This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.78 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 131,921 (4.6%) Reads that were too short: 14,934 (0.5%) Reads written (passing filters): 116,987 (4.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 31,356,400 bp (3.7%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 131921 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3975 696.4 0 3975 7 43642 696.4 0 43642 8 45454 696.4 0 45454 9 56 696.4 0 56 10 39 696.4 0 39 11 48 696.4 0 48 12 30 696.4 0 30 13 34 696.4 0 34 14 30 696.4 0 30 15 25 696.4 0 25 16 19 696.4 0 19 17 22 696.4 0 22 18 18 696.4 0 18 19 23 696.4 0 23 20 22 696.4 0 22 21 23 696.4 0 23 22 23 696.4 0 23 23 31 696.4 0 31 24 23 696.4 0 23 25 26 696.4 0 26 26 26 696.4 0 26 27 30 696.4 0 30 28 42 696.4 0 42 29 25 696.4 0 25 30 82 696.4 0 82 31 19 696.4 0 19 32 27 696.4 0 27 33 46 696.4 0 46 34 25 696.4 0 25 35 27 696.4 0 27 36 32 696.4 0 32 37 43 696.4 0 43 38 24 696.4 0 24 39 42 696.4 0 42 40 58 696.4 0 58 41 30 696.4 0 30 42 31 696.4 0 31 43 54 696.4 0 54 44 32 696.4 0 32 45 34 696.4 0 34 46 27 696.4 0 27 47 56 696.4 0 56 48 54 696.4 0 54 49 82 696.4 0 82 50 1363 696.4 0 1363 51 709 696.4 0 709 52 946 696.4 0 946 53 31 696.4 0 31 54 26 696.4 0 26 55 18 696.4 0 18 56 12 696.4 0 12 57 27 696.4 0 27 58 23 696.4 0 23 59 22 696.4 0 22 60 34 696.4 0 34 61 36 696.4 0 36 62 20 696.4 0 20 63 58 696.4 0 58 64 45 696.4 0 45 65 48 696.4 0 48 66 39 696.4 0 39 67 40 696.4 0 40 68 56 696.4 0 56 69 96 696.4 0 96 70 28 696.4 0 28 71 32 696.4 0 32 72 52 696.4 0 52 73 59 696.4 0 59 74 332 696.4 0 332 75 252 696.4 0 252 76 771 696.4 0 771 77 41 696.4 0 41 78 80 696.4 0 80 79 41 696.4 0 41 80 52 696.4 0 52 81 29 696.4 0 29 82 24 696.4 0 24 83 26 696.4 0 26 84 31 696.4 0 31 85 31 696.4 0 31 86 30 696.4 0 30 87 32 696.4 0 32 88 27 696.4 0 27 89 21 696.4 0 21 90 31 696.4 0 31 91 38 696.4 0 38 92 22 696.4 0 22 93 23 696.4 0 23 94 19 696.4 0 19 95 22 696.4 0 22 96 22 696.4 0 22 97 29 696.4 0 29 98 40 696.4 0 40 99 38 696.4 0 38 100 39 696.4 0 39 101 37 696.4 0 37 102 32 696.4 0 32 103 49 696.4 0 49 104 30 696.4 0 30 105 27 696.4 0 27 106 38 696.4 0 38 107 40 696.4 0 40 108 31 696.4 0 31 109 27 696.4 0 27 110 30 696.4 0 30 111 43 696.4 0 43 112 46 696.4 0 46 113 39 696.4 0 39 114 59 696.4 0 59 115 91 696.4 0 91 116 76 696.4 0 76 117 79 696.4 0 79 118 136 696.4 0 136 119 64 696.4 0 64 120 48 696.4 0 48 121 46 696.4 0 46 122 60 696.4 0 60 123 77 696.4 0 77 124 75 696.4 0 75 125 865 696.4 0 865 126 343 696.4 0 343 127 561 696.4 0 561 128 60 696.4 0 60 129 72 696.4 0 72 130 187 696.4 0 187 131 102 696.4 0 102 132 126 696.4 0 126 133 49 696.4 0 49 134 41 696.4 0 41 135 71 696.4 0 71 136 43 696.4 0 43 137 113 696.4 0 113 138 48 696.4 0 48 139 40 696.4 0 40 140 21 696.4 0 21 141 37 696.4 0 37 142 47 696.4 0 47 143 26 696.4 0 26 144 36 696.4 0 36 145 45 696.4 0 45 146 42 696.4 0 42 147 35 696.4 0 35 148 35 696.4 0 35 149 38 696.4 0 38 150 45 696.4 0 45 151 43 696.4 0 43 152 33 696.4 0 33 153 52 696.4 0 52 154 50 696.4 0 50 155 33 696.4 0 33 156 36 696.4 0 36 157 58 696.4 0 58 158 65 696.4 0 65 159 53 696.4 0 53 160 47 696.4 0 47 161 80 696.4 0 80 162 76 696.4 0 76 163 109 696.4 0 109 164 1467 696.4 0 1467 165 888 696.4 0 888 166 2213 696.4 0 2213 167 40 696.4 0 40 168 53 696.4 0 53 169 33 696.4 0 33 170 30 696.4 0 30 171 47 696.4 0 47 172 30 696.4 0 30 173 28 696.4 0 28 174 38 696.4 0 38 175 32 696.4 0 32 176 43 696.4 0 43 177 53 696.4 0 53 178 57 696.4 0 57 179 53 696.4 0 53 180 36 696.4 0 36 181 82 696.4 0 82 182 57 696.4 0 57 183 88 696.4 0 88 184 52 696.4 0 52 185 46 696.4 0 46 186 74 696.4 0 74 187 65 696.4 0 65 188 41 696.4 0 41 189 75 696.4 0 75 190 77 696.4 0 77 191 264 696.4 0 264 192 130 696.4 0 130 193 206 696.4 0 206 194 123 696.4 0 123 195 144 696.4 0 