This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.93 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 199,585 (7.0%) Reads that were too short: 12,732 (0.4%) Reads written (passing filters): 186,853 (6.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 52,986,631 bp (6.2%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 199585 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4225 696.4 0 4225 7 91474 696.4 0 91474 8 74525 696.4 0 74525 9 114 696.4 0 114 10 24 696.4 0 24 11 26 696.4 0 26 12 35 696.4 0 35 13 34 696.4 0 34 14 25 696.4 0 25 15 15 696.4 0 15 16 29 696.4 0 29 17 27 696.4 0 27 18 47 696.4 0 47 19 28 696.4 0 28 20 71 696.4 0 71 21 49 696.4 0 49 22 40 696.4 0 40 23 23 696.4 0 23 24 27 696.4 0 27 25 35 696.4 0 35 26 30 696.4 0 30 27 84 696.4 0 84 28 32 696.4 0 32 29 200 696.4 0 200 30 56 696.4 0 56 31 158 696.4 0 158 32 29 696.4 0 29 33 37 696.4 0 37 34 26 696.4 0 26 35 27 696.4 0 27 36 18 696.4 0 18 37 32 696.4 0 32 38 20 696.4 0 20 39 30 696.4 0 30 40 46 696.4 0 46 41 40 696.4 0 40 42 25 696.4 0 25 43 30 696.4 0 30 44 30 696.4 0 30 45 38 696.4 0 38 46 39 696.4 0 39 47 44 696.4 0 44 48 41 696.4 0 41 49 24 696.4 0 24 50 25 696.4 0 25 51 18 696.4 0 18 52 48 696.4 0 48 53 34 696.4 0 34 54 117 696.4 0 117 55 426 696.4 0 426 56 105 696.4 0 105 57 22 696.4 0 22 58 37 696.4 0 37 59 35 696.4 0 35 60 33 696.4 0 33 61 188 696.4 0 188 62 175 696.4 0 175 63 247 696.4 0 247 64 37 696.4 0 37 65 53 696.4 0 53 66 31 696.4 0 31 67 103 696.4 0 103 68 87 696.4 0 87 69 109 696.4 0 109 70 62 696.4 0 62 71 198 696.4 0 198 72 73 696.4 0 73 73 63 696.4 0 63 74 61 696.4 0 61 75 37 696.4 0 37 76 26 696.4 0 26 77 47 696.4 0 47 78 48 696.4 0 48 79 73 696.4 0 73 80 67 696.4 0 67 81 61 696.4 0 61 82 61 696.4 0 61 83 129 696.4 0 129 84 99 696.4 0 99 85 64 696.4 0 64 86 89 696.4 0 89 87 116 696.4 0 116 88 58 696.4 0 58 89 51 696.4 0 51 90 45 696.4 0 45 91 39 696.4 0 39 92 56 696.4 0 56 93 29 696.4 0 29 94 36 696.4 0 36 95 50 696.4 0 50 96 55 696.4 0 55 97 48 696.4 0 48 98 60 696.4 0 60 99 31 696.4 0 31 100 30 696.4 0 30 101 17 696.4 0 17 102 23 696.4 0 23 103 28 696.4 0 28 104 22 696.4 0 22 105 27 696.4 0 27 106 23 696.4 0 23 107 32 696.4 0 32 108 38 696.4 0 38 109 27 696.4 0 27 110 29 696.4 0 29 111 34 696.4 0 34 112 56 696.4 0 56 113 50 696.4 0 50 114 54 696.4 0 54 115 63 696.4 0 63 116 33 696.4 0 33 117 41 696.4 0 41 118 32 696.4 0 32 119 55 696.4 0 55 120 56 696.4 0 56 121 38 696.4 0 38 122 63 696.4 0 63 123 145 696.4 0 145 124 122 696.4 0 122 125 190 696.4 0 190 126 431 696.4 0 431 127 340 696.4 0 340 128 948 696.4 0 948 129 657 696.4 0 657 130 587 696.4 0 587 131 221 696.4 0 221 132 97 696.4 0 97 133 58 696.4 0 58 134 149 696.4 0 149 135 102 696.4 0 102 136 119 696.4 0 119 137 29 696.4 0 29 138 68 696.4 0 68 139 56 