This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.76 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 115,729 (4.5%) Reads that were too short: 19,531 (0.8%) Reads written (passing filters): 96,198 (3.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 25,473,254 bp (3.3%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 115729 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4012 628.5 0 4012 7 33605 628.5 0 33605 8 854 628.5 0 854 9 31546 628.5 0 31546 10 120 628.5 0 120 11 103 628.5 0 103 12 92 628.5 0 92 13 110 628.5 0 110 14 97 628.5 0 97 15 92 628.5 0 92 16 63 628.5 0 63 17 90 628.5 0 90 18 118 628.5 0 118 19 93 628.5 0 93 20 85 628.5 0 85 21 108 628.5 0 108 22 76 628.5 0 76 23 91 628.5 0 91 24 91 628.5 0 91 25 79 628.5 0 79 26 81 628.5 0 81 27 98 628.5 0 98 28 78 628.5 0 78 29 74 628.5 0 74 30 73 628.5 0 73 31 55 628.5 0 55 32 80 628.5 0 80 33 81 628.5 0 81 34 85 628.5 0 85 35 84 628.5 0 84 36 64 628.5 0 64 37 86 628.5 0 86 38 79 628.5 0 79 39 103 628.5 0 103 40 108 628.5 0 108 41 92 628.5 0 92 42 123 628.5 0 123 43 81 628.5 0 81 44 82 628.5 0 82 45 89 628.5 0 89 46 84 628.5 0 84 47 141 628.5 0 141 48 179 628.5 0 179 49 164 628.5 0 164 50 99 628.5 0 99 51 74 628.5 0 74 52 153 628.5 0 153 53 99 628.5 0 99 54 104 628.5 0 104 55 135 628.5 0 135 56 106 628.5 0 106 57 85 628.5 0 85 58 101 628.5 0 101 59 146 628.5 0 146 60 182 628.5 0 182 61 200 628.5 0 200 62 109 628.5 0 109 63 91 628.5 0 91 64 79 628.5 0 79 65 95 628.5 0 95 66 85 628.5 0 85 67 103 628.5 0 103 68 92 628.5 0 92 69 108 628.5 0 108 70 133 628.5 0 133 71 113 628.5 0 113 72 173 628.5 0 173 73 105 628.5 0 105 74 78 628.5 0 78 75 92 628.5 0 92 76 121 628.5 0 121 77 135 628.5 0 135 78 108 628.5 0 108 79 129 628.5 0 129 80 112 628.5 0 112 81 101 628.5 0 101 82 122 628.5 0 122 83 112 628.5 0 112 84 380 628.5 0 380 85 305 628.5 0 305 86 417 628.5 0 417 87 76 628.5 0 76 88 71 628.5 0 71 89 122 628.5 0 122 90 54 628.5 0 54 91 94 628.5 0 94 92 94 628.5 0 94 93 84 628.5 0 84 94 117 628.5 0 117 95 78 628.5 0 78 96 76 628.5 0 76 97 97 628.5 0 97 98 53 628.5 0 53 99 78 628.5 0 78 100 82 628.5 0 82 101 93 628.5 0 93 102 102 628.5 0 102 103 53 628.5 0 53 104 58 628.5 0 58 105 82 628.5 0 82 106 115 628.5 0 115 107 115 628.5 0 115 108 93 628.5 0 93 109 113 628.5 0 113 110 82 628.5 0 82 111 89 628.5 0 89 112 434 628.5 0 434 113 430 628.5 0 430 114 480 628.5 0 480 115 70 628.5 0 70 116 70 628.5 0 70 117 106 628.5 0 106 118 91 628.5 0 91 119 110 628.5 0 110 120 71 628.5 0 71 121 72 628.5 0 72 122 81 628.5 0 81 123 91 628.5 0 91 124 81 628.5 0 81 125 78 628.5 0 78 126 92 628.5 0 92 127 124 628.5 0 124 128 153 628.5 0 153 129 123 628.5 0 123 130 126 628.5 0 126 131 136 628.5 0 136 132 186 628.5 0 186 133 261 628.5 0 261 134 331 628.5 0 331 135 190 628.5 0 190 136 130 628.5 0 130 137 83 628.5 0 83 138 97 628.5 0 97 139 124 628.5 0 124 140 150 628.5 0 150 141 414 628.5 0 414 142 1986 628.5 0 1986 143 2611 628.5 0 2611 144 91 628.5 0 91 145 78 628.5 0 78 146 96 628.5 0 96 147 100 628.5 0 100 148 100 628.5 0 100 149 99 628.5 0 99 150 75 628.5 0 75 151 100 628.5 0 100 152 209 628.5 0 209 153 247 628.5 0 247 154 235 628.5 0 235 155 133 628.5 0 133 156 105 628.5 0 105 157 92 628.5 0 92 158 81 628.5 0 81 159 108 628.5 0 108 160 87 628.5 0 87 161 87 628.5 0 87 162 85 628.5 0 85 163 94 628.5 0 94 164 85 628.5 0 85 165 80 628.5 0 80 166 115 628.5 0 115 167 90 628.5 0 90 168 63 628.5 0 63 169 105 628.5 0 105 170 113 628.5 0 113 171 86 628.5 0 86 172 97 628.5 0 97 173 95 628.5 0 95 174 79 628.5 0 79 175 88 628.5 0 88 176 80 628.5 0 80 177 76 628.5 0 76 178 87 628.5 0 87 179 73 628.5 0 73 180 81 628.5 0 81 181 67 628.5 0 67 182 91 628.5 