This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.71 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 342,450 (12.0%) Reads that were too short: 17,603 (0.6%) Reads written (passing filters): 324,847 (11.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 71,888,748 bp (8.4%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 342450 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 803 696.4 0 803 7 1750 696.4 0 1750 8 25654 696.4 0 25654 9 20527 696.4 0 20527 10 72 696.4 0 72 11 62 696.4 0 62 12 64 696.4 0 64 13 48 696.4 0 48 14 62 696.4 0 62 15 60 696.4 0 60 16 48 696.4 0 48 17 51 696.4 0 51 18 70 696.4 0 70 19 82 696.4 0 82 20 94 696.4 0 94 21 85 696.4 0 85 22 116 696.4 0 116 23 186 696.4 0 186 24 174 696.4 0 174 25 67 696.4 0 67 26 73 696.4 0 73 27 84 696.4 0 84 28 69 696.4 0 69 29 75 696.4 0 75 30 103 696.4 0 103 31 87 696.4 0 87 32 90 696.4 0 90 33 88 696.4 0 88 34 97 696.4 0 97 35 102 696.4 0 102 36 141 696.4 0 141 37 128 696.4 0 128 38 171 696.4 0 171 39 109 696.4 0 109 40 130 696.4 0 130 41 91 696.4 0 91 42 151 696.4 0 151 43 95 696.4 0 95 44 119 696.4 0 119 45 199 696.4 0 199 46 225 696.4 0 225 47 363 696.4 0 363 48 703 696.4 0 703 49 1461 696.4 0 1461 50 415 696.4 0 415 51 532 696.4 0 532 52 988 696.4 0 988 53 541 696.4 0 541 54 498 696.4 0 498 55 748 696.4 0 748 56 893 696.4 0 893 57 996 696.4 0 996 58 714 696.4 0 714 59 1513 696.4 0 1513 60 2034 696.4 0 2034 61 3824 696.4 0 3824 62 57772 696.4 0 57772 63 51625 696.4 0 51625 64 51230 696.4 0 51230 65 1779 696.4 0 1779 66 80 696.4 0 80 67 57 696.4 0 57 68 67 696.4 0 67 69 80 696.4 0 80 70 95 696.4 0 95 71 390 696.4 0 390 72 513 696.4 0 513 73 328 696.4 0 328 74 140 696.4 0 140 75 186 696.4 0 186 76 1428 696.4 0 1428 77 1267 696.4 0 1267 78 853 696.4 0 853 79 65 696.4 0 65 80 94 696.4 0 94 81 66 696.4 0 66 82 71 696.4 0 71 83 113 696.4 0 113 84 50 696.4 0 50 85 88 696.4 0 88 86 71 696.4 0 71 87 81 696.4 0 81 88 54 696.4 0 54 89 65 696.4 0 65 90 65 696.4 0 65 91 88 696.4 0 88 92 72 696.4 0 72 93 126 696.4 0 126 94 83 696.4 0 83 95 65 696.4 0 65 96 50 696.4 0 50 97 45 696.4 0 45 98 83 696.4 0 83 99 71 696.4 0 71 100 63 696.4 0 63 101 55 696.4 0 55 102 65 696.4 0 65 103 84 696.4 0 84 104 93 696.4 0 93 105 139 696.4 0 139 106 101 696.4 0 101 107 440 696.4 0 440 108 464 696.4 0 464 109 499 696.4 0 499 110 233 696.4 0 233 111 139 696.4 0 139 112 181 696.4 0 181 113 58 696.4 0 58 114 68 696.4 0 68 115 55 696.4 0 55 116 67 696.4 0 67 117 98 696.4 0 98 118 70 696.4 0 70 119 65 696.4 0 65 120 69 696.4 0 69 121 77 696.4 0 77 122 53 696.4 0 53 123 52 696.4 0 52 124 72 696.4 0 72 125 91 696.4 0 91 126 156 696.4 0 156 127 270 696.4 0 270 128 169 696.4 0 169 129 98 696.4 0 98 130 86 696.4 0 86 131 107 696.4 0 107 132 89 696.4 0 89 133 95 696.4 0 95 134 107 696.4 0 107 135 102 696.4 0 102 136 73 696.4 0 73 137 143 696.4 0 143 138 134 696.4 0 134 139 201 696.4 0 201 140 201 696.4 0 201 141 288 696.4 0 288 142 532 696.4 0 532 143 1101 696.4 0 1101 144 11642 696.4 0 11642 145 10234 696.4 0 10234 146 5485 696.4 0 5485 147 192 696.4 0 192 148 91 