This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.58 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 125,919 (4.9%) Reads that were too short: 10,243 (0.4%) Reads written (passing filters): 115,676 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 32,847,948 bp (4.2%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 125919 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 552 628.5 0 552 7 1374 628.5 0 1374 8 52257 628.5 0 52257 9 51809 628.5 0 51809 10 104 628.5 0 104 11 37 628.5 0 37 12 22 628.5 0 22 13 20 628.5 0 20 14 16 628.5 0 16 15 12 628.5 0 12 16 15 628.5 0 15 17 12 628.5 0 12 18 41 628.5 0 41 19 30 628.5 0 30 20 29 628.5 0 29 21 13 628.5 0 13 22 20 628.5 0 20 23 22 628.5 0 22 24 14 628.5 0 14 25 13 628.5 0 13 26 14 628.5 0 14 27 15 628.5 0 15 28 25 628.5 0 25 29 26 628.5 0 26 30 40 628.5 0 40 31 23 628.5 0 23 32 25 628.5 0 25 33 20 628.5 0 20 34 27 628.5 0 27 35 21 628.5 0 21 36 24 628.5 0 24 37 35 628.5 0 35 38 11 628.5 0 11 39 13 628.5 0 13 40 14 628.5 0 14 41 13 628.5 0 13 42 23 628.5 0 23 43 49 628.5 0 49 44 39 628.5 0 39 45 193 628.5 0 193 46 238 628.5 0 238 47 289 628.5 0 289 48 19 628.5 0 19 49 18 628.5 0 18 50 17 628.5 0 17 51 16 628.5 0 16 52 28 628.5 0 28 53 24 628.5 0 24 54 18 628.5 0 18 55 13 628.5 0 13 56 26 628.5 0 26 57 17 628.5 0 17 58 18 628.5 0 18 59 16 628.5 0 16 60 12 628.5 0 12 61 19 628.5 0 19 62 21 628.5 0 21 63 10 628.5 0 10 64 21 628.5 0 21 65 20 628.5 0 20 66 19 628.5 0 19 67 18 628.5 0 18 68 70 628.5 0 70 69 17 628.5 0 17 70 28 628.5 0 28 71 36 628.5 0 36 72 28 628.5 0 28 73 53 628.5 0 53 74 17 628.5 0 17 75 15 628.5 0 15 76 16 628.5 0 16 77 26 628.5 0 26 78 28 628.5 0 28 79 43 628.5 0 43 80 29 628.5 0 29 81 23 628.5 0 23 82 24 628.5 0 24 83 16 628.5 0 16 84 28 628.5 0 28 85 23 628.5 0 23 86 18 628.5 0 18 87 28 628.5 0 28 88 31 628.5 0 31 89 24 628.5 0 24 90 29 628.5 0 29 91 29 628.5 0 29 92 39 628.5 0 39 93 50 628.5 0 50 94 643 628.5 0 643 95 593 628.5 0 593 96 174 628.5 0 174 97 350 628.5 0 350 98 18 628.5 0 18 99 28 628.5 0 28 100 22 628.5 0 22 101 25 628.5 0 25 102 11 628.5 0 11 103 22 628.5 0 22 104 10 628.5 0 10 105 8 628.5 0 8 106 18 628.5 0 18 107 12 628.5 0 12 108 19 628.5 0 19 109 13 628.5 0 13 110 20 628.5 0 20 111 20 628.5 0 20 112 58 628.5 0 58 113 128 628.5 0 128 114 135 628.5 0 135 115 218 628.5 0 218 116 49 628.5 0 49 117 21 628.5 0 21 118 7 628.5 0 7 119 18 628.5 0 18 120 35 628.5 0 35 121 19 628.5 0 19 122 23 628.5 0 23 123 17 628.5 0 17 124 73 628.5 0 73 125 23 628.5 0 23 126 25 628.5 0 25 127 20 628.5 0 20 128 28 628.5 0 28 129 24 628.5 0 24 130 23 628.5 0 23 131 20 628.5 0 20 132 37 628.5 0 37 133 29 628.5 0 29 134 34 628.5 0 34 135 119 628.5 0 119 136 75 628.5 0 75 137 302 628.5 0 302 138 400 628.5 0 400 139 212 628.5 0 212 140 414 628.5 0 414 141 28 628.5 0 28 142 109 628.5 0 109 143 68 628.5 0 68 144 35 628.5 0 35 145 29 628.5 0 29 146 17 628.5 0 17 147 12 628.5 0 12 148 22 628.5 0 22 149 30 628.5 0 30 150 23 628.5 0 23 151 24 628.5 0 24 152 20 628.5 0 20 153 12 628.5 0 12 154 19 628.5 0 19 155 23 628.5 0 23 156 27 628.5 0 27 157 19 628.5 0 19 158 31 628.5 0 31 159 19 628.5 0 19 160 21 628.5 0 21 161 22 628.5 0 22 162 43 628.5 0 43 163 52 628.5 0 52 164 32 628.5 0 32 165 25 628.5 0 25 166 18 628.5 0 18 167 42 628.5 0 42 168 101 628.5 0 101 169 162 628.5 0 162 170 201 628.5 0 201 171 19 628.5 0 19 172 17 628.5 0 17 173 19 628.5 0 19 174 29 628.5 0 29 175 14 628.5 0 14 176 15 628.5 0 15 177 31 628.5 0 31 178 24 628.5 0 24 179 20 628.5 0 20 180 26 628.5 0 26 181 25 628.5 0 