This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.49 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 303,909 (10.7%) Reads that were too short: 9,632 (0.3%) Reads written (passing filters): 294,277 (10.3%) Total basepairs processed: 858,556,748 bp Total written (filtered): 66,934,980 bp (7.8%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 303909 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 738 696.4 0 738 7 1327 696.4 0 1327 8 24032 696.4 0 24032 9 24467 696.4 0 24467 10 61 696.4 0 61 11 58 696.4 0 58 12 49 696.4 0 49 13 41 696.4 0 41 14 40 696.4 0 40 15 42 696.4 0 42 16 34 696.4 0 34 17 50 696.4 0 50 18 68 696.4 0 68 19 63 696.4 0 63 20 68 696.4 0 68 21 50 696.4 0 50 22 70 696.4 0 70 23 80 696.4 0 80 24 84 696.4 0 84 25 31 696.4 0 31 26 52 696.4 0 52 27 51 696.4 0 51 28 54 696.4 0 54 29 377 696.4 0 377 30 121 696.4 0 121 31 54 696.4 0 54 32 57 696.4 0 57 33 62 696.4 0 62 34 49 696.4 0 49 35 63 696.4 0 63 36 105 696.4 0 105 37 97 696.4 0 97 38 151 696.4 0 151 39 100 696.4 0 100 40 96 696.4 0 96 41 56 696.4 0 56 42 122 696.4 0 122 43 95 696.4 0 95 44 95 696.4 0 95 45 149 696.4 0 149 46 222 696.4 0 222 47 353 696.4 0 353 48 662 696.4 0 662 49 1332 696.4 0 1332 50 371 696.4 0 371 51 447 696.4 0 447 52 893 696.4 0 893 53 322 696.4 0 322 54 367 696.4 0 367 55 677 696.4 0 677 56 806 696.4 0 806 57 840 696.4 0 840 58 604 696.4 0 604 59 1305 696.4 0 1305 60 1631 696.4 0 1631 61 2717 696.4 0 2717 62 54239 696.4 0 54239 63 49613 696.4 0 49613 64 51127 696.4 0 51127 65 1736 696.4 0 1736 66 47 696.4 0 47 67 41 696.4 0 41 68 43 696.4 0 43 69 35 696.4 0 35 70 55 696.4 0 55 71 224 696.4 0 224 72 377 696.4 0 377 73 190 696.4 0 190 74 79 696.4 0 79 75 126 696.4 0 126 76 1768 696.4 0 1768 77 1426 696.4 0 1426 78 936 696.4 0 936 79 61 696.4 0 61 80 64 696.4 0 64 81 31 696.4 0 31 82 43 696.4 0 43 83 61 696.4 0 61 84 38 696.4 0 38 85 56 696.4 0 56 86 45 696.4 0 45 87 53 696.4 0 53 88 51 696.4 0 51 89 50 696.4 0 50 90 45 696.4 0 45 91 48 696.4 0 48 92 42 696.4 0 42 93 57 696.4 0 57 94 43 696.4 0 43 95 57 696.4 0 57 96 42 696.4 0 42 97 41 696.4 0 41 98 58 696.4 0 58 99 37 696.4 0 37 100 42 696.4 0 42 101 33 696.4 0 33 102 36 696.4 0 36 103 61 696.4 0 61 104 52 696.4 0 52 105 94 696.4 0 94 106 53 696.4 0 53 107 191 696.4 0 191 108 140 696.4 0 140 109 169 696.4 0 169 110 105 696.4 0 105 111 70 696.4 0 70 112 105 696.4 0 105 113 50 696.4 0 50 114 39 696.4 0 39 115 56 696.4 0 56 116 59 696.4 0 59 117 80 696.4 0 80 118 50 696.4 0 50 119 50 696.4 0 50 120 50 696.4 0 50 121 98 696.4 0 98 122 56 696.4 0 56 123 26 696.4 0 26 124 70 696.4 0 70 125 62 696.4 0 62 126 121 696.4 0 121 127 180 696.4 0 180 128 119 696.4 0 119 129 84 696.4 0 84 130 76 696.4 0 76 131 61 696.4 0 61 132 82 696.4 0 82 133 60 696.4 0 60 134 73 696.4 0 73 135 56 696.4 0 56 136 58 696.4 0 58 137 94 696.4 0 94 138 68 696.4 0 68 139 110 696.4 0 110 140 139 696.4 0 139 141 181 696.4 0 181 142 361 696.4 0 361 143 572 696.4 0 572 144 10082 696.4 0 10082 145 9119 696.4 0 9119 146 5067 696.4 0 5067 147 158 696.4 0 158 148 83 696.4 0 83 149 99 696.4 0 99 150 70 696.4 0 70 151 89 696.4 0 89 152 102 696.4 0 102 153 84 696.4 0 84 154 127 696.4 0 127 155 151 696.4 0 151 156 1235 696.4 0 1235 157 934 696.4 0 934 158 596 696.4 0 596 159 152 696.4 0 152 160 166 696.4 0 166 161 223 696.4 0 223 162 387 696.4 0 387 163 457 696.4 0 457 164 5916 696.4 0 5916 165 5817 696.4 0 5817 166 10686 696.4 0 10686 167 127 696.4 0 127 168 84 696.4 0 84 169 628 696.4 0 628 170 637 696.4 0 637 171 1135 696.4 0 1135 172 995 696.4 0 995 173 384 696.4 0 384 174 250 696.4 0 250 175 79 696.4 0 79 176 68 696.4 0 68 177 58 696.4 0 58 178 63 696.4 0 63 179 148 696.4 0 148 180 145 696.4 0 145 181 128 696.4 0 128 182 69 696.4 0 69 183 61 696.4 0 61 184 125 696.4 0 125 185 93 696.4 0 93 186 125 696.4 0 125 187 61 696.4 0 61 188 85 696.4 0 85 189 458 696.4 0 458 190 629 696.4 0 629 191 570 696.4 