This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.66 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 128,392 (5.0%) Reads that were too short: 24,809 (1.0%) Reads written (passing filters): 103,583 (4.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 25,185,949 bp (3.3%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 128392 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1664 628.5 0 1664 7 27426 628.5 0 27426 8 2904 628.5 0 2904 9 29907 628.5 0 29907 10 56 628.5 0 56 11 59 628.5 0 59 12 49 628.5 0 49 13 41 628.5 0 41 14 38 628.5 0 38 15 50 628.5 0 50 16 54 628.5 0 54 17 40 628.5 0 40 18 34 628.5 0 34 19 64 628.5 0 64 20 32 628.5 0 32 21 49 628.5 0 49 22 51 628.5 0 51 23 39 628.5 0 39 24 53 628.5 0 53 25 53 628.5 0 53 26 42 628.5 0 42 27 44 628.5 0 44 28 83 628.5 0 83 29 64 628.5 0 64 30 53 628.5 0 53 31 78 628.5 0 78 32 59 628.5 0 59 33 484 628.5 0 484 34 345 628.5 0 345 35 1172 628.5 0 1172 36 35 628.5 0 35 37 30 628.5 0 30 38 39 628.5 0 39 39 45 628.5 0 45 40 35 628.5 0 35 41 35 628.5 0 35 42 35 628.5 0 35 43 43 628.5 0 43 44 46 628.5 0 46 45 87 628.5 0 87 46 68 628.5 0 68 47 81 628.5 0 81 48 50 628.5 0 50 49 63 628.5 0 63 50 59 628.5 0 59 51 51 628.5 0 51 52 28 628.5 0 28 53 50 628.5 0 50 54 54 628.5 0 54 55 53 628.5 0 53 56 75 628.5 0 75 57 362 628.5 0 362 58 501 628.5 0 501 59 254 628.5 0 254 60 65 628.5 0 65 61 35 628.5 0 35 62 59 628.5 0 59 63 51 628.5 0 51 64 75 628.5 0 75 65 46 628.5 0 46 66 50 628.5 0 50 67 47 628.5 0 47 68 105 628.5 0 105 69 63 628.5 0 63 70 56 628.5 0 56 71 41 628.5 0 41 72 168 628.5 0 168 73 105 628.5 0 105 74 322 628.5 0 322 75 49 628.5 0 49 76 59 628.5 0 59 77 77 628.5 0 77 78 66 628.5 0 66 79 68 628.5 0 68 80 32 628.5 0 32 81 49 628.5 0 49 82 64 628.5 0 64 83 43 628.5 0 43 84 158 628.5 0 158 85 178 628.5 0 178 86 224 628.5 0 224 87 55 628.5 0 55 88 63 628.5 0 63 89 50 628.5 0 50 90 76 628.5 0 76 91 76 628.5 0 76 92 122 628.5 0 122 93 62 628.5 0 62 94 77 628.5 0 77 95 59 628.5 0 59 96 68 628.5 0 68 97 85 628.5 0 85 98 78 628.5 0 78 99 96 628.5 0 96 100 47 628.5 0 47 101 38 628.5 0 38 102 61 628.5 0 61 103 42 628.5 0 42 104 58 628.5 0 58 105 82 628.5 0 82 106 93 628.5 0 93 107 671 628.5 0 671 108 1109 628.5 0 1109 109 1383 628.5 0 1383 110 97 628.5 0 97 111 50 628.5 0 50 112 48 628.5 0 48 113 40 628.5 0 40 114 56 628.5 0 56 115 54 628.5 0 54 116 50 628.5 0 50 117 58 628.5 0 58 118 41 628.5 0 41 119 48 628.5 0 48 120 74 628.5 0 74 121 145 628.5 0 145 122 92 628.5 0 92 123 121 628.5 0 121 124 69 628.5 0 69 125 65 628.5 0 65 126 111 628.5 0 111 127 846 628.5 0 846 128 1090 628.5 0 1090 129 821 628.5 0 821 130 145 628.5 0 145 131 92 628.5 0 92 132 73 628.5 0 73 133 70 628.5 0 70 134 69 628.5 0 69 135 110 628.5 0 110 136 276 628.5 0 276 137 144 628.5 0 144 138 202 628.5 0 202 139 81 628.5 0 81 140 184 628.5 0 184 141 262 628.5 0 262 142 360 628.5 0 360 143 2322 628.5 0 2322 144 1819 628.5 0 1819 145 2320 628.5 0 2320 146 245 628.5 0 245 147 457 628.5 0 457 148 71 628.5 0 71 149 125 628.5 0 125 150 130 628.5 0 130 151 110 628.5 0 110 152 233 628.5 0 233 153 252 628.5 0 252 154 1634 628.5 0 1634 155 1541 628.5 0 1541 156 1060 628.5 0 1060 157 98 628.5 0 98 158 64 628.5 0 64 159 74 628.5 0 74 160 91 628.5 0 91 161 107 628.5 0 107 162 144 628.5 0 144 163 65 628.5 0 65 164 55 628.5 0 55 165 59 628.5 0 59 166 83 628.5 0 83 167 124 628.5 0 124 168 62 628.5 0 62 169 119 628.5 0 119 170 76 628.5 0 76 171 117 628.5 0 117 172 72 628.5 0 72 173 89 628.5 0 89 174 87 628.5 0 87 175 216 628.5 0 216 176 123 628.5 0 123 177 93 628.5 0 93 178 63 628.5 0 63 179 75 628.5 0 75 180 70 628.5 0 70 181 114 628.5 0 114 182 618 628.5 0 618 183 169 628.5 0 169 184 189 628.5 0 189 185 269 628.5 0 269 186 2629 628.5 0 2629 187 1351 628.5 0 1351 188 2746 628.5 0 2746 189 63 628.5 0 63 190 47 628.5 0 47 191 55 628.5 0 55 192 52 628.5 0 52 193 50 628.5 0 50 194 46 628.5 0 46 195 79 628.5 0 79 196 58 628.5 0 58 