This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.49 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 169,953 (6.0%) Reads that were too short: 31,017 (1.1%) Reads written (passing filters): 138,936 (4.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 39,286,746 bp (4.6%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 169953 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2865 696.4 0 2865 7 1980 696.4 0 1980 8 65611 696.4 0 65611 9 55603 696.4 0 55603 10 107 696.4 0 107 11 43 696.4 0 43 12 109 696.4 0 109 13 71 696.4 0 71 14 41 696.4 0 41 15 37 696.4 0 37 16 33 696.4 0 33 17 60 696.4 0 60 18 34 696.4 0 34 19 63 696.4 0 63 20 44 696.4 0 44 21 35 696.4 0 35 22 37 696.4 0 37 23 68 696.4 0 68 24 41 696.4 0 41 25 32 696.4 0 32 26 37 696.4 0 37 27 27 696.4 0 27 28 33 696.4 0 33 29 37 696.4 0 37 30 26 696.4 0 26 31 42 696.4 0 42 32 39 696.4 0 39 33 36 696.4 0 36 34 45 696.4 0 45 35 39 696.4 0 39 36 35 696.4 0 35 37 43 696.4 0 43 38 36 696.4 0 36 39 48 696.4 0 48 40 71 696.4 0 71 41 52 696.4 0 52 42 47 696.4 0 47 43 35 696.4 0 35 44 42 696.4 0 42 45 39 696.4 0 39 46 44 696.4 0 44 47 54 696.4 0 54 48 40 696.4 0 40 49 32 696.4 0 32 50 34 696.4 0 34 51 78 696.4 0 78 52 87 696.4 0 87 53 178 696.4 0 178 54 57 696.4 0 57 55 36 696.4 0 36 56 27 696.4 0 27 57 24 696.4 0 24 58 46 696.4 0 46 59 39 696.4 0 39 60 30 696.4 0 30 61 45 696.4 0 45 62 48 696.4 0 48 63 34 696.4 0 34 64 71 696.4 0 71 65 36 696.4 0 36 66 43 696.4 0 43 67 48 696.4 0 48 68 60 696.4 0 60 69 37 696.4 0 37 70 34 696.4 0 34 71 45 696.4 0 45 72 44 696.4 0 44 73 30 696.4 0 30 74 50 696.4 0 50 75 57 696.4 0 57 76 70 696.4 0 70 77 57 696.4 0 57 78 45 696.4 0 45 79 62 696.4 0 62 80 50 696.4 0 50 81 57 696.4 0 57 82 61 696.4 0 61 83 61 696.4 0 61 84 41 696.4 0 41 85 67 696.4 0 67 86 65 696.4 0 65 87 82 696.4 0 82 88 41 696.4 0 41 89 60 696.4 0 60 90 60 696.4 0 60 91 60 696.4 0 60 92 63 696.4 0 63 93 126 696.4 0 126 94 70 696.4 0 70 95 44 696.4 0 44 96 56 696.4 0 56 97 65 696.4 0 65 98 39 696.4 0 39 99 94 696.4 0 94 100 120 696.4 0 120 101 115 696.4 0 115 102 49 696.4 0 49 103 50 696.4 0 50 104 36 696.4 0 36 105 46 696.4 0 46 106 41 696.4 0 41 107 50 696.4 0 50 108 33 696.4 0 33 109 56 696.4 0 56 110 41 696.4 0 41 111 48 696.4 0 48 112 51 696.4 0 51 113 52 696.4 0 52 114 47 696.4 0 47 115 41 696.4 0 41 116 72 696.4 0 72 117 90 696.4 0 90 118 98 696.4 0 98 119 49 696.4 0 49 120 57 696.4 0 57 121 65 696.4 0 65 122 52 696.4 0 52 123 43 696.4 0 43 124 43 696.4 0 43 125 57 696.4 0 57 126 62 696.4 0 62 127 53 696.4 0 53 128 48 696.4 0 48 129 47 696.4 0 47 130 52 696.4 0 52 131 75 696.4 0 75 132 94 696.4 0 94 133 81 696.4 0 81 134 43 696.4 0 43 135 67 696.4 0 67 136 81 696.4 0 81 137 677 