This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.52 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 654,139 (25.4%) Reads that were too short: 14,216 (0.6%) Reads written (passing filters): 639,923 (24.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 136,319,804 bp (17.6%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 654139 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1861 628.5 0 1861 7 115126 628.5 0 115126 8 130048 628.5 0 130048 9 206 628.5 0 206 10 100 628.5 0 100 11 95 628.5 0 95 12 111 628.5 0 111 13 79 628.5 0 79 14 104 628.5 0 104 15 117 628.5 0 117 16 82 628.5 0 82 17 74 628.5 0 74 18 87 628.5 0 87 19 53 628.5 0 53 20 66 628.5 0 66 21 65 628.5 0 65 22 81 628.5 0 81 23 91 628.5 0 91 24 79 628.5 0 79 25 89 628.5 0 89 26 163 628.5 0 163 27 133 628.5 0 133 28 130 628.5 0 130 29 80 628.5 0 80 30 132 628.5 0 132 31 76 628.5 0 76 32 73 628.5 0 73 33 83 628.5 0 83 34 91 628.5 0 91 35 121 628.5 0 121 36 132 628.5 0 132 37 116 628.5 0 116 38 93 628.5 0 93 39 117 628.5 0 117 40 131 628.5 0 131 41 96 628.5 0 96 42 126 628.5 0 126 43 163 628.5 0 163 44 102 628.5 0 102 45 100 628.5 0 100 46 103 628.5 0 103 47 98 628.5 0 98 48 110 628.5 0 110 49 119 628.5 0 119 50 130 628.5 0 130 51 110 628.5 0 110 52 122 628.5 0 122 53 124 628.5 0 124 54 126 628.5 0 126 55 120 628.5 0 120 56 122 628.5 0 122 57 97 628.5 0 97 58 112 628.5 0 112 59 102 628.5 0 102 60 112 628.5 0 112 61 159 628.5 0 159 62 136 628.5 0 136 63 147 628.5 0 147 64 113 628.5 0 113 65 99 628.5 0 99 66 134 628.5 0 134 67 98 628.5 0 98 68 140 628.5 0 140 69 178 628.5 0 178 70 126 628.5 0 126 71 101 628.5 0 101 72 150 628.5 0 150 73 93 628.5 0 93 74 163 628.5 0 163 75 161 628.5 0 161 76 193 628.5 0 193 77 155 628.5 0 155 78 223 628.5 0 223 79 244 628.5 0 244 80 269 628.5 0 269 81 691 628.5 0 691 82 477 628.5 0 477 83 824 628.5 0 824 84 182 628.5 0 182 85 263 628.5 0 263 86 374 628.5 0 374 87 148 628.5 0 148 88 161 628.5 0 161 89 174 628.5 0 174 90 139 628.5 0 139 91 119 628.5 0 119 92 149 628.5 0 149 93 152 628.5 0 152 94 199 628.5 0 199 95 213 628.5 0 213 96 172 628.5 0 172 97 150 628.5 0 150 98 164 628.5 0 164 99 179 628.5 0 179 100 161 628.5 0 161 101 286 628.5 0 286 102 288 628.5 0 288 103 292 628.5 0 292 104 382 628.5 0 382 105 700 628.5 0 700 106 280 628.5 0 280 107 370 628.5 0 370 108 623 628.5 0 623 109 346 628.5 0 346 110 337 628.5 0 337 111 632 628.5 0 632 112 592 628.5 0 592 113 593 628.5 0 593 114 552 628.5 0 552 115 849 628.5 0 849 116 967 628.5 0 967 117 959 628.5 0 959 118 13266 628.5 0 13266 119 35016 628.5 0 35016 120 35404 628.5 0 35404 121 1578 628.5 0 1578 122 2289 628.5 0 2289 123 2890 628.5 0 2890 124 473 628.5 0 473 125 267 628.5 0 267 126 340 628.5 0 340 127 541 628.5 0 541 128 664 628.5 0 664 129 406 628.5 0 406 130 541 628.5 0 541 131 704 628.5 0 704 132 1319 628.5 0 1319 133 653 628.5 0 653 134 681 628.5 0 681 135 1001 628.5 0 1001 136 646 628.5 0 646 137 707 628.5 0 707 138 1138 628.5 0 1138 139 2849 628.5 0 2849 140 10948 628.5 0 10948 141 11531 628.5 0 11531 