This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.52 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 269,361 (10.5%) Reads that were too short: 7,553 (0.3%) Reads written (passing filters): 261,808 (10.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 75,329,830 bp (9.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 269361 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3041 628.5 0 3041 7 130640 628.5 0 130640 8 3663 628.5 0 3663 9 109001 628.5 0 109001 10 193 628.5 0 193 11 58 628.5 0 58 12 37 628.5 0 37 13 40 628.5 0 40 14 41 628.5 0 41 15 38 628.5 0 38 16 37 628.5 0 37 17 48 628.5 0 48 18 40 628.5 0 40 19 41 628.5 0 41 20 45 628.5 0 45 21 53 628.5 0 53 22 83 628.5 0 83 23 461 628.5 0 461 24 258 628.5 0 258 25 570 628.5 0 570 26 28 628.5 0 28 27 29 628.5 0 29 28 27 628.5 0 27 29 32 628.5 0 32 30 40 628.5 0 40 31 39 628.5 0 39 32 39 628.5 0 39 33 35 628.5 0 35 34 38 628.5 0 38 35 34 628.5 0 34 36 35 628.5 0 35 37 39 628.5 0 39 38 32 628.5 0 32 39 31 628.5 0 31 40 44 628.5 0 44 41 36 628.5 0 36 42 35 628.5 0 35 43 35 628.5 0 35 44 39 628.5 0 39 45 23 628.5 0 23 46 35 628.5 0 35 47 23 628.5 0 23 48 27 628.5 0 27 49 21 628.5 0 21 50 35 628.5 0 35 51 35 628.5 0 35 52 30 628.5 0 30 53 23 628.5 0 23 54 35 628.5 0 35 55 37 628.5 0 37 56 30 628.5 0 30 57 28 628.5 0 28 58 30 628.5 0 30 59 22 628.5 0 22 60 27 628.5 0 27 61 32 628.5 0 32 62 20 628.5 0 20 63 22 628.5 0 22 64 30 628.5 0 30 65 18 628.5 0 18 66 34 628.5 0 34 67 23 628.5 0 23 68 33 628.5 0 33 69 30 628.5 0 30 70 32 628.5 0 32 71 54 628.5 0 54 72 41 628.5 0 41 73 267 628.5 0 267 74 52 628.5 0 52 75 38 628.5 0 38 76 33 628.5 0 33 77 25 628.5 0 25 78 40 628.5 0 40 79 24 628.5 0 24 80 35 628.5 0 35 81 59 628.5 0 59 82 41 628.5 0 41 83 39 628.5 0 39 84 59 628.5 0 59 85 398 628.5 0 398 86 81 628.5 0 81 87 61 628.5 0 61 88 528 628.5 0 528 89 1421 628.5 0 1421 90 497 628.5 0 497 91 19 628.5 0 19 92 22 628.5 0 22 93 21 628.5 0 21 94 31 628.5 0 31 95 22 628.5 0 22 96 60 628.5 0 60 97 60 628.5 0 60 98 41 628.5 0 41 99 53 628.5 0 53 100 68 628.5 0 68 101 554 628.5 0 554 102 352 628.5 0 352 103 493 628.5 0 493 104 43 628.5 0 43 105 45 628.5 0 45 106 45 628.5 0 45 107 22 628.5 0 22 108 36 628.5 0 36 109 22 628.5 0 22 110 51 628.5 0 51 111 51 628.5 0 51 112 34 628.5 0 34 113 51 628.5 0 51 114 42 628.5 0 42 115 32 628.5 0 32 116 21 628.5 0 21 117 51 628.5 0 51 118 29 628.5 0 29 119 34 628.5 0 34 120 33 628.5 0 33 121 32 628.5 0 32 122 44 628.5 0 44 123 29 628.5 0 29 124 45 628.5 0 45 125 154 628.5 0 154 126 61 628.5 0 61 127 1188 628.5 0 1188 128 371 628.5 0 371 129 454 628.5 0 454 130 38 628.5 0 38 131 30 628.5 0 30 132 34 628.5 0 34 133 45 628.5 0 45 134 35 628.5 0 35 135 36 628.5 0 36 136 26 628.5 0 26 137 26 628.5 0 26 138 23 628.5 0 23 139 16 628.5 0 16 140 37 628.5 0 37 141 42 628.5 0 42 142 40 628.5 0 40 143 43 628.5 0 43 144 638 628.5 0 638 145 69 628.5 0 69 146 50 628.5 0 50 147 184 628.5 0 184 148 245 628.5 0 245 149 151 628.5 0 151 150 26 628.5 0 26 151 42 628.5 0 42 152 25 628.5 0 25 153 33 628.5 0 33 154 19 628.5 0 19 155 23 628.5 0 23 156 32 628.5 0 32 157 40 628.5 0 40 158 32 628.5 0 32 159 27 628.5 0 27 160 30 628.5 0 30 161 39 628.5 0 39 162 28 628.5 0 28 163 26 628.5 0 26 164 26 628.5 0 26 165 22 628.5 0 22 166 21 628.5 0 21 167 41 628.5 0 41 168 22 628.5 0 22 169 46 628.5 0 46 170 25 628.5 0 25 171 17 628.5 0 17 172 23 628.5 0 23 173 12 628.5 0 12 174 16 628.5 0 16 175 18 628.5 0 18 176 38 628.5 0 38 177 34 628.5 0 34 178 32 628.5 0 32 179 27 628.5 0 27 180 25 628.5 0 25 181 27 628.5 0 27 182 35 628.5 0 35 183 30 628.5 0 30 184 29 628.5 0 29 185 26 628.5 0 26 186 37 628.5 0 37 187 36 628.5 0 36 188 24 628.5 0 24 189 29 628.5 0 29 190 40 