This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.46 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 267,095 (10.4%) Reads that were too short: 14,392 (0.6%) Reads written (passing filters): 252,703 (9.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 71,689,211 bp (9.3%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 267095 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3071 628.5 0 3071 7 106896 628.5 0 106896 8 6169 628.5 0 6169 9 111160 628.5 0 111160 10 274 628.5 0 274 11 78 628.5 0 78 12 65 628.5 0 65 13 90 628.5 0 90 14 118 628.5 0 118 15 93 628.5 0 93 16 79 628.5 0 79 17 80 628.5 0 80 18 76 628.5 0 76 19 100 628.5 0 100 20 126 628.5 0 126 21 126 628.5 0 126 22 188 628.5 0 188 23 456 628.5 0 456 24 350 628.5 0 350 25 523 628.5 0 523 26 74 628.5 0 74 27 71 628.5 0 71 28 71 628.5 0 71 29 83 628.5 0 83 30 82 628.5 0 82 31 62 628.5 0 62 32 85 628.5 0 85 33 81 628.5 0 81 34 93 628.5 0 93 35 125 628.5 0 125 36 107 628.5 0 107 37 80 628.5 0 80 38 79 628.5 0 79 39 78 628.5 0 78 40 80 628.5 0 80 41 57 628.5 0 57 42 68 628.5 0 68 43 66 628.5 0 66 44 70 628.5 0 70 45 78 628.5 0 78 46 65 628.5 0 65 47 62 628.5 0 62 48 62 628.5 0 62 49 66 628.5 0 66 50 62 628.5 0 62 51 46 628.5 0 46 52 59 628.5 0 59 53 59 628.5 0 59 54 66 628.5 0 66 55 71 628.5 0 71 56 82 628.5 0 82 57 58 628.5 0 58 58 65 628.5 0 65 59 62 628.5 0 62 60 75 628.5 0 75 61 62 628.5 0 62 62 54 628.5 0 54 63 78 628.5 0 78 64 73 628.5 0 73 65 73 628.5 0 73 66 74 628.5 0 74 67 74 628.5 0 74 68 70 628.5 0 70 69 62 628.5 0 62 70 73 628.5 0 73 71 98 628.5 0 98 72 64 628.5 0 64 73 270 628.5 0 270 74 99 628.5 0 99 75 70 628.5 0 70 76 54 628.5 0 54 77 56 628.5 0 56 78 72 628.5 0 72 79 62 628.5 0 62 80 74 628.5 0 74 81 97 628.5 0 97 82 105 628.5 0 105 83 77 628.5 0 77 84 115 628.5 0 115 85 431 628.5 0 431 86 111 628.5 0 111 87 114 628.5 0 114 88 971 628.5 0 971 89 1690 628.5 0 1690 90 796 628.5 0 796 91 57 628.5 0 57 92 53 628.5 0 53 93 55 628.5 0 55 94 91 628.5 0 91 95 82 628.5 0 82 96 268 628.5 0 268 97 289 628.5 0 289 98 223 628.5 0 223 99 125 628.5 0 125 100 163 628.5 0 163 101 909 628.5 0 909 102 457 628.5 0 457 103 712 628.5 0 712 104 67 628.5 0 67 105 95 628.5 0 95 106 77 628.5 0 77 107 44 628.5 0 44 108 50 628.5 0 50 109 76 628.5 0 76 110 119 628.5 0 119 111 97 628.5 0 97 112 87 628.5 0 87 113 82 628.5 0 82 114 85 628.5 0 85 115 83 628.5 0 83 116 57 628.5 0 57 117 85 628.5 0 85 118 67 628.5 0 67 119 76 628.5 0 76 120 50 628.5 0 50 121 63 628.5 0 63 122 84 628.5 0 84 123 94 628.5 0 94 124 75 628.5 0 75 125 244 628.5 0 244 126 135 628.5 0 135 127 1244 628.5 0 1244 128 425 628.5 0 425 129 477 628.5 0 477 130 95 628.5 0 95 131 85 628.5 0 85 132 59 628.5 0 59 133 93 628.5 0 93 134 75 