This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.32 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 136,593 (4.8%) Reads that were too short: 28,133 (1.0%) Reads written (passing filters): 108,460 (3.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 28,656,600 bp (3.3%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 136593 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1361 696.4 0 1361 7 37570 696.4 0 37570 8 965 696.4 0 965 9 43401 696.4 0 43401 10 58 696.4 0 58 11 25 696.4 0 25 12 17 696.4 0 17 13 7 696.4 0 7 14 7 696.4 0 7 15 17 696.4 0 17 16 17 696.4 0 17 17 8 696.4 0 8 18 11 696.4 0 11 19 3 696.4 0 3 20 10 696.4 0 10 21 29 696.4 0 29 22 10 696.4 0 10 23 31 696.4 0 31 24 8 696.4 0 8 25 6 696.4 0 6 26 7 696.4 0 7 27 4 696.4 0 4 28 4 696.4 0 4 29 13 696.4 0 13 30 18 696.4 0 18 31 12 696.4 0 12 32 12 696.4 0 12 33 13 696.4 0 13 34 7 696.4 0 7 35 12 696.4 0 12 36 25 696.4 0 25 37 9 696.4 0 9 38 10 696.4 0 10 39 19 696.4 0 19 40 22 696.4 0 22 41 132 696.4 0 132 42 48 696.4 0 48 43 34 696.4 0 34 44 12 696.4 0 12 45 12 696.4 0 12 46 12 696.4 0 12 47 13 696.4 0 13 48 12 696.4 0 12 49 10 696.4 0 10 50 12 696.4 0 12 51 29 696.4 0 29 52 21 696.4 0 21 53 27 696.4 0 27 54 17 696.4 0 17 55 18 696.4 0 18 56 19 696.4 0 19 57 28 696.4 0 28 58 16 696.4 0 16 59 10 696.4 0 10 60 16 696.4 0 16 61 13 696.4 0 13 62 16 696.4 0 16 63 17 696.4 0 17 64 10 696.4 0 10 65 19 696.4 0 19 66 21 696.4 0 21 67 30 696.4 0 30 68 27 696.4 0 27 69 139 696.4 0 139 70 222 696.4 0 222 71 137 696.4 0 137 72 16 696.4 0 16 73 23 696.4 0 23 74 29 696.4 0 29 75 46 696.4 0 46 76 47 696.4 0 47 77 32 696.4 0 32 78 59 696.4 0 59 79 22 696.4 0 22 80 40 696.4 0 40 81 33 696.4 0 33 82 30 696.4 0 30 83 26 696.4 0 26 84 40 696.4 0 40 85 69 696.4 0 69 86 66 696.4 0 66 87 211 696.4 0 211 88 123 696.4 0 123 89 131 696.4 0 131 90 182 696.4 0 182 91 94 696.4 0 94 92 71 696.4 0 71 93 85 696.4 0 85 94 117 696.4 0 117 95 82 696.4 0 82 96 65 696.4 0 65 97 97 696.4 0 97 98 102 696.4 0 102 99 94 696.4 0 94 100 1127 696.4 0 1127 101 1226 696.4 0 1226 102 3839 696.4 0 3839 103 331 696.4 0 331 104 62 696.4 0 62 105 33 696.4 0 33 106 26 696.4 0 26 107 38 696.4 0 38 108 17 696.4 0 17 109 29 696.4 0 29 110 27 696.4 0 27 111 37 696.4 0 37 112 54 696.4 0 54 113 45 696.4 0 45 114 30 696.4 0 30 115 60 696.4 0 60 116 52 696.4 0 52 117 69 696.4 0 69 118 102 696.4 0 102 119 60 696.4 0 60 120 47 696.4 0 47 121 38 696.4 0 38 122 124 696.4 0 124 123 142 696.4 0 142 124 165 696.4 0 165 125 76 696.4 0 76 126 70 696.4 0 70 127 30 696.4 0 30 128 46 696.4 0 46 129 49 696.4 0 49 130 68 696.4 0 68 131 204 696.4 0 204 132 309 696.4 0 309 133 232 696.4 0 232 134 114 696.4 0 114 135 59 696.4 0 59 136 83 696.4 0 83 137 219 696.4 0 219 138 184 696.4 0 184 139 147 696.4 0 147 140 150 696.4 0 150 141 125 696.4 0 125 142 188 696.4 0 