628.5 0 40 191 17 628.5 0 17 192 29 628.5 0 29 193 39 628.5 0 39 194 46 628.5 0 46 195 56 628.5 0 56 196 41 628.5 0 41 197 26 628.5 0 26 198 17 628.5 0 17 199 25 628.5 0 25 200 45 628.5 0 45 201 80 628.5 0 80 202 51 628.5 0 51 203 39 628.5 0 39 204 32 628.5 0 32 205 37 628.5 0 37 206 30 628.5 0 30 207 32 628.5 0 32 208 58 628.5 0 58 209 96 628.5 0 96 210 29 628.5 0 29 211 38 628.5 0 38 212 31 628.5 0 31 213 35 628.5 0 35 214 43 628.5 0 43 215 37 628.5 0 37 216 38 628.5 0 38 217 38 628.5 0 38 218 382 628.5 0 382 219 33 628.5 0 33 220 26 628.5 0 26 221 26 628.5 0 26 222 35 628.5 0 35 223 45 628.5 0 45 224 40 628.5 0 40 225 43 628.5 0 43 226 38 628.5 0 38 227 32 628.5 0 32 228 28 628.5 0 28 229 35 628.5 0 35 230 36 628.5 0 36 231 45 628.5 0 45 232 32 628.5 0 32 233 30 628.5 0 30 234 65 628.5 0 65 235 46 628.5 0 46 236 132 628.5 0 132 237 68 628.5 0 68 238 69 628.5 0 69 239 132 628.5 0 132 240 101 628.5 0 101 241 60 628.5 0 60 242 35 628.5 0 35 243 46 628.5 0 46 244 40 628.5 0 40 245 19 628.5 0 19 246 45 628.5 0 45 247 45 628.5 0 45 248 48 628.5 0 48 249 50 628.5 0 50 250 46 628.5 0 46 251 40 628.5 0 40 252 32 628.5 0 32 253 30 628.5 0 30 254 57 628.5 0 57 255 77 628.5 0 77 256 81 628.5 0 81 257 48 628.5 0 48 258 67 628.5 0 67 259 820 628.5 0 820 260 777 628.5 0 777 261 404 628.5 0 404 262 340 628.5 0 340 263 95 628.5 0 95 264 21 628.5 0 21 265 31 628.5 0 31 266 42 628.5 0 42 267 38 628.5 0 38 268 35 628.5 0 35 269 29 628.5 0 29 270 19 628.5 0 19 271 22 628.5 0 22 272 24 628.5 0 24 273 43 628.5 0 43 274 29 628.5 0 29 275 28 628.5 0 28 276 47 628.5 0 47 277 31 628.5 0 31 278 30 628.5 0 30 279 158 628.5 0 158 280 245 628.5 0 245 281 366 628.5 0 366 282 39 628.5 0 39 283 29 628.5 0 29 284 42 628.5 0 42 285 29 628.5 0 29 286 41 628.5 0 41 287 30 628.5 0 30 288 26 628.5 0 26 289 32 628.5 0 32 290 22 628.5 0 22 291 38 628.5 0 38 292 42 628.5 0 42 293 65 628.5 0 65 294 58 628.5 0 58 295 61 628.5 0 61 296 62 628.5 0 62 297 58 628.5 0 58 298 32 628.5 0 32 299 33 628.5 0 33 300 49 628.5 0 49 301 42 628.5 0 42 Finished in 11.66 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 261,808 Reads with adapters: 113,756 (43.5%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 113,714 (43.4%) Total basepairs processed: 75,329,830 bp Total written (filtered): 31,270,559 bp (41.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 113756 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 25 0.0 3 0 0 24 1 16 746 0.0 3 0 725 21 17 112571 0.0 3 110423 2070 67 11 18 204 0.0 3 8 184 9 3 19 54 0.0 3 4 1 42 7 20 59 0.0 3 0 0 0 59 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 1 32 1 0.0 3 1 34 1 0.0 3 0 0 0 1 40 8 0.0 3 0 0 6 2 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 64 8 0.0 3 0 0 0 8 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 72 1 0.0 3 1 73 1 0.0 3 0 0 0 1 75 6 0.0 3 0 0 0 6 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 93 1 0.0 3 1 98 4 0.0 3 1 1 0 2 99 2 0.0 3 2 100 1 0.0 3 1 103 7 0.0 3 0 0 0 7 104 10 0.0 3 0 0 0 10 109 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 1 0.0 3 0 1 155 1 0.0 3 0 0 1 159 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 1 206 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.06 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 113,714 Reads with adapters: 3,417 (3.0%) Reads written (passing filters): 113,714 (100.0%) Total basepairs processed: 31,270,559 bp Total written (filtered): 31,260,036 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3417 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.7% C: 6.5% G: 17.1% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3157 1776.8 0 3157 4 256 444.2 0 256 5 2 111.0 0 2 6 1 27.8 0 1 12 1 0.0 1 0 1