This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.28 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 98,932 (3.8%) Reads that were too short: 12,256 (0.5%) Reads written (passing filters): 86,676 (3.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 22,108,935 bp (2.9%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 98932 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1421 628.5 0 1421 7 499 628.5 0 499 8 21818 628.5 0 21818 9 23735 628.5 0 23735 10 98 628.5 0 98 11 48 628.5 0 48 12 66 628.5 0 66 13 67 628.5 0 67 14 53 628.5 0 53 15 56 628.5 0 56 16 56 628.5 0 56 17 66 628.5 0 66 18 67 628.5 0 67 19 75 628.5 0 75 20 74 628.5 0 74 21 40 628.5 0 40 22 49 628.5 0 49 23 47 628.5 0 47 24 41 628.5 0 41 25 58 628.5 0 58 26 43 628.5 0 43 27 57 628.5 0 57 28 59 628.5 0 59 29 38 628.5 0 38 30 63 628.5 0 63 31 67 628.5 0 67 32 57 628.5 0 57 33 63 628.5 0 63 34 63 628.5 0 63 35 71 628.5 0 71 36 68 628.5 0 68 37 53 628.5 0 53 38 41 628.5 0 41 39 65 628.5 0 65 40 54 628.5 0 54 41 66 628.5 0 66 42 205 628.5 0 205 43 68 628.5 0 68 44 53 628.5 0 53 45 62 628.5 0 62 46 63 628.5 0 63 47 59 628.5 0 59 48 70 628.5 0 70 49 58 628.5 0 58 50 53 628.5 0 53 51 99 628.5 0 99 52 96 628.5 0 96 53 65 628.5 0 65 54 105 628.5 0 105 55 79 628.5 0 79 56 78 628.5 0 78 57 115 628.5 0 115 58 84 628.5 0 84 59 71 628.5 0 71 60 61 628.5 0 61 61 53 628.5 0 53 62 91 628.5 0 91 63 103 628.5 0 103 64 129 628.5 0 129 65 189 628.5 0 189 66 175 628.5 0 175 67 399 628.5 0 399 68 582 628.5 0 582 69 8408 628.5 0 8408 70 3501 628.5 0 3501 71 9013 628.5 0 9013 72 72 628.5 0 72 73 56 628.5 0 56 74 64 628.5 0 64 75 54 628.5 0 54 76 59 628.5 0 59 77 59 628.5 0 59 78 54 628.5 0 54 79 62 628.5 0 62 80 192 628.5 0 192 81 96 628.5 0 96 82 156 628.5 0 156 83 69 628.5 0 69 84 51 628.5 0 51 85 47 628.5 0 47 86 70 628.5 0 70 87 78 628.5 0 78 88 69 628.5 0 69 89 73 628.5 0 73 90 57 628.5 0 57 91 67 628.5 0 67 92 68 628.5 0 68 93 87 628.5 0 87 94 69 628.5 0 69 95 79 628.5 0 79 96 66 628.5 0 66 97 71 628.5 0 71 98 81 628.5 0 81 99 60 628.5 0 60 100 170 628.5 0 170 101 316 628.5 0 316 102 359 628.5 0 359 103 78 628.5 0 78 104 47 628.5 0 47 105 43 628.5 0 43 106 59 628.5 0 59 107 62 628.5 0 62 108 48 628.5 0 48 109 63 628.5 0 63 110 68 628.5 0 68 111 72 628.5 0 72 112 54 628.5 0 54 113 72 628.5 0 72 114 92 628.5 0 92 115 75 628.5 0 75 116 64 628.5 0 64 117 69 628.5 0 69 118 57 628.5 0 57 119 54 628.5 0 54 120 62 628.5 0 62 121 49 628.5 0 49 122 49 628.5 0 49 123 62 628.5 0 62 124 66 628.5 0 66 125 66 628.5 0 66 126 77 628.5 0 77 127 56 628.5 0 56 128 61 628.5 0 61 129 55 628.5 0 55 130 93 628.5 0 93 131 69 628.5 0 69 132 228 628.5 0 228 133 199 628.5 0 199 134 193 628.5 0 193 135 64 628.5 0 64 136 193 628.5 0 193 137 337 628.5 0 337 138 153 628.5 0 153 139 103 628.5 0 103 140 71 628.5 0 71 141 71 628.5 0 71 142 56 628.5 0 56 143 65 628.5 0 65 144 62 628.5 0 62 145 66 628.5 0 66 146 53 628.5 0 53 147 72 628.5 0 72 148 78 628.5 0 78 149 54 628.5 0 54 150 75 628.5 0 75 151 72 628.5 0 72 152 84 628.5 0 84 153 81 628.5 0 81 154 89 628.5 0 89 155 73 628.5 0 73 156 78 628.5 0 78 157 110 628.5 0 110 158 99 628.5 0 99 159 169 628.5 0 169 160 72 628.5 0 72 161 94 628.5 0 94 162 73 628.5 0 73 163 84 628.5 0 84 164 80 628.5 0 80 165 61 628.5 0 61 166 103 628.5 0 103 167 129 628.5 0 129 168 84 628.5 0 84 169 95 628.5 0 95 170 111 628.5 0 111 171 68 628.5 0 68 172 97 628.5 0 97 173 95 628.5 0 95 174 76 628.5 0 76 175 98 628.5 0 98 176 139 628.5 0 139 177 206 628.5 0 206 178 367 628.5 0 367 179 410 628.5 0 410 180 136 628.5 0 136 181 102 628.5 0 102 182 251 628.5 