This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.16 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 460,233 (16.1%) Reads that were too short: 17,232 (0.6%) Reads written (passing filters): 443,001 (15.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 113,933,168 bp (13.3%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 460233 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1186 696.4 0 1186 7 30129 696.4 0 30129 8 30668 696.4 0 30668 9 242 696.4 0 242 10 273 696.4 0 273 11 152 696.4 0 152 12 166 696.4 0 166 13 434 696.4 0 434 14 410 696.4 0 410 15 445 696.4 0 445 16 235 696.4 0 235 17 592 696.4 0 592 18 638 696.4 0 638 19 1397 696.4 0 1397 20 27611 696.4 0 27611 21 18381 696.4 0 18381 22 26578 696.4 0 26578 23 291 696.4 0 291 24 186 696.4 0 186 25 301 696.4 0 301 26 150 696.4 0 150 27 137 696.4 0 137 28 86 696.4 0 86 29 155 696.4 0 155 30 110 696.4 0 110 31 154 696.4 0 154 32 305 696.4 0 305 33 456 696.4 0 456 34 529 696.4 0 529 35 1065 696.4 0 1065 36 2323 696.4 0 2323 37 578 696.4 0 578 38 769 696.4 0 769 39 1893 696.4 0 1893 40 580 696.4 0 580 41 624 696.4 0 624 42 1318 696.4 0 1318 43 1295 696.4 0 1295 44 1540 696.4 0 1540 45 960 696.4 0 960 46 1815 696.4 0 1815 47 2198 696.4 0 2198 48 4178 696.4 0 4178 49 81848 696.4 0 81848 50 62872 696.4 0 62872 51 113908 696.4 0 113908 52 421 696.4 0 421 53 48 696.4 0 48 54 42 696.4 0 42 55 30 696.4 0 30 56 41 696.4 0 41 57 38 696.4 0 38 58 56 696.4 0 56 59 39 696.4 0 39 60 68 696.4 0 68 61 39 696.4 0 39 62 79 696.4 0 79 63 281 696.4 0 281 64 353 696.4 0 353 65 574 696.4 0 574 66 33 696.4 0 33 67 37 696.4 0 37 68 29 696.4 0 29 69 33 696.4 0 33 70 28 696.4 0 28 71 25 696.4 0 25 72 30 696.4 0 30 73 36 696.4 0 36 74 36 696.4 0 36 75 48 696.4 0 48 76 37 696.4 0 37 77 40 696.4 0 40 78 33 696.4 0 33 79 40 696.4 0 40 80 35 696.4 0 35 81 35 696.4 0 35 82 25 696.4 0 25 83 45 696.4 0 45 84 46 696.4 0 46 85 41 696.4 0 41 86 40 696.4 0 40 87 41 696.4 0 41 88 41 696.4 0 41 89 37 696.4 0 37 90 38 696.4 0 38 91 39 696.4 0 39 92 42 696.4 0 42 93 50 696.4 0 50 94 25 696.4 0 25 95 28 696.4 0 28 96 38 696.4 0 38 97 34 696.4 0 34 98 52 696.4 0 52 99 37 696.4 0 37 100 21 696.4 0 21 101 40 696.4 0 40 102 28 696.4 0 28 103 25 696.4 0 25 104 28 696.4 0 28 105 33 696.4 0 33 106 54 696.4 0 54 107 42 696.4 0 42 108 27 696.4 0 27 109 37 696.4 0 37 110 46 696.4 0 46 111 34 696.4 0 34 112 37 696.4 0 37 113 40 696.4 0 40 114 36 696.4 0 36 115 39 696.4 0 39 116 50 696.4 0 50 117 153 696.4 0 153 118 185 696.4 0 185 119 192 696.4 0 192 120 36 696.4 0 36 121 42 696.4 0 42 122 34 696.4 0 34 123 42 696.4 0 42 124 66 696.4 0 66 125 51 696.4 0 51 126 74 696.4 0 74 127 48 696.4 0 48 128 36 696.4 0 36 129 28 696.4 0 28 130 65 696.4 0 65 131 45 696.4 0 45 132 27 696.4 0 27 133 33 696.4 0 33 134 45 696.4 0 45 135 37 696.4 0 37 136 38 696.4 0 38 137 28 696.4 0 28 138 49 696.4 0 49 139 30 696.4 0 30 140 38 696.4 0 38 141 41 696.4 0 41 142 47 696.4 0 47 143 57 696.4 0 57 144 63 696.4 0 63 145 39 696.4 0 39 146 73 696.4 0 73 147 58 696.4 0 58 148 31 696.4 0 31 149 46 696.4 0 46 150 42 696.4 0 42 151 41 696.4 0 41 152 58 696.4 0 58 153 50 696.4 0 50 154 51 696.4 0 51 155 44 696.4 0 44 156 45 696.4 0 45 157 75 696.4 0 75 158 67 696.4 0 67 159 97 696.4 0 97 160 35 696.4 0 35 161 42 696.4 0 42 162 37 696.4 0 37 163 