This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.51 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 621,513 (24.1%) Reads that were too short: 13,807 (0.5%) Reads written (passing filters): 607,706 (23.6%) Total basepairs processed: 774,927,510 bp Total written (filtered): 129,522,087 bp (16.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 621513 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3003 628.5 0 3003 7 125166 628.5 0 125166 8 104424 628.5 0 104424 9 191 628.5 0 191 10 127 628.5 0 127 11 117 628.5 0 117 12 91 628.5 0 91 13 101 628.5 0 101 14 103 628.5 0 103 15 117 628.5 0 117 16 99 628.5 0 99 17 59 628.5 0 59 18 86 628.5 0 86 19 86 628.5 0 86 20 68 628.5 0 68 21 81 628.5 0 81 22 99 628.5 0 99 23 107 628.5 0 107 24 84 628.5 0 84 25 231 628.5 0 231 26 197 628.5 0 197 27 253 628.5 0 253 28 124 628.5 0 124 29 79 628.5 0 79 30 108 628.5 0 108 31 79 628.5 0 79 32 68 628.5 0 68 33 66 628.5 0 66 34 94 628.5 0 94 35 124 628.5 0 124 36 106 628.5 0 106 37 110 628.5 0 110 38 80 628.5 0 80 39 92 628.5 0 92 40 81 628.5 0 81 41 75 628.5 0 75 42 105 628.5 0 105 43 117 628.5 0 117 44 72 628.5 0 72 45 89 628.5 0 89 46 110 628.5 0 110 47 50 628.5 0 50 48 105 628.5 0 105 49 101 628.5 0 101 50 81 628.5 0 81 51 119 628.5 0 119 52 111 628.5 0 111 53 124 628.5 0 124 54 91 628.5 0 91 55 84 628.5 0 84 56 119 628.5 0 119 57 71 628.5 0 71 58 81 628.5 0 81 59 83 628.5 0 83 60 106 628.5 0 106 61 172 628.5 0 172 62 110 628.5 0 110 63 127 628.5 0 127 64 120 628.5 0 120 65 104 628.5 0 104 66 102 628.5 0 102 67 111 628.5 0 111 68 137 628.5 0 137 69 161 628.5 0 161 70 145 628.5 0 145 71 122 628.5 0 122 72 238 628.5 0 238 73 120 628.5 0 120 74 513 628.5 0 513 75 220 628.5 0 220 76 231 628.5 0 231 77 260 628.5 0 260 78 155 628.5 0 155 79 305 628.5 0 305 80 293 628.5 0 293 81 1200 628.5 0 1200 82 859 628.5 0 859 83 1837 628.5 0 1837 84 248 628.5 0 248 85 310 628.5 0 310 86 455 628.5 0 455 87 114 628.5 0 114 88 102 628.5 0 102 89 114 628.5 0 114 90 111 628.5 0 111 91 90 628.5 0 90 92 115 628.5 0 115 93 145 628.5 0 145 94 154 628.5 0 154 95 159 628.5 0 159 96 146 628.5 0 146 97 127 628.5 0 127 98 144 628.5 0 144 99 145 628.5 0 145 100 161 628.5 0 161 101 225 628.5 0 225 102 237 628.5 0 237 103 299 628.5 0 299 104 358 628.5 0 358 105 610 628.5 0 610 106 263 628.5 0 263 107 310 628.5 0 310 108 557 628.5 0 557 109 297 628.5 0 297 110 322 628.5 0 322 111 523 628.5 0 523 112 607 628.5 0 607 113 599 628.5 0 599 114 611 628.5 0 611 115 921 628.5 0 921 116 1082 628.5 0 1082 117 1243 628.5 0 1243 118 13343 628.5 0 13343 119 32457 628.5 0 32457 120 31896 628.5 0 31896 121 1927 628.5 0 1927 122 3215 628.5 0 3215 123 3960 628.5 0 3960 124 471 628.5 0 471 125 287 628.5 0 287 126 359 628.5 0 359 127 460 628.5 0 460 128 535 628.5 0 535 129 349 628.5 0 349 130 465 628.5 0 465 131 753 628.5 0 753 132 1333 628.5 0 1333 133 624 628.5 0 624 134 693 628.5 0 693 135 972 628.5 0 972 136 673 628.5 0 673 137 803 628.5 0 803 138 1226 628.5 0 1226 139 2757 628.5 0 2757 140 9692 628.5 0 9692 141 10434 628.5 0 10434 142 8388 628.5 0 8388 143 2373 628.5 0 2373 144 4510 628.5 0 4510 145 31544 628.5 0 31544 146 49387 628.5 0 49387 147 69675 628.5 0 69675 148 7079 628.5 0 7079 149 4796 628.5 0 4796 150 1213 628.5 0 1213 151 921 628.5 0 921 152 874 628.5 0 874 153 755 628.5 0 755 154 699 628.5 0 699 155 742 628.5 0 742 156 440 628.5 0 440 157 822 628.5 0 822 158 1063 628.5 0 1063 