This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.78 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 411,828 (14.4%) Reads that were too short: 12,368 (0.4%) Reads written (passing filters): 399,460 (14.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 97,795,460 bp (11.4%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 411828 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5659 696.4 0 5659 7 86433 696.4 0 86433 8 1692 696.4 0 1692 9 78029 696.4 0 78029 10 108 696.4 0 108 11 60 696.4 0 60 12 63 696.4 0 63 13 57 696.4 0 57 14 41 696.4 0 41 15 64 696.4 0 64 16 67 696.4 0 67 17 63 696.4 0 63 18 80 696.4 0 80 19 61 696.4 0 61 20 68 696.4 0 68 21 63 696.4 0 63 22 71 696.4 0 71 23 46 696.4 0 46 24 83 696.4 0 83 25 51 696.4 0 51 26 59 696.4 0 59 27 57 696.4 0 57 28 47 696.4 0 47 29 47 696.4 0 47 30 52 696.4 0 52 31 43 696.4 0 43 32 55 696.4 0 55 33 52 696.4 0 52 34 42 696.4 0 42 35 70 696.4 0 70 36 59 696.4 0 59 37 60 696.4 0 60 38 38 696.4 0 38 39 57 696.4 0 57 40 61 696.4 0 61 41 54 696.4 0 54 42 66 696.4 0 66 43 67 696.4 0 67 44 65 696.4 0 65 45 78 696.4 0 78 46 66 696.4 0 66 47 66 696.4 0 66 48 46 696.4 0 46 49 64 696.4 0 64 50 40 696.4 0 40 51 78 696.4 0 78 52 69 696.4 0 69 53 81 696.4 0 81 54 100 696.4 0 100 55 97 696.4 0 97 56 132 696.4 0 132 57 102 696.4 0 102 58 69 696.4 0 69 59 54 696.4 0 54 60 89 696.4 0 89 61 92 696.4 0 92 62 151 696.4 0 151 63 100 696.4 0 100 64 81 696.4 0 81 65 81 696.4 0 81 66 156 696.4 0 156 67 89 696.4 0 89 68 164 696.4 0 164 69 207 696.4 0 207 70 253 696.4 0 253 71 308 696.4 0 308 72 685 696.4 0 685 73 1460 696.4 0 1460 74 439 696.4 0 439 75 491 696.4 0 491 76 1138 696.4 0 1138 77 382 696.4 0 382 78 421 696.4 0 421 79 962 696.4 0 962 80 952 696.4 0 952 81 1436 696.4 0 1436 82 896 696.4 0 896 83 1604 696.4 0 1604 84 1784 696.4 0 1784 85 3765 696.4 0 3765 86 72403 696.4 0 72403 87 52005 696.4 0 52005 88 57613 696.4 0 57613 89 246 696.4 0 246 90 63 696.4 0 63 91 36 696.4 0 36 92 29 696.4 0 29 93 37 696.4 0 37 94 41 696.4 0 41 95 43 696.4 0 43 96 82 696.4 0 82 97 28 696.4 0 28 98 49 696.4 0 49 99 72 696.4 0 72 100 58 696.4 0 58 101 57 696.4 0 57 102 53 696.4 0 53 103 91 696.4 0 91 104 42 696.4 0 42 105 68 696.4 0 68 106 39 696.4 0 39 107 30 696.4 0 30 108 58 696.4 0 58 109 34 696.4 0 34 110 48 696.4 0 48 111 56 696.4 0 56 112 52 696.4 0 52 113 43 696.4 0 43 114 46 696.4 0 46 115 34 696.4 0 34 116 49 696.4 0 49 117 57 696.4 0 57 118 49 696.4 0 49 119 52 696.4 0 52 120 62 696.4 0 62 121 61 696.4 0 61 122 61 696.4 0 61 123 232 696.4 0 232 124 375 696.4 0 375 125 300 696.4 0 300 126 425 696.4 0 425 127 150 696.4 0 150 128 61 696.4 0 61 129 172 696.4 0 172 130 94 696.4 0 94 131 109 696.4 0 109 132 58 696.4 0 58 133 75 696.4 0 75 134 108 696.4 0 108 135 76 696.4 0 76 136 101 696.4 0 101 137 105 696.4 0 105 138 90 696.4 0 90 139 128 696.4 0 128 140 86 696.4 0 86 141 184 696.4 0 184 142 117 696.4 0 117 143 208 696.4 0 208 144 3222 696.4 0 3222 145 2648 696.4 0 2648 146 2666 696.4 0 2666 147 88 696.4 0 88 148 62 696.4 0 62 149 85 696.4 0 85 150 63 696.4 0 63 151 83 696.4 0 83 152 42 696.4 0 42 153 55 696.4 0 55 154 60 696.4 0 60 155 64 696.4 0 64 156 66 696.4 0 66 157 80 696.4 0 80 158 67 696.4 0 67 159 63 696.4 0 63 160 63 696.4 0 63 161 80 696.4 0 80 162 54 696.4 0 54 163 84 696.4 0 84 164 79 696.4 0 79 165 117 696.4 0 117 166 1156 696.4 0 1156 167 796 696.4 0 796 168 992 696.4 0 992 169 63 696.4 0 63 170 60 696.4 0 60 171 67 696.4 0 67 172 108 696.4 0 108 173 62 696.4 0 62 174 61 696.4 0 61 175 81 696.4 0 81 176 77 696.4 0 77 177 64 696.4 0 64 178 78 696.4 0 78 179 70 696.4 0 70 180 96 696.4 0 96 181 83 696.4 0 83 182 94 696.4 0 94 183 107 696.4 0 107 184 136 696.4 0 136 185 2716 696.4 0 2716 186 655 696.4 0 655 187 447 696.4 0 447 188 78 696.4 0 78 189 101 696.4 0 101 190 52 696.4 0 52 191 53 696.4 0 53 192 65 696.4 0 65 193 102 696.4 0 102 194 71 696.4 0 71 195 77 696.4 0 77 196 79 696.4 0 79 197 64 696.4 0 64 198 66 696.4 0 66 199 82 696.4 0 82 200 87 696.4 0 87 