0 570 192 273 696.4 0 273 193 50 696.4 0 50 194 31 696.4 0 31 195 37 696.4 0 37 196 90 696.4 0 90 197 58 696.4 0 58 198 81 696.4 0 81 199 67 696.4 0 67 200 61 696.4 0 61 201 39 696.4 0 39 202 52 696.4 0 52 203 65 696.4 0 65 204 65 696.4 0 65 205 54 696.4 0 54 206 54 696.4 0 54 207 47 696.4 0 47 208 62 696.4 0 62 209 69 696.4 0 69 210 48 696.4 0 48 211 50 696.4 0 50 212 70 696.4 0 70 213 69 696.4 0 69 214 79 696.4 0 79 215 111 696.4 0 111 216 127 696.4 0 127 217 168 696.4 0 168 218 136 696.4 0 136 219 59 696.4 0 59 220 46 696.4 0 46 221 39 696.4 0 39 222 39 696.4 0 39 223 47 696.4 0 47 224 76 696.4 0 76 225 233 696.4 0 233 226 107 696.4 0 107 227 142 696.4 0 142 228 102 696.4 0 102 229 548 696.4 0 548 230 329 696.4 0 329 231 143 696.4 0 143 232 62 696.4 0 62 233 72 696.4 0 72 234 50 696.4 0 50 235 42 696.4 0 42 236 81 696.4 0 81 237 96 696.4 0 96 238 101 696.4 0 101 239 62 696.4 0 62 240 53 696.4 0 53 241 55 696.4 0 55 242 33 696.4 0 33 243 63 696.4 0 63 244 74 696.4 0 74 245 64 696.4 0 64 246 61 696.4 0 61 247 78 696.4 0 78 248 55 696.4 0 55 249 52 696.4 0 52 250 50 696.4 0 50 251 53 696.4 0 53 252 56 696.4 0 56 253 40 696.4 0 40 254 46 696.4 0 46 255 49 696.4 0 49 256 36 696.4 0 36 257 47 696.4 0 47 258 48 696.4 0 48 259 59 696.4 0 59 260 122 696.4 0 122 261 161 696.4 0 161 262 168 696.4 0 168 263 129 696.4 0 129 264 81 696.4 0 81 265 81 696.4 0 81 266 95 696.4 0 95 267 95 696.4 0 95 268 80 696.4 0 80 269 50 696.4 0 50 270 57 696.4 0 57 271 84 696.4 0 84 272 57 696.4 0 57 273 78 696.4 0 78 274 80 696.4 0 80 275 72 696.4 0 72 276 58 696.4 0 58 277 144 696.4 0 144 278 119 696.4 0 119 279 77 696.4 0 77 280 77 696.4 0 77 281 85 696.4 0 85 282 47 696.4 0 47 283 58 696.4 0 58 284 65 696.4 0 65 285 126 696.4 0 126 286 161 696.4 0 161 287 518 696.4 0 518 288 457 696.4 0 457 289 306 696.4 0 306 290 133 696.4 0 133 291 66 696.4 0 66 292 59 696.4 0 59 293 71 696.4 0 71 294 55 696.4 0 55 295 127 696.4 0 127 296 115 696.4 0 115 297 72 696.4 0 72 298 76 696.4 0 76 299 66 696.4 0 66 300 58 696.4 0 58 301 72 696.4 0 72 Finished in 11.59 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 294,277 Reads with adapters: 24,164 (8.2%) Reads that were too short: 107 (0.0%) Reads written (passing filters): 24,057 (8.2%) Total basepairs processed: 66,934,980 bp Total written (filtered): 6,583,157 bp (9.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24164 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 6 16 162 0.0 3 0 160 2 17 23399 0.0 3 22923 460 15 1 18 55 0.0 3 3 48 4 19 37 0.0 3 23 2 9 3 20 44 0.0 3 0 0 0 44 22 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 8 0.0 3 0 0 0 8 29 3 0.0 3 1 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 1 0.0 3 1 60 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 84 6 0.0 3 0 0 0 6 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 1 2 107 5 0.0 3 0 0 0 5 108 1 0.0 3 0 0 0 1 109 9 0.0 3 0 0 0 9 111 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 114 4 0.0 3 0 0 0 4 115 1 0.0 3 0 0 0 1 117 4 0.0 3 0 0 0 4 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 6 0.0 3 0 0 0 6 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 6 0.0 3 0 0 0 6 129 231 0.0 3 0 0 0 231 130 18 0.0 3 0 0 0 18 131 2 0.0 3 0 0 0 2 132 28 0.0 3 0 0 0 28 133 7 0.0 3 0 0 0 7 144 4 0.0 3 0 0 0 4 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 5 0.0 3 0 0 0 5 181 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 230 8 0.0 3 0 0 0 8 231 23 0.0 3 0 0 0 23 232 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 24,057 Reads with adapters: 224 (0.9%) Reads written (passing filters): 24,057 (100.0%) Total basepairs processed: 6,583,157 bp Total written (filtered): 6,582,336 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 224 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.3% C: 3.1% G: 50.0% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 75 375.9 0 75 4 149 94.0 0 149