696.4 0 677 138 718 696.4 0 718 139 695 696.4 0 695 140 318 696.4 0 318 141 41 696.4 0 41 142 73 696.4 0 73 143 65 696.4 0 65 144 97 696.4 0 97 145 73 696.4 0 73 146 60 696.4 0 60 147 50 696.4 0 50 148 53 696.4 0 53 149 49 696.4 0 49 150 45 696.4 0 45 151 51 696.4 0 51 152 56 696.4 0 56 153 68 696.4 0 68 154 59 696.4 0 59 155 48 696.4 0 48 156 50 696.4 0 50 157 67 696.4 0 67 158 63 696.4 0 63 159 57 696.4 0 57 160 54 696.4 0 54 161 46 696.4 0 46 162 46 696.4 0 46 163 43 696.4 0 43 164 52 696.4 0 52 165 66 696.4 0 66 166 52 696.4 0 52 167 40 696.4 0 40 168 49 696.4 0 49 169 71 696.4 0 71 170 56 696.4 0 56 171 64 696.4 0 64 172 59 696.4 0 59 173 62 696.4 0 62 174 54 696.4 0 54 175 33 696.4 0 33 176 60 696.4 0 60 177 43 696.4 0 43 178 53 696.4 0 53 179 103 696.4 0 103 180 95 696.4 0 95 181 51 696.4 0 51 182 61 696.4 0 61 183 62 696.4 0 62 184 60 696.4 0 60 185 60 696.4 0 60 186 68 696.4 0 68 187 98 696.4 0 98 188 84 696.4 0 84 189 103 696.4 0 103 190 48 696.4 0 48 191 59 696.4 0 59 192 47 696.4 0 47 193 45 696.4 0 45 194 56 696.4 0 56 195 39 696.4 0 39 196 52 696.4 0 52 197 55 696.4 0 55 198 74 696.4 0 74 199 69 696.4 0 69 200 41 696.4 0 41 201 58 696.4 0 58 202 64 696.4 0 64 203 75 696.4 0 75 204 76 696.4 0 76 205 61 696.4 0 61 206 79 696.4 0 79 207 62 696.4 0 62 208 74 696.4 0 74 209 83 696.4 0 83 210 109 696.4 0 109 211 79 696.4 0 79 212 67 696.4 0 67 213 57 696.4 0 57 214 231 696.4 0 231 215 115 696.4 0 115 216 216 696.4 0 216 217 47 696.4 0 47 218 74 696.4 0 74 219 47 696.4 0 47 220 62 696.4 0 62 221 42 696.4 0 42 222 50 696.4 0 50 223 63 696.4 0 63 224 65 696.4 0 65 225 58 696.4 0 58 226 66 696.4 0 66 227 53 696.4 0 53 228 75 696.4 0 75 229 62 696.4 0 62 230 46 696.4 0 46 231 64 696.4 0 64 232 58 696.4 0 58 233 67 696.4 0 67 234 63 696.4 0 63 235 43 696.4 0 43 236 48 696.4 0 48 237 64 696.4 0 64 238 132 696.4 0 132 239 71 696.4 0 71 240 101 696.4 0 101 241 59 696.4 0 59 242 59 696.4 0 59 243 147 696.4 0 147 244 93 696.4 0 93 245 153 696.4 0 153 246 82 696.4 0 82 247 80 696.4 0 80 248 65 696.4 0 65 249 70 696.4 0 70 250 51 696.4 0 51 251 61 696.4 0 61 252 73 696.4 0 73 253 72 696.4 0 72 254 70 696.4 0 70 255 83 696.4 0 83 256 165 696.4 0 165 257 370 696.4 0 370 258 411 696.4 0 411 259 475 696.4 0 475 260 250 696.4 0 250 261 212 696.4 0 212 262 88 696.4 0 88 263 136 696.4 0 136 264 109 696.4 0 109 265 80 696.4 0 80 266 110 696.4 0 110 267 71 696.4 0 71 268 73 696.4 0 73 269 66 696.4 0 66 270 100 696.4 0 100 271 129 696.4 0 129 272 253 696.4 0 253 273 1296 696.4 0 1296 274 980 696.4 0 980 275 1295 696.4 0 1295 276 244 696.4 0 244 277 303 696.4 0 303 278 292 696.4 0 292 279 174 696.4 0 174 280 195 696.4 0 195 281 84 696.4 0 84 282 101 696.4 0 101 283 