This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.03 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 1,132,152 (44.0%) Reads that were too short: 34,095 (1.3%) Reads written (passing filters): 1,098,057 (42.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 299,518,735 bp (38.7%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1132152 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2920 628.5 0 2920 7 80859 628.5 0 80859 8 5959 628.5 0 5959 9 81046 628.5 0 81046 10 613 628.5 0 613 11 469 628.5 0 469 12 7164 628.5 0 7164 13 2148 628.5 0 2148 14 419 628.5 0 419 15 1047 628.5 0 1047 16 456 628.5 0 456 17 643 628.5 0 643 18 1593 628.5 0 1593 19 778 628.5 0 778 20 834 628.5 0 834 21 332 628.5 0 332 22 597 628.5 0 597 23 1958 628.5 0 1958 24 2175 628.5 0 2175 25 4045 628.5 0 4045 26 5737 628.5 0 5737 27 6104 628.5 0 6104 28 15237 628.5 0 15237 29 23677 628.5 0 23677 30 304141 628.5 0 304141 31 172286 628.5 0 172286 32 356403 628.5 0 356403 33 1447 628.5 0 1447 34 147 628.5 0 147 35 348 628.5 0 348 36 554 628.5 0 554 37 528 628.5 0 528 38 36 628.5 0 36 39 32 628.5 0 32 40 28 628.5 0 28 41 63 628.5 0 63 42 36 628.5 0 36 43 43 628.5 0 43 44 112 628.5 0 112 45 33 628.5 0 33 46 43 628.5 0 43 47 49 628.5 0 49 48 31 628.5 0 31 49 37 628.5 0 37 50 35 628.5 0 35 51 40 628.5 0 40 52 38 628.5 0 38 53 54 628.5 0 54 54 404 628.5 0 404 55 658 628.5 0 658 56 760 628.5 0 760 57 42 628.5 0 42 58 32 628.5 0 32 59 15 628.5 0 15 60 23 628.5 0 23 61 25 628.5 0 25 62 27 628.5 0 27 63 16 628.5 0 16 64 27 628.5 0 27 65 28 628.5 0 28 66 24 628.5 0 24 67 17 628.5 0 17 68 40 628.5 0 40 69 71 628.5 0 71 70 110 628.5 0 110 71 66 628.5 0 66 72 30 628.5 0 30 73 33 628.5 0 33 74 26 628.5 0 26 75 29 628.5 0 29 76 24 628.5 0 24 77 27 628.5 0 27 78 19 628.5 0 19 79 14 628.5 0 14 80 28 628.5 0 28 81 35 628.5 0 35 82 99 628.5 0 99 83 34 628.5 0 34 84 31 628.5 0 31 85 29 628.5 0 29 86 32 628.5 0 32 87 52 628.5 0 52 88 48 628.5 0 48 89 123 628.5 0 123 90 48 628.5 0 48 91 38 628.5 0 38 92 24 628.5 0 24 93 15 628.5 0 15 94 22 628.5 0 22 95 33 628.5 0 33 96 44 628.5 0 44 97 31 628.5 0 31 98 31 628.5 0 31 99 32 628.5 0 32 100 39 628.5 0 39 101 39 628.5 0 39 102 184 628.5 0 184 103 317 628.5 0 317 104 1016 628.5 0 1016 105 758 628.5 0 758 106 443 628.5 0 443 107 72 628.5 0 72 108 26 628.5 0 26 109 29 628.5 0 29 110 21 628.5 0 21 111 44 628.5 0 44 112 50 628.5 0 50 113 62 628.5 0 62 114 44 628.5 0 44 115 24 628.5 0 24 116 28 628.5 0 28 117 23 628.5 0 23 118 40 628.5 0 40 119 38 628.5 0 38 120 32 628.5 0 32 121 23 628.5 0 23 122 32 628.5 0 32 123 64 628.5 0 64 124 185 628.5 0 185 125 193 628.5 0 193 126 211 628.5 0 211 127 50 628.5 0 50 128 35 628.5 0 35 129 26 628.5 0 26 130 25 628.5 0 25 131 33 628.5 0 33 132 44 628.5 0 44 133 64 628.5 0 64 134 56 628.5 0 56 135 50 628.5 0 50 136 55 628.5 0 55 137 430 628.5 0 430 138 1024 628.5 0 1024 139 1787 628.5 0 1787 140 83 628.5 0 83 141 202 628.5 0 202 142 185 628.5 0 185 143 230 628.5 0 230 144 49 628.5 0 