This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.63 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 151,965 (5.3%) Reads that were too short: 10,277 (0.4%) Reads written (passing filters): 141,688 (5.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 38,131,935 bp (4.4%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 151965 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2764 696.4 0 2764 7 61927 696.4 0 61927 8 49219 696.4 0 49219 9 112 696.4 0 112 10 69 696.4 0 69 11 54 696.4 0 54 12 124 696.4 0 124 13 53 696.4 0 53 14 28 696.4 0 28 15 51 696.4 0 51 16 69 696.4 0 69 17 54 696.4 0 54 18 88 696.4 0 88 19 122 696.4 0 122 20 115 696.4 0 115 21 128 696.4 0 128 22 44 696.4 0 44 23 53 696.4 0 53 24 44 696.4 0 44 25 37 696.4 0 37 26 79 696.4 0 79 27 47 696.4 0 47 28 78 696.4 0 78 29 87 696.4 0 87 30 60 696.4 0 60 31 60 696.4 0 60 32 51 696.4 0 51 33 49 696.4 0 49 34 61 696.4 0 61 35 68 696.4 0 68 36 64 696.4 0 64 37 59 696.4 0 59 38 152 696.4 0 152 39 114 696.4 0 114 40 139 696.4 0 139 41 212 696.4 0 212 42 69 696.4 0 69 43 46 696.4 0 46 44 66 696.4 0 66 45 54 696.4 0 54 46 70 696.4 0 70 47 64 696.4 0 64 48 58 696.4 0 58 49 58 696.4 0 58 50 60 696.4 0 60 51 53 696.4 0 53 52 55 696.4 0 55 53 53 696.4 0 53 54 36 696.4 0 36 55 66 696.4 0 66 56 64 696.4 0 64 57 79 696.4 0 79 58 55 696.4 0 55 59 61 696.4 0 61 60 72 696.4 0 72 61 53 696.4 0 53 62 61 696.4 0 61 63 66 696.4 0 66 64 47 696.4 0 47 65 71 696.4 0 71 66 69 696.4 0 69 67 52 696.4 0 52 68 60 696.4 0 60 69 60 696.4 0 60 70 53 696.4 0 53 71 63 696.4 0 63 72 56 696.4 0 56 73 81 696.4 0 81 74 66 696.4 0 66 75 75 696.4 0 75 76 268 696.4 0 268 77 202 696.4 0 202 78 139 696.4 0 139 79 85 696.4 0 85 80 74 696.4 0 74 81 131 696.4 0 131 82 64 696.4 0 64 83 74 696.4 0 74 84 91 696.4 0 91 85 75 696.4 0 75 86 66 696.4 0 66 87 72 696.4 0 72 88 61 696.4 0 61 89 56 696.4 0 56 90 67 696.4 0 67 91 110 696.4 0 110 92 103 696.4 0 103 93 337 696.4 0 337 94 310 696.4 0 310 95 330 696.4 0 330 96 325 696.4 0 325 97 294 696.4 0 294 98 208 696.4 0 208 99 62 696.4 0 62 100 41 696.4 0 41 101 52 696.4 0 52 102 46 696.4 0 46 103 48 696.4 0 48 104 46 696.4 0 46 105 58 696.4 0 58 106 61 696.4 0 61 107 57 696.4 0 57 108 61 696.4 0 61 109 51 696.4 0 51 110 69 696.4 0 69 111 61 696.4 0 61 112 46 696.4 0 46 113 69 696.4 0 69 114 48 696.4 0 48 115 58 696.4 0 58 116 72 696.4 0 72 117 57 696.4 0 57 118 50 696.4 0 50 119 47 696.4 0 47 120 37 696.4 0 37 121 41 696.4 0 41 122 62 696.4 0 62 123 44 696.4 0 44 124 43 696.4 0 43 125 52 696.4 0 52 126 43 696.4 0 43 127 61 696.4 0 61 128 67 696.4 0 67 129 123 696.4 0 123 130 166 696.4 0 166 131 423 696.4 0 423 132 282 696.4 0 282 133 118 696.4 0 118 134 133 696.4 0 133 135 112 696.4 0 112 136 105 696.4 0 105 137 72 696.4 0 72 138 63 696.4 0 63 139 59 696.4 0 59 140 60 696.4 0 60 141 46 696.4 0 46 142 69 696.4 0 69 143 93 696.4 0 93 144 122 696.4 0 122 145 112 696.4 0 112 146 52 696.4 0 52 147 48 696.4 0 48 148 58 696.4 0 58 149 53 696.4 0 53 150 60 696.4 0 60 151 82 696.4 0 82 152 81 696.4 0 81 153 58 696.4 0 58 154 74 696.4 0 74 155 56 696.4 0 56 156 79 696.4 0 79 157 73 696.4 0 73 158 63 696.4 0 63 159 58 696.4 0 58 160 51 696.4 0 51 161 59 696.4 0 59 162 72 696.4 0 72 163 57 696.4 0 57 164 88 696.4 0 88 165 114 696.4 0 114 166 136 696.4 0 136 167 280 696.4 0 280 168 278 696.4 0 278 169 2857 696.4 0 2857 170 2929 696.4 0 2929 171 6242 696.4 0 6242 172 88 696.4 0 88 173 53 696.4 0 53 174 92 696.4 0 92 175 50 696.4 0 50 176 44 696.4 0 44 177 35 696.4 0 35 178 46 696.4 0 46 179 55 696.4 0 55 180 60 696.4 0 60 181 67 