This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.61 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 136,074 (5.3%) Reads that were too short: 18,611 (0.7%) Reads written (passing filters): 117,463 (4.6%) Total basepairs processed: 774,927,510 bp Total written (filtered): 32,749,967 bp (4.2%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 136074 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 745 628.5 0 745 7 2738 628.5 0 2738 8 56961 628.5 0 56961 9 41139 628.5 0 41139 10 178 628.5 0 178 11 50 628.5 0 50 12 15 628.5 0 15 13 30 628.5 0 30 14 37 628.5 0 37 15 112 628.5 0 112 16 38 628.5 0 38 17 75 628.5 0 75 18 73 628.5 0 73 19 96 628.5 0 96 20 106 628.5 0 106 21 33 628.5 0 33 22 28 628.5 0 28 23 33 628.5 0 33 24 39 628.5 0 39 25 31 628.5 0 31 26 22 628.5 0 22 27 24 628.5 0 24 28 84 628.5 0 84 29 208 628.5 0 208 30 103 628.5 0 103 31 23 628.5 0 23 32 25 628.5 0 25 33 32 628.5 0 32 34 44 628.5 0 44 35 50 628.5 0 50 36 46 628.5 0 46 37 84 628.5 0 84 38 28 628.5 0 28 39 32 628.5 0 32 40 39 628.5 0 39 41 26 628.5 0 26 42 33 628.5 0 33 43 107 628.5 0 107 44 108 628.5 0 108 45 479 628.5 0 479 46 433 628.5 0 433 47 524 628.5 0 524 48 26 628.5 0 26 49 24 628.5 0 24 50 26 628.5 0 26 51 24 628.5 0 24 52 17 628.5 0 17 53 13 628.5 0 13 54 15 628.5 0 15 55 16 628.5 0 16 56 15 628.5 0 15 57 20 628.5 0 20 58 20 628.5 0 20 59 18 628.5 0 18 60 17 628.5 0 17 61 27 628.5 0 27 62 24 628.5 0 24 63 23 628.5 0 23 64 37 628.5 0 37 65 18 628.5 0 18 66 25 628.5 0 25 67 23 628.5 0 23 68 84 628.5 0 84 69 32 628.5 0 32 70 68 628.5 0 68 71 165 628.5 0 165 72 56 628.5 0 56 73 109 628.5 0 109 74 45 628.5 0 45 75 16 628.5 0 16 76 21 628.5 0 21 77 16 628.5 0 16 78 26 628.5 0 26 79 36 628.5 0 36 80 111 628.5 0 111 81 42 628.5 0 42 82 36 628.5 0 36 83 53 628.5 0 53 84 66 628.5 0 66 85 44 628.5 0 44 86 37 628.5 0 37 87 26 628.5 0 26 88 23 628.5 0 23 89 14 628.5 0 14 90 26 628.5 0 26 91 29 628.5 0 29 92 26 628.5 0 26 93 50 628.5 0 50 94 564 628.5 0 564 95 521 628.5 0 521 96 184 628.5 0 184 97 333 628.5 0 333 98 30 628.5 0 30 99 22 628.5 0 22 100 15 628.5 0 15 101 19 628.5 0 19 102 26 628.5 0 26 103 19 628.5 0 19 104 27 628.5 0 27 105 15 628.5 0 15 106 13 628.5 0 13 107 24 628.5 0 24 108 17 628.5 0 17 109 21 628.5 0 21 110 36 628.5 0 36 111 47 628.5 0 47 112 88 628.5 0 88 113 502 628.5 0 502 114 347 628.5 0 347 115 432 628.5 0 432 116 68 628.5 0 68 117 43 628.5 0 43 118 20 628.5 0 20 119 26 628.5 0 26 120 29 628.5 0 29 121 27 628.5 0 27 122 42 628.5 0 42 123 56 628.5 0 56 124 73 628.5 0 73 125 44 628.5 0 44 126 32 628.5 0 32 127 37 628.5 0 37 128 32 628.5 0 32 129 34 628.5 0 34 130 43 628.5 0 43 131 33 628.5 0 33 132 52 628.5 0 52 133 40 