159 1007 628.5 0 1007 160 410 628.5 0 410 161 454 628.5 0 454 162 569 628.5 0 569 163 837 628.5 0 837 164 988 628.5 0 988 165 7560 628.5 0 7560 166 12185 628.5 0 12185 167 14940 628.5 0 14940 168 1141 628.5 0 1141 169 294 628.5 0 294 170 301 628.5 0 301 171 140 628.5 0 140 172 112 628.5 0 112 173 152 628.5 0 152 174 192 628.5 0 192 175 119 628.5 0 119 176 95 628.5 0 95 177 110 628.5 0 110 178 119 628.5 0 119 179 99 628.5 0 99 180 86 628.5 0 86 181 140 628.5 0 140 182 124 628.5 0 124 183 124 628.5 0 124 184 136 628.5 0 136 185 112 628.5 0 112 186 139 628.5 0 139 187 101 628.5 0 101 188 129 628.5 0 129 189 115 628.5 0 115 190 161 628.5 0 161 191 129 628.5 0 129 192 176 628.5 0 176 193 259 628.5 0 259 194 357 628.5 0 357 195 270 628.5 0 270 196 103 628.5 0 103 197 128 628.5 0 128 198 128 628.5 0 128 199 175 628.5 0 175 200 143 628.5 0 143 201 141 628.5 0 141 202 113 628.5 0 113 203 101 628.5 0 101 204 136 628.5 0 136 205 154 628.5 0 154 206 205 628.5 0 205 207 157 628.5 0 157 208 129 628.5 0 129 209 150 628.5 0 150 210 134 628.5 0 134 211 139 628.5 0 139 212 143 628.5 0 143 213 117 628.5 0 117 214 104 628.5 0 104 215 109 628.5 0 109 216 336 628.5 0 336 217 428 628.5 0 428 218 604 628.5 0 604 219 388 628.5 0 388 220 262 628.5 0 262 221 126 628.5 0 126 222 98 628.5 0 98 223 118 628.5 0 118 224 112 628.5 0 112 225 165 628.5 0 165 226 386 628.5 0 386 227 171 628.5 0 171 228 167 628.5 0 167 229 90 628.5 0 90 230 82 628.5 0 82 231 102 628.5 0 102 232 107 628.5 0 107 233 92 628.5 0 92 234 83 628.5 0 83 235 92 628.5 0 92 236 86 628.5 0 86 237 78 628.5 0 78 238 95 628.5 0 95 239 102 628.5 0 102 240 109 628.5 0 109 241 111 628.5 0 111 242 101 628.5 0 101 243 109 628.5 0 109 244 150 628.5 0 150 245 119 628.5 0 119 246 121 628.5 0 121 247 131 628.5 0 131 248 113 628.5 0 113 249 85 628.5 0 85 250 89 628.5 0 89 251 72 628.5 0 72 252 94 628.5 0 94 253 109 628.5 0 109 254 144 628.5 0 144 255 133 628.5 0 133 256 109 628.5 0 109 257 128 628.5 0 128 258 111 628.5 0 111 259 113 628.5 0 113 260 106 628.5 0 106 261 97 628.5 0 97 262 157 628.5 0 157 263 133 628.5 0 133 264 133 628.5 0 133 265 142 628.5 0 142 266 261 628.5 0 261 267 162 628.5 0 162 268 108 628.5 0 108 269 111 628.5 0 111 270 93 628.5 0 93 271 112 628.5 0 112 272 105 628.5 0 105 273 115 628.5 0 115 274 121 628.5 0 121 275 131 628.5 0 131 276 97 628.5 0 97 277 114 628.5 0 114 278 143 628.5 0 143 279 142 628.5 0 142 280 123 628.5 0 123 281 124 628.5 0 124 282 103 628.5 0 103 283 103 628.5 0 103 284 91 628.5 0 91 285 91 628.5 0 91 286 76 628.5 0 76 287 97 628.5 0 97 288 147 628.5 0 147 289 118 628.5 0 118 290 115 628.5 0 115 291 106 628.5 0 106 292 98 628.5 0 98 293 102 628.5 0 102 294 118 628.5 0 118 295 116 628.5 0 116 296 106 628.5 0 106 297 114 628.5 0 114 298 125 628.5 0 125 299 130 628.5 0 130 300 174 628.5 0 174 301 335 628.5 0 335 Finished in 24.97 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 607,706 Reads with adapters: 109,199 (18.0%) Reads that were too short: 348 (0.1%) Reads written (passing filters): 108,851 (17.9%) Total basepairs processed: 129,522,087 bp Total written (filtered): 29,608,524 bp (22.