This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.28 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 299,080 (10.5%) Reads that were too short: 111,565 (3.9%) Reads written (passing filters): 187,515 (6.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 49,539,055 bp (5.8%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 299080 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2105 696.4 0 2105 7 71574 696.4 0 71574 8 1914 696.4 0 1914 9 69097 696.4 0 69097 10 129 696.4 0 129 11 39 696.4 0 39 12 42 696.4 0 42 13 20 696.4 0 20 14 48 696.4 0 48 15 86 696.4 0 86 16 77 696.4 0 77 17 34 696.4 0 34 18 53 696.4 0 53 19 34 696.4 0 34 20 32 696.4 0 32 21 17 696.4 0 17 22 29 696.4 0 29 23 41 696.4 0 41 24 32 696.4 0 32 25 32 696.4 0 32 26 35 696.4 0 35 27 30 696.4 0 30 28 40 696.4 0 40 29 21 696.4 0 21 30 41 696.4 0 41 31 32 696.4 0 32 32 54 696.4 0 54 33 37 696.4 0 37 34 37 696.4 0 37 35 42 696.4 0 42 36 30 696.4 0 30 37 44 696.4 0 44 38 51 696.4 0 51 39 57 696.4 0 57 40 45 696.4 0 45 41 42 696.4 0 42 42 71 696.4 0 71 43 39 696.4 0 39 44 36 696.4 0 36 45 21 696.4 0 21 46 29 696.4 0 29 47 31 696.4 0 31 48 58 696.4 0 58 49 33 696.4 0 33 50 59 696.4 0 59 51 32 696.4 0 32 52 32 696.4 0 32 53 36 696.4 0 36 54 46 696.4 0 46 55 70 696.4 0 70 56 99 696.4 0 99 57 977 696.4 0 977 58 972 696.4 0 972 59 645 696.4 0 645 60 30 696.4 0 30 61 32 696.4 0 32 62 36 696.4 0 36 63 46 696.4 0 46 64 44 696.4 0 44 65 28 696.4 0 28 66 30 696.4 0 30 67 31 696.4 0 31 68 52 696.4 0 52 69 34 696.4 0 34 70 37 696.4 0 37 71 24 696.4 0 24 72 44 696.4 0 44 73 54 696.4 0 54 74 45 696.4 0 45 75 42 696.4 0 42 76 45 696.4 0 45 77 46 696.4 0 46 78 52 696.4 0 52 79 71 696.4 0 71 80 79 696.4 0 79 81 56 696.4 0 56 82 46 696.4 0 46 83 62 696.4 0 62 84 50 696.4 0 50 85 40 696.4 0 40 86 58 696.4 0 58 87 47 696.4 0 47 88 151 696.4 0 151 89 93 696.4 0 93 90 66 696.4 0 66 91 83 696.4 0 83 92 119 696.4 0 119 93 42 696.4 0 42 94 54 696.4 0 54 95 62 696.4 0 62 96 37 696.4 0 37 97 55 696.4 0 55 98 110 696.4 0 110 99 87 696.4 0 87 100 41 696.4 0 41 101 43 696.4 0 43 102 49 696.4 0 49 103 43 696.4 0 43 104 88 696.4 0 88 105 152 696.4 0 152 106 126 696.4 0 126 107 143 696.4 0 143 108 151 696.4 0 151 109 107 696.4 0 107 110 82 696.4 0 82 111 58 696.4 0 58 112 54 696.4 0 54 113 67 696.4 0 67 114 88 696.4 0 88 115 103 696.4 0 103 116 72 696.4 0 72 117 112 696.4 0 112 118 65 696.4 0 65 119 100 696.4 0 100 120 151 696.4 0 151 121 256 696.4 0 256 122 1677 696.4 0 1677 123 746 696.4 0 746 124 774 696.4 0 774 125 114 696.4 0 114 126 93 696.4 0 93 127 156 696.4 0 156 128 3476 696.4 0 3476 129 100 696.4 0 100 130 135 696.4 0 135 131 116 696.4 0 116 132 98 696.4 0 98 133 70 696.4 0 70 134 98 696.4 0 98 135 160 696.4 0 160 136 120 696.4 0 120 137 108 