This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.09 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 144,357 (5.6%) Reads that were too short: 59,485 (2.3%) Reads written (passing filters): 84,872 (3.3%) Total basepairs processed: 774,927,510 bp Total written (filtered): 23,198,699 bp (3.0%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 144357 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 463 628.5 0 463 7 32665 628.5 0 32665 8 538 628.5 0 538 9 38459 628.5 0 38459 10 67 628.5 0 67 11 15 628.5 0 15 12 27 628.5 0 27 13 5 628.5 0 5 14 13 628.5 0 13 15 16 628.5 0 16 16 14 628.5 0 14 17 14 628.5 0 14 18 15 628.5 0 15 19 14 628.5 0 14 20 7 628.5 0 7 21 15 628.5 0 15 22 16 628.5 0 16 23 9 628.5 0 9 24 7 628.5 0 7 25 11 628.5 0 11 26 24 628.5 0 24 27 14 628.5 0 14 28 17 628.5 0 17 29 13 628.5 0 13 30 22 628.5 0 22 31 12 628.5 0 12 32 17 628.5 0 17 33 28 628.5 0 28 34 23 628.5 0 23 35 15 628.5 0 15 36 24 628.5 0 24 37 33 628.5 0 33 38 12 628.5 0 12 39 17 628.5 0 17 40 19 628.5 0 19 41 16 628.5 0 16 42 14 628.5 0 14 43 14 628.5 0 14 44 20 628.5 0 20 45 10 628.5 0 10 46 17 628.5 0 17 47 19 628.5 0 19 48 16 628.5 0 16 49 18 628.5 0 18 50 12 628.5 0 12 51 9 628.5 0 9 52 14 628.5 0 14 53 12 628.5 0 12 54 11 628.5 0 11 55 22 628.5 0 22 56 23 628.5 0 23 57 18 628.5 0 18 58 12 628.5 0 12 59 20 628.5 0 20 60 30 628.5 0 30 61 30 628.5 0 30 62 22 628.5 0 22 63 23 628.5 0 23 64 17 628.5 0 17 65 22 628.5 0 22 66 18 628.5 0 18 67 33 628.5 0 33 68 20 628.5 0 20 69 33 628.5 0 33 70 149 628.5 0 149 71 51 628.5 0 51 72 34 628.5 0 34 73 28 628.5 0 28 74 86 628.5 0 86 75 121 628.5 0 121 76 125 628.5 0 125 77 37 628.5 0 37 78 42 628.5 0 42 79 232 628.5 0 232 80 63 628.5 0 63 81 150 628.5 0 150 82 355 628.5 0 355 83 154 628.5 0 154 84 529 628.5 0 529 85 31 628.5 0 31 86 40 628.5 0 40 87 24 628.5 0 24 88 25 628.5 0 25 89 20 628.5 0 20 90 18 628.5 0 18 91 28 628.5 0 28 92 31 628.5 0 31 93 29 628.5 0 29 94 26 628.5 0 26 95 31 628.5 0 31 96 28 628.5 0 28 97 27 628.5 0 27 98 26 628.5 0 26 99 32 628.5 0 32 100 22 628.5 0 22 101 34 628.5 0 34 102 31 628.5 0 31 103 30 628.5 0 30 104 27 628.5 0 27 105 37 628.5 0 37 106 31 628.5 0 31 107 27 628.5 0 27 108 25 628.5 0 25 109 21 628.5 0 21 110 29 628.5 0 29 111 38 628.5 0 38 112 32 628.5 0 32 113 38 628.5 0 38 114 38 628.5 0 38 115 36 628.5 0 36 116 25 628.5 0 25 117 27 628.5 0 27 118 30 628.5 0 30 119 39 628.5 0 39 120 26 628.5 0 26 121 44 628.5 0 44 122 65 628.5 0 65 123 143 628.5 0 143 124 49 628.5 0 49 125 99 628.5 0 99 126 47 628.5 0 47 127 37 628.5 0 37 128 51 628.5 0 51 129 39 628.5 0 39 130 35 628.5 0 35 131 32 628.5 0 32 132 32 628.5 0 32 133 38 628.5 0 38 134 40 628.5 0 40 135 30 628.5 0 30 136 42 628.5 0 42 137 37 628.5 0 37 138 64 628.5 0 64 139 45 628.5 0 45 140 46 628.5 0 46 141 32 628.5 0 32 142 26 628.5 0 26 143 38 628.5 0 38 144 41 628.5 0 41 145 51 628.5 0 51 146 54 628.5 0 54 147 48 628.5 0 48 148 60 628.5 0 60 149 54 628.5 0 54 150 65 628.5 0 65 151 52 628.5 0 52 152 56 628.5 0 56 153 44 628.5 0 44 154 55 628.5 0 55 155 55 628.5 0 55 156 60 628.5 0 60 157 69 628.5 0 69 158 57 628.5 0 57 159 50 628.5 0 50 160 67 628.5 0 67 161 61 628.5 0 61 162 74 628.5 0 74 163 82 628.5 0 82 164 72 628.5 0 72 165 92 628.5 0 92 166 118 628.5 0 118 167 731 628.5 0 731 168 242 628.5 0 242 169 687 628.5 0 687 170 87 628.5 0 87 171 200 628.5 0 200 172 128 628.5 0 128 173 199 628.5 0 199 174 79 628.5 0 79 175 76 628.5 0 76 176 89 628.5 0 89 177 69 628.5 0 69 178 81 628.5 0 81 179 100 628.5 0 100 180 115 628.5 0 115 181 112 628.5 0 112 