This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.06 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 140,030 (4.9%) Reads that were too short: 36,135 (1.3%) Reads written (passing filters): 103,895 (3.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 25,857,620 bp (3.0%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 140030 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 19377 696.4 0 19377 7 11316 696.4 0 11316 8 15793 696.4 0 15793 9 14010 696.4 0 14010 10 118 696.4 0 118 11 120 696.4 0 120 12 72 696.4 0 72 13 80 696.4 0 80 14 74 696.4 0 74 15 107 696.4 0 107 16 85 696.4 0 85 17 73 696.4 0 73 18 81 696.4 0 81 19 89 696.4 0 89 20 75 696.4 0 75 21 73 696.4 0 73 22 72 696.4 0 72 23 88 696.4 0 88 24 97 696.4 0 97 25 73 696.4 0 73 26 85 696.4 0 85 27 139 696.4 0 139 28 120 696.4 0 120 29 66 696.4 0 66 30 90 696.4 0 90 31 81 696.4 0 81 32 109 696.4 0 109 33 113 696.4 0 113 34 80 696.4 0 80 35 132 696.4 0 132 36 150 696.4 0 150 37 1090 696.4 0 1090 38 1471 696.4 0 1471 39 1694 696.4 0 1694 40 290 696.4 0 290 41 95 696.4 0 95 42 75 696.4 0 75 43 110 696.4 0 110 44 113 696.4 0 113 45 92 696.4 0 92 46 82 696.4 0 82 47 99 696.4 0 99 48 98 696.4 0 98 49 94 696.4 0 94 50 82 696.4 0 82 51 96 696.4 0 96 52 69 696.4 0 69 53 110 696.4 0 110 54 92 696.4 0 92 55 86 696.4 0 86 56 85 696.4 0 85 57 95 696.4 0 95 58 87 696.4 0 87 59 92 696.4 0 92 60 69 696.4 0 69 61 69 696.4 0 69 62 92 696.4 0 92 63 65 696.4 0 65 64 62 696.4 0 62 65 77 696.4 0 77 66 86 696.4 0 86 67 89 696.4 0 89 68 87 696.4 0 87 69 98 696.4 0 98 70 116 696.4 0 116 71 73 696.4 0 73 72 96 696.4 0 96 73 86 696.4 0 86 74 104 696.4 0 104 75 281 696.4 0 281 76 200 696.4 0 200 77 100 696.4 0 100 78 133 696.4 0 133 79 136 696.4 0 136 80 129 696.4 0 129 81 117 696.4 0 117 82 104 696.4 0 104 83 92 696.4 0 92 84 87 696.4 0 87 85 65 696.4 0 65 86 86 696.4 0 86 87 69 696.4 0 69 88 95 696.4 0 95 89 99 696.4 0 99 90 185 696.4 0 185 91 255 696.4 0 255 92 562 696.4 0 562 93 501 696.4 0 501 94 325 696.4 0 325 95 205 696.4 0 205 96 86 696.4 0 86 97 104 696.4 0 104 98 87 696.4 0 87 99 65 696.4 0 65 100 77 696.4 0 77 101 85 696.4 0 85 102 74 696.4 0 74 103 95 696.4 0 95 104 90 696.4 0 90 105 79 696.4 0 79 106 85 696.4 0 85 107 93 696.4 0 93 108 91 696.4 0 91 109 111 696.4 0 111 110 117 696.4 0 117 111 81 696.4 0 81 112 242 696.4 0 242 113 206 696.4 0 206 114 246 696.4 0 246 115 173 696.4 0 173 116 112 696.4 0 112 117 114 696.4 0 114 118 117 696.4 0 117 119 90 696.4 0 90 120 103 696.4 0 103 121 84 696.4 0 84 122 122 696.4 0 122 123 105 696.4 0 105 124 100 696.4 0 100 125 112 696.4 0 112 126 103 696.4 0 103 127 131 696.4 0 131 128 170 696.4 0 170 129 336 696.4 0 336 130 484 696.4 0 484 131 289 696.4 0 289 132 176 696.4 0 176 133 166 696.4 0 166 134 145 696.4 0 145 