0 251 183 263 628.5 0 263 184 401 628.5 0 401 185 115 628.5 0 115 186 89 628.5 0 89 187 81 628.5 0 81 188 103 628.5 0 103 189 97 628.5 0 97 190 103 628.5 0 103 191 90 628.5 0 90 192 98 628.5 0 98 193 110 628.5 0 110 194 90 628.5 0 90 195 72 628.5 0 72 196 87 628.5 0 87 197 108 628.5 0 108 198 107 628.5 0 107 199 96 628.5 0 96 200 85 628.5 0 85 201 80 628.5 0 80 202 263 628.5 0 263 203 375 628.5 0 375 204 319 628.5 0 319 205 328 628.5 0 328 206 59 628.5 0 59 207 55 628.5 0 55 208 83 628.5 0 83 209 82 628.5 0 82 210 84 628.5 0 84 211 129 628.5 0 129 212 111 628.5 0 111 213 110 628.5 0 110 214 77 628.5 0 77 215 81 628.5 0 81 216 70 628.5 0 70 217 81 628.5 0 81 218 86 628.5 0 86 219 80 628.5 0 80 220 67 628.5 0 67 221 68 628.5 0 68 222 113 628.5 0 113 223 254 628.5 0 254 224 226 628.5 0 226 225 183 628.5 0 183 226 91 628.5 0 91 227 125 628.5 0 125 228 118 628.5 0 118 229 152 628.5 0 152 230 72 628.5 0 72 231 83 628.5 0 83 232 75 628.5 0 75 233 66 628.5 0 66 234 65 628.5 0 65 235 66 628.5 0 66 236 52 628.5 0 52 237 68 628.5 0 68 238 71 628.5 0 71 239 57 628.5 0 57 240 79 628.5 0 79 241 63 628.5 0 63 242 129 628.5 0 129 243 176 628.5 0 176 244 178 628.5 0 178 245 191 628.5 0 191 246 101 628.5 0 101 247 94 628.5 0 94 248 42 628.5 0 42 249 66 628.5 0 66 250 125 628.5 0 125 251 99 628.5 0 99 252 99 628.5 0 99 253 85 628.5 0 85 254 84 628.5 0 84 255 192 628.5 0 192 256 122 628.5 0 122 257 122 628.5 0 122 258 136 628.5 0 136 259 90 628.5 0 90 260 78 628.5 0 78 261 72 628.5 0 72 262 103 628.5 0 103 263 122 628.5 0 122 264 100 628.5 0 100 265 216 628.5 0 216 266 154 628.5 0 154 267 122 628.5 0 122 268 76 628.5 0 76 269 265 628.5 0 265 270 165 628.5 0 165 271 261 628.5 0 261 272 279 628.5 0 279 273 148 628.5 0 148 274 112 628.5 0 112 275 145 628.5 0 145 276 125 628.5 0 125 277 138 628.5 0 138 278 149 628.5 0 149 279 163 628.5 0 163 280 63 628.5 0 63 281 80 628.5 0 80 282 71 628.5 0 71 283 129 628.5 0 129 284 109 628.5 0 109 285 115 628.5 0 115 286 120 628.5 0 120 287 84 628.5 0 84 288 87 628.5 0 87 289 98 628.5 0 98 290 78 628.5 0 78 291 134 628.5 0 134 292 177 628.5 0 177 293 151 628.5 0 151 294 147 628.5 0 147 295 67 628.5 0 67 296 71 628.5 0 71 297 98 628.5 0 98 298 139 628.5 0 139 299 157 628.5 0 157 300 176 628.5 0 176 301 94 628.5 0 94 Finished in 3.87 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 86,676 Reads with adapters: 22,604 (26.1%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 22,579 (26.0%) Total basepairs processed: 22,108,935 bp Total written (filtered): 6,223,416 bp (28.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22604 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 3 2 16 182 0.0 3 0 179 3 17 21202 0.0 3 20792 399 10 1 18 62 0.0 3 11 50 0 1 19 22 0.0 3 2 9 10 1 20 1055 0.0 3 1031 13 1 10 21 2 0.0 3 0 2 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 1 32 2 0.0 3 1 0 0 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 55 13 0.0 3 0 0 0 13 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 13 0.0 3 0 0 0 13 92 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 1 3 118 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 220 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 22,579 Reads with adapters: 193 (0.9%) Reads written (passing filters): 22,579 (100.0%) Total basepairs processed: 6,223,416 bp Total written (filtered): 6,222,800 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 193 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.2% C: 5.2% G: 15.0% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 157 352.8 0 157 4 35 88.2 0 35 5 1 22.0 0 1