5 0 125 191 129 628.5 0 129 192 89 628.5 0 89 193 102 628.5 0 102 194 76 628.5 0 76 195 81 628.5 0 81 196 95 628.5 0 95 197 112 628.5 0 112 198 87 628.5 0 87 199 109 628.5 0 109 200 120 628.5 0 120 201 103 628.5 0 103 202 90 628.5 0 90 203 117 628.5 0 117 204 93 628.5 0 93 205 92 628.5 0 92 206 118 628.5 0 118 207 106 628.5 0 106 208 82 628.5 0 82 209 87 628.5 0 87 210 101 628.5 0 101 211 120 628.5 0 120 212 190 628.5 0 190 213 185 628.5 0 185 214 266 628.5 0 266 215 188 628.5 0 188 216 171 628.5 0 171 217 190 628.5 0 190 218 304 628.5 0 304 219 341 628.5 0 341 220 225 628.5 0 225 221 103 628.5 0 103 222 88 628.5 0 88 223 57 628.5 0 57 224 92 628.5 0 92 225 145 628.5 0 145 226 139 628.5 0 139 227 133 628.5 0 133 228 123 628.5 0 123 229 122 628.5 0 122 230 231 628.5 0 231 231 177 628.5 0 177 232 203 628.5 0 203 233 163 628.5 0 163 234 230 628.5 0 230 235 868 628.5 0 868 236 1150 628.5 0 1150 237 1335 628.5 0 1335 238 582 628.5 0 582 239 734 628.5 0 734 240 534 628.5 0 534 241 199 628.5 0 199 242 109 628.5 0 109 243 168 628.5 0 168 244 197 628.5 0 197 245 362 628.5 0 362 246 468 628.5 0 468 247 558 628.5 0 558 248 317 628.5 0 317 249 194 628.5 0 194 250 219 628.5 0 219 251 252 628.5 0 252 252 512 628.5 0 512 253 350 628.5 0 350 254 525 628.5 0 525 255 660 628.5 0 660 256 1383 628.5 0 1383 257 1146 628.5 0 1146 258 850 628.5 0 850 259 143 628.5 0 143 260 171 628.5 0 171 261 183 628.5 0 183 262 112 628.5 0 112 263 137 628.5 0 137 264 122 628.5 0 122 265 96 628.5 0 96 266 119 628.5 0 119 267 101 628.5 0 101 268 103 628.5 0 103 269 104 628.5 0 104 270 113 628.5 0 113 271 114 628.5 0 114 272 141 628.5 0 141 273 294 628.5 0 294 274 332 628.5 0 332 275 192 628.5 0 192 276 120 628.5 0 120 277 125 628.5 0 125 278 160 628.5 0 160 279 196 628.5 0 196 280 199 628.5 0 199 281 144 628.5 0 144 282 231 628.5 0 231 283 425 628.5 0 425 284 883 628.5 0 883 285 1149 628.5 0 1149 286 557 628.5 0 557 287 579 628.5 0 579 288 384 628.5 0 384 289 264 628.5 0 264 290 199 628.5 0 199 291 297 628.5 0 297 292 258 628.5 0 258 293 324 628.5 0 324 294 306 628.5 0 306 295 420 628.5 0 420 296 508 628.5 0 508 297 598 628.5 0 598 298 5238 628.5 0 5238 299 6642 628.5 0 6642 300 3427 628.5 0 3427 301 917 628.5 0 917 Finished in 4.92 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 95,816 Reads with adapters: 28,880 (30.1%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 28,836 (30.1%) Total basepairs processed: 24,927,263 bp Total written (filtered): 7,868,494 bp (31.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28880 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 3 0.0 3 0 0 0 0 3 17 20 0.0 3 0 0 0 3 17 18 34 0.0 3 0 0 14 12 8 19 538 0.0 3 0 319 84 87 48 20 26569 0.0 4 21369 3417 1168 429 186 21 145 0.0 4 22 70 23 13 17 22 1056 0.0 4 902 115 21 11 7 23 165 0.0 4 2 0 0 72 91 24 3 0.0 4 1 0 0 0 2 25 15 0.0 4 0 0 0 11 4 31 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 87 3 0.0 4 0 0 0 0 3 92 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 117 5 0.0 4 0 0 0 0 5 120 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 242 0.0 4 0 0 0 0 242 128 1 0.0 4 0 0 0 0 1 129 16 0.0 4 0 0 0 0 16 130 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 1 188 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 4 0.0 4 0 0 0 0 4 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 243 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 3 0.0 4 0 0 0 1 2 274 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.00 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 28,836 Reads with adapters: 1,930 (6.7%) Reads written (passing filters): 28,836 (100.0%) Total basepairs processed: 7,868,494 bp Total written (filtered): 7,845,393 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1930 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.9% C: 18.3% G: 71.6% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 198 450.6 0 198 4 41 112.6 0 41 5 5 28.2 0 5 6 1 7.0 0 1 8 1 0.4 0 1 9 58 0.1 0 5 53 10 217 0.0 1 16 201 11 734 0.0 1 39 695 12 605 0.0 1 18 587 13 40 0.0 1 0 40 14 5 0.0 1 0 5 15 2 0.0 1 0 2 95 1 0.0 1 0 1 152 16 0.0 1 11 5 153 1 0.0 1 0 1 171 1 0.0 1 1 177 1 0.0 1 1 210 3 0.0 1 2 1