This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.68 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 469,919 (16.5%) Reads that were too short: 16,102 (0.6%) Reads written (passing filters): 453,817 (15.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 108,997,153 bp (12.7%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 469919 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9176 696.4 0 9176 7 74438 696.4 0 74438 8 2498 696.4 0 2498 9 79712 696.4 0 79712 10 173 696.4 0 173 11 59 696.4 0 59 12 82 696.4 0 82 13 39 696.4 0 39 14 43 696.4 0 43 15 62 696.4 0 62 16 64 696.4 0 64 17 69 696.4 0 69 18 71 696.4 0 71 19 70 696.4 0 70 20 69 696.4 0 69 21 63 696.4 0 63 22 84 696.4 0 84 23 43 696.4 0 43 24 61 696.4 0 61 25 46 696.4 0 46 26 51 696.4 0 51 27 53 696.4 0 53 28 37 696.4 0 37 29 48 696.4 0 48 30 60 696.4 0 60 31 43 696.4 0 43 32 54 696.4 0 54 33 41 696.4 0 41 34 53 696.4 0 53 35 79 696.4 0 79 36 59 696.4 0 59 37 69 696.4 0 69 38 62 696.4 0 62 39 42 696.4 0 42 40 82 696.4 0 82 41 51 696.4 0 51 42 73 696.4 0 73 43 76 696.4 0 76 44 63 696.4 0 63 45 70 696.4 0 70 46 85 696.4 0 85 47 66 696.4 0 66 48 75 696.4 0 75 49 66 696.4 0 66 50 45 696.4 0 45 51 106 696.4 0 106 52 75 696.4 0 75 53 88 696.4 0 88 54 94 696.4 0 94 55 103 696.4 0 103 56 149 696.4 0 149 57 127 696.4 0 127 58 93 696.4 0 93 59 66 696.4 0 66 60 118 696.4 0 118 61 104 696.4 0 104 62 212 696.4 0 212 63 101 696.4 0 101 64 105 696.4 0 105 65 54 696.4 0 54 66 164 696.4 0 164 67 120 696.4 0 120 68 185 696.4 0 185 69 235 696.4 0 235 70 347 696.4 0 347 71 382 696.4 0 382 72 885 696.4 0 885 73 1796 696.4 0 1796 74 564 696.4 0 564 75 696 696.4 0 696 76 1339 696.4 0 1339 77 492 696.4 0 492 78 576 696.4 0 576 79 1207 696.4 0 1207 80 1263 696.4 0 1263 81 1922 696.4 0 1922 82 1257 696.4 0 1257 83 2291 696.4 0 2291 84 2822 696.4 0 2822 85 6537 696.4 0 6537 86 93696 696.4 0 93696 87 65575 696.4 0 65575 88 74479 696.4 0 74479 89 340 696.4 0 340 90 43 696.4 0 43 91 38 696.4 0 38 92 50 696.4 0 50 93 49 696.4 0 49 94 47 696.4 0 47 95 48 696.4 0 48 96 59 696.4 0 59 97 55 696.4 0 55 98 42 696.4 0 42 99 63 696.4 0 63 100 58 696.4 0 58 101 62 696.4 0 62 102 79 696.4 0 79 103 315 696.4 0 315 104 122 696.4 0 122 105 91 696.4 0 91 106 36 696.4 0 36 107 39 696.4 0 39 108 54 696.4 0 54 109 41 696.4 0 41 110 48 696.4 0 48 111 46 696.4 0 46 112 51 696.4 0 51 113 54 696.4 0 54 114 52 696.4 0 52 115 46 696.4 0 46 116 62 696.4 0 62 117 57 696.4 0 57 118 53 696.4 0 53 119 58 696.4 0 58 120 70 696.4 0 70 121 71 696.4 0 71 122 80 696.4 0 80 123 296 696.4 0 296 124 402 696.4 0 402 125 295 696.4 0 295 126 359 696.4 0 359 127 126 696.4 0 126 128 69 696.4 0 69 129 209 696.4 0 209 130 117 696.4 0 117 131 119 696.4 0 119 132 65 696.4 0 65 133 91 696.4 0 91 134 181 696.4 0 181 135 106 696.4 0 106 136 150 696.4 0 150 137 131 696.4 0 131 138 96 696.4 