This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.27 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 152,878 (5.4%) Reads that were too short: 28,680 (1.0%) Reads written (passing filters): 124,198 (4.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 32,021,314 bp (3.7%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 152878 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3753 696.4 0 3753 7 43080 696.4 0 43080 8 43820 696.4 0 43820 9 69 696.4 0 69 10 36 696.4 0 36 11 29 696.4 0 29 12 23 696.4 0 23 13 24 696.4 0 24 14 19 696.4 0 19 15 25 696.4 0 25 16 14 696.4 0 14 17 16 696.4 0 16 18 16 696.4 0 16 19 24 696.4 0 24 20 26 696.4 0 26 21 18 696.4 0 18 22 16 696.4 0 16 23 29 696.4 0 29 24 22 696.4 0 22 25 25 696.4 0 25 26 24 696.4 0 24 27 22 696.4 0 22 28 19 696.4 0 19 29 31 696.4 0 31 30 61 696.4 0 61 31 21 696.4 0 21 32 23 696.4 0 23 33 17 696.4 0 17 34 30 696.4 0 30 35 26 696.4 0 26 36 20 696.4 0 20 37 47 696.4 0 47 38 15 696.4 0 15 39 30 696.4 0 30 40 41 696.4 0 41 41 21 696.4 0 21 42 23 696.4 0 23 43 32 696.4 0 32 44 37 696.4 0 37 45 29 696.4 0 29 46 43 696.4 0 43 47 57 696.4 0 57 48 63 696.4 0 63 49 121 696.4 0 121 50 1502 696.4 0 1502 51 870 696.4 0 870 52 1134 696.4 0 1134 53 39 696.4 0 39 54 39 696.4 0 39 55 14 696.4 0 14 56 19 696.4 0 19 57 14 696.4 0 14 58 29 696.4 0 29 59 29 696.4 0 29 60 47 696.4 0 47 61 59 696.4 0 59 62 42 696.4 0 42 63 43 696.4 0 43 64 72 696.4 0 72 65 47 696.4 0 47 66 56 696.4 0 56 67 43 696.4 0 43 68 69 696.4 0 69 69 95 696.4 0 95 70 22 696.4 0 22 71 65 696.4 0 65 72 58 696.4 0 58 73 138 696.4 0 138 74 651 696.4 0 651 75 437 696.4 0 437 76 1568 696.4 0 1568 77 105 696.4 0 105 78 212 696.4 0 212 79 53 696.4 0 53 80 91 696.4 0 91 81 26 696.4 0 26 82 37 696.4 0 37 83 38 696.4 0 38 84 44 696.4 0 44 85 40 696.4 0 40 86 38 696.4 0 38 87 28 696.4 0 28 88 33 696.4 0 33 89 44 696.4 0 44 90 32 696.4 0 32 91 84 696.4 0 84 92 67 696.4 0 67 93 44 696.4 0 44 94 55 696.4 0 55 95 30 696.4 0 30 96 19 696.4 0 19 97 38 696.4 0 38 98 39 696.4 0 39 99 23 696.4 0 23 100 20 696.4 0 20 101 33 696.4 0 33 102 22 696.4 0 22 103 16 696.4 0 16 104 26 696.4 0 26 105 20 696.4 0 20 106 24 696.4 0 24 107 34 696.4 0 34 108 21 696.4 0 21 109 21 696.4 0 21 110 20 696.4 0 20 111 26 696.4 0 26 112 55 696.4 0 55 113 56 696.4 0 56 114 62 696.4 0 62 115 135 696.4 0 135 116 81 696.4 0 81 117 125 696.4 0 125 118 76 696.4 0 76 119 80 696.4 0 80 120 72 696.4 0 72 121 53 696.4 0 53 122 57 696.4 0 57 123 61 696.4 0 61 124 85 696.4 0 85 125 1073 696.4 0 1073 126 489 696.4 0 489 127 845 696.4 0 845 128 71 696.4 0 71 129 68 696.4 0 68 130 344 696.4 0 344 131 142 696.4 0 142 132 194 696.4 0 194 133 51 696.4 0 51 134 64 696.4 0 64 135 62 696.4 0 62 136 71 696.4 0 71 137 113 696.4 0 113 138 31 696.4 0 31 139 28 696.4 0 28 140 31 696.4 0 31 141 38 696.4 0 38 142 43 696.4 0 43 143 45 696.4 0 45 144 53 696.4 0 53 145 41 696.4 0 41 146 56 696.4 0 56 147 48 696.4 0 48 148 40 696.4 0 40 149 38 696.4 0 38 150 42 696.4 0 42 151 51 696.4 0 51 152 40 696.4 0 40 153 59 696.4 0 59 154 53 696.4 0 53 155 50 696.4 0 50 156 62 696.4 0 62 157 44 696.4 0 44 158 70 696.4 0 70 159 54 696.4 0 54 160 49 696.4 0 49 161 88 696.4 0 88 162 74 696.4 0 74 163 122 696.4 0 122 164 1535 696.4 0 1535 165 957 696.4 0 957 166 2321 696.4 0 2321 167 53 696.4 0 53 168 42 696.4 0 42 169 60 696.4 0 60 170 72 696.4 0 72 171 75 696.4 0 75 172 55 696.4 0 55 173 41 696.4 0 41 174 50 696.4 0 50 175 40 696.4 0 40 176 31 696.4 0 31 177 41 696.4 0 41 178 74 696.4 0 74 179 143 696.4 0 143 180 109 696.4 0 109 181 153 696.4 0 153 182 102 696.4 0 102 183 123 696.4 0 123 184 90 696.4 0 90 185 60 696.4 0 60 186 101 696.4 0 101 187 82 696.4 0 82 188 66 696.4 0 66 189 119 696.4 0 119 190 132 696.4 0 132 191 456 696.4 0 456 192 280 696.4 0 280 193 339 696.4 0 339 194 325 696.4 0 325 195 264 696.4 0 264 196 2509 696.4 0 2509 197 2159 696.4 0 2159 198 2735 696.4 0 2735 199 1266 696.4 0 1266 200 304 696.4 0 304 201 258 696.4 0 258 202 124 696.4 0 124 203 67 696.4 0 67 204 125 696.4 0 125 205 141 696.4 0 141 206 