This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.15 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 200,098 (7.0%) Reads that were too short: 9,597 (0.3%) Reads written (passing filters): 190,501 (6.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 54,373,671 bp (6.3%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 200098 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4533 696.4 0 4533 7 85209 696.4 0 85209 8 87567 696.4 0 87567 9 113 696.4 0 113 10 29 696.4 0 29 11 22 696.4 0 22 12 31 696.4 0 31 13 26 696.4 0 26 14 26 696.4 0 26 15 35 696.4 0 35 16 34 696.4 0 34 17 22 696.4 0 22 18 42 696.4 0 42 19 34 696.4 0 34 20 29 696.4 0 29 21 38 696.4 0 38 22 26 696.4 0 26 23 23 696.4 0 23 24 20 696.4 0 20 25 16 696.4 0 16 26 16 696.4 0 16 27 24 696.4 0 24 28 17 696.4 0 17 29 22 696.4 0 22 30 22 696.4 0 22 31 22 696.4 0 22 32 20 696.4 0 20 33 45 696.4 0 45 34 30 696.4 0 30 35 26 696.4 0 26 36 27 696.4 0 27 37 36 696.4 0 36 38 22 696.4 0 22 39 31 696.4 0 31 40 63 696.4 0 63 41 44 696.4 0 44 42 21 696.4 0 21 43 25 696.4 0 25 44 33 696.4 0 33 45 45 696.4 0 45 46 52 696.4 0 52 47 34 696.4 0 34 48 33 696.4 0 33 49 26 696.4 0 26 50 27 696.4 0 27 51 31 696.4 0 31 52 51 696.4 0 51 53 28 696.4 0 28 54 82 696.4 0 82 55 410 696.4 0 410 56 65 696.4 0 65 57 22 696.4 0 22 58 18 696.4 0 18 59 46 696.4 0 46 60 35 696.4 0 35 61 158 696.4 0 158 62 164 696.4 0 164 63 198 696.4 0 198 64 32 696.4 0 32 65 56 696.4 0 56 66 36 696.4 0 36 67 85 696.4 0 85 68 58 696.4 0 58 69 87 696.4 0 87 70 54 696.4 0 54 71 22 696.4 0 22 72 25 696.4 0 25 73 26 696.4 0 26 74 34 696.4 0 34 75 29 696.4 0 29 76 58 696.4 0 58 77 40 696.4 0 40 78 63 696.4 0 63 79 46 696.4 0 46 80 68 696.4 0 68 81 38 696.4 0 38 82 28 696.4 0 28 83 49 696.4 0 49 84 38 696.4 0 38 85 38 696.4 0 38 86 36 696.4 0 36 87 63 696.4 0 63 88 59 696.4 0 59 89 51 696.4 0 51 90 51 696.4 0 51 91 56 696.4 0 56 92 40 696.4 0 40 93 25 696.4 0 25 94 37 696.4 0 37 95 50 696.4 0 50 96 60 696.4 0 60 97 64 696.4 0 64 98 81 696.4 0 81 99 43 696.4 0 43 100 31 696.4 0 31 101 43 696.4 0 43 102 30 696.4 0 30 103 27 696.4 0 27 104 38 696.4 0 38 105 43 696.4 0 43 106 20 696.4 0 20 107 38 696.4 0 38 108 38 696.4 0 38 109 29 696.4 0 29 110 28 696.4 0 28 111 36 696.4 0 36 112 54 696.4 0 54 113 49 696.4 0 49 114 38 696.4 0 38 115 56 696.4 0 56 116 36 696.4 0 36 117 30 696.4 0 30 118 31 696.4 0 31 119 41 696.4 0 41 120 42 696.4 0 42 121 54 696.4 0 54 122 53 696.4 0 53 123 123 696.4 0 123 124 78 696.4 0 78 125 114 696.4 0 114 126 255 696.4 0 255 127 168 696.4 0 168 128 429 696.4 0 429 129 331 696.4 0 331 130 351 696.4 0 351 131 173 696.4 0 173 132 110 696.4 0 110 133 43 696.4 0 43 134 88 696.4 0 88 135 77 696.4 0 77 136 97 696.4 0 97 137 42 696.4 0 42 138 51 696.4 0 51 139 42 696.4 0 42 140 404 696.4 0 404 141 47 696.4 0 47 142 29 696.4 0 29 143 93 696.4 0 93 144 35 696.4 0 35 145 63 696.4 0 63 146 39 696.4 0 39 147 48 696.4 0 48 148 49 696.4 0 49 149 59 696.4 0 59 150 44 696.4 0 44 151 57 696.4 0 57 152 36 696.4 0 36 153 20 696.4 0 20 154 37 696.4 0 37 155 44 696.4 0 44 156 41 696.4 0 41 157 24 696.4 0 24 158 33 696.4 0 33 159 45 696.4 0 45 160 40 696.4 0 40 161 30 696.4 0 30 162 35 696.4 0 35 163 39 696.4 0 39 164 51 696.4 0 51 165 56 696.4 0 56 166 163 696.4 0 163 167 135 696.4 0 135 168 118 696.4 0 118 169 35 696.4 0 35 170 33 696.4 0 33 171 61 696.4 0 61 172 47 696.4 0 47 173 39 696.4 0 39 174 68 696.4 0 68 175 55 696.4 0 55 176 58 696.4 0 58 177 45 696.4 0 45 178 37 696.4 0 37 179 40 696.4 0 40 180 43 696.4 0 43 181 31 696.4 0 31 182 42 696.4 0 42 183 77 696.4 0 77 184 77 696.4 0 77 185 69 696.4 0 69 186 49 696.4 0 49 187 55 696.4 0 55 188 40 