This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.39 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 171,906 (6.0%) Reads that were too short: 32,512 (1.1%) Reads written (passing filters): 139,394 (4.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 39,838,191 bp (4.6%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 171906 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2617 696.4 0 2617 7 1492 696.4 0 1492 8 60032 696.4 0 60032 9 66228 696.4 0 66228 10 84 696.4 0 84 11 31 696.4 0 31 12 43 696.4 0 43 13 58 696.4 0 58 14 34 696.4 0 34 15 25 696.4 0 25 16 21 696.4 0 21 17 39 696.4 0 39 18 21 696.4 0 21 19 37 696.4 0 37 20 35 696.4 0 35 21 34 696.4 0 34 22 34 696.4 0 34 23 30 696.4 0 30 24 50 696.4 0 50 25 39 696.4 0 39 26 28 696.4 0 28 27 34 696.4 0 34 28 26 696.4 0 26 29 30 696.4 0 30 30 35 696.4 0 35 31 37 696.4 0 37 32 26 696.4 0 26 33 36 696.4 0 36 34 30 696.4 0 30 35 35 696.4 0 35 36 40 696.4 0 40 37 27 696.4 0 27 38 44 696.4 0 44 39 28 696.4 0 28 40 179 696.4 0 179 41 40 696.4 0 40 42 42 696.4 0 42 43 24 696.4 0 24 44 41 696.4 0 41 45 33 696.4 0 33 46 44 696.4 0 44 47 42 696.4 0 42 48 29 696.4 0 29 49 27 696.4 0 27 50 38 696.4 0 38 51 39 696.4 0 39 52 38 696.4 0 38 53 63 696.4 0 63 54 40 696.4 0 40 55 41 696.4 0 41 56 28 696.4 0 28 57 22 696.4 0 22 58 26 696.4 0 26 59 41 696.4 0 41 60 24 696.4 0 24 61 31 696.4 0 31 62 39 696.4 0 39 63 27 696.4 0 27 64 45 696.4 0 45 65 45 696.4 0 45 66 60 696.4 0 60 67 43 696.4 0 43 68 30 696.4 0 30 69 37 696.4 0 37 70 28 696.4 0 28 71 30 696.4 0 30 72 32 696.4 0 32 73 39 696.4 0 39 74 28 696.4 0 28 75 37 696.4 0 37 76 36 696.4 0 36 77 40 696.4 0 40 78 40 696.4 0 40 79 38 696.4 0 38 80 29 696.4 0 29 81 41 696.4 0 41 82 34 696.4 0 34 83 47 696.4 0 47 84 34 696.4 0 34 85 42 696.4 0 42 86 33 696.4 0 33 87 35 696.4 0 35 88 38 696.4 0 38 89 34 696.4 0 34 90 40 696.4 0 40 91 38 696.4 0 38 92 53 696.4 0 53 93 58 696.4 0 58 94 53 696.4 0 53 95 40 696.4 0 40 96 35 696.4 0 35 97 34 696.4 0 34 98 47 696.4 0 47 99 36 696.4 0 36 100 68 696.4 0 68 101 67 696.4 0 67 102 47 696.4 0 47 103 41 696.4 0 41 104 28 696.4 0 28 105 32 696.4 0 32 106 42 696.4 0 42 107 52 696.4 0 52 108 34 696.4 0 34 109 49 696.4 0 49 110 50 696.4 0 50 111 33 696.4 0 33 112 36 696.4 0 36 113 42 696.4 0 42 114 28 696.4 0 28 115 33 696.4 0 33 116 49 696.4 0 49 117 46 696.4 0 46 118 46 696.4 0 46 119 49 696.4 0 49 120 32 696.4 0 32 121 44 696.4 0 44 122 48 696.4 0 48 123 34 696.4 0 34 124 24 696.4 0 24 125 53 696.4 0 53 126 35 696.4 0 35 127 33 696.4 0 33 128 37 696.4 0 37 129 41 696.4 0 41 130 44 696.4 0 44 131 61 696.4 0 61 132 48 696.4 0 48 133 50 696.4 0 50 134 38 696.4 0 38 135 45 696.4 0 45 136 63 696.4 0 63 137 243 696.4 0 243 138 397 696.4 0 397 139 421 696.4 0 421 140 183 696.4 0 183 141 39 696.4 0 39 142 35 696.4 0 35 143 41 696.4 0 41 144 51 696.4 0 51 145 44 696.4 0 44 146 44 696.4 0 44 147 41 696.4 0 41 148 42 696.4 0 42 149 38 696.4 0 38 150 42 696.4 0 42 151 35 696.4 0 35 152 39 696.4 0 39 153 31 696.4 0 31 154 37 696.4 0 37 155 40 696.4 0 40 156 43 696.4 0 43 157 45 696.4 0 45 158 48 696.4 0 48 159 38 696.4 0 38 160 32 696.4 0 32 161 30 696.4 0 30 162 34 696.4 0 34 163 31 696.4 0 31 164 39 696.4 0 39 165 39 696.4 0 39 166 37 696.4 0 37 167 33 696.4 0 33 168 45 696.4 0 45 169 38 696.4 0 38 170 42 696.4 0 42 171 46 696.4 0 46 172 47 696.4 0 47 173 49 696.4 0 49 174 31 696.4 0 31 175 28 696.4 0 28 176 47 696.4 0 47 177 60 696.4 0 60 178 61 696.4 0 61 179 89 696.4 0 89 180 91 696.4 0 91 181 42 696.4 0 42 182 43 696.4 0 43 183 52 696.4 0 52 184 54 696.4 0 54 185 48 696.4 0 48 186 55 696.4 0 55 187 53 696.4 0 53 188 45 696.4 0 45 189 59 