696.4 0 91 149 168 696.4 0 168 150 97 696.4 0 97 151 156 696.4 0 156 152 156 696.4 0 156 153 124 696.4 0 124 154 185 696.4 0 185 155 223 696.4 0 223 156 2491 696.4 0 2491 157 1695 696.4 0 1695 158 1135 696.4 0 1135 159 219 696.4 0 219 160 218 696.4 0 218 161 290 696.4 0 290 162 463 696.4 0 463 163 713 696.4 0 713 164 6404 696.4 0 6404 165 6063 696.4 0 6063 166 9916 696.4 0 9916 167 264 696.4 0 264 168 193 696.4 0 193 169 1559 696.4 0 1559 170 2216 696.4 0 2216 171 2853 696.4 0 2853 172 2884 696.4 0 2884 173 973 696.4 0 973 174 642 696.4 0 642 175 130 696.4 0 130 176 70 696.4 0 70 177 127 696.4 0 127 178 116 696.4 0 116 179 251 696.4 0 251 180 162 696.4 0 162 181 190 696.4 0 190 182 125 696.4 0 125 183 122 696.4 0 122 184 249 696.4 0 249 185 244 696.4 0 244 186 313 696.4 0 313 187 134 696.4 0 134 188 225 696.4 0 225 189 1437 696.4 0 1437 190 2023 696.4 0 2023 191 1829 696.4 0 1829 192 604 696.4 0 604 193 72 696.4 0 72 194 70 696.4 0 70 195 55 696.4 0 55 196 115 696.4 0 115 197 157 696.4 0 157 198 238 696.4 0 238 199 119 696.4 0 119 200 80 696.4 0 80 201 62 696.4 0 62 202 73 696.4 0 73 203 103 696.4 0 103 204 95 696.4 0 95 205 88 696.4 0 88 206 127 696.4 0 127 207 94 696.4 0 94 208 131 696.4 0 131 209 90 696.4 0 90 210 63 696.4 0 63 211 69 696.4 0 69 212 112 696.4 0 112 213 123 696.4 0 123 214 120 696.4 0 120 215 212 696.4 0 212 216 292 696.4 0 292 217 317 696.4 0 317 218 275 696.4 0 275 219 88 696.4 0 88 220 77 696.4 0 77 221 83 696.4 0 83 222 58 696.4 0 58 223 92 696.4 0 92 224 116 696.4 0 116 225 228 696.4 0 228 226 223 696.4 0 223 227 193 696.4 0 193 228 187 696.4 0 187 229 513 696.4 0 513 230 379 696.4 0 379 231 181 696.4 0 181 232 85 696.4 0 85 233 81 696.4 0 81 234 73 696.4 0 73 235 81 696.4 0 81 236 124 696.4 0 124 237 133 696.4 0 133 238 224 696.4 0 224 239 126 696.4 0 126 240 95 696.4 0 95 241 65 696.4 0 65 242 70 696.4 0 70 243 108 696.4 0 108 244 106 696.4 0 106 245 196 696.4 0 196 246 176 696.4 0 176 247 256 696.4 0 256 248 174 696.4 0 174 249 97 696.4 0 97 250 78 696.4 0 78 251 101 696.4 0 101 252 98 696.4 0 98 253 76 696.4 0 76 254 96 696.4 0 96 255 68 696.4 0 68 256 80 696.4 0 80 257 79 696.4 0 79 258 90 696.4 0 90 259 170 696.4 0 170 260 378 696.4 0 378 261 474 696.4 0 474 262 407 696.4 0 407 263 237 696.4 0 237 264 206 696.4 0 206 265 170 696.4 0 170 266 244 696.4 0 244 267 180 696.4 0 180 268 147 696.4 0 147 269 66 696.4 0 66 270 92 696.4 0 92 271 138 696.4 0 138 272 127 696.4 0 127 273 129 696.4 0 129 274 115 696.4 0 115 275 98 696.4 0 98 276 94 696.4 0 94 277 225 696.4 0 225 278 212 696.4 0 212 279 170 696.4 0 170 280 184 696.4 0 184 281 130 696.4 0 130 282 98 696.4 0 98 283 79 696.4 0 79 284 205 696.4 0 205 285 275 696.4 0 275 286 381 696.4 0 381 287 1034 696.4 0 1034 288 902 696.4 0 902 289 620 696.4 0 620 290 226 696.4 0 226 291 125 696.4 0 125 292 117 696.4 0 117 293 184 696.4 0 184 294 113 696.4 0 113 295 156 696.4 0 156 296 178 696.4 0 178 297 154 696.4 0 154 298 180 696.4 0 180 299 148 696.4 0 148 300 82 696.4 0 82 301 115 696.4 0 115 Finished in 14.22 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 324,847 Reads with adapters: 36,471 (11.2%) Reads that were too short: 331 (0.1%) Reads written (passing filters): 36,140 (11.1%) Total basepairs processed: 71,888,748 bp Total written (filtered): 8,408,384 bp (11.