This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.32 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 293,172 (10.3%) Reads that were too short: 104,072 (3.6%) Reads written (passing filters): 189,100 (6.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 50,513,696 bp (5.9%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 293172 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1933 696.4 0 1933 7 75248 696.4 0 75248 8 1012 696.4 0 1012 9 71090 696.4 0 71090 10 80 696.4 0 80 11 26 696.4 0 26 12 18 696.4 0 18 13 27 696.4 0 27 14 31 696.4 0 31 15 42 696.4 0 42 16 44 696.4 0 44 17 37 696.4 0 37 18 33 696.4 0 33 19 33 696.4 0 33 20 25 696.4 0 25 21 20 696.4 0 20 22 31 696.4 0 31 23 20 696.4 0 20 24 34 696.4 0 34 25 24 696.4 0 24 26 32 696.4 0 32 27 523 696.4 0 523 28 67 696.4 0 67 29 25 696.4 0 25 30 31 696.4 0 31 31 26 696.4 0 26 32 28 696.4 0 28 33 32 696.4 0 32 34 28 696.4 0 28 35 38 696.4 0 38 36 30 696.4 0 30 37 28 696.4 0 28 38 24 696.4 0 24 39 35 696.4 0 35 40 38 696.4 0 38 41 34 696.4 0 34 42 55 696.4 0 55 43 34 696.4 0 34 44 42 696.4 0 42 45 27 696.4 0 27 46 30 696.4 0 30 47 29 696.4 0 29 48 32 696.4 0 32 49 33 696.4 0 33 50 60 696.4 0 60 51 34 696.4 0 34 52 36 696.4 0 36 53 31 696.4 0 31 54 65 696.4 0 65 55 76 696.4 0 76 56 99 696.4 0 99 57 1255 696.4 0 1255 58 1303 696.4 0 1303 59 757 696.4 0 757 60 31 696.4 0 31 61 28 696.4 0 28 62 28 696.4 0 28 63 33 696.4 0 33 64 41 696.4 0 41 65 30 696.4 0 30 66 23 696.4 0 23 67 32 696.4 0 32 68 27 696.4 0 27 69 21 696.4 0 21 70 20 696.4 0 20 71 21 696.4 0 21 72 35 696.4 0 35 73 21 696.4 0 21 74 25 696.4 0 25 75 20 696.4 0 20 76 21 696.4 0 21 77 34 696.4 0 34 78 43 696.4 0 43 79 70 696.4 0 70 80 85 696.4 0 85 81 62 696.4 0 62 82 43 696.4 0 43 83 63 696.4 0 63 84 87 696.4 0 87 85 50 696.4 0 50 86 80 696.4 0 80 87 74 696.4 0 74 88 220 696.4 0 220 89 127 696.4 0 127 90 206 696.4 0 206 91 115 696.4 0 115 92 137 696.4 0 137 93 67 696.4 0 67 94 60 696.4 0 60 95 58 696.4 0 58 96 63 696.4 0 63 97 43 696.4 0 43 98 63 696.4 0 63 99 71 696.4 0 71 100 58 696.4 0 58 101 65 696.4 0 65 102 68 696.4 0 68 103 68 696.4 0 68 104 94 696.4 0 94 105 147 696.4 0 147 106 124 696.4 0 124 107 101 696.4 0 101 108 99 696.4 0 99 109 84 696.4 0 84 110 64 696.4 0 64 111 42 696.4 0 42 112 56 696.4 0 56 113 56 696.4 0 56 114 77 696.4 0 77 115 96 696.4 0 96 116 62 696.4 0 62 117 79 696.4 0 79 118 50 696.4 0 50 119 63 696.4 0 63 120 79 696.4 0 79 121 154 696.4 0 154 122 787 696.4 0 787 123 546 696.4 0 546 124 456 696.4 0 456 125 86 696.4 0 86 126 62 696.4 0 62 127 133 696.4 0 133 128 3381 696.4 0 3381 129 78 696.4 0 78 130 117 696.4 0 117 131 110 696.4 0 110 132 73 696.4 0 73 133 58 696.4 0 58 134 90 696.4 0 90 135 122 696.4 0 122 136 71 696.4 0 71 137 74 696.4 0 74 138 75 696.4 0 75 139 72 696.4 0 72 140 85 696.4 0 85 141 322 696.4 0 322 142 363 696.4 0 363 143 257 696.4 0 257 144 94 696.4 0 94 145 92 696.4 0 92 146 133 696.4 0 133 147 206 696.4 0 206 148 231 696.4 0 231 149 324 696.4 0 324 150 315 696.4 0 315 151 4588 696.4 0 4588 152 6104 696.4 0 6104 153 4303 696.4 0 4303 154 124 696.4 0 124 155 106 696.4 0 106 156 93 696.4 0 93 157 420 696.4 0 420 158 249 696.4 0 249 159 249 696.4 0 249 160 81 696.4 0 81 161 141 696.4 0 141 162 258 696.4 0 258 163 162 696.4 0 162 164 92 696.4 0 92 165 68 696.4 0 68 166 77 696.4 0 77 167 100 696.4 0 100 168 95 696.4 0 95 169 92 696.4 0 92 170 152 696.4 0 152 171 102 696.4 0 102 172 105 696.4 0 105 173 40 696.4 0 40 174 75 696.4 0 75 175 102 696.4 0 102 176 166 696.4 0 166 177 98 696.4 0 98 178 78 696.4 0 78 179 86 696.4 0 86 180 68 696.4 0 68 181 85 696.4 0 85 182 254 696.4 0 254 183 200 696.4 0 200 184 163 696.4 0 163 185 90 696.4 0 90 186 197 696.4 0 197 187 190 696.4 0 190 188 155 696.4 