100 696.4 0 100 207 67 696.4 0 67 208 88 696.4 0 88 209 115 696.4 0 115 210 123 696.4 0 123 211 166 696.4 0 166 212 132 696.4 0 132 213 120 696.4 0 120 214 146 696.4 0 146 215 164 696.4 0 164 216 1049 696.4 0 1049 217 698 696.4 0 698 218 806 696.4 0 806 219 285 696.4 0 285 220 176 696.4 0 176 221 130 696.4 0 130 222 186 696.4 0 186 223 137 696.4 0 137 224 84 696.4 0 84 225 90 696.4 0 90 226 78 696.4 0 78 227 85 696.4 0 85 228 77 696.4 0 77 229 105 696.4 0 105 230 95 696.4 0 95 231 75 696.4 0 75 232 71 696.4 0 71 233 66 696.4 0 66 234 103 696.4 0 103 235 83 696.4 0 83 236 59 696.4 0 59 237 90 696.4 0 90 238 121 696.4 0 121 239 189 696.4 0 189 240 156 696.4 0 156 241 167 696.4 0 167 242 116 696.4 0 116 243 99 696.4 0 99 244 99 696.4 0 99 245 144 696.4 0 144 246 192 696.4 0 192 247 895 696.4 0 895 248 1375 696.4 0 1375 249 1541 696.4 0 1541 250 1249 696.4 0 1249 251 394 696.4 0 394 252 162 696.4 0 162 253 83 696.4 0 83 254 137 696.4 0 137 255 74 696.4 0 74 256 122 696.4 0 122 257 122 696.4 0 122 258 133 696.4 0 133 259 105 696.4 0 105 260 68 696.4 0 68 261 96 696.4 0 96 262 88 696.4 0 88 263 83 696.4 0 83 264 79 696.4 0 79 265 81 696.4 0 81 266 98 696.4 0 98 267 83 696.4 0 83 268 68 696.4 0 68 269 347 696.4 0 347 270 230 696.4 0 230 271 381 696.4 0 381 272 90 696.4 0 90 273 64 696.4 0 64 274 63 696.4 0 63 275 82 696.4 0 82 276 152 696.4 0 152 277 116 696.4 0 116 278 166 696.4 0 166 279 145 696.4 0 145 280 129 696.4 0 129 281 96 696.4 0 96 282 127 696.4 0 127 283 137 696.4 0 137 284 183 696.4 0 183 285 184 696.4 0 184 286 173 696.4 0 173 287 180 696.4 0 180 288 218 696.4 0 218 289 1487 696.4 0 1487 290 2035 696.4 0 2035 291 2884 696.4 0 2884 292 1598 696.4 0 1598 293 297 696.4 0 297 294 182 696.4 0 182 295 109 696.4 0 109 296 123 696.4 0 123 297 341 696.4 0 341 298 495 696.4 0 495 299 451 696.4 0 451 300 400 696.4 0 400 301 290 696.4 0 290 Finished in 6.15 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 124,198 Reads with adapters: 45,736 (36.8%) Reads that were too short: 82 (0.1%) Reads written (passing filters): 45,654 (36.8%) Total basepairs processed: 32,021,314 bp Total written (filtered): 12,447,788 bp (38.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45736 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 2 3 18 24 0.0 3 0 0 19 4 1 19 594 0.0 3 0 478 50 41 25 20 44645 0.0 4 42215 2036 292 61 41 21 123 0.0 4 20 92 11 22 21 0.0 4 10 1 8 1 1 23 109 0.0 4 0 0 1 76 32 25 2 0.0 4 0 0 0 0 2 26 2 0.0 4 0 0 0 1 1 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 36 1 0.0 4 0 1 41 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 73 2 0.0 4 0 0 0 0 2 74 2 0.0 4 0 0 0 0 2 75 3 0.0 4 0 0 0 0 3 78 1 0.0 4 0 0 0 0 1 84 13 0.0 4 0 0 0 0 13 86 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 99 0.0 4 0 0 0 0 99 128 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 157 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 180 1 0.0 4 1 184 1 0.0 4 0 0 0 0 1 185 7 0.0 4 0 0 0 0 7 186 8 0.0 4 0 0 0 1 7 187 2 0.0 4 0 0 0 1 1 191 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 1 0 1 228 2 0.0 4 1 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 244 6 0.0 4 0 0 0 0 6 247 2 0.0 4 0 0 0 0 2 257 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 262 5 0.0 4 0 0 0 0 5 263 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.67 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 45,654 Reads with adapters: 8,794 (19.3%) Reads written (passing filters): 45,654 (100.0%) Total basepairs processed: 12,447,788 bp Total written (filtered): 12,341,223 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8794 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 9.3% G: 85.2% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 95 713.3 0 95 4 19 178.3 0 19 8 1 0.7 0 1 9 66 0.2 0 3 63 10 1347 0.0 1 133 1214 11 7093 0.0 1 343 6750 12 91 0.0 1 2 89 13 12 0.0 1 0 12 14 7 0.0 1 0 7 15 1 0.0 1 0 1 73 1 0.0 1 0 1 151 2 0.0 1 1 1 153 1 0.0 1 1 209 46 0.0 1 40 6 210 1 0.0 1 1 211 9 0.0 1 9 224 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.