628.5 0 40 134 47 628.5 0 47 135 166 628.5 0 166 136 142 628.5 0 142 137 373 628.5 0 373 138 467 628.5 0 467 139 259 628.5 0 259 140 355 628.5 0 355 141 54 628.5 0 54 142 191 628.5 0 191 143 165 628.5 0 165 144 96 628.5 0 96 145 31 628.5 0 31 146 22 628.5 0 22 147 34 628.5 0 34 148 46 628.5 0 46 149 86 628.5 0 86 150 40 628.5 0 40 151 37 628.5 0 37 152 19 628.5 0 19 153 34 628.5 0 34 154 29 628.5 0 29 155 35 628.5 0 35 156 36 628.5 0 36 157 18 628.5 0 18 158 29 628.5 0 29 159 21 628.5 0 21 160 18 628.5 0 18 161 26 628.5 0 26 162 62 628.5 0 62 163 71 628.5 0 71 164 116 628.5 0 116 165 59 628.5 0 59 166 37 628.5 0 37 167 115 628.5 0 115 168 207 628.5 0 207 169 343 628.5 0 343 170 504 628.5 0 504 171 28 628.5 0 28 172 37 628.5 0 37 173 33 628.5 0 33 174 33 628.5 0 33 175 26 628.5 0 26 176 18 628.5 0 18 177 26 628.5 0 26 178 35 628.5 0 35 179 67 628.5 0 67 180 108 628.5 0 108 181 86 628.5 0 86 182 48 628.5 0 48 183 79 628.5 0 79 184 187 628.5 0 187 185 361 628.5 0 361 186 368 628.5 0 368 187 72 628.5 0 72 188 44 628.5 0 44 189 43 628.5 0 43 190 51 628.5 0 51 191 52 628.5 0 52 192 47 628.5 0 47 193 28 628.5 0 28 194 31 628.5 0 31 195 18 628.5 0 18 196 22 628.5 0 22 197 24 628.5 0 24 198 41 628.5 0 41 199 79 628.5 0 79 200 74 628.5 0 74 201 78 628.5 0 78 202 60 628.5 0 60 203 61 628.5 0 61 204 319 628.5 0 319 205 451 628.5 0 451 206 472 628.5 0 472 207 191 628.5 0 191 208 65 628.5 0 65 209 87 628.5 0 87 210 132 628.5 0 132 211 160 628.5 0 160 212 68 628.5 0 68 213 82 628.5 0 82 214 58 628.5 0 58 215 112 628.5 0 112 216 134 628.5 0 134 217 106 628.5 0 106 218 63 628.5 0 63 219 78 628.5 0 78 220 77 628.5 0 77 221 77 628.5 0 77 222 84 628.5 0 84 223 147 628.5 0 147 224 264 628.5 0 264 225 2126 628.5 0 2126 226 1899 628.5 0 1899 227 3500 628.5 0 3500 228 3247 628.5 0 3247 229 74 628.5 0 74 230 51 628.5 0 51 231 40 628.5 0 40 232 45 628.5 0 45 233 36 628.5 0 36 234 77 628.5 0 77 235 126 628.5 0 126 236 144 628.5 0 144 237 85 628.5 0 85 238 82 628.5 0 82 239 47 628.5 0 47 240 66 628.5 0 66 241 59 628.5 0 59 242 40 628.5 0 40 243 45 628.5 0 45 244 41 628.5 0 41 245 46 628.5 0 46 246 42 628.5 0 42 247 53 628.5 0 53 248 78 628.5 0 78 249 38 628.5 0 38 250 37 628.5 0 37 251 26 628.5 0 26 252 25 628.5 0 25 253 51 628.5 0 51 254 42 628.5 0 42 255 38 628.5 0 38 256 37 628.5 0 37 257 45 628.5 0 45 258 60 628.5 0 60 259 63 628.5 0 63 260 69 628.5 0 69 261 70 628.5 0 70 262 38 628.5 0 38 263 45 628.5 0 45 264 98 628.5 0 98 265 107 628.5 0 107 266 111 628.5 0 111 267 57 628.5 0 57 268 34 628.5 0 34 269 66 628.5 0 66 270 57 628.5 0 57 271 37 628.5 0 37 272 34 628.5 0 34 273 50 628.5 0 50 274 61 628.5 0 61 275 121 628.5 0 121 276 112 628.5 0 112 277 26 