0 155 189 120 696.4 0 120 190 70 696.4 0 70 191 69 696.4 0 69 192 51 696.4 0 51 193 74 696.4 0 74 194 119 696.4 0 119 195 171 696.4 0 171 196 234 696.4 0 234 197 132 696.4 0 132 198 105 696.4 0 105 199 73 696.4 0 73 200 100 696.4 0 100 201 172 696.4 0 172 202 140 696.4 0 140 203 110 696.4 0 110 204 113 696.4 0 113 205 156 696.4 0 156 206 167 696.4 0 167 207 209 696.4 0 209 208 387 696.4 0 387 209 869 696.4 0 869 210 1083 696.4 0 1083 211 1296 696.4 0 1296 212 1794 696.4 0 1794 213 23954 696.4 0 23954 214 38946 696.4 0 38946 215 18640 696.4 0 18640 216 450 696.4 0 450 217 133 696.4 0 133 218 114 696.4 0 114 219 116 696.4 0 116 220 263 696.4 0 263 221 1355 696.4 0 1355 222 1560 696.4 0 1560 223 1392 696.4 0 1392 224 156 696.4 0 156 225 87 696.4 0 87 226 90 696.4 0 90 227 84 696.4 0 84 228 78 696.4 0 78 229 68 696.4 0 68 230 57 696.4 0 57 231 111 696.4 0 111 232 113 696.4 0 113 233 125 696.4 0 125 234 111 696.4 0 111 235 107 696.4 0 107 236 86 696.4 0 86 237 73 696.4 0 73 238 75 696.4 0 75 239 70 696.4 0 70 240 86 696.4 0 86 241 85 696.4 0 85 242 101 696.4 0 101 243 125 696.4 0 125 244 178 696.4 0 178 245 149 696.4 0 149 246 105 696.4 0 105 247 73 696.4 0 73 248 64 696.4 0 64 249 86 696.4 0 86 250 88 696.4 0 88 251 79 696.4 0 79 252 76 696.4 0 76 253 103 696.4 0 103 254 91 696.4 0 91 255 125 696.4 0 125 256 133 696.4 0 133 257 344 696.4 0 344 258 404 696.4 0 404 259 360 696.4 0 360 260 186 696.4 0 186 261 194 696.4 0 194 262 175 696.4 0 175 263 528 696.4 0 528 264 254 696.4 0 254 265 208 696.4 0 208 266 114 696.4 0 114 267 63 696.4 0 63 268 86 696.4 0 86 269 176 696.4 0 176 270 249 696.4 0 249 271 161 696.4 0 161 272 148 696.4 0 148 273 184 696.4 0 184 274 124 696.4 0 124 275 165 696.4 0 165 276 175 696.4 0 175 277 159 696.4 0 159 278 129 696.4 0 129 279 104 696.4 0 104 280 90 696.4 0 90 281 204 696.4 0 204 282 177 696.4 0 177 283 229 696.4 0 229 284 179 696.4 0 179 285 159 696.4 0 159 286 149 696.4 0 149 287 83 696.4 0 83 288 86 696.4 0 86 289 104 696.4 0 104 290 156 696.4 0 156 291 146 696.4 0 146 292 96 696.4 0 96 293 85 696.4 0 85 294 111 696.4 0 111 295 135 696.4 0 135 296 157 696.4 0 157 297 140 696.4 0 140 298 129 696.4 0 129 299 110 696.4 0 110 300 97 696.4 0 97 301 75 696.4 0 75 Finished in 8.43 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 189,100 Reads with adapters: 73,256 (38.7%) Reads that were too short: 210 (0.1%) Reads written (passing filters): 73,046 (38.6%) Total basepairs processed: 50,513,696 bp Total written (filtered): 20,083,973 bp (39.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 73256 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 15 0.0 3 0 0 14 1 16 482 0.0 3 0 468 13 1 17 72028 0.0 3 70627 1350 49 2 18 181 0.0 3 9 164 7 1 19 46 0.0 3 3 0 38 5 20 246 0.0 3 144 3 0 99 21 2 0.0 3 2 28 2 0.0 3 2 29 1 0.0 3 1 31 1 0.0 3 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 76 1 0.0 3 1 85 1 0.0 3 1 94 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 125 1 0.0 3 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 47 0.0 3 0 0 0 47 164 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 216 3 0.0 3 0 0 0 3 217 152 0.0 3 0 0 2 150 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 1 269 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.62 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 73,046 Reads with adapters: 3,577 (4.9%) Reads written (passing filters): 73,046 (100.0%) Total basepairs processed: 20,083,973 bp Total written (filtered): 20,073,100 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3577 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.8% C: 7.2% G: 15.4% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3441 1141.3 0 3441 4 132 285.3 0 132 5 2 71.3 0 2 6 2 17.8 0 2