696.4 0 40 189 52 696.4 0 52 190 36 696.4 0 36 191 46 696.4 0 46 192 77 696.4 0 77 193 61 696.4 0 61 194 71 696.4 0 71 195 80 696.4 0 80 196 237 696.4 0 237 197 547 696.4 0 547 198 532 696.4 0 532 199 574 696.4 0 574 200 87 696.4 0 87 201 57 696.4 0 57 202 62 696.4 0 62 203 43 696.4 0 43 204 58 696.4 0 58 205 48 696.4 0 48 206 49 696.4 0 49 207 51 696.4 0 51 208 62 696.4 0 62 209 47 696.4 0 47 210 52 696.4 0 52 211 91 696.4 0 91 212 70 696.4 0 70 213 79 696.4 0 79 214 60 696.4 0 60 215 70 696.4 0 70 216 54 696.4 0 54 217 141 696.4 0 141 218 123 696.4 0 123 219 146 696.4 0 146 220 279 696.4 0 279 221 153 696.4 0 153 222 184 696.4 0 184 223 79 696.4 0 79 224 63 696.4 0 63 225 62 696.4 0 62 226 66 696.4 0 66 227 54 696.4 0 54 228 61 696.4 0 61 229 46 696.4 0 46 230 46 696.4 0 46 231 45 696.4 0 45 232 52 696.4 0 52 233 68 696.4 0 68 234 55 696.4 0 55 235 59 696.4 0 59 236 74 696.4 0 74 237 52 696.4 0 52 238 147 696.4 0 147 239 135 696.4 0 135 240 124 696.4 0 124 241 108 696.4 0 108 242 141 696.4 0 141 243 65 696.4 0 65 244 74 696.4 0 74 245 104 696.4 0 104 246 136 696.4 0 136 247 115 696.4 0 115 248 104 696.4 0 104 249 167 696.4 0 167 250 70 696.4 0 70 251 105 696.4 0 105 252 54 696.4 0 54 253 59 696.4 0 59 254 320 696.4 0 320 255 257 696.4 0 257 256 168 696.4 0 168 257 72 696.4 0 72 258 71 696.4 0 71 259 57 696.4 0 57 260 47 696.4 0 47 261 52 696.4 0 52 262 303 696.4 0 303 263 322 696.4 0 322 264 443 696.4 0 443 265 54 696.4 0 54 266 56 696.4 0 56 267 252 696.4 0 252 268 292 696.4 0 292 269 287 696.4 0 287 270 158 696.4 0 158 271 33 696.4 0 33 272 54 696.4 0 54 273 108 696.4 0 108 274 136 696.4 0 136 275 130 696.4 0 130 276 83 696.4 0 83 277 60 696.4 0 60 278 39 696.4 0 39 279 44 696.4 0 44 280 55 696.4 0 55 281 84 696.4 0 84 282 83 696.4 0 83 283 59 696.4 0 59 284 41 696.4 0 41 285 29 696.4 0 29 286 39 696.4 0 39 287 40 696.4 0 40 288 85 696.4 0 85 289 64 696.4 0 64 290 49 696.4 0 49 291 52 696.4 0 52 292 39 696.4 0 39 293 60 696.4 0 60 294 54 696.4 0 54 295 68 696.4 0 68 296 45 696.4 0 45 297 46 696.4 0 46 298 38 696.4 0 38 299 46 696.4 0 46 300 51 696.4 0 51 301 60 696.4 0 60 Finished in 8.78 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 190,501 Reads with adapters: 82,141 (43.1%) Reads that were too short: 80 (0.0%) Reads written (passing filters): 82,061 (43.1%) Total basepairs processed: 54,373,671 bp Total written (filtered): 22,724,045 bp (41.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 82141 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 14 0.0 3 0 0 14 16 521 0.0 3 0 503 15 3 17 81238 0.0 3 79621 1543 68 6 18 132 0.0 3 7 124 1 19 28 0.0 3 2 0 25 1 20 79 0.0 3 0 0 0 79 21 1 0.0 3 0 0 1 24 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 1 27 1 0.0 3 0 0 0 1 42 5 0.0 3 0 0 0 5 45 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 8 0.0 3 0 0 0 8 65 4 0.0 3 0 0 0 4 66 5 0.0 3 0 0 0 5 68 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 164 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 1 192 5 0.0 3 0 0 0 5 193 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 47 0.0 3 0 0 0 47 218 3 0.0 3 0 0 1 2 224 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.85 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 82,061 Reads with adapters: 226 (0.3%) Reads written (passing filters): 82,061 (100.0%) Total basepairs processed: 22,724,045 bp Total written (filtered): 22,723,316 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 226 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.1% C: 26.5% G: 17.7% T: 6.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 191 1282.2 0 191 4 22 320.6 0 22 5 10 80.1 0 10 6 3 20.0 0 3