25 182 32 628.5 0 32 183 32 628.5 0 32 184 68 628.5 0 68 185 131 628.5 0 131 186 132 628.5 0 132 187 49 628.5 0 49 188 38 628.5 0 38 189 21 628.5 0 21 190 34 628.5 0 34 191 28 628.5 0 28 192 24 628.5 0 24 193 22 628.5 0 22 194 16 628.5 0 16 195 22 628.5 0 22 196 13 628.5 0 13 197 28 628.5 0 28 198 41 628.5 0 41 199 49 628.5 0 49 200 27 628.5 0 27 201 25 628.5 0 25 202 29 628.5 0 29 203 31 628.5 0 31 204 101 628.5 0 101 205 136 628.5 0 136 206 119 628.5 0 119 207 84 628.5 0 84 208 36 628.5 0 36 209 27 628.5 0 27 210 54 628.5 0 54 211 81 628.5 0 81 212 27 628.5 0 27 213 48 628.5 0 48 214 32 628.5 0 32 215 45 628.5 0 45 216 72 628.5 0 72 217 35 628.5 0 35 218 29 628.5 0 29 219 34 628.5 0 34 220 46 628.5 0 46 221 42 628.5 0 42 222 52 628.5 0 52 223 95 628.5 0 95 224 180 628.5 0 180 225 1166 628.5 0 1166 226 1164 628.5 0 1164 227 2117 628.5 0 2117 228 2103 628.5 0 2103 229 36 628.5 0 36 230 19 628.5 0 19 231 35 628.5 0 35 232 21 628.5 0 21 233 20 628.5 0 20 234 33 628.5 0 33 235 36 628.5 0 36 236 46 628.5 0 46 237 41 628.5 0 41 238 27 628.5 0 27 239 29 628.5 0 29 240 29 628.5 0 29 241 31 628.5 0 31 242 26 628.5 0 26 243 25 628.5 0 25 244 16 628.5 0 16 245 18 628.5 0 18 246 18 628.5 0 18 247 29 628.5 0 29 248 26 628.5 0 26 249 25 628.5 0 25 250 28 628.5 0 28 251 17 628.5 0 17 252 21 628.5 0 21 253 31 628.5 0 31 254 34 628.5 0 34 255 31 628.5 0 31 256 36 628.5 0 36 257 30 628.5 0 30 258 33 628.5 0 33 259 35 628.5 0 35 260 33 628.5 0 33 261 24 628.5 0 24 262 21 628.5 0 21 263 25 628.5 0 25 264 47 628.5 0 47 265 43 628.5 0 43 266 29 628.5 0 29 267 32 628.5 0 32 268 29 628.5 0 29 269 34 628.5 0 34 270 28 628.5 0 28 271 26 628.5 0 26 272 19 628.5 0 19 273 24 628.5 0 24 274 29 628.5 0 29 275 28 628.5 0 28 276 27 628.5 0 27 277 25 628.5 0 25 278 32 628.5 0 32 279 31 628.5 0 31 280 33 628.5 0 33 281 31 628.5 0 31 282 22 628.5 0 22 283 20 628.5 0 20 284 23 628.5 0 23 285 22 628.5 0 22 286 28 628.5 0 28 287 20 628.5 0 20 288 28 628.5 0 28 289 28 628.5 0 28 290 35 628.5 0 35 291 27 628.5 0 27 292 36 628.5 0 36 293 44 628.5 0 44 294 34 628.5 0 34 295 46 628.5 0 46 296 48 628.5 0 48 297 84 628.5 0 84 298 35 628.5 0 35 299 35 628.5 0 35 300 27 628.5 0 27 301 84 628.5 0 84 Finished in 5.51 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 115,676 Reads with adapters: 49,881 (43.1%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 49,842 (43.1%) Total basepairs processed: 32,847,948 bp Total written (filtered): 13,752,574 bp (41.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49881 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 4 16 268 0.0 3 0 261 5 2 17 49330 0.0 3 48497 797 31 5 18 131 0.0 3 23 104 4 19 22 0.0 3 1 0 18 3 20 51 0.0 3 0 0 0 51 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 26 1 0.0 3 1 28 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 7 0.0 3 0 0 0 7 56 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 112 1 0.0 3 0 1 114 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 142 1 0.0 3 1 144 1 0.0 3 0 1 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 217 18 0.0 3 0 0 0 18 237 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 49,842 Reads with adapters: 229 (0.5%) Reads written (passing filters): 49,842 (100.0%) Total basepairs processed: 13,752,574 bp Total written (filtered): 13,751,790 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 229 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.8% C: 9.6% G: 28.8% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 139 778.8 0 139 4 89 194.7 0 89 11 1 0.0 1 0 1