0 96 139 123 696.4 0 123 140 137 696.4 0 137 141 345 696.4 0 345 142 206 696.4 0 206 143 336 696.4 0 336 144 2983 696.4 0 2983 145 2669 696.4 0 2669 146 2563 696.4 0 2563 147 100 696.4 0 100 148 64 696.4 0 64 149 97 696.4 0 97 150 96 696.4 0 96 151 85 696.4 0 85 152 67 696.4 0 67 153 61 696.4 0 61 154 95 696.4 0 95 155 79 696.4 0 79 156 110 696.4 0 110 157 71 696.4 0 71 158 53 696.4 0 53 159 63 696.4 0 63 160 47 696.4 0 47 161 78 696.4 0 78 162 47 696.4 0 47 163 64 696.4 0 64 164 101 696.4 0 101 165 142 696.4 0 142 166 997 696.4 0 997 167 767 696.4 0 767 168 874 696.4 0 874 169 57 696.4 0 57 170 71 696.4 0 71 171 89 696.4 0 89 172 83 696.4 0 83 173 80 696.4 0 80 174 63 696.4 0 63 175 92 696.4 0 92 176 51 696.4 0 51 177 48 696.4 0 48 178 70 696.4 0 70 179 56 696.4 0 56 180 88 696.4 0 88 181 85 696.4 0 85 182 109 696.4 0 109 183 98 696.4 0 98 184 144 696.4 0 144 185 2552 696.4 0 2552 186 655 696.4 0 655 187 433 696.4 0 433 188 82 696.4 0 82 189 120 696.4 0 120 190 73 696.4 0 73 191 72 696.4 0 72 192 80 696.4 0 80 193 154 696.4 0 154 194 110 696.4 0 110 195 102 696.4 0 102 196 81 696.4 0 81 197 56 696.4 0 56 198 56 696.4 0 56 199 70 696.4 0 70 200 83 696.4 0 83 201 93 696.4 0 93 202 67 696.4 0 67 203 107 696.4 0 107 204 104 696.4 0 104 205 155 696.4 0 155 206 2052 696.4 0 2052 207 1034 696.4 0 1034 208 1333 696.4 0 1333 209 93 696.4 0 93 210 130 696.4 0 130 211 1069 696.4 0 1069 212 596 696.4 0 596 213 682 696.4 0 682 214 59 696.4 0 59 215 47 696.4 0 47 216 43 696.4 0 43 217 55 696.4 0 55 218 58 696.4 0 58 219 47 696.4 0 47 220 65 696.4 0 65 221 56 696.4 0 56 222 57 696.4 0 57 223 59 696.4 0 59 224 56 696.4 0 56 225 88 696.4 0 88 226 124 696.4 0 124 227 179 696.4 0 179 228 190 696.4 0 190 229 100 696.4 0 100 230 52 696.4 0 52 231 62 696.4 0 62 232 53 696.4 0 53 233 50 696.4 0 50 234 53 696.4 0 53 235 54 696.4 0 54 236 49 696.4 0 49 237 55 696.4 0 55 238 51 696.4 0 51 239 67 696.4 0 67 240 62 696.4 0 62 241 117 696.4 0 117 242 80 696.4 0 80 243 99 696.4 0 99 244 56 696.4 0 56 245 155 696.4 0 155 246 126 696.4 0 126 247 84 696.4 0 84 248 74 696.4 0 74 249 55 696.4 0 55 250 63 696.4 0 63 251 42 696.4 0 42 252 64 696.4 0 64 253 58 696.4 0 58 254 66 696.4 0 66 255 206 696.4 0 206 256 117 696.4 0 117 257 146 696.4 0 146 258 73 696.4 0 73 259 495 696.4 0 495 260 367 696.4 0 367 261 696 696.4 0 696 262 180 696.4 0 180 263 84 696.4 0 84 264 60 696.4 0 60 265 45 696.4 0 45 266 67 696.4 0 67 267 200 696.4 0 200 268 227 696.4 0 227 269 137 696.4 0 137 270 59 696.4 0 59 271 36 696.4 0 36 272 43 696.4 0 43 273 46 696.4 0 46 274 56 696.4 0 56 275 167 696.4 0 167 276 98 696.4 0 98 277 108 696.4 0 108 278 49 696.4 0 49 279 71 696.4 0 71 280 103 696.4 0 103 281 202 696.4 0 202 282 142 696.4 0 142 283 90 696.4 0 90 284 43 696.4 0 43 285 38 696.4 0 38 286 42 696.4 0 42 287 51 696.4 0 51 288 62 696.4 0 62 289 62 