This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.83 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 119,589 (4.6%) Reads that were too short: 29,017 (1.1%) Reads written (passing filters): 90,572 (3.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 21,939,142 bp (2.8%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 119589 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1417 628.5 0 1417 7 566 628.5 0 566 8 21992 628.5 0 21992 9 20385 628.5 0 20385 10 76 628.5 0 76 11 42 628.5 0 42 12 57 628.5 0 57 13 50 628.5 0 50 14 52 628.5 0 52 15 49 628.5 0 49 16 43 628.5 0 43 17 51 628.5 0 51 18 64 628.5 0 64 19 49 628.5 0 49 20 34 628.5 0 34 21 39 628.5 0 39 22 49 628.5 0 49 23 51 628.5 0 51 24 84 628.5 0 84 25 83 628.5 0 83 26 39 628.5 0 39 27 41 628.5 0 41 28 30 628.5 0 30 29 32 628.5 0 32 30 52 628.5 0 52 31 45 628.5 0 45 32 57 628.5 0 57 33 49 628.5 0 49 34 47 628.5 0 47 35 91 628.5 0 91 36 37 628.5 0 37 37 39 628.5 0 39 38 39 628.5 0 39 39 41 628.5 0 41 40 42 628.5 0 42 41 55 628.5 0 55 42 71 628.5 0 71 43 53 628.5 0 53 44 41 628.5 0 41 45 26 628.5 0 26 46 69 628.5 0 69 47 50 628.5 0 50 48 33 628.5 0 33 49 46 628.5 0 46 50 39 628.5 0 39 51 79 628.5 0 79 52 118 628.5 0 118 53 88 628.5 0 88 54 469 628.5 0 469 55 80 628.5 0 80 56 52 628.5 0 52 57 102 628.5 0 102 58 71 628.5 0 71 59 57 628.5 0 57 60 59 628.5 0 59 61 67 628.5 0 67 62 103 628.5 0 103 63 111 628.5 0 111 64 108 628.5 0 108 65 182 628.5 0 182 66 163 628.5 0 163 67 425 628.5 0 425 68 595 628.5 0 595 69 6547 628.5 0 6547 70 2694 628.5 0 2694 71 7161 628.5 0 7161 72 69 628.5 0 69 73 56 628.5 0 56 74 44 628.5 0 44 75 74 628.5 0 74 76 66 628.5 0 66 77 55 628.5 0 55 78 58 628.5 0 58 79 56 628.5 0 56 80 222 628.5 0 222 81 118 628.5 0 118 82 187 628.5 0 187 83 63 628.5 0 63 84 62 628.5 0 62 85 49 628.5 0 49 86 67 628.5 0 67 87 117 628.5 0 117 88 83 628.5 0 83 89 74 628.5 0 74 90 78 628.5 0 78 91 70 628.5 0 70 92 60 628.5 0 60 93 84 628.5 0 84 94 110 628.5 0 110 95 89 628.5 0 89 96 88 628.5 0 88 97 104 628.5 0 104 98 76 628.5 0 76 99 111 628.5 0 111 100 914 628.5 0 914 101 1752 628.5 0 1752 102 1891 628.5 0 1891 103 77 628.5 0 77 104 59 628.5 0 59 105 46 628.5 0 46 106 53 628.5 0 53 107 63 628.5 0 63 108 31 628.5 0 31 109 59 628.5 0 59 110 89 628.5 0 89 111 83 628.5 0 83 112 60 628.5 0 60 113 99 628.5 0 99 114 104 628.5 0 104 115 80 628.5 0 80 116 67 628.5 0 67 117 63 628.5 0 63 118 98 628.5 0 98 119 85 628.5 0 85 120 78 628.5 0 78 121 71 628.5 0 71 122 101 628.5 0 101 123 97 628.5 0 97 124 113 628.5 0 113 125 76 628.5 0 76 126 76 628.5 0 76 127 68 628.5 0 68 128 77 628.5 0 77 129 55 628.5 0 55 130 100 628.5 0 100 131 101 628.5 0 101 132 416 628.5 0 416 133 423 628.5 0 423 134 395 628.5 0 395 135 99 628.5 0 99 136 499 628.5 0 499 137 742 628.5 0 742 138 388 628.5 0 388 139 152 628.5 0 152 140 86 628.5 0 86 141 72 628.5 0 72 142 76 628.5 0 76 143 72 628.5 0 72 144 94 628.5 0 94 145 98 628.5 0 98 146 99 628.5 0 99 147 66 628.5 0 66 148 78 628.5 0 78 149 68 628.5 0 68 150 96 628.5 0 96 151 101 628.5 0 101 152 114 628.5 0 114 153 140 628.5 0 140 154 116 628.5 0 116 155 85 628.5 0 85 156 80 628.5 0 80 157 174 628.5 0 174 158 225 628.5 0 225 159 325 628.5 0 325 160 138 628.5 0 138 161 93 628.5 0 93 162 115 628.5 0 115 163 145 628.5 0 145 164 152 628.5 0 152 165 99 628.5 0 99 166 125 628.5 0 125 167 174 628.5 0 174 168 153 628.5 0 153 169 213 628.5 0 213 170 185 628.5 0 185 171 114 628.5 0 114 172 132 628.5 0 132 173 98 628.5 0 98 174 111 628.5 0 111 175 104 628.5 0 104 176 263 628.5 0 263 177 403 628.5 0 403 178 712 628.5 0 712 179 794 628.5 0 794 180 258 628.5 0 258 181 219 628.5 0 219 182 660 628.5 0 660 183 714 628.5 0 714 184 1231 628.5 0 1231 185 223 628.5 0 223 186 144 628.5 0 144 187 145 628.5 0 145 188 117 628.5 0 117 189 198 628.5 0 198 190 169 628.5 0 169 191 123 628.5 0 123 192 152 628.5 0 152 193 183 628.5 0 183 194 127 628.5 0 127 195 99 628.5 0 99 196 143 628.5 0 143 197 214 628.5 0 214 198 306 628.5 0 306 199 256 628.5 0 256 200 150 628.5 0 150 201 135 628.5 0 135 202 929 628.5 0 929 203 