188 143 915 696.4 0 915 144 288 696.4 0 288 145 378 696.4 0 378 146 100 696.4 0 100 147 80 696.4 0 80 148 107 696.4 0 107 149 78 696.4 0 78 150 89 696.4 0 89 151 94 696.4 0 94 152 121 696.4 0 121 153 101 696.4 0 101 154 96 696.4 0 96 155 96 696.4 0 96 156 119 696.4 0 119 157 297 696.4 0 297 158 407 696.4 0 407 159 356 696.4 0 356 160 511 696.4 0 511 161 357 696.4 0 357 162 328 696.4 0 328 163 96 696.4 0 96 164 124 696.4 0 124 165 70 696.4 0 70 166 54 696.4 0 54 167 91 696.4 0 91 168 126 696.4 0 126 169 204 696.4 0 204 170 147 696.4 0 147 171 120 696.4 0 120 172 107 696.4 0 107 173 99 696.4 0 99 174 64 696.4 0 64 175 80 696.4 0 80 176 476 696.4 0 476 177 490 696.4 0 490 178 443 696.4 0 443 179 159 696.4 0 159 180 116 696.4 0 116 181 96 696.4 0 96 182 156 696.4 0 156 183 185 696.4 0 185 184 223 696.4 0 223 185 185 696.4 0 185 186 115 696.4 0 115 187 170 696.4 0 170 188 188 696.4 0 188 189 280 696.4 0 280 190 178 696.4 0 178 191 108 696.4 0 108 192 93 696.4 0 93 193 103 696.4 0 103 194 97 696.4 0 97 195 123 696.4 0 123 196 162 696.4 0 162 197 251 696.4 0 251 198 336 696.4 0 336 199 156 696.4 0 156 200 110 696.4 0 110 201 99 696.4 0 99 202 171 696.4 0 171 203 216 696.4 0 216 204 203 696.4 0 203 205 158 696.4 0 158 206 115 696.4 0 115 207 109 696.4 0 109 208 100 696.4 0 100 209 119 696.4 0 119 210 202 696.4 0 202 211 350 696.4 0 350 212 275 696.4 0 275 213 213 696.4 0 213 214 125 696.4 0 125 215 144 696.4 0 144 216 193 696.4 0 193 217 181 696.4 0 181 218 167 696.4 0 167 219 136 696.4 0 136 220 133 696.4 0 133 221 126 696.4 0 126 222 325 696.4 0 325 223 475 696.4 0 475 224 736 696.4 0 736 225 395 696.4 0 395 226 230 696.4 0 230 227 161 696.4 0 161 228 148 696.4 0 148 229 143 696.4 0 143 230 112 696.4 0 112 231 83 696.4 0 83 232 117 696.4 0 117 233 139 696.4 0 139 234 141 696.4 0 141 235 152 696.4 0 152 236 146 696.4 0 146 237 187 696.4 0 187 238 372 696.4 0 372 239 413 696.4 0 413 240 214 696.4 0 214 241 161 696.4 0 161 242 260 696.4 0 260 243 372 696.4 0 372 244 423 696.4 0 423 245 311 696.4 0 311 246 294 696.4 0 294 247 222 696.4 0 222 248 201 696.4 0 201 249 187 696.4 0 187 250 145 696.4 0 145 251 196 696.4 0 196 252 213 696.4 0 213 253 202 696.4 0 202 254 180 696.4 0 180 255 247 696.4 0 247 256 382 696.4 0 382 257 300 696.4 0 300 258 372 696.4 0 372 259 244 696.4 0 244 260 277 696.4 0 277 261 208 696.4 0 208 262 717 696.4 0 717 263 887 696.4 0 887 264 1494 696.4 0 1494 265 657 696.4 0 657 266 484 696.4 0 484 267 185 696.4 0 185 268 125 696.4 0 125 269 160 696.4 0 160 270 227 696.4 0 227 271 423 696.4 0 423 272 408 696.4 0 408 273 397 696.4 0 397 274 429 696.4 0 429 275 259 696.4 0 259 276 343 696.4 0 343 277 228 696.4 0 228 278 300 696.4 0 300 279 250 696.4 0 250 280 198 696.4 0 198 281 191 696.4 0 191 282 241 696.4 0 241 283 309 696.4 0 309 284 382 696.4 0 382 285 318 696.4 0 