This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.93 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 136,499 (5.3%) Reads that were too short: 51,751 (2.0%) Reads written (passing filters): 84,748 (3.3%) Total basepairs processed: 774,927,510 bp Total written (filtered): 22,752,560 bp (2.9%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 136499 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 933 628.5 0 933 7 36293 628.5 0 36293 8 729 628.5 0 729 9 29974 628.5 0 29974 10 481 628.5 0 481 11 32 628.5 0 32 12 48 628.5 0 48 13 43 628.5 0 43 14 20 628.5 0 20 15 21 628.5 0 21 16 30 628.5 0 30 17 16 628.5 0 16 18 14 628.5 0 14 19 15 628.5 0 15 20 28 628.5 0 28 21 49 628.5 0 49 22 20 628.5 0 20 23 30 628.5 0 30 24 18 628.5 0 18 25 27 628.5 0 27 26 33 628.5 0 33 27 11 628.5 0 11 28 29 628.5 0 29 29 35 628.5 0 35 30 30 628.5 0 30 31 16 628.5 0 16 32 29 628.5 0 29 33 32 628.5 0 32 34 25 628.5 0 25 35 18 628.5 0 18 36 15 628.5 0 15 37 29 628.5 0 29 38 23 628.5 0 23 39 25 628.5 0 25 40 37 628.5 0 37 41 28 628.5 0 28 42 29 628.5 0 29 43 27 628.5 0 27 44 22 628.5 0 22 45 22 628.5 0 22 46 23 628.5 0 23 47 27 628.5 0 27 48 21 628.5 0 21 49 34 628.5 0 34 50 18 628.5 0 18 51 17 628.5 0 17 52 26 628.5 0 26 53 40 628.5 0 40 54 39 628.5 0 39 55 104 628.5 0 104 56 71 628.5 0 71 57 56 628.5 0 56 58 18 628.5 0 18 59 60 628.5 0 60 60 42 628.5 0 42 61 76 628.5 0 76 62 34 628.5 0 34 63 37 628.5 0 37 64 42 628.5 0 42 65 47 628.5 0 47 66 17 628.5 0 17 67 35 628.5 0 35 68 27 628.5 0 27 69 54 628.5 0 54 70 183 628.5 0 183 71 76 628.5 0 76 72 46 628.5 0 46 73 37 628.5 0 37 74 104 628.5 0 104 75 103 628.5 0 103 76 157 628.5 0 157 77 44 628.5 0 44 78 45 628.5 0 45 79 186 628.5 0 186 80 63 628.5 0 63 81 254 628.5 0 254 82 375 628.5 0 375 83 305 628.5 0 305 84 573 628.5 0 573 85 119 628.5 0 119 86 122 628.5 0 122 87 31 628.5 0 31 88 37 628.5 0 37 89 66 628.5 0 66 90 36 628.5 0 36 91 20 628.5 0 20 92 33 628.5 0 33 93 26 628.5 0 26 94 13 628.5 0 13 95 33 628.5 0 33 96 38 628.5 0 38 97 32 628.5 0 32 98 27 628.5 0 27 99 29 628.5 0 29 100 23 628.5 0 23 101 18 628.5 0 18 102 32 628.5 0 32 103 40 628.5 0 40 104 65 628.5 0 65 105 29 628.5 0 29 106 35 628.5 0 35 107 31 628.5 0 31 108 29 628.5 0 29 109 19 628.5 0 19 110 41 628.5 0 41 111 41 628.5 0 41 112 45 628.5 0 45 113 68 628.5 0 68 114 55 628.5 0 55 115 41 628.5 0 41 116 22 628.5 0 22 117 35 628.5 0 35 118 45 628.5 0 45 119 39 628.5 0 39 120 44 628.5 0 44 121 158 628.5 0 158 122 277 628.5 0 277 123 372 628.5 0 372 124 49 628.5 0 49 125 112 628.5 0 112 126 73 628.5 0 73 127 88 628.5 0 88 128 70 628.5 0 70 129 60 628.5 0 60 130 40 628.5 0 40 131 34 628.5 0 34 132 33 628.5 0 33 133 68 628.5 0 68 134 49 628.5 0 49 135 43 628.5 0 43 136 21 628.5 0 21 137 23 628.5 0 23 138 64 628.5 0 64 139 50 628.5 0 50 140 54 628.5 0 54 141 34 628.5 0 34 142 20 628.5 0 20 143 36 628.5 0 36 144 33 628.5 0 33 145 40 628.5 0 40 146 27 628.5 0 27 147 52 628.5 0 52 148 51 628.5 0 51 149 25 628.5 0 25 150 35 628.5 0 35 151 43 628.5 0 43 152 65 628.5 0 65 153 93 628.5 0 93 154 62 628.5 0 62 155 35 628.5 0 35 156 35 628.5 0 35 157 51 628.5 0 51 158 44 628.5 0 44 159 41 628.5 0 41 160 37 628.5 0 37 161 38 628.5 0 38 162 48 628.5 0 48 163 76 628.5 0 76 164 47 628.5 0 47 165 75 628.5 0 75 166 91 628.5 0 91 167 699 628.5 0 699 168 370 628.5 0 370 169 785 628.5 0 785 170 132 628.5 0 132 171 281 628.5 0 281 172 190 628.5 0 190 173 353 628.5 0 353 174 82 628.5 0 82 175 49 628.5 0 49 176 43 628.5 0 43 177 40 628.5 0 40 178 44 628.5 0 44 179 40 628.5 0 40 180 184 628.5 0 184 181 198 628.5 0 198 182 363 628.5 0 363 183 98 628.5 0 98 184 100 628.5 0 100 185 96 628.5 0 96 186 98 628.5 0 98 187 85 628.5 0 85 188 109 628.5 0 109 189 94 628.5 0 94 190 85 628.5 0 85 191 93 628.5 0 93 192 59 628.5 0 59 193 71 628.5 0 71 194 88 628.5 0 88 195 71 628.5 0 71 196 84 628.5 0 