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 36471 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 21 0.0 3 0 0 12 7 2 19 272 0.0 3 0 229 17 18 8 20 22154 0.0 4 20811 1085 194 42 22 21 52 0.0 4 7 42 0 1 2 22 4 0.0 4 0 0 3 0 1 23 129 0.0 4 0 0 0 95 34 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 1 30 1 0.0 4 1 32 1 0.0 4 0 0 1 34 65 0.0 4 0 0 0 0 65 35 2 0.0 4 0 0 0 0 2 36 1 0.0 4 0 0 0 0 1 37 3 0.0 4 0 0 0 0 3 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 51 3 0.0 4 0 0 0 0 3 52 6 0.0 4 0 0 0 1 5 53 3 0.0 4 0 0 0 0 3 59 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 70 21 0.0 4 0 0 0 0 21 71 28 0.0 4 0 0 0 0 28 72 13073 0.0 4 0 0 0 0 13073 75 19 0.0 4 0 0 0 0 19 76 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 80 3 0.0 4 0 0 0 0 3 85 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 93 1 0.0 4 1 94 1 0.0 4 1 95 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 102 3 0.0 4 0 0 0 0 3 110 1 0.0 4 0 0 0 0 1 112 3 0.0 4 0 0 0 0 3 113 2 0.0 4 0 0 0 0 2 121 1 0.0 4 1 122 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 260 0.0 4 0 0 0 0 260 128 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 154 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 1 164 1 0.0 4 0 0 0 0 1 166 6 0.0 4 0 0 0 0 6 167 2 0.0 4 0 0 0 0 2 168 4 0.0 4 0 0 0 0 4 170 8 0.0 4 0 0 0 0 8 171 3 0.0 4 0 0 0 0 3 172 1 0.0 4 0 0 0 0 1 173 112 0.0 4 0 0 0 4 108 174 23 0.0 4 0 0 0 2 21 175 5 0.0 4 0 0 0 0 5 177 4 0.0 4 0 0 0 0 4 178 1 0.0 4 0 0 0 0 1 179 7 0.0 4 0 0 0 0 7 180 7 0.0 4 0 0 0 1 6 181 34 0.0 4 0 0 0 0 34 183 3 0.0 4 0 0 0 0 3 199 1 0.0 4 0 0 0 0 1 201 2 0.0 4 0 0 0 0 2 202 4 0.0 4 0 0 0 0 4 203 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 222 2 0.0 4 0 0 0 0 2 223 1 0.0 4 0 0 0 0 1 227 13 0.0 4 0 0 0 1 12 228 10 0.0 4 0 0 0 0 10 229 3 0.0 4 0 0 0 0 3 230 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 233 8 0.0 4 0 0 0 0 8 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 236 4 0.0 4 0 0 0 0 4 237 4 0.0 4 0 0 0 0 4 238 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 1 1 264 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.19 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 36,140 Reads with adapters: 6,098 (16.9%) Reads written (passing filters): 36,140 (100.0%) Total basepairs processed: 8,408,384 bp Total written (filtered): 8,347,102 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6098 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 7.8% G: 86.0% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 43 564.7 0 43 4 97 141.2 0 97 5 4 35.3 0 4 6 2 8.8 0 2 7 3 2.2 0 3 8 5 0.6 0 5 9 1930 0.1 0 228 1702 10 3041 0.0 1 234 2807 11 926 0.0 1 43 883 12 18 0.0 1 0 18 13 14 0.0 1 0 14 14 3 0.0 1 0 3 18 1 0.0 1 0 1 150 1 0.0 1 1 208 8 0.0 1 4 4 211 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.