This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.60 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 114,504 (4.4%) Reads that were too short: 11,977 (0.5%) Reads written (passing filters): 102,527 (4.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 25,191,637 bp (3.3%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 114504 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1592 628.5 0 1592 7 34552 628.5 0 34552 8 1964 628.5 0 1964 9 27444 628.5 0 27444 10 54 628.5 0 54 11 43 628.5 0 43 12 47 628.5 0 47 13 21 628.5 0 21 14 39 628.5 0 39 15 25 628.5 0 25 16 50 628.5 0 50 17 39 628.5 0 39 18 34 628.5 0 34 19 45 628.5 0 45 20 35 628.5 0 35 21 42 628.5 0 42 22 53 628.5 0 53 23 33 628.5 0 33 24 32 628.5 0 32 25 35 628.5 0 35 26 34 628.5 0 34 27 47 628.5 0 47 28 35 628.5 0 35 29 45 628.5 0 45 30 85 628.5 0 85 31 55 628.5 0 55 32 46 628.5 0 46 33 402 628.5 0 402 34 212 628.5 0 212 35 396 628.5 0 396 36 41 628.5 0 41 37 33 628.5 0 33 38 53 628.5 0 53 39 42 628.5 0 42 40 26 628.5 0 26 41 47 628.5 0 47 42 33 628.5 0 33 43 60 628.5 0 60 44 39 628.5 0 39 45 57 628.5 0 57 46 66 628.5 0 66 47 61 628.5 0 61 48 44 628.5 0 44 49 48 628.5 0 48 50 57 628.5 0 57 51 51 628.5 0 51 52 38 628.5 0 38 53 56 628.5 0 56 54 33 628.5 0 33 55 38 628.5 0 38 56 56 628.5 0 56 57 101 628.5 0 101 58 102 628.5 0 102 59 64 628.5 0 64 60 68 628.5 0 68 61 38 628.5 0 38 62 53 628.5 0 53 63 37 628.5 0 37 64 53 628.5 0 53 65 48 628.5 0 48 66 50 628.5 0 50 67 41 628.5 0 41 68 58 628.5 0 58 69 40 628.5 0 40 70 58 628.5 0 58 71 40 628.5 0 40 72 201 628.5 0 201 73 72 628.5 0 72 74 340 628.5 0 340 75 38 628.5 0 38 76 42 628.5 0 42 77 60 628.5 0 60 78 51 628.5 0 51 79 43 628.5 0 43 80 36 628.5 0 36 81 35 628.5 0 35 82 50 628.5 0 50 83 32 628.5 0 32 84 106 628.5 0 106 85 128 628.5 0 128 86 217 628.5 0 217 87 41 628.5 0 41 88 34 628.5 0 34 89 42 628.5 0 42 90 45 628.5 0 45 91 38 628.5 0 38 92 36 628.5 0 36 93 24 628.5 0 24 94 61 628.5 0 61 95 36 628.5 0 36 96 43 628.5 0 43 97 37 628.5 0 37 98 39 628.5 0 39 99 61 628.5 0 61 100 44 628.5 0 44 101 55 628.5 0 55 102 56 628.5 0 56 103 43 628.5 0 43 104 45 628.5 0 45 105 53 628.5 0 53 106 53 628.5 0 53 107 419 628.5 0 419 108 751 628.5 0 751 109 933 628.5 0 933 110 52 628.5 0 52 111 48 628.5 0 48 112 55 628.5 0 55 113 38 628.5 0 38 114 58 628.5 0 58 115 39 628.5 0 39 116 42 628.5 0 42 117 58 628.5 0 58 118 35 628.5 0 35 119 39 628.5 0 39 120 59 628.5 0 59 121 55 628.5 0 55 122 49 628.5 0 49 123 56 628.5 0 56 124 57 628.5 0 57 125 82 628.5 0 82 126 96 628.5 0 96 127 994 628.5 0 994 128 1400 628.5 0 1400 129 952 628.5 0 952 130 89 628.5 0 89 131 58 628.5 0 58 132 68 628.5 0 68 133 75 628.5 0 75 134 51 628.5 0 51 135 56 628.5 0 56 136 119 628.5 0 119 137 100 628.5 0 100 138 119 628.5 0 119 139 53 628.5 0 53 140 85 628.5 0 85 141 127 628.5 0 127 142 225 628.5 0 225 143 2654 628.5 0 2654 144 2044 628.5 0 2044 145 2844 628.5 0 2844 146 74 628.5 0 74 147 127 628.5 0 127 148 55 628.5 0 55 149 69 628.5 0 69 150 55 628.5 0 55 151 70 628.5 0 70 152 88 628.5 0 88 153 190 628.5 0 190 154 1330 628.5 0 1330 155 1392 628.5 0 1392 156 258 628.5 0 258 157 55 628.5 0 55 158 42 628.5 0 42 159 44 628.5 0 44 160 64 628.5 0 64 161 89 628.5 0 89 162 100 628.5 0 100 163 57 628.5 0 57 164 55 628.5 0 55 165 49 628.5 0 49 166 67 628.5 0 67 167 64 628.5 0 64 168 59 628.5 0 59 169 73 628.5 0 73 170 64 628.5 0 64 171 92 628.5 0 92 172 64 628.5 0 64 173 60 628.5 0 60 174 61 628.5 0 61 175 86 628.5 0 86 176 72 628.5 0 72 177 48 628.5 0 48 178 60 628.5 0 60 179 56 628.5 0 56 180 72 628.5 0 72 181 100 628.5 0 100 182 652 628.5 0 652 183 171 628.5 0 171 184 196 628.5 0 196 185 221 628.5 0 221 186 3372 628.5 0 3372 187 1525 628.5 0 1525 188 3614 628.5 0 3614 189 51 628.5 0 51 190 48 628.5 0 48 191 45 628.5 0 45 192 