37 696.4 0 37 164 37 696.4 0 37 165 38 696.4 0 38 166 45 696.4 0 45 167 42 696.4 0 42 168 33 696.4 0 33 169 52 696.4 0 52 170 44 696.4 0 44 171 42 696.4 0 42 172 32 696.4 0 32 173 57 696.4 0 57 174 42 696.4 0 42 175 56 696.4 0 56 176 64 696.4 0 64 177 64 696.4 0 64 178 78 696.4 0 78 179 104 696.4 0 104 180 130 696.4 0 130 181 78 696.4 0 78 182 147 696.4 0 147 183 288 696.4 0 288 184 568 696.4 0 568 185 87 696.4 0 87 186 97 696.4 0 97 187 93 696.4 0 93 188 141 696.4 0 141 189 109 696.4 0 109 190 111 696.4 0 111 191 143 696.4 0 143 192 150 696.4 0 150 193 2031 696.4 0 2031 194 1837 696.4 0 1837 195 5897 696.4 0 5897 196 1101 696.4 0 1101 197 138 696.4 0 138 198 52 696.4 0 52 199 119 696.4 0 119 200 58 696.4 0 58 201 57 696.4 0 57 202 48 696.4 0 48 203 59 696.4 0 59 204 28 696.4 0 28 205 69 696.4 0 69 206 66 696.4 0 66 207 37 696.4 0 37 208 54 696.4 0 54 209 51 696.4 0 51 210 53 696.4 0 53 211 53 696.4 0 53 212 62 696.4 0 62 213 133 696.4 0 133 214 268 696.4 0 268 215 273 696.4 0 273 216 206 696.4 0 206 217 80 696.4 0 80 218 105 696.4 0 105 219 365 696.4 0 365 220 222 696.4 0 222 221 274 696.4 0 274 222 118 696.4 0 118 223 63 696.4 0 63 224 53 696.4 0 53 225 58 696.4 0 58 226 69 696.4 0 69 227 58 696.4 0 58 228 49 696.4 0 49 229 112 696.4 0 112 230 46 696.4 0 46 231 62 696.4 0 62 232 51 696.4 0 51 233 49 696.4 0 49 234 46 696.4 0 46 235 44 696.4 0 44 236 101 696.4 0 101 237 67 696.4 0 67 238 74 696.4 0 74 239 315 696.4 0 315 240 405 696.4 0 405 241 331 696.4 0 331 242 146 696.4 0 146 243 40 696.4 0 40 244 43 696.4 0 43 245 40 696.4 0 40 246 64 696.4 0 64 247 44 696.4 0 44 248 78 696.4 0 78 249 122 696.4 0 122 250 259 696.4 0 259 251 743 696.4 0 743 252 613 696.4 0 613 253 627 696.4 0 627 254 40 696.4 0 40 255 54 696.4 0 54 256 51 696.4 0 51 257 76 696.4 0 76 258 75 696.4 0 75 259 115 696.4 0 115 260 80 696.4 0 80 261 139 696.4 0 139 262 69 696.4 0 69 263 268 696.4 0 268 264 149 696.4 0 149 265 379 696.4 0 379 266 206 696.4 0 206 267 132 696.4 0 132 268 123 696.4 0 123 269 73 696.4 0 73 270 112 696.4 0 112 271 82 696.4 0 82 272 94 696.4 0 94 273 66 696.4 0 66 274 70 696.4 0 70 275 74 696.4 0 74 276 70 696.4 0 70 277 60 696.4 0 60 278 83 696.4 0 83 279 55 696.4 0 55 280 58 696.4 0 58 281 58 696.4 0 58 282 94 696.4 0 94 283 126 696.4 0 126 284 87 696.4 0 87 285 108 696.4 0 108 286 97 696.4 0 97 287 248 696.4 0 248 288 1711 696.4 0 1711 289 1467 696.4 0 1467 290 1679 696.4 0 1679 291 196 696.4 0 196 292 75 696.4 0 75 293 128 696.4 0 128 294 98 696.4 0 98 295 78 696.4 0 78 296 64 696.4 0 64 297 74 696.4 0 74 298 69 696.4 0 69 299 113 696.4 0 113 300 186 696.4 0 186 301 127 696.4 0 127 Finished in 18.12 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 443,001 Reads with adapters: 33,309 (7.5%) Reads that were too short: 1,167 (0.3%) Reads written (passing filters): 32,142 (7.3%) Total basepairs processed: 113,933,168 bp Total written (filtered): 8,410,575 bp (7.