696.4 0 56 140 383 696.4 0 383 141 42 696.4 0 42 142 37 696.4 0 37 143 100 696.4 0 100 144 52 696.4 0 52 145 89 696.4 0 89 146 75 696.4 0 75 147 60 696.4 0 60 148 76 696.4 0 76 149 62 696.4 0 62 150 63 696.4 0 63 151 52 696.4 0 52 152 39 696.4 0 39 153 43 696.4 0 43 154 39 696.4 0 39 155 52 696.4 0 52 156 31 696.4 0 31 157 35 696.4 0 35 158 32 696.4 0 32 159 59 696.4 0 59 160 43 696.4 0 43 161 46 696.4 0 46 162 55 696.4 0 55 163 40 696.4 0 40 164 49 696.4 0 49 165 76 696.4 0 76 166 404 696.4 0 404 167 232 696.4 0 232 168 213 696.4 0 213 169 58 696.4 0 58 170 39 696.4 0 39 171 50 696.4 0 50 172 43 696.4 0 43 173 51 696.4 0 51 174 65 696.4 0 65 175 75 696.4 0 75 176 71 696.4 0 71 177 52 696.4 0 52 178 35 696.4 0 35 179 31 696.4 0 31 180 50 696.4 0 50 181 28 696.4 0 28 182 62 696.4 0 62 183 64 696.4 0 64 184 71 696.4 0 71 185 68 696.4 0 68 186 68 696.4 0 68 187 62 696.4 0 62 188 50 696.4 0 50 189 54 696.4 0 54 190 63 696.4 0 63 191 61 696.4 0 61 192 84 696.4 0 84 193 68 696.4 0 68 194 83 696.4 0 83 195 89 696.4 0 89 196 209 696.4 0 209 197 489 696.4 0 489 198 398 696.4 0 398 199 399 696.4 0 399 200 70 696.4 0 70 201 53 696.4 0 53 202 66 696.4 0 66 203 57 696.4 0 57 204 38 696.4 0 38 205 61 696.4 0 61 206 63 696.4 0 63 207 72 696.4 0 72 208 80 696.4 0 80 209 74 696.4 0 74 210 87 696.4 0 87 211 131 696.4 0 131 212 120 696.4 0 120 213 122 696.4 0 122 214 70 696.4 0 70 215 85 696.4 0 85 216 57 696.4 0 57 217 116 696.4 0 116 218 145 696.4 0 145 219 172 696.4 0 172 220 370 696.4 0 370 221 183 696.4 0 183 222 201 696.4 0 201 223 79 696.4 0 79 224 62 696.4 0 62 225 61 696.4 0 61 226 63 696.4 0 63 227 64 696.4 0 64 228 64 696.4 0 64 229 62 696.4 0 62 230 41 696.4 0 41 231 85 696.4 0 85 232 65 696.4 0 65 233 97 696.4 0 97 234 71 696.4 0 71 235 53 696.4 0 53 236 81 696.4 0 81 237 72 696.4 0 72 238 126 696.4 0 126 239 127 696.4 0 127 240 168 696.4 0 168 241 161 696.4 0 161 242 175 696.4 0 175 243 63 696.4 0 63 244 97 696.4 0 97 245 114 696.4 0 114 246 138 696.4 0 138 247 139 696.4 0 139 248 150 696.4 0 150 249 170 696.4 0 170 250 103 696.4 0 103 251 115 696.4 0 115 252 69 696.4 0 69 253 76 696.4 0 76 254 225 696.4 0 225 255 179 696.4 0 179 256 136 696.4 0 136 257 96 696.4 0 96 258 68 696.4 0 68 259 71 696.4 0 71 260 82 696.4 0 82 261 93 696.4 0 93 262 238 696.4 0 238 263 248 696.4 0 248 264 263 696.4 0 263 265 80 696.4 0 80 266 103 696.4 0 103 267 700 696.4 0 700 268 756 696.4 0 756 269 748 696.4 0 748 270 331 696.4 0 331 271 41 696.4 0 41 272 97 696.4 0 97 273 155 696.4 0 155 274 284 696.4 0 284 275 243 696.4 0 243 276 151 696.4 0 151 277 118 696.4 0 118 278 61 696.4 0 61 279 69 696.4 0 69 280 82 696.4 0 82 281 141 696.4 0 141 282 107 696.4 0 107 283 108 696.4 0 108 284 51 696.4 0 51 285 36 696.4 0 36 286 58 696.4 0 58 287 78 696.4 0 78 288 