696.4 0 108 138 109 696.4 0 109 139 124 696.4 0 124 140 125 696.4 0 125 141 421 696.4 0 421 142 568 696.4 0 568 143 362 696.4 0 362 144 166 696.4 0 166 145 119 696.4 0 119 146 161 696.4 0 161 147 263 696.4 0 263 148 311 696.4 0 311 149 335 696.4 0 335 150 419 696.4 0 419 151 4245 696.4 0 4245 152 5657 696.4 0 5657 153 4027 696.4 0 4027 154 132 696.4 0 132 155 165 696.4 0 165 156 131 696.4 0 131 157 358 696.4 0 358 158 278 696.4 0 278 159 293 696.4 0 293 160 118 696.4 0 118 161 236 696.4 0 236 162 430 696.4 0 430 163 264 696.4 0 264 164 145 696.4 0 145 165 103 696.4 0 103 166 103 696.4 0 103 167 189 696.4 0 189 168 163 696.4 0 163 169 105 696.4 0 105 170 173 696.4 0 173 171 100 696.4 0 100 172 83 696.4 0 83 173 54 696.4 0 54 174 70 696.4 0 70 175 113 696.4 0 113 176 237 696.4 0 237 177 158 696.4 0 158 178 107 696.4 0 107 179 94 696.4 0 94 180 78 696.4 0 78 181 102 696.4 0 102 182 394 696.4 0 394 183 337 696.4 0 337 184 240 696.4 0 240 185 105 696.4 0 105 186 211 696.4 0 211 187 209 696.4 0 209 188 176 696.4 0 176 189 158 696.4 0 158 190 80 696.4 0 80 191 77 696.4 0 77 192 72 696.4 0 72 193 93 696.4 0 93 194 135 696.4 0 135 195 242 696.4 0 242 196 397 696.4 0 397 197 264 696.4 0 264 198 184 696.4 0 184 199 101 696.4 0 101 200 107 696.4 0 107 201 168 696.4 0 168 202 167 696.4 0 167 203 140 696.4 0 140 204 150 696.4 0 150 205 165 696.4 0 165 206 240 696.4 0 240 207 279 696.4 0 279 208 546 696.4 0 546 209 1709 696.4 0 1709 210 1996 696.4 0 1996 211 1766 696.4 0 1766 212 2517 696.4 0 2517 213 22478 696.4 0 22478 214 34431 696.4 0 34431 215 16957 696.4 0 16957 216 490 696.4 0 490 217 175 696.4 0 175 218 164 696.4 0 164 219 219 696.4 0 219 220 436 696.4 0 436 221 2380 696.4 0 2380 222 3310 696.4 0 3310 223 2828 696.4 0 2828 224 224 696.4 0 224 225 95 696.4 0 95 226 108 696.4 0 108 227 110 696.4 0 110 228 93 696.4 0 93 229 85 696.4 0 85 230 68 696.4 0 68 231 173 696.4 0 173 232 172 696.4 0 172 233 162 696.4 0 162 234 121 696.4 0 121 235 118 696.4 0 118 236 126 696.4 0 126 237 101 696.4 0 101 238 93 696.4 0 93 239 104 696.4 0 104 240 64 696.4 0 64 241 89 696.4 0 89 242 159 696.4 0 159 243 223 696.4 0 223 244 392 696.4 0 392 245 377 696.4 0 377 246 170 696.4 0 170 247 90 696.4 0 90 248 98 696.4 0 98 249 103 696.4 0 103 250 127 696.4 0 127 251 117 696.4 0 117 252 106 696.4 0 106 253 114 696.4 0 114 254 139 696.4 0 139 255 187 696.4 0 187 256 256 696.4 0 256 257 683 696.4 0 683 258 814 696.4 0 814 259 710 696.4 0 710 260 376 696.4 0 376 261 411 696.4 0 411 262 439 696.4 0 439 263 1058 696.4 0 1058 264 437 696.4 0 437 265 452 696.4 0 452 266 160 696.4 0 160 267 74 696.4 0 74 268 126 696.4 0 126 269 344 696.4 0 344 270 427 696.4 0 427 271 392 696.4 0 392 272 269 696.4 0 269 273 230 696.4 0 230 274 144 696.4 0 144 275 212 