1262 628.5 0 1262 204 1646 628.5 0 1646 205 1641 628.5 0 1641 206 87 628.5 0 87 207 88 628.5 0 88 208 83 628.5 0 83 209 90 628.5 0 90 210 124 628.5 0 124 211 220 628.5 0 220 212 182 628.5 0 182 213 158 628.5 0 158 214 91 628.5 0 91 215 99 628.5 0 99 216 111 628.5 0 111 217 155 628.5 0 155 218 148 628.5 0 148 219 137 628.5 0 137 220 96 628.5 0 96 221 129 628.5 0 129 222 409 628.5 0 409 223 892 628.5 0 892 224 894 628.5 0 894 225 551 628.5 0 551 226 223 628.5 0 223 227 161 628.5 0 161 228 140 628.5 0 140 229 177 628.5 0 177 230 95 628.5 0 95 231 107 628.5 0 107 232 95 628.5 0 95 233 87 628.5 0 87 234 113 628.5 0 113 235 96 628.5 0 96 236 73 628.5 0 73 237 80 628.5 0 80 238 125 628.5 0 125 239 94 628.5 0 94 240 138 628.5 0 138 241 117 628.5 0 117 242 451 628.5 0 451 243 715 628.5 0 715 244 834 628.5 0 834 245 795 628.5 0 795 246 134 628.5 0 134 247 87 628.5 0 87 248 83 628.5 0 83 249 84 628.5 0 84 250 101 628.5 0 101 251 170 628.5 0 170 252 196 628.5 0 196 253 186 628.5 0 186 254 109 628.5 0 109 255 704 628.5 0 704 256 272 628.5 0 272 257 412 628.5 0 412 258 194 628.5 0 194 259 165 628.5 0 165 260 117 628.5 0 117 261 101 628.5 0 101 262 159 628.5 0 159 263 194 628.5 0 194 264 256 628.5 0 256 265 601 628.5 0 601 266 336 628.5 0 336 267 294 628.5 0 294 268 135 628.5 0 135 269 422 628.5 0 422 270 330 628.5 0 330 271 654 628.5 0 654 272 798 628.5 0 798 273 473 628.5 0 473 274 202 628.5 0 202 275 226 628.5 0 226 276 229 628.5 0 229 277 269 628.5 0 269 278 408 628.5 0 408 279 455 628.5 0 455 280 124 628.5 0 124 281 146 628.5 0 146 282 97 628.5 0 97 283 184 628.5 0 184 284 182 628.5 0 182 285 232 628.5 0 232 286 207 628.5 0 207 287 153 628.5 0 153 288 151 628.5 0 151 289 125 628.5 0 125 290 122 628.5 0 122 291 299 628.5 0 299 292 491 628.5 0 491 293 462 628.5 0 462 294 411 628.5 0 411 295 112 628.5 0 112 296 97 628.5 0 97 297 115 628.5 0 115 298 190 628.5 0 190 299 179 628.5 0 179 300 264 628.5 0 264 301 180 628.5 0 180 Finished in 4.30 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 90,572 Reads with adapters: 21,913 (24.2%) Reads that were too short: 56 (0.1%) Reads written (passing filters): 21,857 (24.1%) Total basepairs processed: 21,939,142 bp Total written (filtered): 5,879,978 bp (26.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21913 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 5 4 18 25 0.0 3 0 0 14 8 3 19 398 0.0 3 0 321 29 35 13 20 20595 0.0 4 18662 1489 328 89 27 21 48 0.0 4 8 30 4 1 5 22 17 0.0 4 3 3 8 0 3 23 144 0.0 4 3 3 2 86 50 24 4 0.0 4 0 0 0 0 4 34 10 0.0 4 0 0 0 0 10 36 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 40 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 52 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 3 0.0 4 0 0 0 0 3 61 2 0.0 4 0 0 0 0 2 62 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 1 65 2 0.0 4 0 0 0 1 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 3 0.0 4 0 0 1 0 2 80 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 462 0.0 4 0 0 0 0 462 128 6 0.0 4 0 0 0 0 6 129 3 0.0 4 0 0 0 0 3 130 113 0.0 4 0 0 0 1 112 131 1 0.0 4 0 0 0 0 1 133 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 192 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 236 2 0.0 4 0 0 0 0 2 256 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.79 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 21,857 Reads with adapters: 4,948 (22.6%) Reads written (passing filters): 21,857 (100.0%) Total basepairs processed: 5,879,978 bp Total written (filtered): 5,817,341 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4948 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 7.8% G: 86.1% T: 3.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 341.5 0 9 4 1 85.4 0 1 6 2 5.3 0 2 7 1 1.3 0 1 8 1 0.3 0 1 9 658 0.1 0 97 561 10 3746 0.0 1 313 3433 11 432 0.0 1 25 407 12 15 0.0 1 0 15 13 17 0.0 1 0 17 14 1 0.0 1 0 1 24 1 0.0 1 0 1 150 2 0.0 1 1 1 168 1 0.0 1 1 169 1 0.0 1 1 208 1 0.0 1 0 1 215 1 0.0 1 1 223 51 0.0 1 44 7 224 4 0.0 1 4 225 1 0.0 1 1 226 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.