This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.86 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 519,679 (18.2%) Reads that were too short: 45,437 (1.6%) Reads written (passing filters): 474,242 (16.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 121,482,067 bp (14.1%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 519679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1269 696.4 0 1269 7 32117 696.4 0 32117 8 26240 696.4 0 26240 9 275 696.4 0 275 10 355 696.4 0 355 11 169 696.4 0 169 12 206 696.4 0 206 13 508 696.4 0 508 14 477 696.4 0 477 15 525 696.4 0 525 16 331 696.4 0 331 17 731 696.4 0 731 18 878 696.4 0 878 19 2009 696.4 0 2009 20 30401 696.4 0 30401 21 20022 696.4 0 20022 22 28832 696.4 0 28832 23 330 696.4 0 330 24 214 696.4 0 214 25 324 696.4 0 324 26 149 696.4 0 149 27 120 696.4 0 120 28 80 696.4 0 80 29 160 696.4 0 160 30 107 696.4 0 107 31 208 696.4 0 208 32 376 696.4 0 376 33 452 696.4 0 452 34 519 696.4 0 519 35 1062 696.4 0 1062 36 2307 696.4 0 2307 37 609 696.4 0 609 38 854 696.4 0 854 39 1889 696.4 0 1889 40 685 696.4 0 685 41 680 696.4 0 680 42 1405 696.4 0 1405 43 1352 696.4 0 1352 44 1706 696.4 0 1706 45 1160 696.4 0 1160 46 2220 696.4 0 2220 47 2873 696.4 0 2873 48 6190 696.4 0 6190 49 91327 696.4 0 91327 50 67381 696.4 0 67381 51 115017 696.4 0 115017 52 505 696.4 0 505 53 51 696.4 0 51 54 65 696.4 0 65 55 49 696.4 0 49 56 69 696.4 0 69 57 60 696.4 0 60 58 83 696.4 0 83 59 60 696.4 0 60 60 107 696.4 0 107 61 71 696.4 0 71 62 191 696.4 0 191 63 1051 696.4 0 1051 64 1020 696.4 0 1020 65 1277 696.4 0 1277 66 28 696.4 0 28 67 43 696.4 0 43 68 35 696.4 0 35 69 36 696.4 0 36 70 34 696.4 0 34 71 44 696.4 0 44 72 43 696.4 0 43 73 32 696.4 0 32 74 41 696.4 0 41 75 48 696.4 0 48 76 34 696.4 0 34 77 27 696.4 0 27 78 33 696.4 0 33 79 33 696.4 0 33 80 46 696.4 0 46 81 30 696.4 0 30 82 29 696.4 0 29 83 28 696.4 0 28 84 61 696.4 0 61 85 32 696.4 0 32 86 51 696.4 0 51 87 57 696.4 0 57 88 141 696.4 0 141 89 50 696.4 0 50 90 72 696.4 0 72 91 116 696.4 0 116 92 49 696.4 0 49 93 42 696.4 0 42 94 45 696.4 0 45 95 31 696.4 0 31 96 36 696.4 0 36 97 29 696.4 0 29 98 44 696.4 0 44 99 26 696.4 0 26 100 23 696.4 0 23 101 33 696.4 0 33 102 31 696.4 0 31 103 20 696.4 0 20 104 36 696.4 0 36 105 31 696.4 0 31 106 42 696.4 0 42 107 35 696.4 0 35 108 20 696.4 0 20 109 23 696.4 0 23 110 33 696.4 0 33 111 41 696.4 0 41 112 29 696.4 0 29 113 45 696.4 0 45 114 26 696.4 0 26 115 34 696.4 0 34 116 45 696.4 0 45 117 309 696.4 0 309 118 384 696.4 0 384 119 474 696.4 0 474 120 72 696.4 0 72 121 72 696.4 0 72 122 23 696.4 0 23 123 36 696.4 0 36 124 99 696.4 0 99 125 79 696.4 0 79 126 137 696.4 0 137 127 44 696.4 0 44 128 64 696.4 0 64 129 34 696.4 0 34 130 53 696.4 0 53 131 39 696.4 0 39 132 41 696.4 0 41 133 33 696.4 0 33 134 36 696.4 0 36 135 47 696.4 0 47 136 28 696.4 0 28 137 30 696.4 0 30 138 42 696.4 0 42 139 43 696.4 0 43 140 49 696.4 0 49 141 40 696.4 0 40 142 46 696.4 0 46 143 71 696.4 0 71 144 101 696.4 0 101 145 109 696.4 0 109 146 72 696.4 0 72 147 50 696.4 0 50 148 41 696.4 0 41 149 42 696.4 0 42 150 42 696.4 0 42 151 53 696.4 0 53 152 57 696.4 0 57 153 66 696.4 0 66 154 53 696.4 0 53 155 63 696.4 0 63 156 65 696.4 0 65 157 134 696.4 0 134 158 125 