628.5 0 26 278 30 628.5 0 30 279 38 628.5 0 38 280 68 628.5 0 68 281 53 628.5 0 53 282 55 628.5 0 55 283 34 628.5 0 34 284 31 628.5 0 31 285 36 628.5 0 36 286 27 628.5 0 27 287 42 628.5 0 42 288 33 628.5 0 33 289 43 628.5 0 43 290 46 628.5 0 46 291 42 628.5 0 42 292 42 628.5 0 42 293 78 628.5 0 78 294 66 628.5 0 66 295 75 628.5 0 75 296 113 628.5 0 113 297 198 628.5 0 198 298 90 628.5 0 90 299 93 628.5 0 93 300 55 628.5 0 55 301 171 628.5 0 171 Finished in 6.19 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 117,463 Reads with adapters: 45,353 (38.6%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 45,289 (38.6%) Total basepairs processed: 32,749,967 bp Total written (filtered): 12,305,097 bp (37.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45353 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 4 3 18 42 0.0 3 0 0 14 15 13 19 541 0.0 3 0 388 48 72 33 20 44049 0.0 4 39796 3220 719 236 78 21 149 0.0 4 36 91 17 4 1 22 17 0.0 4 2 1 10 2 2 23 312 0.0 4 1 0 0 197 114 24 2 0.0 4 1 0 0 0 1 25 2 0.0 4 1 0 0 0 1 42 1 0.0 4 0 0 0 1 47 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 89 26 0.0 4 0 0 0 0 26 91 4 0.0 4 0 0 0 0 4 92 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 1 97 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 105 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 127 109 0.0 4 0 0 0 0 109 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 11 0.0 4 1 0 0 0 10 168 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 1 176 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 225 2 0.0 4 0 0 0 0 2 228 5 0.0 4 0 0 0 0 5 229 5 0.0 4 0 0 0 0 5 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 7 0.0 4 0 0 0 0 7 235 6 0.0 4 0 0 0 0 6 237 5 0.0 4 0 0 0 0 5 245 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.67 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 45,289 Reads with adapters: 11,103 (24.5%) Reads written (passing filters): 45,289 (100.0%) Total basepairs processed: 12,305,097 bp Total written (filtered): 12,173,726 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11103 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.5% C: 9.5% G: 84.6% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 35 707.6 0 35 4 13 176.9 0 13 5 1 44.2 0 1 6 2 11.1 0 2 7 2 2.8 0 2 8 10 0.7 0 10 9 2464 0.2 0 365 2099 10 6470 0.0 1 549 5921 11 1899 0.0 1 85 1814 12 41 0.0 1 1 40 13 28 0.0 1 1 27 14 3 0.0 1 0 3 23 19 0.0 1 12 7 24 2 0.0 1 2 71 1 0.0 1 0 1 106 1 0.0 1 1 107 2 0.0 1 2 109 1 0.0 1 1 149 1 0.0 1 1 150 19 0.0 1 15 4 151 1 0.0 1 1 189 1 0.0 1 0 1 207 1 0.0 1 1 208 71 0.0 1 62 9 209 11 0.0 1 10 1 210 2 0.0 1 1 1 211 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.