135 161 696.4 0 161 136 213 696.4 0 213 137 217 696.4 0 217 138 175 696.4 0 175 139 224 696.4 0 224 140 296 696.4 0 296 141 451 696.4 0 451 142 4377 696.4 0 4377 143 6369 696.4 0 6369 144 6229 696.4 0 6229 145 108 696.4 0 108 146 64 696.4 0 64 147 91 696.4 0 91 148 116 696.4 0 116 149 241 696.4 0 241 150 224 696.4 0 224 151 231 696.4 0 231 152 68 696.4 0 68 153 85 696.4 0 85 154 76 696.4 0 76 155 87 696.4 0 87 156 94 696.4 0 94 157 108 696.4 0 108 158 81 696.4 0 81 159 82 696.4 0 82 160 78 696.4 0 78 161 58 696.4 0 58 162 83 696.4 0 83 163 78 696.4 0 78 164 89 696.4 0 89 165 90 696.4 0 90 166 84 696.4 0 84 167 78 696.4 0 78 168 76 696.4 0 76 169 105 696.4 0 105 170 107 696.4 0 107 171 92 696.4 0 92 172 72 696.4 0 72 173 103 696.4 0 103 174 83 696.4 0 83 175 88 696.4 0 88 176 82 696.4 0 82 177 114 696.4 0 114 178 137 696.4 0 137 179 140 696.4 0 140 180 82 696.4 0 82 181 52 696.4 0 52 182 215 696.4 0 215 183 75 696.4 0 75 184 73 696.4 0 73 185 69 696.4 0 69 186 72 696.4 0 72 187 63 696.4 0 63 188 81 696.4 0 81 189 85 696.4 0 85 190 96 696.4 0 96 191 74 696.4 0 74 192 78 696.4 0 78 193 61 696.4 0 61 194 70 696.4 0 70 195 64 696.4 0 64 196 67 696.4 0 67 197 85 696.4 0 85 198 92 696.4 0 92 199 71 696.4 0 71 200 85 696.4 0 85 201 104 696.4 0 104 202 104 696.4 0 104 203 125 696.4 0 125 204 121 696.4 0 121 205 101 696.4 0 101 206 72 696.4 0 72 207 89 696.4 0 89 208 126 696.4 0 126 209 506 696.4 0 506 210 261 696.4 0 261 211 361 696.4 0 361 212 199 696.4 0 199 213 256 696.4 0 256 214 1649 696.4 0 1649 215 1069 696.4 0 1069 216 2663 696.4 0 2663 217 336 696.4 0 336 218 255 696.4 0 255 219 82 696.4 0 82 220 92 696.4 0 92 221 142 696.4 0 142 222 115 696.4 0 115 223 135 696.4 0 135 224 150 696.4 0 150 225 91 696.4 0 91 226 154 696.4 0 154 227 192 696.4 0 192 228 241 696.4 0 241 229 696 696.4 0 696 230 453 696.4 0 453 231 665 696.4 0 665 232 323 696.4 0 323 233 470 696.4 0 470 234 4311 696.4 0 4311 235 4547 696.4 0 4547 236 5261 696.4 0 5261 237 1327 696.4 0 1327 238 79 696.4 0 79 239 68 696.4 0 68 240 70 696.4 0 70 241 81 696.4 0 81 242 69 696.4 0 69 243 86 696.4 0 86 244 83 696.4 0 83 245 66 696.4 0 66 246 67 696.4 0 67 247 78 696.4 0 78 248 87 696.4 0 87 249 71 696.4 0 71 250 73 696.4 0 73 251 73 696.4 0 73 252 88 696.4 0 88 253 238 696.4 0 238 254 291 696.4 0 291 255 305 696.4 0 305 256 244 696.4 0 244 257 81 696.4 0 81 258 78 696.4 0 78 259 81 696.4 0 81 260 101 696.4 0 101 261 107 696.4 0 107 262 362 696.4 0 362 263 270 696.4 0 270 264 235 696.4 0 235 265 103 696.4 0 103 266 55 696.4 0 55 267 66 696.4 0 66 268 47 696.4 0 47 269 66 696.4 0 66 270 112 696.4 0 112 271 88 696.4 0 88 272 119 696.4 0 119 273 70 696.4 0 70 274 58 696.4 0 58 275 85 696.4 0 85 276 118 696.4 0 118 277 144 696.4 0 144 278 155 696.4 0 155 279 91 696.4 0 91 