318 286 309 696.4 0 309 287 292 696.4 0 292 288 245 696.4 0 245 289 203 696.4 0 203 290 240 696.4 0 240 291 378 696.4 0 378 292 507 696.4 0 507 293 340 696.4 0 340 294 303 696.4 0 303 295 171 696.4 0 171 296 153 696.4 0 153 297 186 696.4 0 186 298 267 696.4 0 267 299 315 696.4 0 315 300 382 696.4 0 382 301 708 696.4 0 708 Finished in 5.55 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 108,460 Reads with adapters: 38,774 (35.7%) Reads that were too short: 76 (0.1%) Reads written (passing filters): 38,698 (35.7%) Total basepairs processed: 28,656,600 bp Total written (filtered): 10,549,342 bp (36.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38774 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 18 0.0 3 0 0 9 5 4 19 358 0.0 3 0 280 42 28 8 20 37419 0.0 4 35598 1466 263 69 23 21 89 0.0 4 12 65 7 2 3 22 11 0.0 4 0 2 4 2 3 23 304 0.0 4 222 15 3 50 14 24 4 0.0 4 0 3 0 0 1 26 3 0.0 4 0 1 0 1 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 34 4 0.0 4 0 0 0 0 4 37 1 0.0 4 0 0 0 0 1 38 4 0.0 4 0 0 0 0 4 42 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 1 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 3 0.0 4 0 0 0 0 3 122 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 25 0.0 4 0 0 0 0 25 127 441 0.0 4 0 0 0 1 440 130 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 134 7 0.0 4 0 0 0 1 6 135 1 0.0 4 0 0 0 0 1 136 3 0.0 4 0 0 0 0 3 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 11 0.0 4 0 0 0 0 11 171 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 180 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 193 2 0.0 4 0 0 0 0 2 208 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 4 0.0 4 0 0 0 1 3 233 3 0.0 4 0 0 0 0 3 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 249 1 0.0 4 0 0 0 0 1 256 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 1 265 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.35 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 38,698 Reads with adapters: 2,265 (5.9%) Reads written (passing filters): 38,698 (100.0%) Total basepairs processed: 10,549,342 bp Total written (filtered): 10,499,210 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2265 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 22.9% C: 12.2% G: 60.4% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 173 604.7 0 173 4 14 151.2 0 14 5 2 37.8 0 2 6 1 9.4 0 1 9 46 0.1 0 5 41 10 55 0.0 1 0 55 11 1122 0.0 1 72 1050 12 369 0.0 1 8 361 13 18 0.0 1 0 18 14 1 0.0 1 0 1 15 7 0.0 1 1 6 19 2 0.0 1 1 1 20 280 0.0 1 171 109 21 24 0.0 1 12 12 22 1 0.0 1 0 1 24 1 0.0 1 1 25 32 0.0 1 20 12 28 1 0.0 1 0 1 125 1 0.0 1 0 1 131 1 0.0 1 1 134 1 0.0 1 1 149 1 0.0 1 1 152 1 0.0 1 0 1 158 4 0.0 1 4 191 2 0.0 1 0 2 193 1 0.0 1 0 1 208 1 0.0 1 1 210 55 0.0 1 38 17 211 1 0.0 1 0 1 222 1 0.0 1 0 1 224 1 0.0 1 0 1 225 45 0.0 1 43 2