696.4 0 125 159 223 696.4 0 223 160 53 696.4 0 53 161 43 696.4 0 43 162 38 696.4 0 38 163 43 696.4 0 43 164 33 696.4 0 33 165 36 696.4 0 36 166 32 696.4 0 32 167 52 696.4 0 52 168 37 696.4 0 37 169 48 696.4 0 48 170 68 696.4 0 68 171 58 696.4 0 58 172 55 696.4 0 55 173 60 696.4 0 60 174 43 696.4 0 43 175 45 696.4 0 45 176 83 696.4 0 83 177 121 696.4 0 121 178 131 696.4 0 131 179 172 696.4 0 172 180 188 696.4 0 188 181 97 696.4 0 97 182 166 696.4 0 166 183 271 696.4 0 271 184 469 696.4 0 469 185 84 696.4 0 84 186 104 696.4 0 104 187 116 696.4 0 116 188 175 696.4 0 175 189 175 696.4 0 175 190 160 696.4 0 160 191 168 696.4 0 168 192 282 696.4 0 282 193 2238 696.4 0 2238 194 2222 696.4 0 2222 195 6607 696.4 0 6607 196 1381 696.4 0 1381 197 170 696.4 0 170 198 62 696.4 0 62 199 121 696.4 0 121 200 90 696.4 0 90 201 91 696.4 0 91 202 56 696.4 0 56 203 64 696.4 0 64 204 41 696.4 0 41 205 65 696.4 0 65 206 110 696.4 0 110 207 57 696.4 0 57 208 91 696.4 0 91 209 94 696.4 0 94 210 68 696.4 0 68 211 52 696.4 0 52 212 84 696.4 0 84 213 328 696.4 0 328 214 734 696.4 0 734 215 704 696.4 0 704 216 557 696.4 0 557 217 212 696.4 0 212 218 298 696.4 0 298 219 1221 696.4 0 1221 220 768 696.4 0 768 221 904 696.4 0 904 222 357 696.4 0 357 223 178 696.4 0 178 224 70 696.4 0 70 225 96 696.4 0 96 226 89 696.4 0 89 227 110 696.4 0 110 228 96 696.4 0 96 229 156 696.4 0 156 230 78 696.4 0 78 231 87 696.4 0 87 232 66 696.4 0 66 233 104 696.4 0 104 234 89 696.4 0 89 235 85 696.4 0 85 236 184 696.4 0 184 237 129 696.4 0 129 238 238 696.4 0 238 239 1048 696.4 0 1048 240 1127 696.4 0 1127 241 988 696.4 0 988 242 349 696.4 0 349 243 58 696.4 0 58 244 97 696.4 0 97 245 91 696.4 0 91 246 163 696.4 0 163 247 92 696.4 0 92 248 110 696.4 0 110 249 200 696.4 0 200 250 572 696.4 0 572 251 1474 696.4 0 1474 252 1252 696.4 0 1252 253 1277 696.4 0 1277 254 71 696.4 0 71 255 106 696.4 0 106 256 127 696.4 0 127 257 179 696.4 0 179 258 151 696.4 0 151 259 356 696.4 0 356 260 214 696.4 0 214 261 374 696.4 0 374 262 146 696.4 0 146 263 842 696.4 0 842 264 464 696.4 0 464 265 1139 696.4 0 1139 266 529 696.4 0 529 267 341 696.4 0 341 268 351 696.4 0 351 269 217 696.4 0 217 270 319 696.4 0 319 271 155 696.4 0 155 272 159 696.4 0 159 273 65 696.4 0 65 274 98 696.4 0 98 275 193 696.4 0 193 276 132 696.4 0 132 277 138 696.4 0 138 278 145 696.4 0 145 279 101 696.4 0 101 280 93 696.4 0 93 281 163 696.4 0 163 282 192 696.4 0 192 283 345 696.4 0 345 284 243 696.4 0 243 285 291 696.4 0 291 286 299 696.4 0 299 287 680 696.4 0 680 288 5732 696.4 0 5732 289 4319 696.4 0 4319 290 4915 696.4 0 4915 291 464 696.4 0 464 292 167 696.4 0 167 293 317 696.4 0 317 294 432 696.4 0 432 295 296 696.4 0 296 296 165 696.4 0 165 297 282 696.4 0 282 298 244 696.4 0 244 299 380 696.4 0 380 300 389 696.4 0 389 301 299 696.4 0 299 Finished in 22.18 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 474,242 Reads with adapters: 34,439 (7.3%) Reads that were too short: 340 (0.1%) Reads written (passing filters): 34,099 (7.2%) Total basepairs processed: 121,482,067 bp Total written (filtered): 8,657,845 bp (7.