This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.75 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 118,403 (4.2%) Reads that were too short: 19,496 (0.7%) Reads written (passing filters): 98,907 (3.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,901,433 bp (2.9%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 118403 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 18134 696.4 0 18134 7 12918 696.4 0 12918 8 15692 696.4 0 15692 9 15497 696.4 0 15497 10 74 696.4 0 74 11 82 696.4 0 82 12 44 696.4 0 44 13 54 696.4 0 54 14 53 696.4 0 53 15 46 696.4 0 46 16 54 696.4 0 54 17 73 696.4 0 73 18 55 696.4 0 55 19 74 696.4 0 74 20 58 696.4 0 58 21 68 696.4 0 68 22 46 696.4 0 46 23 59 696.4 0 59 24 83 696.4 0 83 25 52 696.4 0 52 26 83 696.4 0 83 27 68 696.4 0 68 28 79 696.4 0 79 29 60 696.4 0 60 30 69 696.4 0 69 31 68 696.4 0 68 32 64 696.4 0 64 33 80 696.4 0 80 34 84 696.4 0 84 35 90 696.4 0 90 36 80 696.4 0 80 37 723 696.4 0 723 38 994 696.4 0 994 39 1011 696.4 0 1011 40 66 696.4 0 66 41 72 696.4 0 72 42 75 696.4 0 75 43 69 696.4 0 69 44 80 696.4 0 80 45 75 696.4 0 75 46 73 696.4 0 73 47 69 696.4 0 69 48 69 696.4 0 69 49 70 696.4 0 70 50 59 696.4 0 59 51 64 696.4 0 64 52 58 696.4 0 58 53 72 696.4 0 72 54 67 696.4 0 67 55 52 696.4 0 52 56 63 696.4 0 63 57 67 696.4 0 67 58 77 696.4 0 77 59 55 696.4 0 55 60 53 696.4 0 53 61 61 696.4 0 61 62 67 696.4 0 67 63 70 696.4 0 70 64 61 696.4 0 61 65 75 696.4 0 75 66 53 696.4 0 53 67 57 696.4 0 57 68 63 696.4 0 63 69 70 696.4 0 70 70 72 696.4 0 72 71 72 696.4 0 72 72 64 696.4 0 64 73 58 696.4 0 58 74 78 696.4 0 78 75 248 696.4 0 248 76 177 696.4 0 177 77 59 696.4 0 59 78 91 696.4 0 91 79 56 696.4 0 56 80 83 696.4 0 83 81 90 696.4 0 90 82 54 696.4 0 54 83 69 696.4 0 69 84 43 696.4 0 43 85 49 696.4 0 49 86 48 696.4 0 48 87 52 696.4 0 52 88 61 696.4 0 61 89 48 696.4 0 48 90 117 696.4 0 117 91 152 696.4 0 152 92 273 696.4 0 273 93 337 696.4 0 337 94 152 696.4 0 152 95 145 696.4 0 145 96 65 696.4 0 65 97 67 696.4 0 67 98 53 696.4 0 53 99 65 696.4 0 65 100 65 696.4 0 65 101 53 696.4 0 53 102 58 696.4 0 58 103 75 696.4 0 75 104 82 696.4 0 82 105 52 696.4 0 52 106 70 696.4 0 70 107 74 696.4 0 74 108 65 696.4 0 65 109 65 696.4 0 65 110 81 696.4 0 81 111 59 696.4 0 59 112 186 696.4 0 186 113 187 696.4 0 187 114 130 696.4 0 130 115 113 696.4 0 113 116 112 696.4 0 112 117 89 696.4 0 89 118 94 696.4 0 94 119 53 696.4 0 53 120 57 696.4 0 57 121 65 696.4 0 65 122 79 696.4 0 79 123 76 696.4 0 76 124 55 696.4 0 55 125 68 696.4 0 68 126 95 696.4 0 95 127 125 696.4 0 125 128 133 696.4 0 133 129 263 696.4 0 263 130 497 696.4 0 497 131 247 696.4 0 247 132 193 696.4 0 193 133 116 696.4 0 116 134 107 696.4 0 107 135 143 696.4 0 143 136 185 696.4 0 185 137 174 696.4 0 174 138 142 696.4 0 142 139 189 696.4 0 189 140 217 696.4 0 217 141 352 696.4 0 352 142 4602 696.4 0 4602 143 6698 696.4 0 6698 144 6801 696.4 0 6801 145 91 696.4 0 91 146 53 696.4 0 53 147 61 696.4 0 61 148 57 696.4 0 57 149 134 696.4 0 134 150 145 696.4 0 145 151 154 696.4 0 154 152 63 696.4 0 63 153 69 696.4 0 69 154 48 696.4 0 48 155 61 696.4 0 61 156 58 696.4 0 58 157 59 696.4 0 59 158 60 696.4 0 60 159 51 696.4 0 51 160 40 696.4 0 40 161 49 696.4 0 49 162 53 696.4 0 53 163 72 696.4 0 72 164 66 696.4 0 66 165 52 696.4 0 52 166 62 696.4 0 62 167 40 696.4 0 40 168 56 696.4 0 56 169 72 696.4 0 72 170 78 696.4 0 78 171 72 696.4 0 72 172 56 696.4 0 56 173 71 696.4 0 71 174 53 696.4 0 53 175 59 696.4 0 59 176 66 696.4 0 66 177 61 696.4 0 61 178 94 696.4 0 94 179 93 696.4 0 93 