628.5 0 75 135 88 628.5 0 88 136 67 628.5 0 67 137 65 628.5 0 65 138 77 628.5 0 77 139 58 628.5 0 58 140 85 628.5 0 85 141 99 628.5 0 99 142 76 628.5 0 76 143 103 628.5 0 103 144 527 628.5 0 527 145 96 628.5 0 96 146 85 628.5 0 85 147 196 628.5 0 196 148 274 628.5 0 274 149 195 628.5 0 195 150 52 628.5 0 52 151 59 628.5 0 59 152 77 628.5 0 77 153 79 628.5 0 79 154 56 628.5 0 56 155 52 628.5 0 52 156 68 628.5 0 68 157 61 628.5 0 61 158 87 628.5 0 87 159 86 628.5 0 86 160 52 628.5 0 52 161 69 628.5 0 69 162 47 628.5 0 47 163 51 628.5 0 51 164 52 628.5 0 52 165 47 628.5 0 47 166 35 628.5 0 35 167 68 628.5 0 68 168 72 628.5 0 72 169 84 628.5 0 84 170 57 628.5 0 57 171 76 628.5 0 76 172 48 628.5 0 48 173 36 628.5 0 36 174 41 628.5 0 41 175 52 628.5 0 52 176 97 628.5 0 97 177 82 628.5 0 82 178 82 628.5 0 82 179 51 628.5 0 51 180 52 628.5 0 52 181 78 628.5 0 78 182 103 628.5 0 103 183 65 628.5 0 65 184 49 628.5 0 49 185 47 628.5 0 47 186 63 628.5 0 63 187 67 628.5 0 67 188 50 628.5 0 50 189 59 628.5 0 59 190 78 628.5 0 78 191 39 628.5 0 39 192 76 628.5 0 76 193 85 628.5 0 85 194 95 628.5 0 95 195 252 628.5 0 252 196 174 628.5 0 174 197 102 628.5 0 102 198 56 628.5 0 56 199 56 628.5 0 56 200 97 628.5 0 97 201 177 628.5 0 177 202 151 628.5 0 151 203 109 628.5 0 109 204 99 628.5 0 99 205 84 628.5 0 84 206 72 628.5 0 72 207 134 628.5 0 134 208 123 628.5 0 123 209 198 628.5 0 198 210 97 628.5 0 97 211 78 628.5 0 78 212 68 628.5 0 68 213 82 628.5 0 82 214 95 628.5 0 95 215 109 628.5 0 109 216 86 628.5 0 86 217 88 628.5 0 88 218 367 628.5 0 367 219 75 628.5 0 75 220 66 628.5 0 66 221 64 628.5 0 64 222 79 628.5 0 79 223 125 628.5 0 125 224 112 628.5 0 112 225 79 628.5 0 79 226 81 628.5 0 81 227 61 628.5 0 61 228 74 628.5 0 74 229 75 628.5 0 75 230 78 628.5 0 78 231 113 628.5 0 113 232 121 628.5 0 121 233 85 628.5 0 85 234 107 628.5 0 107 235 128 628.5 0 128 236 116 628.5 0 116 237 95 628.5 0 95 238 159 628.5 0 159 239 267 628.5 0 267 240 262 628.5 0 262 241 194 628.5 0 194 242 136 628.5 0 136 243 71 628.5 0 71 244 62 628.5 0 62 245 60 628.5 0 60 246 68 628.5 0 68 247 74 628.5 0 74 248 75 628.5 0 75 249 81 628.5 0 81 250 81 628.5 0 81 251 65 628.5 0 65 252 64 628.5 0 64 253 62 628.5 0 62 254 91 628.5 0 91 255 106 628.5 0 106 256 144 628.5 0 144 257 92 628.5 0 92 258 111 628.5 0 111 259 1080 628.5 0 1080 260 1409 628.5 0 1409 261 1043 628.5 0 1043 262 516 628.5 0 516 263 256 628.5 0 256 264 51 628.5 0 51 265 61 628.5 0 61 266 72 628.5 0 72 267 59 628.5 0 59 268 75 628.5 0 75 269 76 628.5 0 76 270 50 628.5 0 50 271 45 628.5 0 45 272 71 628.5 0 71 273 89 628.5 0 89 274 76 628.5 0 76 275 90 628.5 0 90 276 71 628.5 0 71 277 61 628.5 0 61 278 77 628.5 0 77 279 320 628.5 0 320 280 