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33309 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 190 0.0 3 0 181 9 17 28422 0.0 3 27877 528 16 1 18 65 0.0 3 4 60 0 1 19 13 0.0 3 0 0 11 2 20 44 0.0 3 0 0 0 44 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 25 2 0.0 3 2 27 2 0.0 3 2 28 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 60 1 0.0 3 1 64 1 0.0 3 0 0 0 1 69 1 0.0 3 1 70 1 0.0 3 1 71 2 0.0 3 1 0 0 1 72 5 0.0 3 0 0 0 5 73 364 0.0 3 0 0 2 362 74 10 0.0 3 0 0 0 10 75 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 64 0.0 3 0 0 0 64 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 93 7 0.0 3 0 0 4 3 94 1 0.0 3 0 0 0 1 97 26 0.0 3 0 0 0 26 98 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 102 18 0.0 3 0 0 0 18 103 3 0.0 3 0 0 0 3 105 1 0.0 3 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 4 0.0 3 0 0 0 4 118 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 121 4 0.0 3 0 0 0 4 122 733 0.0 3 0 0 2 731 123 634 0.0 3 0 0 2 632 124 137 0.0 3 0 0 0 137 125 248 0.0 3 0 0 3 245 126 86 0.0 3 0 0 3 83 127 26 0.0 3 0 0 1 25 128 85 0.0 3 0 0 0 85 129 3 0.0 3 0 0 0 3 130 25 0.0 3 0 0 0 25 131 9 0.0 3 0 0 0 9 132 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 6 0.0 3 0 0 0 6 135 2 0.0 3 0 0 0 2 136 6 0.0 3 0 0 0 6 137 26 0.0 3 0 0 0 26 138 14 0.0 3 0 0 0 14 139 24 0.0 3 0 0 0 24 140 12 0.0 3 0 0 0 12 141 3 0.0 3 0 0 0 3 142 159 0.0 3 0 0 0 159 143 7 0.0 3 0 0 0 7 144 2 0.0 3 0 0 0 2 145 19 0.0 3 0 0 1 18 146 10 0.0 3 0 0 0 10 147 1 0.0 3 0 0 0 1 148 16 0.0 3 0 0 0 16 149 12 0.0 3 0 0 0 12 150 3 0.0 3 0 0 0 3 151 147 0.0 3 0 0 1 146 152 43 0.0 3 0 0 0 43 153 15 0.0 3 0 0 0 15 154 32 0.0 3 0 0 3 29 155 7 0.0 3 0 0 0 7 156 3 0.0 3 0 0 0 3 157 6 0.0 3 0 0 0 6 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 164 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 167 3 0.0 3 0 0 0 3 168 2 0.0 3 0 0 0 2 169 4 0.0 3 0 0 0 4 170 10 0.0 3 0 0 0 10 171 156 0.0 3 0 0 0 156 172 96 0.0 3 0 0 0 96 173 3 0.0 3 0 0 0 3 174 20 0.0 3 0 0 0 20 175 7 0.0 3 0 0 0 7 176 1 0.0 3 0 0 0 1 177 6 0.0 3 0 0 0 6 179 2 0.0 3 0 0 0 2 180 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 4 0.0 3 0 0 0 4 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 3 0.0 3 0 0 0 3 197 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 199 2 0.0 3 0 0 0 2 202 4 0.0 3 0 0 0 4 203 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 217 11 0.0 3 0 0 0 11 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 231 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 475 0.0 3 0 0 2 473 235 84 0.0 3 0 0 0 84 236 53 0.0 3 0 0 0 53 237 88 0.0 3 0 0 3 85 238 145 0.0 3 0 0 3 142 239 22 0.0 3 0 0 0 22 240 33 0.0 3 0 0 1 32 241 3 0.0 3 0 0 0 3 242 8 0.0 3 0 0 0 8 243 8 0.0 3 0 0 0 8 244 12 0.0 3 0 0 1 11 245 5 0.0 3 0 0 0 5 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 3 0.0 3 0 0 0 3 250 9 0.0 3 0 0 0 9 251 5 0.0 3 0 0 0 5 252 5 0.0 3 0 0 1 4 253 5 0.0 3 0 0 1 4 257 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 263 27 0.0 3 0 0 0 27 264 8 0.0 3 0 0 0 8 265 3 0.0 3 0 0 0 3 266 12 0.0 3 0 0 1 11 267 15 0.0 3 0 0 0 15 268 3 0.0 3 0 0 0 3 269 1 0.0 3 0 0 0 1 272 7 0.0 3 0 0 0 7 273 3 0.0 3 0 0 0 3 274 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.11 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 32,142 Reads with adapters: 375 (1.2%) Reads written (passing filters): 32,142 (100.0%) Total basepairs processed: 8,410,575 bp Total written (filtered): 8,409,429 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 375 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.0% C: 13.3% G: 7.7% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 354 502.2 0 354 4 21 125.6 0 21