696.4 0 212 276 251 696.4 0 251 277 249 696.4 0 249 278 207 696.4 0 207 279 158 696.4 0 158 280 121 696.4 0 121 281 256 696.4 0 256 282 284 696.4 0 284 283 341 696.4 0 341 284 422 696.4 0 422 285 410 696.4 0 410 286 312 696.4 0 312 287 122 696.4 0 122 288 105 696.4 0 105 289 167 696.4 0 167 290 211 696.4 0 211 291 238 696.4 0 238 292 173 696.4 0 173 293 101 696.4 0 101 294 138 696.4 0 138 295 186 696.4 0 186 296 196 696.4 0 196 297 235 696.4 0 235 298 205 696.4 0 205 299 141 696.4 0 141 300 148 696.4 0 148 301 123 696.4 0 123 Finished in 9.21 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 187,515 Reads with adapters: 72,668 (38.8%) Reads that were too short: 70 (0.0%) Reads written (passing filters): 72,598 (38.7%) Total basepairs processed: 49,539,055 bp Total written (filtered): 19,846,227 bp (40.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 72668 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 36 0.0 3 0 0 23 5 8 19 592 0.0 3 0 515 40 23 14 20 71277 0.0 4 68541 2258 371 83 24 21 170 0.0 4 18 144 6 2 22 28 0.0 4 13 1 11 1 2 23 83 0.0 4 2 1 0 68 12 24 1 0.0 4 0 0 0 0 1 25 3 0.0 4 1 0 1 1 27 60 0.0 4 0 0 0 0 60 28 5 0.0 4 1 0 0 0 4 32 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 84 2 0.0 4 2 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 1 101 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 4 0.0 4 0 0 0 0 4 127 315 0.0 4 0 0 0 0 315 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 5 0.0 4 0 0 0 0 5 133 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 153 6 0.0 4 0 0 0 0 6 190 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 3 0.0 4 0 0 0 0 3 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 228 10 0.0 4 0 0 0 0 10 229 3 0.0 4 0 0 0 1 2 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 263 3 0.0 4 0 0 0 0 3 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 284 2 0.0 4 0 0 0 1 1 291 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.54 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 72,598 Reads with adapters: 4,467 (6.2%) Reads written (passing filters): 72,598 (100.0%) Total basepairs processed: 19,846,227 bp Total written (filtered): 19,645,050 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4467 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 19.9% C: 11.2% G: 66.9% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 308 1134.3 0 308 4 35 283.6 0 35 5 2 70.9 0 2 9 20 0.3 0 2 18 10 76 0.1 1 5 71 11 2548 0.0 1 121 2427 12 778 0.0 1 11 767 13 49 0.0 1 1 48 14 3 0.0 1 0 3 15 9 0.0 1 0 9 16 1 0.0 1 0 1 20 1 0.0 1 0 1 28 1 0.0 1 1 152 7 0.0 1 4 3 171 1 0.0 1 1 191 6 0.0 1 1 5 209 1 0.0 1 1 210 67 0.0 1 55 12 211 1 0.0 1 1 225 7 0.0 1 7 226 1 0.0 1 1 254 1 0.0 1 0 1 259 1 0.0 1 1 261 530 0.0 1 501 29 262 13 0.0 1 12 1