This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.63 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 271,135 (10.5%) Reads that were too short: 11,217 (0.4%) Reads written (passing filters): 259,918 (10.1%) Total basepairs processed: 774,927,510 bp Total written (filtered): 73,007,548 bp (9.4%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 271135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3444 628.5 0 3444 7 7094 628.5 0 7094 8 119685 628.5 0 119685 9 103498 628.5 0 103498 10 167 628.5 0 167 11 12 628.5 0 12 12 30 628.5 0 30 13 21 628.5 0 21 14 16 628.5 0 16 15 13 628.5 0 13 16 20 628.5 0 20 17 40 628.5 0 40 18 21 628.5 0 21 19 29 628.5 0 29 20 22 628.5 0 22 21 10 628.5 0 10 22 17 628.5 0 17 23 17 628.5 0 17 24 14 628.5 0 14 25 19 628.5 0 19 26 16 628.5 0 16 27 16 628.5 0 16 28 23 628.5 0 23 29 14 628.5 0 14 30 28 628.5 0 28 31 18 628.5 0 18 32 22 628.5 0 22 33 18 628.5 0 18 34 21 628.5 0 21 35 24 628.5 0 24 36 14 628.5 0 14 37 16 628.5 0 16 38 14 628.5 0 14 39 19 628.5 0 19 40 36 628.5 0 36 41 18 628.5 0 18 42 34 628.5 0 34 43 25 628.5 0 25 44 29 628.5 0 29 45 35 628.5 0 35 46 32 628.5 0 32 47 89 628.5 0 89 48 49 628.5 0 49 49 165 628.5 0 165 50 69 628.5 0 69 51 11 628.5 0 11 52 26 628.5 0 26 53 26 628.5 0 26 54 21 628.5 0 21 55 22 628.5 0 22 56 22 628.5 0 22 57 12 628.5 0 12 58 58 628.5 0 58 59 70 628.5 0 70 60 58 628.5 0 58 61 26 628.5 0 26 62 33 628.5 0 33 63 20 628.5 0 20 64 23 628.5 0 23 65 12 628.5 0 12 66 165 628.5 0 165 67 133 628.5 0 133 68 246 628.5 0 246 69 23 628.5 0 23 70 42 628.5 0 42 71 48 628.5 0 48 72 19 628.5 0 19 73 30 628.5 0 30 74 19 628.5 0 19 75 24 628.5 0 24 76 33 628.5 0 33 77 32 628.5 0 32 78 26 628.5 0 26 79 24 628.5 0 24 80 18 628.5 0 18 81 28 628.5 0 28 82 50 628.5 0 50 83 39 628.5 0 39 84 62 628.5 0 62 85 86 628.5 0 86 86 46 628.5 0 46 87 33 628.5 0 33 88 20 628.5 0 20 89 57 628.5 0 57 90 26 628.5 0 26 91 57 628.5 0 57 92 47 628.5 0 47 93 60 628.5 0 60 94 41 628.5 0 41 95 35 628.5 0 35 96 23 628.5 0 23 97 33 628.5 0 33 98 30 628.5 0 30 99 37 628.5 0 37 100 43 628.5 0 43 101 27 628.5 0 27 102 33 628.5 0 33 103 63 628.5 0 63 104 93 628.5 0 93 105 35 628.5 0 35 106 46 628.5 0 46 107 68 628.5 0 68 108 48 628.5 0 48 109 56 628.5 0 56 110 65 628.5 0 65 111 242 628.5 0 242 112 551 628.5 0 551 113 231 628.5 0 231 114 156 628.5 0 156 115 824 628.5 0 824 116 505 628.5 0 505 117 2282 628.5 0 2282 118 2527 628.5 0 2527 119 4996 628.5 0 4996 120 72 628.5 0 72 121 45 628.5 0 45 122 43 628.5 0 43 123 22 628.5 0 22 124 47 628.5 0 47 125 65 628.5 0 65 126 39 628.5 0 39 127 63 628.5 0 63 128 55 628.5 0 55 129 55 628.5 0 55 130 54 628.5 0 54 131 54 628.5 0 54 132 105 628.5 0 105 133 105 628.5 0 105 134 41 628.5 0 41 135 48 628.5 0 48 136 30 628.5 0 30 137 38 628.5 0 38 138 46 628.5 0 46 139 44 628.5 0 44 140 50 628.5 0 50 141 48 628.5 0 48 142 38 628.5 0 38 143 50 628.5 0 50 144 81 628.5 0 81 145 70 628.5 0 70 146 149 628.5 0 149 147 114 628.5 0 114 148 143 628.5 0 143 149 