116 696.4 0 116 289 104 696.4 0 104 290 95 696.4 0 95 291 73 696.4 0 73 292 51 696.4 0 51 293 83 696.4 0 83 294 68 696.4 0 68 295 95 696.4 0 95 296 70 696.4 0 70 297 65 696.4 0 65 298 41 696.4 0 41 299 61 696.4 0 61 300 82 696.4 0 82 301 50 696.4 0 50 Finished in 9.33 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 186,853 Reads with adapters: 82,528 (44.2%) Reads that were too short: 110 (0.1%) Reads written (passing filters): 82,418 (44.1%) Total basepairs processed: 52,986,631 bp Total written (filtered): 22,460,274 bp (42.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 82528 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 42 0.0 3 0 0 33 5 4 19 1030 0.0 3 0 836 102 61 31 20 77928 0.0 4 73686 3510 568 132 32 21 222 0.0 4 27 180 9 3 3 22 2490 0.0 4 2286 146 46 7 5 23 245 0.0 4 5 11 4 162 63 24 9 0.0 4 2 0 2 0 5 25 23 0.0 4 0 0 0 16 7 27 3 0.0 4 1 0 0 1 1 28 1 0.0 4 1 31 2 0.0 4 1 0 0 0 1 32 2 0.0 4 0 0 0 0 2 35 2 0.0 4 1 0 0 0 1 37 1 0.0 4 1 41 1 0.0 4 0 1 46 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 1 114 1 0.0 4 1 115 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 397 0.0 4 0 0 0 0 397 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 4 0.0 4 0 0 0 0 4 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 4 0.0 4 0 0 0 0 4 228 21 0.0 4 0 0 0 1 20 229 9 0.0 4 0 0 0 0 9 230 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 1 2 235 2 0.0 4 0 0 0 0 2 237 6 0.0 4 0 0 0 0 6 238 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 245 2 0.0 4 0 0 0 0 2 248 1 0.0 4 0 0 0 0 1 256 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 4 0.0 4 0 0 0 0 4 264 4 0.0 4 0 0 0 0 4 265 4 0.0 4 0 0 0 0 4 275 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 3 0.0 4 0 0 0 0 3 293 3 0.0 4 0 0 0 0 3 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.95 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 82,418 Reads with adapters: 13,635 (16.5%) Reads written (passing filters): 82,418 (100.0%) Total basepairs processed: 22,460,274 bp Total written (filtered): 22,297,325 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13635 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 10.2% G: 84.0% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 149 1287.8 0 149 4 23 321.9 0 23 6 1 20.1 0 1 7 1 5.0 0 1 9 247 0.3 0 19 228 10 3582 0.1 1 328 3254 11 9283 0.0 1 472 8811 12 217 0.0 1 7 210 13 15 0.0 1 1 14 14 9 0.0 1 0 9 15 6 0.0 1 0 6 60 1 0.0 1 0 1 73 2 0.0 1 1 1 105 1 0.0 1 0 1 107 1 0.0 1 1 111 1 0.0 1 1 139 1 0.0 1 1 151 37 0.0 1 29 8 152 2 0.0 1 2 153 1 0.0 1 1 190 3 0.0 1 0 3 209 24 0.0 1 15 9 210 1 0.0 1 1 224 6 0.0 1 5 1 225 1 0.0 1 1 226 1 0.0 1 0 1 247 1 0.0 1 0 1 258 1 0.0 1 1 259 13 0.0 1 12 1 260 1 0.0 1 1 263 1 0.0 1 1 264 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.