49 145 26 628.5 0 26 146 43 628.5 0 43 147 67 628.5 0 67 148 71 628.5 0 71 149 191 628.5 0 191 150 59 628.5 0 59 151 98 628.5 0 98 152 34 628.5 0 34 153 25 628.5 0 25 154 39 628.5 0 39 155 33 628.5 0 33 156 34 628.5 0 34 157 24 628.5 0 24 158 43 628.5 0 43 159 84 628.5 0 84 160 37 628.5 0 37 161 85 628.5 0 85 162 38 628.5 0 38 163 61 628.5 0 61 164 52 628.5 0 52 165 28 628.5 0 28 166 42 628.5 0 42 167 65 628.5 0 65 168 72 628.5 0 72 169 33 628.5 0 33 170 40 628.5 0 40 171 54 628.5 0 54 172 24 628.5 0 24 173 27 628.5 0 27 174 30 628.5 0 30 175 23 628.5 0 23 176 26 628.5 0 26 177 46 628.5 0 46 178 36 628.5 0 36 179 35 628.5 0 35 180 34 628.5 0 34 181 48 628.5 0 48 182 90 628.5 0 90 183 90 628.5 0 90 184 108 628.5 0 108 185 75 628.5 0 75 186 71 628.5 0 71 187 36 628.5 0 36 188 48 628.5 0 48 189 41 628.5 0 41 190 30 628.5 0 30 191 26 628.5 0 26 192 28 628.5 0 28 193 35 628.5 0 35 194 32 628.5 0 32 195 37 628.5 0 37 196 34 628.5 0 34 197 26 628.5 0 26 198 39 628.5 0 39 199 54 628.5 0 54 200 43 628.5 0 43 201 52 628.5 0 52 202 32 628.5 0 32 203 29 628.5 0 29 204 82 628.5 0 82 205 178 628.5 0 178 206 315 628.5 0 315 207 209 628.5 0 209 208 39 628.5 0 39 209 42 628.5 0 42 210 47 628.5 0 47 211 55 628.5 0 55 212 90 628.5 0 90 213 94 628.5 0 94 214 52 628.5 0 52 215 56 628.5 0 56 216 55 628.5 0 55 217 32 628.5 0 32 218 46 628.5 0 46 219 68 628.5 0 68 220 72 628.5 0 72 221 53 628.5 0 53 222 37 628.5 0 37 223 24 628.5 0 24 224 50 628.5 0 50 225 56 628.5 0 56 226 114 628.5 0 114 227 125 628.5 0 125 228 131 628.5 0 131 229 49 628.5 0 49 230 49 628.5 0 49 231 112 628.5 0 112 232 165 628.5 0 165 233 129 628.5 0 129 234 59 628.5 0 59 235 31 628.5 0 31 236 51 628.5 0 51 237 45 628.5 0 45 238 38 628.5 0 38 239 49 628.5 0 49 240 40 628.5 0 40 241 57 628.5 0 57 242 46 628.5 0 46 243 50 628.5 0 50 244 46 628.5 0 46 245 72 628.5 0 72 246 91 628.5 0 91 247 141 628.5 0 141 248 76 628.5 0 76 249 81 628.5 0 81 250 124 628.5 0 124 251 70 628.5 0 70 252 74 628.5 0 74 253 115 628.5 0 115 254 653 628.5 0 653 255 1168 628.5 0 1168 256 816 628.5 0 816 257 599 628.5 0 599 258 442 628.5 0 442 259 431 628.5 0 431 260 5052 628.5 0 5052 261 8621 628.5 0 8621 262 4472 628.5 0 4472 263 92 628.5 0 92 264 56 628.5 0 56 265 65 628.5 0 65 266 66 628.5 0 66 267 119 628.5 0 119 268 148 628.5 0 148 269 164 628.5 0 164 270 104 628.5 0 104 271 54 628.5 0 54 272 241 628.5 0 241 273 270 628.5 0 270 274 329 628.5 0 329 275 838 628.5 0 838 276 764 628.5 0 764 277 872 628.5 0 872 278 511 628.5 0 511 279 417 628.5 0 417 280 339 628.5 0 339 281 450 628.5 0 450 282 530 628.5 0 530 283 564 628.5 0 564 284 47 628.5 0 47 285 29 628.5 0 29 286 35 628.5 0 35 287 53 628.5 0 53 288 37 628.5 0 37 289 59 628.5 0 59 290 55 628.5 0 55 291 41 628.5 0 41 292 44 628.5 0 44 293 51 628.5 0 51 294 80 628.5 0 80 295 63 628.5 0 63 296 60 628.5 0 60 297 15 628.5 0 15 298 32 628.5 0 32 299 26 628.5 0 26 300 40 628.5 0 40 301 38 628.5 0 38 Finished in 51.37 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 1,098,057 Reads with adapters: 82,688 (7.5%) Reads that were too short: 95 (0.0%) Reads written (passing filters): 82,593 (7.5%) Total basepairs processed: 299,518,735 bp Total written (filtered): 22,480,510 bp (7.