182 172 628.5 0 172 183 100 628.5 0 100 184 98 628.5 0 98 185 100 628.5 0 100 186 127 628.5 0 127 187 100 628.5 0 100 188 99 628.5 0 99 189 131 628.5 0 131 190 89 628.5 0 89 191 135 628.5 0 135 192 100 628.5 0 100 193 96 628.5 0 96 194 104 628.5 0 104 195 110 628.5 0 110 196 109 628.5 0 109 197 100 628.5 0 100 198 124 628.5 0 124 199 137 628.5 0 137 200 496 628.5 0 496 201 362 628.5 0 362 202 276 628.5 0 276 203 138 628.5 0 138 204 102 628.5 0 102 205 127 628.5 0 127 206 112 628.5 0 112 207 133 628.5 0 133 208 115 628.5 0 115 209 97 628.5 0 97 210 124 628.5 0 124 211 210 628.5 0 210 212 116 628.5 0 116 213 152 628.5 0 152 214 143 628.5 0 143 215 162 628.5 0 162 216 200 628.5 0 200 217 165 628.5 0 165 218 149 628.5 0 149 219 144 628.5 0 144 220 150 628.5 0 150 221 178 628.5 0 178 222 188 628.5 0 188 223 253 628.5 0 253 224 318 628.5 0 318 225 380 628.5 0 380 226 773 628.5 0 773 227 1467 628.5 0 1467 228 16340 628.5 0 16340 229 11304 628.5 0 11304 230 10598 628.5 0 10598 231 216 628.5 0 216 232 114 628.5 0 114 233 122 628.5 0 122 234 117 628.5 0 117 235 112 628.5 0 112 236 127 628.5 0 127 237 131 628.5 0 131 238 131 628.5 0 131 239 131 628.5 0 131 240 132 628.5 0 132 241 129 628.5 0 129 242 131 628.5 0 131 243 128 628.5 0 128 244 112 628.5 0 112 245 129 628.5 0 129 246 158 628.5 0 158 247 174 628.5 0 174 248 175 628.5 0 175 249 181 628.5 0 181 250 146 628.5 0 146 251 136 628.5 0 136 252 127 628.5 0 127 253 117 628.5 0 117 254 175 628.5 0 175 255 155 628.5 0 155 256 308 628.5 0 308 257 315 628.5 0 315 258 288 628.5 0 288 259 168 628.5 0 168 260 134 628.5 0 134 261 288 628.5 0 288 262 441 628.5 0 441 263 655 628.5 0 655 264 266 628.5 0 266 265 290 628.5 0 290 266 160 628.5 0 160 267 200 628.5 0 200 268 826 628.5 0 826 269 777 628.5 0 777 270 633 628.5 0 633 271 218 628.5 0 218 272 188 628.5 0 188 273 138 628.5 0 138 274 152 628.5 0 152 275 138 628.5 0 138 276 204 628.5 0 204 277 268 628.5 0 268 278 299 628.5 0 299 279 150 628.5 0 150 280 178 628.5 0 178 281 160 628.5 0 160 282 206 628.5 0 206 283 296 628.5 0 296 284 139 628.5 0 139 285 133 628.5 0 133 286 133 628.5 0 133 287 171 628.5 0 171 288 221 628.5 0 221 289 160 628.5 0 160 290 150 628.5 0 150 291 145 628.5 0 145 292 164 628.5 0 164 293 194 628.5 0 194 294 186 628.5 0 186 295 121 628.5 0 121 296 161 628.5 0 161 297 138 628.5 0 138 298 136 628.5 0 136 299 157 628.5 0 157 300 239 628.5 0 239 301 273 628.5 0 273 Finished in 3.94 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 84,872 Reads with adapters: 32,513 (38.3%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 32,492 (38.3%) Total basepairs processed: 23,198,699 bp Total written (filtered): 8,997,577 bp (38.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32513 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 9 16 238 0.0 3 0 233 5 17 32133 0.0 3 31502 615 15 1 18 51 0.0 3 5 44 2 19 16 0.0 3 1 0 14 1 20 24 0.0 3 1 0 0 23 21 1 0.0 3 1 24 2 0.0 3 0 0 0 2 31 6 0.0 3 0 0 0 6 46 4 0.0 3 1 0 0 3 60 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 1 164 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.14 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 32,492 Reads with adapters: 59 (0.2%) Reads written (passing filters): 32,492 (100.0%) Total basepairs processed: 8,997,577 bp Total written (filtered): 8,997,395 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 59 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.8% C: 39.0% G: 15.3% T: 39.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 55 507.7 0 55 4 3 126.9 0 3 5 1 31.7 0 1