280 63 696.4 0 63 281 78 696.4 0 78 282 100 696.4 0 100 283 75 696.4 0 75 284 151 696.4 0 151 285 111 696.4 0 111 286 74 696.4 0 74 287 51 696.4 0 51 288 49 696.4 0 49 289 58 696.4 0 58 290 43 696.4 0 43 291 93 696.4 0 93 292 84 696.4 0 84 293 114 696.4 0 114 294 65 696.4 0 65 295 202 696.4 0 202 296 429 696.4 0 429 297 693 696.4 0 693 298 435 696.4 0 435 299 342 696.4 0 342 300 52 696.4 0 52 301 124 696.4 0 124 Finished in 5.15 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 103,895 Reads with adapters: 27,112 (26.1%) Reads that were too short: 67 (0.1%) Reads written (passing filters): 27,045 (26.0%) Total basepairs processed: 25,857,620 bp Total written (filtered): 7,325,970 bp (28.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27112 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 12 0.0 3 0 0 5 3 4 19 135 0.0 3 0 111 8 13 3 20 14697 0.0 4 13660 806 159 55 17 21 10832 0.0 4 10088 606 107 22 9 22 60 0.0 4 13 37 7 1 2 23 858 0.0 4 727 46 11 55 19 24 41 0.0 4 2 2 0 23 14 26 4 0.0 4 0 0 0 3 1 29 1 0.0 4 1 42 78 0.0 4 0 0 0 0 78 45 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 58 1 0.0 4 1 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 70 3 0.0 4 0 0 0 0 3 84 1 0.0 4 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 97 113 0.0 4 0 0 0 1 112 98 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 93 0.0 4 0 0 0 0 93 128 97 0.0 4 0 0 0 0 97 136 2 0.0 4 0 0 0 0 2 140 1 0.0 4 1 148 1 0.0 4 0 0 0 0 1 154 3 0.0 4 0 0 0 0 3 156 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 1 172 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 1 178 1 0.0 4 0 0 0 0 1 188 17 0.0 4 0 0 0 0 17 198 2 0.0 4 0 0 0 0 2 199 2 0.0 4 0 0 0 0 2 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 229 3 0.0 4 0 0 0 0 3 230 3 0.0 4 0 0 0 1 2 234 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 237 2 0.0 4 0 0 0 0 2 238 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 261 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 1 295 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.99 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 27,045 Reads with adapters: 5,667 (21.0%) Reads written (passing filters): 27,045 (100.0%) Total basepairs processed: 7,325,970 bp Total written (filtered): 7,262,450 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5667 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 9.0% G: 85.2% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 51 422.6 0 51 8 3 0.4 0 3 9 810 0.1 0 101 709 10 2871 0.0 1 244 2627 11 1832 0.0 1 87 1745 12 50 0.0 1 1 49 13 13 0.0 1 0 13 14 1 0.0 1 0 1 15 2 0.0 1 0 2 22 1 0.0 1 0 1 150 8 0.0 1 8 151 6 0.0 1 6 153 1 0.0 1 1 208 2 0.0 1 2 209 9 0.0 1 8 1 224 2 0.0 1 2 270 4 0.0 1 3 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.