144 196 1334 696.4 0 1334 197 1111 696.4 0 1111 198 1346 696.4 0 1346 199 627 696.4 0 627 200 146 696.4 0 146 201 141 696.4 0 141 202 79 696.4 0 79 203 58 696.4 0 58 204 85 696.4 0 85 205 132 696.4 0 132 206 92 696.4 0 92 207 58 696.4 0 58 208 73 696.4 0 73 209 86 696.4 0 86 210 89 696.4 0 89 211 90 696.4 0 90 212 84 696.4 0 84 213 110 696.4 0 110 214 85 696.4 0 85 215 88 696.4 0 88 216 528 696.4 0 528 217 385 696.4 0 385 218 386 696.4 0 386 219 159 696.4 0 159 220 54 696.4 0 54 221 59 696.4 0 59 222 85 696.4 0 85 223 78 696.4 0 78 224 77 696.4 0 77 225 77 696.4 0 77 226 40 696.4 0 40 227 60 696.4 0 60 228 52 696.4 0 52 229 56 696.4 0 56 230 58 696.4 0 58 231 51 696.4 0 51 232 42 696.4 0 42 233 36 696.4 0 36 234 57 696.4 0 57 235 60 696.4 0 60 236 50 696.4 0 50 237 59 696.4 0 59 238 64 696.4 0 64 239 125 696.4 0 125 240 114 696.4 0 114 241 89 696.4 0 89 242 70 696.4 0 70 243 50 696.4 0 50 244 52 696.4 0 52 245 40 696.4 0 40 246 84 696.4 0 84 247 369 696.4 0 369 248 761 696.4 0 761 249 867 696.4 0 867 250 880 696.4 0 880 251 173 696.4 0 173 252 75 696.4 0 75 253 51 696.4 0 51 254 57 696.4 0 57 255 52 696.4 0 52 256 60 696.4 0 60 257 56 696.4 0 56 258 68 696.4 0 68 259 64 696.4 0 64 260 55 696.4 0 55 261 43 696.4 0 43 262 58 696.4 0 58 263 50 696.4 0 50 264 57 696.4 0 57 265 34 696.4 0 34 266 56 696.4 0 56 267 66 696.4 0 66 268 57 696.4 0 57 269 406 696.4 0 406 270 187 696.4 0 187 271 373 696.4 0 373 272 55 696.4 0 55 273 40 696.4 0 40 274 41 696.4 0 41 275 54 696.4 0 54 276 70 696.4 0 70 277 61 696.4 0 61 278 71 696.4 0 71 279 101 696.4 0 101 280 61 696.4 0 61 281 63 696.4 0 63 282 65 696.4 0 65 283 76 696.4 0 76 284 79 696.4 0 79 285 74 696.4 0 74 286 78 696.4 0 78 287 71 696.4 0 71 288 77 696.4 0 77 289 511 696.4 0 511 290 770 696.4 0 770 291 1268 696.4 0 1268 292 726 696.4 0 726 293 142 696.4 0 142 294 103 696.4 0 103 295 50 696.4 0 50 296 81 696.4 0 81 297 182 696.4 0 182 298 155 696.4 0 155 299 182 696.4 0 182 300 132 696.4 0 132 301 114 696.4 0 114 Finished in 5.40 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 116,987 Reads with adapters: 46,163 (39.5%) Reads that were too short: 92 (0.1%) Reads written (passing filters): 46,071 (39.4%) Total basepairs processed: 31,356,400 bp Total written (filtered): 12,746,893 bp (40.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46163 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 247 0.0 3 0 241 6 17 45541 0.0 3 44677 828 31 5 18 83 0.0 3 1 77 5 19 24 0.0 3 7 1 16 20 57 0.0 3 1 0 0 56 22 1 0.0 3 0 0 0 1 23 1 0.0 3 1 26 2 0.0 3 0 0 0 2 27 39 0.0 3 0 0 0 39 30 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 46 11 0.0 3 0 0 0 11 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 77 1 0.0 3 0 0 1 78 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 10 0.0 3 0 0 0 10 89 1 0.0 3 0 0 0 1 90 10 0.0 3 0 0 0 10 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 100 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 12 0.0 3 0 0 0 12 118 4 0.0 3 0 0 0 4 119 1 0.0 3 0 0 0 1 120 3 0.0 3 0 0 0 3 121 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 1 0 0 1 136 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 177 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 205 4 0.0 3 0 0 0 4 212 1 0.0 3 0 0 0 1 217 33 0.0 3 0 0 1 32 218 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.63 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 46,071 Reads with adapters: 73 (0.2%) Reads written (passing filters): 46,071 (100.0%) Total basepairs processed: 12,746,893 bp Total written (filtered): 12,746,658 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 73 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.4% C: 16.4% G: 41.1% T: 15.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 63 719.9 0 63 4 6 180.0 0 6 5 2 45.0 0 2 6 2 11.2 0 2