142 9591 628.5 0 9591 143 2168 628.5 0 2168 144 3588 628.5 0 3588 145 29128 628.5 0 29128 146 54966 628.5 0 54966 147 70597 628.5 0 70597 148 8094 628.5 0 8094 149 5161 628.5 0 5161 150 942 628.5 0 942 151 931 628.5 0 931 152 1015 628.5 0 1015 153 974 628.5 0 974 154 1133 628.5 0 1133 155 1055 628.5 0 1055 156 591 628.5 0 591 157 977 628.5 0 977 158 1261 628.5 0 1261 159 1285 628.5 0 1285 160 443 628.5 0 443 161 434 628.5 0 434 162 482 628.5 0 482 163 703 628.5 0 703 164 801 628.5 0 801 165 8413 628.5 0 8413 166 15220 628.5 0 15220 167 19275 628.5 0 19275 168 463 628.5 0 463 169 158 628.5 0 158 170 124 628.5 0 124 171 107 628.5 0 107 172 137 628.5 0 137 173 104 628.5 0 104 174 193 628.5 0 193 175 149 628.5 0 149 176 133 628.5 0 133 177 101 628.5 0 101 178 126 628.5 0 126 179 126 628.5 0 126 180 126 628.5 0 126 181 134 628.5 0 134 182 134 628.5 0 134 183 123 628.5 0 123 184 137 628.5 0 137 185 113 628.5 0 113 186 119 628.5 0 119 187 91 628.5 0 91 188 120 628.5 0 120 189 137 628.5 0 137 190 155 628.5 0 155 191 106 628.5 0 106 192 130 628.5 0 130 193 144 628.5 0 144 194 247 628.5 0 247 195 150 628.5 0 150 196 124 628.5 0 124 197 131 628.5 0 131 198 128 628.5 0 128 199 230 628.5 0 230 200 179 628.5 0 179 201 164 628.5 0 164 202 124 628.5 0 124 203 127 628.5 0 127 204 144 628.5 0 144 205 121 628.5 0 121 206 126 628.5 0 126 207 151 628.5 0 151 208 155 628.5 0 155 209 169 628.5 0 169 210 150 628.5 0 150 211 129 628.5 0 129 212 122 628.5 0 122 213 113 628.5 0 113 214 112 628.5 0 112 215 130 628.5 0 130 216 326 628.5 0 326 217 451 628.5 0 451 218 498 628.5 0 498 219 363 628.5 0 363 220 368 628.5 0 368 221 169 628.5 0 169 222 142 628.5 0 142 223 108 628.5 0 108 224 97 628.5 0 97 225 126 628.5 0 126 226 290 628.5 0 290 227 145 628.5 0 145 228 135 628.5 0 135 229 85 628.5 0 85 230 111 628.5 0 111 231 114 628.5 0 114 232 108 628.5 0 108 233 108 628.5 0 108 234 122 628.5 0 122 235 89 628.5 0 89 236 94 628.5 0 94 237 107 628.5 0 107 238 106 628.5 0 106 239 111 628.5 0 111 240 108 628.5 0 108 241 146 628.5 0 146 242 115 628.5 0 115 243 105 628.5 0 105 244 142 628.5 0 142 245 135 628.5 0 135 246 109 628.5 0 109 247 124 628.5 0 124 248 98 628.5 0 98 249 95 628.5 0 95 250 104 628.5 0 104 251 106 628.5 0 106 252 79 628.5 0 79 253 111 628.5 0 111 254 105 628.5 0 105 255 121 628.5 0 121 256 122 628.5 0 122 257 138 628.5 0 138 258 127 628.5 0 127 259 114 628.5 0 114 260 136 628.5 0 136 261 116 628.5 0 116 262 136 628.5 0 136 263 136 628.5 0 136 264 127 628.5 0 127 265 140 628.5 0 140 266 279 628.5 0 279 267 141 628.5 0 141 268 104 628.5 0 104 269 95 628.5 0 95 270 111 628.5 0 111 271 104 628.5 0 104 272 93 628.5 0 93 273 104 628.5 0 104 274 103 628.5 0 103 275 120 628.5 0 120 276 112 628.5 0 112 277 100 628.5 0 100 278 129 628.5 0 129 279 134 628.5 0 134 280 105 628.5 0 105 281 137 628.5 0 137 282 138 628.5 0 138 283 100 628.5 0 100 284 116 628.5 0 116 285 128 628.5 0 128 286 99 628.5 0 99 287 117 628.5 0 117 288 143 628.5 0 143 289 125 628.5 0 125 290 152 628.5 0 152 291 132 628.5 0 132 