0 91 183 71 628.5 0 71 184 110 628.5 0 110 185 121 628.5 0 121 186 81 628.5 0 81 187 154 628.5 0 154 188 90 628.5 0 90 189 93 628.5 0 93 190 85 628.5 0 85 191 94 628.5 0 94 192 93 628.5 0 93 193 69 628.5 0 69 194 69 628.5 0 69 195 80 628.5 0 80 196 95 628.5 0 95 197 77 628.5 0 77 198 81 628.5 0 81 199 90 628.5 0 90 200 75 628.5 0 75 201 93 628.5 0 93 202 102 628.5 0 102 203 149 628.5 0 149 204 80 628.5 0 80 205 88 628.5 0 88 206 63 628.5 0 63 207 77 628.5 0 77 208 56 628.5 0 56 209 79 628.5 0 79 210 75 628.5 0 75 211 81 628.5 0 81 212 83 628.5 0 83 213 119 628.5 0 119 214 93 628.5 0 93 215 71 628.5 0 71 216 115 628.5 0 115 217 82 628.5 0 82 218 90 628.5 0 90 219 83 628.5 0 83 220 77 628.5 0 77 221 57 628.5 0 57 222 61 628.5 0 61 223 48 628.5 0 48 224 63 628.5 0 63 225 93 628.5 0 93 226 103 628.5 0 103 227 89 628.5 0 89 228 82 628.5 0 82 229 61 628.5 0 61 230 135 628.5 0 135 231 96 628.5 0 96 232 93 628.5 0 93 233 74 628.5 0 74 234 90 628.5 0 90 235 254 628.5 0 254 236 304 628.5 0 304 237 342 628.5 0 342 238 129 628.5 0 129 239 135 628.5 0 135 240 98 628.5 0 98 241 77 628.5 0 77 242 65 628.5 0 65 243 67 628.5 0 67 244 75 628.5 0 75 245 114 628.5 0 114 246 130 628.5 0 130 247 117 628.5 0 117 248 136 628.5 0 136 249 78 628.5 0 78 250 78 628.5 0 78 251 88 628.5 0 88 252 124 628.5 0 124 253 122 628.5 0 122 254 123 628.5 0 123 255 172 628.5 0 172 256 394 628.5 0 394 257 389 628.5 0 389 258 288 628.5 0 288 259 65 628.5 0 65 260 110 628.5 0 110 261 113 628.5 0 113 262 60 628.5 0 60 263 64 628.5 0 64 264 69 628.5 0 69 265 58 628.5 0 58 266 76 628.5 0 76 267 72 628.5 0 72 268 64 628.5 0 64 269 74 628.5 0 74 270 73 628.5 0 73 271 59 628.5 0 59 272 80 628.5 0 80 273 157 628.5 0 157 274 326 628.5 0 326 275 219 628.5 0 219 276 77 628.5 0 77 277 77 628.5 0 77 278 70 628.5 0 70 279 81 628.5 0 81 280 91 628.5 0 91 281 89 628.5 0 89 282 92 628.5 0 92 283 127 628.5 0 127 284 198 628.5 0 198 285 250 628.5 0 250 286 162 628.5 0 162 287 221 628.5 0 221 288 198 628.5 0 198 289 104 628.5 0 104 290 110 628.5 0 110 291 167 628.5 0 167 292 164 628.5 0 164 293 215 628.5 0 215 294 136 628.5 0 136 295 225 628.5 0 225 296 182 628.5 0 182 297 230 628.5 0 230 298 2395 628.5 0 2395 299 3092 628.5 0 3092 300 2017 628.5 0 2017 301 315 628.5 0 315 Finished in 4.35 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 96,198 Reads with adapters: 33,875 (35.2%) Reads that were too short: 66 (0.1%) Reads written (passing filters): 33,809 (35.1%) Total basepairs processed: 25,473,254 bp Total written (filtered): 9,282,962 bp (36.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33875 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 9 16 220 0.0 3 0 214 5 1 17 32256 0.0 3 31603 627 26 18 63 0.0 3 9 48 6 19 1103 0.0 3 1076 15 12 20 41 0.0 3 0 3 0 38 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 29 1 0.0 3 1 33 1 0.0 3 0 0 0 1 39 14 0.0 3 0 0 0 14 42 3 0.0 3 0 0 0 3 44 1 0.0 3 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 38 0.0 3 0 0 0 38 53 4 0.0 3 0 0 0 4 59 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 4 0.0 3 0 0 0 4 79 8 0.0 3 0 0 0 8 80 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 52 0.0 3 0 0 1 51 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.21 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 33,809 Reads with adapters: 1,884 (5.6%) Reads written (passing filters): 33,809 (100.0%) Total basepairs processed: 9,282,962 bp Total written (filtered): 9,277,232 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1884 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.3% C: 6.9% G: 17.1% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1806 528.3 0 1806 4 78 132.1 0 78