180 67 696.4 0 67 181 43 696.4 0 43 182 220 696.4 0 220 183 55 696.4 0 55 184 51 696.4 0 51 185 51 696.4 0 51 186 44 696.4 0 44 187 46 696.4 0 46 188 41 696.4 0 41 189 51 696.4 0 51 190 50 696.4 0 50 191 52 696.4 0 52 192 56 696.4 0 56 193 50 696.4 0 50 194 56 696.4 0 56 195 50 696.4 0 50 196 47 696.4 0 47 197 45 696.4 0 45 198 55 696.4 0 55 199 35 696.4 0 35 200 43 696.4 0 43 201 79 696.4 0 79 202 76 696.4 0 76 203 72 696.4 0 72 204 58 696.4 0 58 205 76 696.4 0 76 206 46 696.4 0 46 207 57 696.4 0 57 208 77 696.4 0 77 209 214 696.4 0 214 210 121 696.4 0 121 211 166 696.4 0 166 212 90 696.4 0 90 213 117 696.4 0 117 214 594 696.4 0 594 215 463 696.4 0 463 216 946 696.4 0 946 217 174 696.4 0 174 218 109 696.4 0 109 219 42 696.4 0 42 220 73 696.4 0 73 221 84 696.4 0 84 222 71 696.4 0 71 223 71 696.4 0 71 224 74 696.4 0 74 225 74 696.4 0 74 226 97 696.4 0 97 227 118 696.4 0 118 228 150 696.4 0 150 229 461 696.4 0 461 230 259 696.4 0 259 231 406 696.4 0 406 232 151 696.4 0 151 233 201 696.4 0 201 234 1886 696.4 0 1886 235 2515 696.4 0 2515 236 2674 696.4 0 2674 237 952 696.4 0 952 238 67 696.4 0 67 239 49 696.4 0 49 240 49 696.4 0 49 241 65 696.4 0 65 242 39 696.4 0 39 243 54 696.4 0 54 244 58 696.4 0 58 245 55 696.4 0 55 246 36 696.4 0 36 247 55 696.4 0 55 248 41 696.4 0 41 249 51 696.4 0 51 250 50 696.4 0 50 251 52 696.4 0 52 252 37 696.4 0 37 253 89 696.4 0 89 254 115 696.4 0 115 255 127 696.4 0 127 256 101 696.4 0 101 257 27 696.4 0 27 258 55 696.4 0 55 259 45 696.4 0 45 260 57 696.4 0 57 261 82 696.4 0 82 262 503 696.4 0 503 263 265 696.4 0 265 264 245 696.4 0 245 265 71 696.4 0 71 266 44 696.4 0 44 267 48 696.4 0 48 268 66 696.4 0 66 269 45 696.4 0 45 270 81 696.4 0 81 271 62 696.4 0 62 272 81 696.4 0 81 273 40 696.4 0 40 274 43 696.4 0 43 275 46 696.4 0 46 276 79 696.4 0 79 277 122 696.4 0 122 278 134 696.4 0 134 279 93 696.4 0 93 280 57 696.4 0 57 281 57 696.4 0 57 282 47 696.4 0 47 283 57 696.4 0 57 284 63 696.4 0 63 285 74 696.4 0 74 286 55 696.4 0 55 287 47 696.4 0 47 288 47 696.4 0 47 289 31 696.4 0 31 290 44 696.4 0 44 291 84 696.4 0 84 292 58 696.4 0 58 293 87 696.4 0 87 294 59 696.4 0 59 295 137 696.4 0 137 296 294 696.4 0 294 297 396 696.4 0 396 298 222 696.4 0 222 299 116 696.4 0 116 300 32 696.4 0 32 301 93 696.4 0 93 Finished in 4.33 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 98,907 Reads with adapters: 31,868 (32.2%) Reads that were too short: 47 (0.0%) Reads written (passing filters): 31,821 (32.2%) Total basepairs processed: 24,901,433 bp Total written (filtered): 8,795,459 bp (35.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31868 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 105 0.0 3 0 101 4 17 15709 0.0 3 15227 465 15 2 18 15862 0.0 3 15486 366 10 19 67 0.0 3 9 47 10 1 20 29 0.0 3 4 0 4 21 21 20 0.0 3 0 0 0 20 22 1 0.0 3 0 0 0 1 27 2 0.0 3 2 32 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 45 3 0.0 3 0 0 0 3 50 8 0.0 3 0 0 0 8 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 0 25 256 2 0.0 3 0 0 0 2 262 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.12 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 31,821 Reads with adapters: 152 (0.5%) Reads written (passing filters): 31,821 (100.0%) Total basepairs processed: 8,795,459 bp Total written (filtered): 8,794,941 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 152 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.3% C: 10.5% G: 29.6% T: 4.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 95 497.2 0 95 4 53 124.3 0 53 5 3 31.1 0 3 6 1 7.8 0 1