84 197 94 628.5 0 94 198 131 628.5 0 131 199 215 628.5 0 215 200 1280 628.5 0 1280 201 661 628.5 0 661 202 607 628.5 0 607 203 118 628.5 0 118 204 65 628.5 0 65 205 120 628.5 0 120 206 157 628.5 0 157 207 129 628.5 0 129 208 124 628.5 0 124 209 51 628.5 0 51 210 58 628.5 0 58 211 126 628.5 0 126 212 63 628.5 0 63 213 94 628.5 0 94 214 166 628.5 0 166 215 181 628.5 0 181 216 217 628.5 0 217 217 132 628.5 0 132 218 130 628.5 0 130 219 174 628.5 0 174 220 176 628.5 0 176 221 144 628.5 0 144 222 119 628.5 0 119 223 189 628.5 0 189 224 263 628.5 0 263 225 338 628.5 0 338 226 694 628.5 0 694 227 1233 628.5 0 1233 228 12491 628.5 0 12491 229 8739 628.5 0 8739 230 8472 628.5 0 8472 231 250 628.5 0 250 232 124 628.5 0 124 233 62 628.5 0 62 234 52 628.5 0 52 235 45 628.5 0 45 236 87 628.5 0 87 237 83 628.5 0 83 238 101 628.5 0 101 239 119 628.5 0 119 240 99 628.5 0 99 241 78 628.5 0 78 242 80 628.5 0 80 243 122 628.5 0 122 244 104 628.5 0 104 245 135 628.5 0 135 246 221 628.5 0 221 247 296 628.5 0 296 248 388 628.5 0 388 249 335 628.5 0 335 250 200 628.5 0 200 251 93 628.5 0 93 252 78 628.5 0 78 253 71 628.5 0 71 254 103 628.5 0 103 255 112 628.5 0 112 256 348 628.5 0 348 257 411 628.5 0 411 258 408 628.5 0 408 259 123 628.5 0 123 260 139 628.5 0 139 261 384 628.5 0 384 262 641 628.5 0 641 263 998 628.5 0 998 264 280 628.5 0 280 265 365 628.5 0 365 266 148 628.5 0 148 267 226 628.5 0 226 268 787 628.5 0 787 269 765 628.5 0 765 270 616 628.5 0 616 271 208 628.5 0 208 272 110 628.5 0 110 273 86 628.5 0 86 274 126 628.5 0 126 275 152 628.5 0 152 276 138 628.5 0 138 277 149 628.5 0 149 278 187 628.5 0 187 279 95 628.5 0 95 280 122 628.5 0 122 281 206 628.5 0 206 282 281 628.5 0 281 283 345 628.5 0 345 284 128 628.5 0 128 285 78 628.5 0 78 286 107 628.5 0 107 287 159 628.5 0 159 288 232 628.5 0 232 289 136 628.5 0 136 290 89 628.5 0 89 291 89 628.5 0 89 292 351 628.5 0 351 293 436 628.5 0 436 294 399 628.5 0 399 295 143 628.5 0 143 296 99 628.5 0 99 297 86 628.5 0 86 298 100 628.5 0 100 299 131 628.5 0 131 300 417 628.5 0 417 301 719 628.5 0 719 Finished in 4.39 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 84,748 Reads with adapters: 32,106 (37.9%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 32,068 (37.8%) Total basepairs processed: 22,752,560 bp Total written (filtered): 8,623,872 bp (37.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32106 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 3 5 18 18 0.0 3 0 0 11 2 5 19 333 0.0 3 0 244 30 38 21 20 30427 0.0 4 27505 2232 481 147 62 21 77 0.0 4 10 59 4 1 3 22 13 0.0 4 0 1 8 3 1 23 235 0.0 4 5 0 0 142 88 24 4 0.0 4 1 0 0 0 3 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 1 61 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 1 112 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 13 0.0 4 0 0 0 0 13 127 914 0.0 4 0 0 0 0 914 128 7 0.0 4 0 0 0 0 7 129 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 175 1 0.0 4 0 0 0 1 179 1 0.0 4 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 284 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.17 s (36 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 32,068 Reads with adapters: 8,343 (26.0%) Reads written (passing filters): 32,068 (100.0%) Total basepairs processed: 8,623,872 bp Total written (filtered): 8,534,053 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8343 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 8.5% G: 87.1% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 22 501.1 0 22 4 37 125.3 0 37 7 1 2.0 0 1 9 2609 0.1 0 266 2343 10 4791 0.0 1 437 4354 11 789 0.0 1 31 758 12 28 0.0 1 2 26 13 24 0.0 1 0 24 14 3 0.0 1 0 3 39 1 0.0 1 1 150 5 0.0 1 1 4 152 1 0.0 1 0 1 178 1 0.0 1 1 208 3 0.0 1 1 2 209 1 0.0 1 1 223 1 0.0 1 1 256 24 0.0 1 23 1 257 1 0.0 1 1 262 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.