39 628.5 0 39 193 37 628.5 0 37 194 35 628.5 0 35 195 43 628.5 0 43 196 42 628.5 0 42 197 37 628.5 0 37 198 50 628.5 0 50 199 47 628.5 0 47 200 50 628.5 0 50 201 54 628.5 0 54 202 50 628.5 0 50 203 29 628.5 0 29 204 68 628.5 0 68 205 51 628.5 0 51 206 68 628.5 0 68 207 47 628.5 0 47 208 53 628.5 0 53 209 65 628.5 0 65 210 112 628.5 0 112 211 53 628.5 0 53 212 66 628.5 0 66 213 72 628.5 0 72 214 51 628.5 0 51 215 59 628.5 0 59 216 111 628.5 0 111 217 846 628.5 0 846 218 456 628.5 0 456 219 634 628.5 0 634 220 47 628.5 0 47 221 45 628.5 0 45 222 133 628.5 0 133 223 49 628.5 0 49 224 70 628.5 0 70 225 39 628.5 0 39 226 34 628.5 0 34 227 45 628.5 0 45 228 65 628.5 0 65 229 49 628.5 0 49 230 44 628.5 0 44 231 25 628.5 0 25 232 66 628.5 0 66 233 59 628.5 0 59 234 40 628.5 0 40 235 56 628.5 0 56 236 46 628.5 0 46 237 61 628.5 0 61 238 53 628.5 0 53 239 74 628.5 0 74 240 38 628.5 0 38 241 64 628.5 0 64 242 70 628.5 0 70 243 93 628.5 0 93 244 54 628.5 0 54 245 78 628.5 0 78 246 50 628.5 0 50 247 71 628.5 0 71 248 59 628.5 0 59 249 44 628.5 0 44 250 249 628.5 0 249 251 385 628.5 0 385 252 609 628.5 0 609 253 80 628.5 0 80 254 83 628.5 0 83 255 65 628.5 0 65 256 44 628.5 0 44 257 47 628.5 0 47 258 62 628.5 0 62 259 48 628.5 0 48 260 49 628.5 0 49 261 111 628.5 0 111 262 152 628.5 0 152 263 262 628.5 0 262 264 95 628.5 0 95 265 50 628.5 0 50 266 78 628.5 0 78 267 77 628.5 0 77 268 75 628.5 0 75 269 81 628.5 0 81 270 71 628.5 0 71 271 130 628.5 0 130 272 107 628.5 0 107 273 61 628.5 0 61 274 50 628.5 0 50 275 72 628.5 0 72 276 68 628.5 0 68 277 57 628.5 0 57 278 183 628.5 0 183 279 141 628.5 0 141 280 1015 628.5 0 1015 281 199 628.5 0 199 282 341 628.5 0 341 283 75 628.5 0 75 284 55 628.5 0 55 285 86 628.5 0 86 286 86 628.5 0 86 287 65 628.5 0 65 288 66 628.5 0 66 289 63 628.5 0 63 290 100 628.5 0 100 291 118 628.5 0 118 292 53 628.5 0 53 293 68 628.5 0 68 294 102 628.5 0 102 295 113 628.5 0 113 296 129 628.5 0 129 297 377 628.5 0 377 298 199 628.5 0 199 299 190 628.5 0 190 300 186 628.5 0 186 301 37 628.5 0 37 Finished in 4.53 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 102,527 Reads with adapters: 30,069 (29.3%) Reads that were too short: 143 (0.1%) Reads written (passing filters): 29,926 (29.2%) Total basepairs processed: 25,191,637 bp Total written (filtered): 8,210,976 bp (32.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30069 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 8 0.0 3 0 0 8 16 185 0.0 3 0 180 5 17 29443 0.0 3 28864 566 12 1 18 42 0.0 3 1 36 5 19 23 0.0 3 0 0 22 1 20 84 0.0 3 62 1 0 21 21 2 0.0 3 0 0 1 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 36 3 0.0 3 1 0 0 2 37 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 28 0.0 3 0 0 0 28 51 2 0.0 3 0 0 0 2 54 1 0.0 3 1 56 1 0.0 3 0 1 59 17 0.0 3 0 0 0 17 60 5 0.0 3 0 0 0 5 61 14 0.0 3 0 0 0 14 62 1 0.0 3 0 0 0 1 64 5 0.0 3 0 0 0 5 65 1 0.0 3 0 0 0 1 66 5 0.0 3 0 0 0 5 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 13 0.0 3 0 0 1 12 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 66 0.0 3 0 0 0 66 134 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 2 0.0 3 0 1 0 1 150 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 61 0.0 3 0 0 0 61 276 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.07 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 29,926 Reads with adapters: 1,283 (4.3%) Reads written (passing filters): 29,926 (100.0%) Total basepairs processed: 8,210,976 bp Total written (filtered): 8,207,081 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1283 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.4% C: 6.5% G: 11.4% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1246 467.6 0 1246 4 35 116.9 0 35 5 1 29.2 0 1 12 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.