105 628.5 0 105 150 97 628.5 0 97 151 856 628.5 0 856 152 796 628.5 0 796 153 1099 628.5 0 1099 154 1116 628.5 0 1116 155 39 628.5 0 39 156 67 628.5 0 67 157 50 628.5 0 50 158 27 628.5 0 27 159 44 628.5 0 44 160 74 628.5 0 74 161 61 628.5 0 61 162 223 628.5 0 223 163 391 628.5 0 391 164 700 628.5 0 700 165 35 628.5 0 35 166 53 628.5 0 53 167 32 628.5 0 32 168 32 628.5 0 32 169 48 628.5 0 48 170 26 628.5 0 26 171 39 628.5 0 39 172 45 628.5 0 45 173 41 628.5 0 41 174 31 628.5 0 31 175 36 628.5 0 36 176 36 628.5 0 36 177 28 628.5 0 28 178 48 628.5 0 48 179 259 628.5 0 259 180 130 628.5 0 130 181 206 628.5 0 206 182 111 628.5 0 111 183 225 628.5 0 225 184 55 628.5 0 55 185 79 628.5 0 79 186 22 628.5 0 22 187 42 628.5 0 42 188 32 628.5 0 32 189 51 628.5 0 51 190 55 628.5 0 55 191 69 628.5 0 69 192 69 628.5 0 69 193 47 628.5 0 47 194 28 628.5 0 28 195 32 628.5 0 32 196 33 628.5 0 33 197 45 628.5 0 45 198 55 628.5 0 55 199 46 628.5 0 46 200 42 628.5 0 42 201 40 628.5 0 40 202 35 628.5 0 35 203 53 628.5 0 53 204 47 628.5 0 47 205 47 628.5 0 47 206 40 628.5 0 40 207 39 628.5 0 39 208 46 628.5 0 46 209 35 628.5 0 35 210 53 628.5 0 53 211 58 628.5 0 58 212 57 628.5 0 57 213 55 628.5 0 55 214 85 628.5 0 85 215 40 628.5 0 40 216 66 628.5 0 66 217 130 628.5 0 130 218 253 628.5 0 253 219 236 628.5 0 236 220 247 628.5 0 247 221 88 628.5 0 88 222 34 628.5 0 34 223 57 628.5 0 57 224 66 628.5 0 66 225 80 628.5 0 80 226 74 628.5 0 74 227 86 628.5 0 86 228 91 628.5 0 91 229 94 628.5 0 94 230 108 628.5 0 108 231 286 628.5 0 286 232 234 628.5 0 234 233 489 628.5 0 489 234 209 628.5 0 209 235 182 628.5 0 182 236 224 628.5 0 224 237 179 628.5 0 179 238 122 628.5 0 122 239 131 628.5 0 131 240 96 628.5 0 96 241 44 628.5 0 44 242 60 628.5 0 60 243 61 628.5 0 61 244 89 628.5 0 89 245 160 628.5 0 160 246 157 628.5 0 157 247 276 628.5 0 276 248 118 628.5 0 118 249 98 628.5 0 98 250 81 628.5 0 81 251 96 628.5 0 96 252 132 628.5 0 132 253 128 628.5 0 128 254 184 628.5 0 184 255 133 628.5 0 133 256 111 628.5 0 111 257 101 628.5 0 101 258 66 628.5 0 66 259 62 628.5 0 62 260 48 628.5 0 48 261 48 628.5 0 48 262 36 628.5 0 36 263 71 628.5 0 71 264 79 628.5 0 79 265 111 628.5 0 111 266 101 628.5 0 101 267 107 628.5 0 107 268 92 628.5 0 92 269 45 628.5 0 45 270 56 628.5 0 56 271 78 628.5 0 78 272 126 628.5 0 126 273 114 628.5 0 114 274 55 628.5 0 55 275 57 628.5 0 57 276 45 628.5 0 45 277 70 628.5 0 70 278 124 628.5 0 124 279 118 628.5 0 118 280 114 628.5 0 114 281 60 628.5 0 60 282 120 628.5 0 120 283 162 628.5 0 162 284 249 628.5 0 249 285 175 628.5 0 175 286 100 628.5 0 100 287 95 628.5 0 95 288 102 628.5 0 102 289 82 628.5 0 82 290 74 628.5 0 74 291 151 628.5 0 151 292 130 628.5 0 130 293 182 628.5 0 182 294 104 628.5 0 104 295 96 628.5 0 96 296 81 628.5 0 81 297 124 628.5 0 124 298 141 628.5 0 141 299 253 628.5 0 253 300 163 628.5 0 163 301 169 628.5 0 169 Finished in 12.78 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 259,918 Reads with adapters: 111,455 (42.9%) Reads that were too short: 155 (0.1%) Reads written (passing filters): 111,300 (42.8%) Total basepairs processed: 73,007,548 bp Total written (filtered): 30,245,750 bp (41.