382 628.5 0 382 281 596 628.5 0 596 282 74 628.5 0 74 283 72 628.5 0 72 284 77 628.5 0 77 285 39 628.5 0 39 286 70 628.5 0 70 287 42 628.5 0 42 288 72 628.5 0 72 289 71 628.5 0 71 290 66 628.5 0 66 291 109 628.5 0 109 292 124 628.5 0 124 293 139 628.5 0 139 294 100 628.5 0 100 295 105 628.5 0 105 296 124 628.5 0 124 297 62 628.5 0 62 298 74 628.5 0 74 299 70 628.5 0 70 300 86 628.5 0 86 301 91 628.5 0 91 Finished in 12.43 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 252,703 Reads with adapters: 109,694 (43.4%) Reads that were too short: 73 (0.0%) Reads written (passing filters): 109,621 (43.4%) Total basepairs processed: 71,689,211 bp Total written (filtered): 29,829,085 bp (41.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 109694 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 9 0.0 3 0 0 0 4 5 18 50 0.0 3 0 0 33 10 7 19 1520 0.0 3 0 1171 191 106 52 20 105519 0.0 4 97566 6270 1237 359 87 21 305 0.0 4 45 224 22 8 6 22 35 0.0 4 2 2 26 2 3 23 257 0.0 4 2 1 4 172 78 24 1 0.0 4 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 1 28 3 0.0 4 0 0 0 0 3 38 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 1 1 102 2 0.0 4 0 0 0 0 2 103 2 0.0 4 0 1 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 3 0.0 4 0 0 0 0 3 126 23 0.0 4 0 0 0 0 23 127 1806 0.0 4 0 0 0 0 1806 128 5 0.0 4 0 0 0 0 5 129 2 0.0 4 0 0 0 0 2 130 12 0.0 4 0 0 0 1 11 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 52 0.0 4 0 0 0 0 52 136 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 1 191 1 0.0 4 0 0 1 198 1 0.0 4 0 0 0 1 203 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 3 0.0 4 0 0 0 0 3 228 5 0.0 4 0 0 0 0 5 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 6 0.0 4 0 0 0 0 6 236 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 260 4 0.0 4 0 0 0 1 3 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 283 1 0.0 4 0 0 0 0 1 285 5 0.0 4 0 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.76 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 109,621 Reads with adapters: 6,268 (5.7%) Reads written (passing filters): 109,621 (100.0%) Total basepairs processed: 29,829,085 bp Total written (filtered): 29,748,275 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6268 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.9% C: 15.2% G: 71.4% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 757 1712.8 0 757 4 83 428.2 0 83 5 4 107.1 0 4 7 2 6.7 0 2 8 2 1.7 0 2 9 39 0.4 0 3 36 10 70 0.1 1 3 67 11 2143 0.0 1 110 2033 12 3011 0.0 1 88 2923 13 57 0.0 1 1 56 14 6 0.0 1 0 6 15 5 0.0 1 0 5 152 28 0.0 1 23 5 153 1 0.0 1 1 169 1 0.0 1 1 171 12 0.0 1 11 1 191 5 0.0 1 0 5 210 38 0.0 1 25 13 222 1 0.0 1 0 1 225 1 0.0 1 1 230 1 0.0 1 1 259 1 0.0 1 1