This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.66 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 271,311 (10.5%) Reads that were too short: 3,723 (0.1%) Reads written (passing filters): 267,588 (10.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 76,319,943 bp (9.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 271311 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3327 628.5 0 3327 7 4785 628.5 0 4785 8 117908 628.5 0 117908 9 123673 628.5 0 123673 10 137 628.5 0 137 11 28 628.5 0 28 12 42 628.5 0 42 13 19 628.5 0 19 14 18 628.5 0 18 15 15 628.5 0 15 16 33 628.5 0 33 17 16 628.5 0 16 18 25 628.5 0 25 19 23 628.5 0 23 20 18 628.5 0 18 21 15 628.5 0 15 22 29 628.5 0 29 23 21 628.5 0 21 24 8 628.5 0 8 25 17 628.5 0 17 26 16 628.5 0 16 27 16 628.5 0 16 28 16 628.5 0 16 29 18 628.5 0 18 30 26 628.5 0 26 31 10 628.5 0 10 32 16 628.5 0 16 33 13 628.5 0 13 34 25 628.5 0 25 35 24 628.5 0 24 36 20 628.5 0 20 37 25 628.5 0 25 38 18 628.5 0 18 39 15 628.5 0 15 40 21 628.5 0 21 41 18 628.5 0 18 42 14 628.5 0 14 43 17 628.5 0 17 44 14 628.5 0 14 45 24 628.5 0 24 46 20 628.5 0 20 47 34 628.5 0 34 48 24 628.5 0 24 49 34 628.5 0 34 50 23 628.5 0 23 51 22 628.5 0 22 52 24 628.5 0 24 53 37 628.5 0 37 54 24 628.5 0 24 55 30 628.5 0 30 56 29 628.5 0 29 57 17 628.5 0 17 58 18 628.5 0 18 59 36 628.5 0 36 60 29 628.5 0 29 61 22 628.5 0 22 62 30 628.5 0 30 63 23 628.5 0 23 64 19 628.5 0 19 65 13 628.5 0 13 66 53 628.5 0 53 67 46 628.5 0 46 68 80 628.5 0 80 69 11 628.5 0 11 70 33 628.5 0 33 71 16 628.5 0 16 72 24 628.5 0 24 73 23 628.5 0 23 74 18 628.5 0 18 75 25 628.5 0 25 76 31 628.5 0 31 77 32 628.5 0 32 78 24 628.5 0 24 79 37 628.5 0 37 80 29 628.5 0 29 81 21 628.5 0 21 82 34 628.5 0 34 83 36 628.5 0 36 84 25 628.5 0 25 85 38 628.5 0 38 86 30 628.5 0 30 87 19 628.5 0 19 88 38 628.5 0 38 89 35 628.5 0 35 90 28 628.5 0 28 91 36 628.5 0 36 92 36 628.5 0 36 93 39 628.5 0 39 94 29 628.5 0 29 95 24 628.5 0 24 96 31 628.5 0 31 97 32 628.5 0 32 98 36 628.5 0 36 99 25 628.5 0 25 100 28 628.5 0 28 101 40 628.5 0 40 102 36 628.5 0 36 103 50 628.5 0 50 104 111 628.5 0 111 105 33 628.5 0 33 106 45 628.5 0 45 107 75 628.5 0 75 108 43 628.5 0 43 109 43 628.5 0 43 110 63 628.5 0 63 111 246 628.5 0 246 112 567 628.5 0 567 113 177 628.5 0 177 114 75 628.5 0 75 115 197 628.5 0 197 116 182 628.5 0 182 117 1435 628.5 0 1435 118 2616 628.5 0 2616 119 5636 628.5 0 5636 120 35 628.5 0 35 121 21 628.5 0 21 122 32 628.5 0 32 123 21 628.5 0 21 124 22 628.5 0 22 125 22 628.5 0 22 126 27 628.5 0 27 127 41 628.5 0 41 128 35 628.5 0 35 129 22 628.5 0 22 130 32 628.5 0 32 131 33 628.5 0 33 132 40 628.5 0 40 133 30 628.5 0 30 134 16 628.5 0 16 135 27 628.5 0 27 136 28 628.5 0 28 137 27 628.5 0 27 138 21 628.5 0 21 139 23 628.5 0 23 140 18 628.5 0 18 141 27 628.5 0 27 142 21 628.5 0 21 143 19 628.5 0 19 144 31 628.5 0 31 145 28 628.5 0 28 146 42 628.5 0 42 147 35 628.5 0 35 148 46 628.5 0 46 149 35 628.5 0 35 150 27 628.5 0 27 151 257 628.5 0 257 152 200 628.5 0 200 153 353 628.5 0 353 154 254 628.5 0 254 155 30 628.5 0 30 156 36 628.5 0 36 157 23 628.5 0 23 158 10 628.5 0 10 159 24 628.5 0 24 160 22 628.5 0 22 161 33 628.5 0 33 162 69 628.5 0 69 163 69 628.5 0 69 164 97 628.5 0 97 165 28 628.5 0 28 166 26 628.5 0 26 167 8 628.5 0 8 168 21 628.5 0 21 169 24 628.5 0 24 170 10 628.5 0 10 171 31 628.5 0 31 172 20 628.5 0 20 173 29 628.5 0 29 174 13 628.5 0 13 175 12 628.5 0 12 176 28 628.5 0 28 177 12 628.5 0 12 178 30 628.5 0 30 179 153 628.5 0 153 180 79 628.5 0 79 181 105 628.5 0 105 182 80 628.5 0 80 183 299 628.5 0 299 184 25 628.5 0 25 185 74 628.5 0 74 186 12 628.5 0 12 187 30 628.5 0 30 188 7 628.5 0 7 189 17 628.5 0 17 190 24 628.5 0 24 191 30 628.5 0 30 192 27 628.5 0 27 193 27 628.5 0 27 194 16 628.5 0 16 195 16 628.5 0 16 196 20 628.5 0 20 197 