696.4 0 59 190 25 696.4 0 25 191 32 696.4 0 32 192 52 696.4 0 52 193 45 696.4 0 45 194 40 696.4 0 40 195 47 696.4 0 47 196 39 696.4 0 39 197 46 696.4 0 46 198 61 696.4 0 61 199 51 696.4 0 51 200 40 696.4 0 40 201 50 696.4 0 50 202 34 696.4 0 34 203 50 696.4 0 50 204 43 696.4 0 43 205 43 696.4 0 43 206 52 696.4 0 52 207 39 696.4 0 39 208 38 696.4 0 38 209 47 696.4 0 47 210 49 696.4 0 49 211 44 696.4 0 44 212 40 696.4 0 40 213 56 696.4 0 56 214 85 696.4 0 85 215 71 696.4 0 71 216 87 696.4 0 87 217 46 696.4 0 46 218 54 696.4 0 54 219 47 696.4 0 47 220 54 696.4 0 54 221 32 696.4 0 32 222 37 696.4 0 37 223 49 696.4 0 49 224 43 696.4 0 43 225 38 696.4 0 38 226 52 696.4 0 52 227 57 696.4 0 57 228 62 696.4 0 62 229 48 696.4 0 48 230 42 696.4 0 42 231 52 696.4 0 52 232 36 696.4 0 36 233 38 696.4 0 38 234 70 696.4 0 70 235 43 696.4 0 43 236 40 696.4 0 40 237 43 696.4 0 43 238 69 696.4 0 69 239 41 696.4 0 41 240 82 696.4 0 82 241 38 696.4 0 38 242 55 696.4 0 55 243 58 696.4 0 58 244 63 696.4 0 63 245 56 696.4 0 56 246 51 696.4 0 51 247 55 696.4 0 55 248 62 696.4 0 62 249 52 696.4 0 52 250 42 696.4 0 42 251 64 696.4 0 64 252 63 696.4 0 63 253 29 696.4 0 29 254 44 696.4 0 44 255 35 696.4 0 35 256 64 696.4 0 64 257 110 696.4 0 110 258 117 696.4 0 117 259 118 696.4 0 118 260 101 696.4 0 101 261 93 696.4 0 93 262 69 696.4 0 69 263 87 696.4 0 87 264 57 696.4 0 57 265 56 696.4 0 56 266 105 696.4 0 105 267 65 696.4 0 65 268 63 696.4 0 63 269 60 696.4 0 60 270 78 696.4 0 78 271 99 696.4 0 99 272 182 696.4 0 182 273 1390 696.4 0 1390 274 944 696.4 0 944 275 1492 696.4 0 1492 276 77 696.4 0 77 277 86 696.4 0 86 278 117 696.4 0 117 279 100 696.4 0 100 280 112 696.4 0 112 281 74 696.4 0 74 282 89 696.4 0 89 283 300 696.4 0 300 284 162 696.4 0 162 285 315 696.4 0 315 286 309 696.4 0 309 287 415 696.4 0 415 288 268 696.4 0 268 289 105 696.4 0 105 290 94 696.4 0 94 291 96 696.4 0 96 292 221 696.4 0 221 293 852 696.4 0 852 294 991 696.4 0 991 295 801 696.4 0 801 296 203 696.4 0 203 297 745 696.4 0 745 298 1859 696.4 0 1859 299 5768 696.4 0 5768 300 7070 696.4 0 7070 301 3213 696.4 0 3213 Finished in 6.50 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 139,394 Reads with adapters: 59,667 (42.8%) Reads that were too short: 93 (0.1%) Reads written (passing filters): 59,574 (42.7%) Total basepairs processed: 39,838,191 bp Total written (filtered): 16,440,278 bp (41.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 59667 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 9 2 16 369 0.0 3 0 362 7 17 58907 0.0 3 57709 1154 41 3 18 142 0.0 3 9 124 9 19 27 0.0 3 5 0 19 3 20 93 0.0 3 1 0 0 92 24 1 0.0 3 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 1 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 9 0.0 3 0 0 0 9 58 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 74 1 0.0 3 1 83 1 0.0 3 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 116 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 197 1 0.0 3 0 1 201 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 47 0.0 3 0 0 1 46 218 1 0.0 3 0 0 0 1 219 17 0.0 3 0 0 1 16 222 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 280 5 0.0 3 0 0 0 5 281 3 0.0 3 0 0 0 3 282 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 59,574 Reads with adapters: 242 (0.4%) Reads written (passing filters): 59,574 (100.0%) Total basepairs processed: 16,440,278 bp Total written (filtered): 16,439,442 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 242 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.7% C: 8.7% G: 27.3% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 136 930.8 0 136 4 102 232.7 0 102 5 4 58.2 0 4