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 111455 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 34 0.0 3 0 0 0 14 20 18 118 0.0 3 0 0 54 32 32 19 1394 0.0 3 0 1014 141 167 72 20 106348 0.0 4 95303 8273 1947 622 203 21 2239 0.0 4 1851 306 54 15 13 22 31 0.0 4 2 3 15 1 10 23 773 0.0 4 7 2 2 485 277 24 10 0.0 4 0 0 0 3 7 26 2 0.0 4 1 0 0 1 27 1 0.0 4 0 1 28 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 51 1 0.0 4 1 61 1 0.0 4 0 0 0 0 1 64 4 0.0 4 0 0 0 0 4 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 3 0.0 4 0 0 0 0 3 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 1 105 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 127 172 0.0 4 0 0 0 0 172 128 130 0.0 4 0 0 0 0 130 129 5 0.0 4 0 0 0 0 5 130 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 139 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 17 0.0 4 0 0 0 0 17 154 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 1 1 208 5 0.0 4 0 0 0 0 5 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 5 0.0 4 0 0 0 0 5 215 2 0.0 4 0 0 0 0 2 219 2 0.0 4 0 0 0 0 2 220 3 0.0 4 0 0 0 0 3 221 3 0.0 4 0 0 0 0 3 222 1 0.0 4 0 0 0 1 223 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 228 10 0.0 4 0 0 0 0 10 229 15 0.0 4 0 0 0 1 14 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 4 0.0 4 0 0 0 0 4 234 8 0.0 4 0 0 0 0 8 235 3 0.0 4 0 0 0 1 2 236 4 0.0 4 0 0 0 0 4 237 25 0.0 4 0 0 0 2 23 238 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 262 6 0.0 4 0 0 0 1 5 263 5 0.0 4 0 0 0 0 5 264 2 0.0 4 0 0 0 0 2 265 2 0.0 4 0 0 0 0 2 269 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.14 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 111,300 Reads with adapters: 26,638 (23.9%) Reads written (passing filters): 111,300 (100.0%) Total basepairs processed: 30,245,750 bp Total written (filtered): 29,919,834 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 26638 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 10.3% G: 83.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 105 1739.1 0 105 4 34 434.8 0 34 5 2 108.7 0 2 6 1 27.2 0 1 7 11 6.8 0 11 8 22 1.7 0 22 9 5325 0.4 0 694 4631 10 15391 0.1 1 1323 14068 11 5255 0.0 1 259 4996 12 119 0.0 1 1 118 13 51 0.0 1 0 51 14 10 0.0 1 0 10 18 1 0.0 1 1 19 1 0.0 1 1 38 1 0.0 1 1 41 1 0.0 1 0 1 67 1 0.0 1 0 1 70 1 0.0 1 0 1 86 1 0.0 1 1 147 1 0.0 1 0 1 150 16 0.0 1 11 5 151 1 0.0 1 1 152 2 0.0 1 2 161 1 0.0 1 0 1 163 1 0.0 1 1 165 1 0.0 1 1 169 1 0.0 1 1 177 2 0.0 1 2 189 6 0.0 1 1 5 190 1 0.0 1 0 1 208 207 0.0 1 159 48 209 13 0.0 1 12 1 210 6 0.0 1 6 211 2 0.0 1 2 223 22 0.0 1 20 2 224 3 0.0 1 3 225 1 0.0 1 1 228 4 0.0 1 3 1 232 2 0.0 1 2 235 4 0.0 1 4 252 1 0.0 1 0 1 257 4 0.0 1 4 258 1 0.0 1 1 262 1 0.0 1 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.