696.4 0 62 290 50 696.4 0 50 291 66 696.4 0 66 292 36 696.4 0 36 293 49 696.4 0 49 294 127 696.4 0 127 295 127 696.4 0 127 296 118 696.4 0 118 297 80 696.4 0 80 298 55 696.4 0 55 299 59 696.4 0 59 300 65 696.4 0 65 301 150 696.4 0 150 Finished in 20.00 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 453,817 Reads with adapters: 80,353 (17.7%) Reads that were too short: 179 (0.0%) Reads written (passing filters): 80,174 (17.7%) Total basepairs processed: 108,997,153 bp Total written (filtered): 21,924,175 bp (20.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 80353 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 54 0.0 3 0 0 46 5 3 19 1604 0.0 3 0 1489 73 32 10 20 76184 0.0 4 72904 2711 421 119 29 21 175 0.0 4 30 127 8 6 4 22 1615 0.0 4 1490 85 26 6 8 23 110 0.0 4 3 2 0 74 31 24 2 0.0 4 0 0 0 1 1 25 9 0.0 4 0 0 0 8 1 26 1 0.0 4 0 1 28 1 0.0 4 1 30 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 15 0.0 4 0 0 0 0 15 50 2 0.0 4 0 0 0 0 2 51 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 4 0.0 4 0 0 0 0 4 74 67 0.0 4 0 0 0 2 65 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 274 0.0 4 0 0 0 1 273 128 1 0.0 4 0 0 0 0 1 129 32 0.0 4 0 0 0 0 32 130 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 1 140 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 3 0.0 4 0 0 0 0 3 148 1 0.0 4 0 0 0 0 1 149 47 0.0 4 0 0 0 2 45 150 10 0.0 4 0 0 0 0 10 151 3 0.0 4 0 0 0 0 3 153 12 0.0 4 0 0 0 0 12 154 5 0.0 4 0 0 0 0 5 155 11 0.0 4 0 0 0 1 10 156 9 0.0 4 0 0 0 0 9 175 2 0.0 4 0 0 0 0 2 177 4 0.0 4 0 0 0 0 4 178 5 0.0 4 0 0 0 0 5 179 5 0.0 4 0 0 0 0 5 182 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 1 203 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 214 3 0.0 4 0 0 0 0 3 215 1 0.0 4 0 0 0 1 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 1 1 237 7 0.0 4 0 0 0 0 7 239 1 0.0 4 0 0 0 0 1 254 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 1 2 265 3 0.0 4 0 0 0 0 3 268 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.79 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 80,174 Reads with adapters: 5,239 (6.5%) Reads written (passing filters): 80,174 (100.0%) Total basepairs processed: 21,924,175 bp Total written (filtered): 21,836,636 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5239 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.9% C: 12.0% G: 77.6% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 276 1252.7 0 276 4 27 313.2 0 27 5 2 78.3 0 2 6 1 19.6 0 1 8 1 1.2 0 1 9 115 0.3 0 14 101 10 292 0.1 1 29 263 11 3654 0.0 1 179 3475 12 605 0.0 1 18 587 13 83 0.0 1 5 78 14 7 0.0 1 0 7 15 5 0.0 1 0 5 16 3 0.0 1 1 2 20 1 0.0 1 1 74 1 0.0 1 1 108 1 0.0 1 1 150 2 0.0 1 2 152 16 0.0 1 12 4 153 4 0.0 1 4 191 4 0.0 1 0 4 210 114 0.0 1 89 25 211 17 0.0 1 13 4 225 1 0.0 1 1 230 1 0.0 1 1 231 1 0.0 1 1 269 3 0.0 1 2 1 270 2 0.0 1 2