526 696.4 0 526 284 313 696.4 0 313 285 597 696.4 0 597 286 636 696.4 0 636 287 758 696.4 0 758 288 567 696.4 0 567 289 202 696.4 0 202 290 133 696.4 0 133 291 155 696.4 0 155 292 288 696.4 0 288 293 732 696.4 0 732 294 783 696.4 0 783 295 596 696.4 0 596 296 310 696.4 0 310 297 756 696.4 0 756 298 1879 696.4 0 1879 299 4441 696.4 0 4441 300 3841 696.4 0 3841 301 1255 696.4 0 1255 Finished in 7.00 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 138,936 Reads with adapters: 60,224 (43.3%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 60,174 (43.3%) Total basepairs processed: 39,286,746 bp Total written (filtered): 16,319,393 bp (41.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 60224 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 10 0.0 3 0 0 0 4 6 18 46 0.0 3 0 0 23 11 12 19 729 0.0 3 0 612 51 44 22 20 56838 0.0 4 52766 3206 633 172 61 21 161 0.0 4 14 123 18 5 1 22 1524 0.0 4 1420 76 18 5 5 23 345 0.0 4 2 4 1 268 70 24 8 0.0 4 1 0 0 3 4 25 3 0.0 4 0 0 0 3 27 2 0.0 4 0 0 0 1 1 28 2 0.0 4 1 1 35 1 0.0 4 1 36 1 0.0 4 1 37 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 75 2 0.0 4 2 76 2 0.0 4 2 77 1 0.0 4 0 1 78 1 0.0 4 1 92 1 0.0 4 1 94 3 0.0 4 0 0 0 0 3 107 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 460 0.0 4 0 0 0 0 460 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 136 3 0.0 4 1 0 0 0 2 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 1 153 13 0.0 4 0 0 0 0 13 165 1 0.0 4 0 0 0 0 1 172 1 0.0 4 1 193 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 1 219 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 237 5 0.0 4 0 0 0 0 5 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 4 0.0 4 0 0 0 0 4 265 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.22 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 60,174 Reads with adapters: 14,728 (24.5%) Reads written (passing filters): 60,174 (100.0%) Total basepairs processed: 16,319,393 bp Total written (filtered): 16,092,841 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14728 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 8.1% G: 85.5% T: 3.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 26 940.2 0 26 4 8 235.1 0 8 5 1 58.8 0 1 6 11 14.7 0 11 7 2 3.7 0 2 8 7 0.9 0 7 9 3002 0.2 0 379 2623 10 9364 0.1 1 680 8684 11 1888 0.0 1 75 1813 12 43 0.0 1 1 42 13 37 0.0 1 0 37 18 4 0.0 1 3 1 22 7 0.0 1 4 3 23 1 0.0 1 1 74 1 0.0 1 1 80 1 0.0 1 1 138 1 0.0 1 1 150 5 0.0 1 3 2 158 1 0.0 1 0 1 169 3 0.0 1 3 200 1 0.0 1 1 208 35 0.0 1 27 8 209 1 0.0 1 1 210 2 0.0 1 2 211 6 0.0 1 3 3 223 5 0.0 1 3 2 224 3 0.0 1 3 269 1 0.0 1 1 270 246 0.0 1 232 14 271 11 0.0 1 8 3 272 2 0.0 1 2 273 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.