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 109199 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 17 0.0 3 0 0 0 6 11 18 65 0.0 3 0 0 33 22 10 19 1609 0.0 3 0 1090 218 198 103 20 104686 0.0 4 95179 7272 1623 463 149 21 301 0.0 4 47 215 17 11 11 22 37 0.0 4 6 5 20 4 2 23 1097 0.0 4 429 49 12 422 185 24 6 0.0 4 0 1 0 1 4 25 3 0.0 4 1 0 0 1 1 26 4 0.0 4 0 0 0 3 1 27 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 3 0.0 4 0 0 0 0 3 42 3 0.0 4 0 0 0 0 3 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 48 3 0.0 4 0 0 0 0 3 50 17 0.0 4 0 0 0 0 17 53 52 0.0 4 0 0 0 0 52 54 1 0.0 4 0 0 0 1 56 3 0.0 4 0 0 0 0 3 67 6 0.0 4 0 0 0 0 6 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 5 0.0 4 0 0 0 0 5 75 4 0.0 4 0 0 0 1 3 76 9 0.0 4 0 0 0 0 9 77 4 0.0 4 0 0 0 0 4 78 8 0.0 4 0 0 0 0 8 79 3 0.0 4 0 0 0 0 3 80 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 8 0.0 4 0 0 0 0 8 86 1 0.0 4 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 0 0 0 2 97 10 0.0 4 0 0 0 0 10 98 14 0.0 4 0 0 0 0 14 99 5 0.0 4 0 0 0 0 5 100 51 0.0 4 0 1 0 3 47 105 1 0.0 4 0 1 107 2 0.0 4 0 0 0 0 2 109 7 0.0 4 0 0 0 0 7 112 1 0.0 4 0 0 0 0 1 114 3 0.0 4 0 1 0 0 2 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 3 0.0 4 0 0 0 0 3 122 3 0.0 4 0 0 0 0 3 123 3 0.0 4 0 0 0 0 3 124 25 0.0 4 0 0 0 0 25 125 9 0.0 4 0 0 0 0 9 126 21 0.0 4 0 0 0 2 19 127 886 0.0 4 0 0 0 0 886 128 2 0.0 4 0 0 0 0 2 129 3 0.0 4 0 0 0 0 3 130 5 0.0 4 0 0 0 0 5 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 136 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 0 0 1 138 4 0.0 4 0 0 0 0 4 139 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 4 0.0 4 0 0 0 0 4 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 21 0.0 4 0 0 0 0 21 154 3 0.0 4 0 0 0 0 3 155 1 0.0 4 0 0 0 0 1 156 2 0.0 4 0 0 0 0 2 158 2 0.0 4 0 0 0 0 2 159 2 0.0 4 0 0 0 0 2 166 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 1 173 1 0.0 4 0 0 1 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 1 207 2 0.0 4 0 0 0 0 2 208 3 0.0 4 0 0 0 1 2 209 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 0 0 0 2 225 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 229 10 0.0 4 0 0 0 0 10 233 5 0.0 4 0 0 0 1 4 234 6 0.0 4 0 0 0 1 5 235 5 0.0 4 0 0 0 0 5 237 19 0.0 4 0 0 0 0 19 249 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 260 4 0.0 4 0 0 0 0 4 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 5 0.0 4 0 0 0 0 5 265 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 5 0.0 4 0 0 0 0 5 283 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 1 285 3 0.0 4 0 0 0 1 2 292 2 0.0 4 0 0 0 0 2 294 3 0.0 4 0 0 0 1 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.95 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 108,851 Reads with adapters: 17,676 (16.2%) Reads written (passing filters): 108,851 (100.0%) Total basepairs processed: 29,608,524 bp Total written (filtered): 29,357,153 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 17676 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 10.7% G: 81.7% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 311 1700.8 0 311 4 53 425.2 0 53 5 2 106.3 0 2 6 7 26.6 0 7 7 4 6.6 0 4 8 4 1.7 0 4 9 320 0.4 0 33 287 10 3033 0.1 1 302 2731 11 13308 0.0 1 560 12748 12 260 0.0 1 7 253 13 17 0.0 1 0 17 14 15 0.0 1 0 15 15 2 0.0 1 0 2 19 1 0.0 1 1 23 7 0.0 1 5 2 42 1 0.0 1 1 51 1 0.0 1 0 1 60 1 0.0 1 1 78 1 0.0 1 1 81 1 0.0 1 1 123 1 0.0 1 1 146 3 0.0 1 1 2 148 1 0.0 1 1 151 18 0.0 1 11 7 152 2 0.0 1 1 1 157 11 0.0 1 7 4 161 1 0.0 1 1 168 1 0.0 1 1 178 1 0.0 1 0 1 209 259 0.0 1 216 43 210 14 0.0 1 13 1 211 2 0.0 1 2 224 4 0.0 1 3 1 228 1 0.0 1 1 229 7 0.0 1 6 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.