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 34439 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 14 0.0 3 0 0 10 2 2 19 358 0.0 3 0 307 24 17 10 20 27434 0.0 4 25940 1246 204 33 11 21 59 0.0 4 12 45 1 1 22 7 0.0 4 2 1 1 1 2 23 84 0.0 4 0 0 0 56 28 24 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 49 4 0.0 4 0 0 0 1 3 50 4 0.0 4 0 0 0 0 4 51 1 0.0 4 0 0 0 0 1 63 2 0.0 4 1 0 0 0 1 64 1 0.0 4 0 0 1 65 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 1 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 11 0.0 4 0 0 0 0 11 84 27 0.0 4 0 0 0 0 27 85 5021 0.0 4 0 0 0 1 5020 87 1 0.0 4 0 0 0 0 1 88 8 0.0 4 0 0 0 0 8 92 1 0.0 4 0 0 0 0 1 97 9 0.0 4 0 0 0 0 9 98 5 0.0 4 0 0 0 0 5 100 1 0.0 4 0 0 1 102 1 0.0 4 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 8 0.0 4 0 0 0 0 8 112 6 0.0 4 0 0 0 0 6 113 5 0.0 4 0 0 0 0 5 114 832 0.0 4 0 0 0 0 832 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 1 117 3 0.0 4 0 0 0 0 3 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 155 0.0 4 0 0 0 0 155 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 11 0.0 4 0 0 0 1 10 142 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 172 12 0.0 4 0 0 0 0 12 173 11 0.0 4 0 0 0 1 10 174 2 0.0 4 0 0 0 0 2 175 3 0.0 4 0 0 0 1 2 176 1 0.0 4 0 0 0 0 1 177 3 0.0 4 0 0 0 0 3 178 1 0.0 4 0 0 0 0 1 182 3 0.0 4 0 0 0 1 2 183 3 0.0 4 0 0 0 0 3 186 22 0.0 4 0 0 0 0 22 187 4 0.0 4 0 0 0 1 3 188 2 0.0 4 0 0 0 0 2 190 2 0.0 4 0 0 0 0 2 192 6 0.0 4 0 0 0 0 6 193 3 0.0 4 0 0 0 0 3 195 2 0.0 4 0 0 0 0 2 196 3 0.0 4 0 0 0 0 3 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 1 206 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 210 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 8 0.0 4 0 0 0 0 8 216 6 0.0 4 0 0 0 0 6 218 7 0.0 4 0 0 0 0 7 219 2 0.0 4 0 0 0 0 2 220 3 0.0 4 0 0 0 0 3 221 17 0.0 4 0 0 0 1 16 222 27 0.0 4 0 0 0 1 26 223 28 0.0 4 0 0 0 2 26 224 7 0.0 4 0 0 0 0 7 225 9 0.0 4 0 0 0 0 9 226 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 1 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 1 0 0 0 1 240 7 0.0 4 0 0 0 1 6 241 4 0.0 4 0 0 0 0 4 242 1 0.0 4 0 0 0 0 1 243 4 0.0 4 0 0 0 0 4 244 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 246 8 0.0 4 0 0 0 0 8 247 3 0.0 4 0 0 0 0 3 249 3 0.0 4 0 0 0 0 3 250 7 0.0 4 0 0 0 0 7 251 18 0.0 4 0 0 0 2 16 252 12 0.0 4 0 0 0 0 12 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 3 0.0 4 0 0 0 0 3 264 2 0.0 4 0 0 0 0 2 265 3 0.0 4 0 0 0 0 3 268 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 279 2 0.0 4 0 0 0 0 2 280 2 0.0 4 0 0 0 0 2 281 4 0.0 4 0 0 0 0 4 293 2 0.0 4 0 0 0 0 2 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.18 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 34,099 Reads with adapters: 5,091 (14.9%) Reads written (passing filters): 34,099 (100.0%) Total basepairs processed: 8,657,845 bp Total written (filtered): 8,601,989 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5091 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 9.1% G: 84.8% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 58 532.8 0 58 4 38 133.2 0 38 5 3 33.3 0 3 9 24 0.1 0 2 22 10 1544 0.0 1 159 1385 11 3288 0.0 1 178 3110 12 90 0.0 1 4 86 13 11 0.0 1 0 11 14 8 0.0 1 0 8 15 2 0.0 1 0 2 19 12 0.0 1 7 5 20 2 0.0 1 2 73 1 0.0 1 1 151 1 0.0 1 1 153 2 0.0 1 1 1 209 5 0.0 1 5 224 1 0.0 1 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.