197 56 628.5 0 56 198 48 628.5 0 48 199 61 628.5 0 61 200 66 628.5 0 66 201 56 628.5 0 56 202 102 628.5 0 102 203 102 628.5 0 102 204 122 628.5 0 122 205 64 628.5 0 64 206 71 628.5 0 71 207 67 628.5 0 67 208 151 628.5 0 151 209 130 628.5 0 130 210 143 628.5 0 143 211 91 628.5 0 91 212 72 628.5 0 72 213 59 628.5 0 59 214 52 628.5 0 52 215 77 628.5 0 77 216 144 628.5 0 144 217 719 628.5 0 719 218 443 628.5 0 443 219 508 628.5 0 508 220 56 628.5 0 56 221 73 628.5 0 73 222 170 628.5 0 170 223 101 628.5 0 101 224 95 628.5 0 95 225 63 628.5 0 63 226 82 628.5 0 82 227 79 628.5 0 79 228 120 628.5 0 120 229 63 628.5 0 63 230 77 628.5 0 77 231 93 628.5 0 93 232 107 628.5 0 107 233 103 628.5 0 103 234 102 628.5 0 102 235 98 628.5 0 98 236 94 628.5 0 94 237 117 628.5 0 117 238 112 628.5 0 112 239 114 628.5 0 114 240 80 628.5 0 80 241 147 628.5 0 147 242 227 628.5 0 227 243 355 628.5 0 355 244 219 628.5 0 219 245 314 628.5 0 314 246 167 628.5 0 167 247 122 628.5 0 122 248 119 628.5 0 119 249 80 628.5 0 80 250 597 628.5 0 597 251 731 628.5 0 731 252 1372 628.5 0 1372 253 145 628.5 0 145 254 268 628.5 0 268 255 163 628.5 0 163 256 96 628.5 0 96 257 97 628.5 0 97 258 89 628.5 0 89 259 78 628.5 0 78 260 122 628.5 0 122 261 316 628.5 0 316 262 339 628.5 0 339 263 592 628.5 0 592 264 119 628.5 0 119 265 75 628.5 0 75 266 135 628.5 0 135 267 133 628.5 0 133 268 102 628.5 0 102 269 101 628.5 0 101 270 171 628.5 0 171 271 316 628.5 0 316 272 278 628.5 0 278 273 173 628.5 0 173 274 102 628.5 0 102 275 118 628.5 0 118 276 114 628.5 0 114 277 145 628.5 0 145 278 274 628.5 0 274 279 357 628.5 0 357 280 2842 628.5 0 2842 281 567 628.5 0 567 282 971 628.5 0 971 283 231 628.5 0 231 284 111 628.5 0 111 285 196 628.5 0 196 286 124 628.5 0 124 287 119 628.5 0 119 288 89 628.5 0 89 289 130 628.5 0 130 290 213 628.5 0 213 291 233 628.5 0 233 292 150 628.5 0 150 293 151 628.5 0 151 294 210 628.5 0 210 295 215 628.5 0 215 296 418 628.5 0 418 297 1387 628.5 0 1387 298 807 628.5 0 807 299 760 628.5 0 760 300 213 628.5 0 213 301 88 628.5 0 88 Finished in 5.04 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 103,583 Reads with adapters: 28,909 (27.9%) Reads that were too short: 76 (0.1%) Reads written (passing filters): 28,833 (27.8%) Total basepairs processed: 25,185,949 bp Total written (filtered): 7,832,910 bp (31.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28909 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 17 0.0 3 0 0 13 4 19 372 0.0 3 0 301 37 24 10 20 27660 0.0 4 25618 1597 322 91 32 21 77 0.0 4 10 62 2 1 2 22 9 0.0 4 0 0 8 1 23 64 0.0 4 1 1 1 43 18 24 1 0.0 4 0 0 0 0 1 25 12 0.0 4 0 0 0 0 12 26 2 0.0 4 0 0 0 1 1 27 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 1 41 1 0.0 4 0 0 0 0 1 44 4 0.0 4 0 0 0 0 4 45 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 2 0.0 4 1 0 0 0 1 66 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 16 0.0 4 0 0 0 0 16 127 567 0.0 4 0 0 0 0 567 128 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 3 0.0 4 0 0 0 0 3 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 1 175 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 1 6 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 238 25 0.0 4 0 0 0 2 23 253 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.01 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 28,833 Reads with adapters: 1,569 (5.4%) Reads written (passing filters): 28,833 (100.0%) Total basepairs processed: 7,832,910 bp Total written (filtered): 7,814,344 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1569 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.3% C: 15.9% G: 70.6% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 186 450.5 0 186 4 29 112.6 0 29 5 3 28.2 0 3 8 1 0.4 0 1 9 13 0.1 0 0 13 10 39 0.0 1 0 39 11 857 0.0 1 57 800 12 395 0.0 1 14 381 13 27 0.0 1 0 27 15 3 0.0 1 0 3 16 1 0.0 1 0 1 94 1 0.0 1 1 152 4 0.0 1 3 1 191 1 0.0 1 0 1 210 9 0.0 1 8 1