19 628.5 0 19 198 11 628.5 0 11 199 17 628.5 0 17 200 23 628.5 0 23 201 21 628.5 0 21 202 19 628.5 0 19 203 21 628.5 0 21 204 16 628.5 0 16 205 13 628.5 0 13 206 18 628.5 0 18 207 13 628.5 0 13 208 15 628.5 0 15 209 12 628.5 0 12 210 34 628.5 0 34 211 24 628.5 0 24 212 23 628.5 0 23 213 28 628.5 0 28 214 39 628.5 0 39 215 18 628.5 0 18 216 34 628.5 0 34 217 27 628.5 0 27 218 111 628.5 0 111 219 140 628.5 0 140 220 245 628.5 0 245 221 56 628.5 0 56 222 16 628.5 0 16 223 26 628.5 0 26 224 25 628.5 0 25 225 32 628.5 0 32 226 21 628.5 0 21 227 23 628.5 0 23 228 29 628.5 0 29 229 29 628.5 0 29 230 42 628.5 0 42 231 61 628.5 0 61 232 65 628.5 0 65 233 80 628.5 0 80 234 35 628.5 0 35 235 60 628.5 0 60 236 60 628.5 0 60 237 74 628.5 0 74 238 26 628.5 0 26 239 24 628.5 0 24 240 25 628.5 0 25 241 15 628.5 0 15 242 19 628.5 0 19 243 23 628.5 0 23 244 22 628.5 0 22 245 34 628.5 0 34 246 36 628.5 0 36 247 41 628.5 0 41 248 40 628.5 0 40 249 32 628.5 0 32 250 32 628.5 0 32 251 28 628.5 0 28 252 35 628.5 0 35 253 40 628.5 0 40 254 51 628.5 0 51 255 27 628.5 0 27 256 20 628.5 0 20 257 19 628.5 0 19 258 20 628.5 0 20 259 30 628.5 0 30 260 18 628.5 0 18 261 11 628.5 0 11 262 6 628.5 0 6 263 21 628.5 0 21 264 25 628.5 0 25 265 23 628.5 0 23 266 21 628.5 0 21 267 32 628.5 0 32 268 27 628.5 0 27 269 26 628.5 0 26 270 24 628.5 0 24 271 47 628.5 0 47 272 36 628.5 0 36 273 24 628.5 0 24 274 28 628.5 0 28 275 26 628.5 0 26 276 24 628.5 0 24 277 21 628.5 0 21 278 70 628.5 0 70 279 54 628.5 0 54 280 69 628.5 0 69 281 22 628.5 0 22 282 29 628.5 0 29 283 30 628.5 0 30 284 37 628.5 0 37 285 47 628.5 0 47 286 29 628.5 0 29 287 39 628.5 0 39 288 34 628.5 0 34 289 31 628.5 0 31 290 28 628.5 0 28 291 46 628.5 0 46 292 31 628.5 0 31 293 41 628.5 0 41 294 33 628.5 0 33 295 37 628.5 0 37 296 35 628.5 0 35 297 59 628.5 0 59 298 48 628.5 0 48 299 96 628.5 0 96 300 61 628.5 0 61 301 54 628.5 0 54 Finished in 11.58 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 267,588 Reads with adapters: 117,661 (44.0%) Reads that were too short: 136 (0.1%) Reads written (passing filters): 117,525 (43.9%) Total basepairs processed: 76,319,943 bp Total written (filtered): 32,420,692 bp (42.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 117661 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 21 0.0 3 0 0 21 16 715 0.0 3 0 696 19 17 112644 0.0 3 110655 1939 48 2 18 3828 0.0 3 3506 313 8 1 19 55 0.0 3 1 11 35 8 20 127 0.0 3 1 0 1 125 21 9 0.0 3 0 0 0 9 22 3 0.0 3 1 0 1 1 23 1 0.0 3 1 24 3 0.0 3 1 1 1 26 1 0.0 3 1 28 2 0.0 3 2 30 1 0.0 3 1 31 1 0.0 3 1 35 1 0.0 3 1 36 1 0.0 3 1 38 1 0.0 3 1 40 10 0.0 3 1 0 0 9 48 1 0.0 3 0 0 0 1 54 51 0.0 3 0 0 0 51 56 10 0.0 3 0 0 0 10 57 6 0.0 3 0 0 1 5 58 9 0.0 3 0 0 0 9 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 1 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 136 1 0.0 3 1 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 9 0.0 3 0 0 0 9 167 3 0.0 3 0 0 0 3 168 4 0.0 3 0 0 0 4 169 8 0.0 3 0 0 1 7 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 1 6 194 2 0.0 3 0 0 1 1 195 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 75 0.0 3 0 0 2 73 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 267 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 0 2 281 8 0.0 3 0 0 0 8 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.11 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 117,525 Reads with adapters: 534 (0.5%) Reads written (passing filters): 117,525 (100.0%) Total basepairs processed: 32,420,692 bp Total written (filtered): 32,418,829 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 534 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.9% C: 6.2% G: 39.9% T: 5.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 293 1836.3 0 293 4 230 459.1 0 230 5 5 114.8 0 5 6 3 28.7 0 3 7 3 7.2 0 3