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 82688 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 41 0.0 3 0 0 27 10 4 19 911 0.0 3 0 700 121 69 21 20 79942 0.0 4 74408 4265 910 258 101 21 165 0.0 4 24 121 12 4 4 22 17 0.0 4 0 0 10 3 4 23 185 0.0 4 0 0 1 135 49 24 2 0.0 4 0 0 0 0 2 27 2 0.0 4 1 0 0 1 28 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 2 0.0 4 2 37 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 1 79 2 0.0 4 1 1 80 31 0.0 4 0 0 0 0 31 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 92 5 0.0 4 0 0 1 0 4 96 1 0.0 4 1 101 1 0.0 4 0 0 1 103 1 0.0 4 0 1 104 1 0.0 4 0 0 0 0 1 113 3 0.0 4 1 0 1 0 1 114 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 1 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 1 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 213 0.0 4 0 0 0 0 213 125 1 0.0 4 0 0 0 0 1 126 15 0.0 4 0 0 0 0 15 127 825 0.0 4 0 0 0 1 824 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 130 15 0.0 4 0 0 0 1 14 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 14 0.0 4 0 0 1 0 13 147 1 0.0 4 0 0 0 0 1 148 85 0.0 4 0 0 0 1 84 149 55 0.0 4 0 0 1 0 54 150 9 0.0 4 0 0 0 0 9 153 6 0.0 4 0 0 0 0 6 154 2 0.0 4 0 0 0 0 2 159 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 179 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 182 6 0.0 4 0 0 0 0 6 184 1 0.0 4 0 0 0 0 1 188 3 0.0 4 0 0 0 0 3 193 2 0.0 4 0 0 0 0 2 194 2 0.0 4 0 0 0 0 2 195 2 0.0 4 0 0 0 0 2 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 1 209 3 0.0 4 0 0 0 0 3 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 3 0.0 4 0 0 0 0 3 214 4 0.0 4 0 0 0 2 2 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 3 0.0 4 0 0 0 0 3 222 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 228 3 0.0 4 0 0 0 0 3 229 4 0.0 4 0 0 1 0 3 233 3 0.0 4 0 0 0 0 3 234 3 0.0 4 0 0 0 0 3 235 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 1 238 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.86 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 82,593 Reads with adapters: 4,717 (5.7%) Reads written (passing filters): 82,593 (100.0%) Total basepairs processed: 22,480,510 bp Total written (filtered): 22,351,600 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4717 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.7% C: 20.7% G: 65.8% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 599 1290.5 0 599 4 67 322.6 0 67 5 8 80.7 0 8 6 2 20.2 0 2 9 31 0.3 0 5 26 10 63 0.1 1 2 61 11 1796 0.0 1 90 1706 12 1769 0.0 1 50 1719 13 43 0.0 1 0 43 14 1 0.0 1 0 1 15 6 0.0 1 0 6 16 2 0.0 1 0 2 149 1 0.0 1 1 152 25 0.0 1 17 8 171 1 0.0 1 0 1 179 2 0.0 1 2 191 1 0.0 1 1 210 19 0.0 1 13 6 230 1 0.0 1 1 259 1 0.0 1 1 267 1 0.0 1 1 268 1 0.0 1 1 269 268 0.0 1 251 17 270 9 0.0 1 7 2