292 118 628.5 0 118 293 122 628.5 0 122 294 138 628.5 0 138 295 148 628.5 0 148 296 130 628.5 0 130 297 163 628.5 0 163 298 121 628.5 0 121 299 163 628.5 0 163 300 168 628.5 0 168 301 383 628.5 0 383 Finished in 22.96 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 639,923 Reads with adapters: 117,069 (18.3%) Reads that were too short: 377 (0.1%) Reads written (passing filters): 116,692 (18.2%) Total basepairs processed: 136,319,804 bp Total written (filtered): 31,885,977 bp (23.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 117069 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 10 16 545 0.0 3 0 535 9 1 17 113029 0.0 3 110881 2085 53 10 18 217 0.0 3 20 187 9 1 19 27 0.0 3 1 0 23 3 20 114 0.0 3 2 0 0 112 21 1 0.0 3 0 0 0 1 23 18 0.0 3 0 0 0 18 25 3 0.0 3 1 0 0 2 26 1121 0.0 3 0 0 8 1113 27 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 29 147 0.0 3 0 0 2 145 30 4 0.0 3 1 0 0 3 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 1 42 5 0.0 3 0 0 0 5 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 6 0.0 3 0 0 0 6 48 4 0.0 3 0 0 0 4 49 1 0.0 3 0 0 0 1 50 45 0.0 3 0 0 1 44 51 1 0.0 3 0 0 0 1 52 52 0.0 3 0 0 0 52 53 53 0.0 3 0 0 0 53 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 72 34 0.0 3 0 0 0 34 73 109 0.0 3 0 0 0 109 74 1050 0.0 3 0 0 5 1045 75 1 0.0 3 0 1 76 9 0.0 3 0 0 0 9 77 65 0.0 3 0 0 1 64 78 1 0.0 3 1 79 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 1 0.0 3 1 85 2 0.0 3 0 0 0 2 86 5 0.0 3 0 0 0 5 87 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 0 4 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 4 0.0 3 0 0 0 4 110 2 0.0 3 0 0 0 2 111 5 0.0 3 0 0 0 5 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 9 0.0 3 0 1 0 8 126 2 0.0 3 0 0 0 2 127 5 0.0 3 0 0 0 5 128 23 0.0 3 0 0 1 22 129 9 0.0 3 0 0 0 9 133 13 0.0 3 0 0 0 13 134 3 0.0 3 0 0 0 3 135 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 138 64 0.0 3 0 0 0 64 139 7 0.0 3 0 0 0 7 140 10 0.0 3 0 0 0 10 141 40 0.0 3 0 0 2 38 146 5 0.0 3 0 0 0 5 147 5 0.0 3 0 0 0 5 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 25 0.0 3 0 0 2 23 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 165 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 167 1 0.0 3 1 171 1 0.0 3 0 0 0 1 173 2 0.0 3 0 1 1 174 4 0.0 3 0 1 0 3 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 44 0.0 3 0 0 0 44 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 230 3 0.0 3 0 0 0 3 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 256 1 0.0 3 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.06 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 116,692 Reads with adapters: 146 (0.1%) Reads written (passing filters): 116,692 (100.0%) Total basepairs processed: 31,885,977 bp Total written (filtered): 31,885,488 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 146 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.0% C: 21.9% G: 29.5% T: 11.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 107 1823.3 0 107 4 29 455.8 0 29 5 8 114.0 0 8 6 2 28.5 0 2