201 84 696.4 0 84 202 79 696.4 0 79 203 114 696.4 0 114 204 134 696.4 0 134 205 159 696.4 0 159 206 2247 696.4 0 2247 207 1253 696.4 0 1253 208 1654 696.4 0 1654 209 71 696.4 0 71 210 53 696.4 0 53 211 404 696.4 0 404 212 288 696.4 0 288 213 293 696.4 0 293 214 73 696.4 0 73 215 41 696.4 0 41 216 54 696.4 0 54 217 57 696.4 0 57 218 41 696.4 0 41 219 39 696.4 0 39 220 52 696.4 0 52 221 63 696.4 0 63 222 73 696.4 0 73 223 49 696.4 0 49 224 49 696.4 0 49 225 66 696.4 0 66 226 82 696.4 0 82 227 76 696.4 0 76 228 128 696.4 0 128 229 59 696.4 0 59 230 42 696.4 0 42 231 51 696.4 0 51 232 57 696.4 0 57 233 35 696.4 0 35 234 44 696.4 0 44 235 40 696.4 0 40 236 35 696.4 0 35 237 49 696.4 0 49 238 58 696.4 0 58 239 44 696.4 0 44 240 62 696.4 0 62 241 64 696.4 0 64 242 60 696.4 0 60 243 74 696.4 0 74 244 37 696.4 0 37 245 71 696.4 0 71 246 68 696.4 0 68 247 52 696.4 0 52 248 66 696.4 0 66 249 52 696.4 0 52 250 35 696.4 0 35 251 42 696.4 0 42 252 30 696.4 0 30 253 40 696.4 0 40 254 52 696.4 0 52 255 72 696.4 0 72 256 58 696.4 0 58 257 73 696.4 0 73 258 43 696.4 0 43 259 149 696.4 0 149 260 104 696.4 0 104 261 231 696.4 0 231 262 104 696.4 0 104 263 46 696.4 0 46 264 54 696.4 0 54 265 40 696.4 0 40 266 57 696.4 0 57 267 96 696.4 0 96 268 107 696.4 0 107 269 86 696.4 0 86 270 57 696.4 0 57 271 54 696.4 0 54 272 57 696.4 0 57 273 49 696.4 0 49 274 51 696.4 0 51 275 116 696.4 0 116 276 66 696.4 0 66 277 62 696.4 0 62 278 36 696.4 0 36 279 79 696.4 0 79 280 68 696.4 0 68 281 87 696.4 0 87 282 104 696.4 0 104 283 56 696.4 0 56 284 47 696.4 0 47 285 42 696.4 0 42 286 36 696.4 0 36 287 56 696.4 0 56 288 53 696.4 0 53 289 56 696.4 0 56 290 36 696.4 0 36 291 41 696.4 0 41 292 42 696.4 0 42 293 56 696.4 0 56 294 92 696.4 0 92 295 101 696.4 0 101 296 91 696.4 0 91 297 83 696.4 0 83 298 47 696.4 0 47 299 46 696.4 0 46 300 62 696.4 0 62 301 108 696.4 0 108 Finished in 15.86 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 399,460 Reads with adapters: 84,185 (21.1%) Reads that were too short: 173 (0.0%) Reads written (passing filters): 84,012 (21.0%) Total basepairs processed: 97,795,460 bp Total written (filtered): 22,821,260 bp (23.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84185 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 15 0.0 3 0 0 15 16 433 0.0 3 0 425 6 2 17 79410 0.0 3 77893 1457 51 9 18 214 0.0 3 12 194 7 1 19 2109 0.0 3 2031 47 27 4 20 81 0.0 3 1 5 0 75 21 1 0.0 3 0 0 0 1 22 3 0.0 3 2 0 0 1 29 1 0.0 3 1 30 1 0.0 3 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 4 0.0 3 0 0 0 4 69 1 0.0 3 0 0 0 1 80 13 0.0 3 0 0 0 13 82 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 85 3 0.0 3 0 0 0 3 86 1 0.0 3 1 88 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 2 0.0 3 0 0 0 2 101 17 0.0 3 0 0 0 17 104 57 0.0 3 0 0 0 57 105 1466 0.0 3 0 0 3 1463 106 21 0.0 3 0 0 0 21 107 1 0.0 3 0 0 0 1 108 127 0.0 3 0 0 5 122 111 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 10 0.0 3 0 0 0 10 158 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 5 0.0 3 0 0 0 5 165 4 0.0 3 0 0 0 4 166 8 0.0 3 0 0 0 8 167 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 4 0.0 3 0 0 0 4 196 3 0.0 3 0 0 1 2 197 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 60 0.0 3 0 0 1 59 207 18 0.0 3 0 0 1 17 208 3 0.0 3 0 0 0 3 217 20 0.0 3 0 0 1 19 218 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 223 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 5 0.0 3 0 0 0 5 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.00 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 84,012 Reads with adapters: 5,585 (6.6%) Reads written (passing filters): 84,012 (100.0%) Total basepairs processed: 22,821,260 bp Total written (filtered): 22,804,333 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5585 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.7% C: 7.3% G: 13.3% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5437 1312.7 0 5437 4 137 328.2 0 137 5 8 82.0 0 8 7 1 5.1 0 1 9 1 0.3 0 0 1 12 1 0.0 1 0 1