696.4 0 67 182 50 696.4 0 50 183 52 696.4 0 52 184 47 696.4 0 47 185 64 696.4 0 64 186 64 696.4 0 64 187 39 696.4 0 39 188 49 696.4 0 49 189 54 696.4 0 54 190 57 696.4 0 57 191 48 696.4 0 48 192 71 696.4 0 71 193 58 696.4 0 58 194 86 696.4 0 86 195 46 696.4 0 46 196 59 696.4 0 59 197 47 696.4 0 47 198 64 696.4 0 64 199 55 696.4 0 55 200 54 696.4 0 54 201 59 696.4 0 59 202 67 696.4 0 67 203 138 696.4 0 138 204 115 696.4 0 115 205 93 696.4 0 93 206 74 696.4 0 74 207 46 696.4 0 46 208 46 696.4 0 46 209 68 696.4 0 68 210 82 696.4 0 82 211 70 696.4 0 70 212 67 696.4 0 67 213 87 696.4 0 87 214 56 696.4 0 56 215 75 696.4 0 75 216 75 696.4 0 75 217 111 696.4 0 111 218 87 696.4 0 87 219 71 696.4 0 71 220 39 696.4 0 39 221 68 696.4 0 68 222 105 696.4 0 105 223 76 696.4 0 76 224 104 696.4 0 104 225 50 696.4 0 50 226 52 696.4 0 52 227 53 696.4 0 53 228 63 696.4 0 63 229 54 696.4 0 54 230 146 696.4 0 146 231 103 696.4 0 103 232 197 696.4 0 197 233 105 696.4 0 105 234 101 696.4 0 101 235 180 696.4 0 180 236 234 696.4 0 234 237 249 696.4 0 249 238 183 696.4 0 183 239 71 696.4 0 71 240 77 696.4 0 77 241 71 696.4 0 71 242 99 696.4 0 99 243 113 696.4 0 113 244 53 696.4 0 53 245 43 696.4 0 43 246 82 696.4 0 82 247 50 696.4 0 50 248 51 696.4 0 51 249 71 696.4 0 71 250 89 696.4 0 89 251 68 696.4 0 68 252 63 696.4 0 63 253 65 696.4 0 65 254 135 696.4 0 135 255 116 696.4 0 116 256 127 696.4 0 127 257 64 696.4 0 64 258 79 696.4 0 79 259 51 696.4 0 51 260 61 696.4 0 61 261 85 696.4 0 85 262 125 696.4 0 125 263 103 696.4 0 103 264 84 696.4 0 84 265 66 696.4 0 66 266 80 696.4 0 80 267 59 696.4 0 59 268 56 696.4 0 56 269 69 696.4 0 69 270 264 696.4 0 264 271 250 696.4 0 250 272 264 696.4 0 264 273 177 696.4 0 177 274 202 696.4 0 202 275 60 696.4 0 60 276 74 696.4 0 74 277 121 696.4 0 121 278 163 696.4 0 163 279 146 696.4 0 146 280 87 696.4 0 87 281 81 696.4 0 81 282 53 696.4 0 53 283 93 696.4 0 93 284 71 696.4 0 71 285 89 696.4 0 89 286 72 696.4 0 72 287 57 696.4 0 57 288 54 696.4 0 54 289 68 696.4 0 68 290 76 696.4 0 76 291 71 696.4 0 71 292 117 696.4 0 117 293 96 696.4 0 96 294 139 696.4 0 139 295 130 696.4 0 130 296 288 696.4 0 288 297 334 696.4 0 334 298 282 696.4 0 282 299 132 696.4 0 132 300 117 696.4 0 117 301 33 696.4 0 33 Finished in 7.39 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 141,688 Reads with adapters: 54,449 (38.4%) Reads that were too short: 64 (0.0%) Reads written (passing filters): 54,385 (38.4%) Total basepairs processed: 38,131,935 bp Total written (filtered): 14,802,351 bp (38.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 54449 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 3 3 18 28 0.0 3 0 0 17 7 4 19 648 0.0 3 0 507 73 49 19 20 52334 0.0 4 49138 2582 472 106 36 21 133 0.0 4 18 106 6 2 1 22 25 0.0 4 6 10 6 1 2 23 746 0.0 4 565 33 2 102 44 24 3 0.0 4 0 2 1 26 6 0.0 4 0 0 0 6 27 2 0.0 4 0 0 1 0 1 35 1 0.0 4 0 0 1 41 81 0.0 4 0 1 0 0 80 42 3 0.0 4 0 0 0 0 3 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 17 0.0 4 0 0 0 1 16 115 1 0.0 4 1 123 2 0.0 4 0 0 0 0 2 126 4 0.0 4 0 0 0 0 4 127 329 0.0 4 0 0 0 0 329 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 135 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 0 7 155 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 1 184 6 0.0 4 0 0 0 0 6 187 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 5 0.0 4 0 0 0 2 3 230 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 2 0.0 4 0 0 0 0 2 236 3 0.0 4 0 0 0 0 3 237 3 0.0 4 0 0 0 0 3 253 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 3 0.0 4 0 0 0 0 3 262 1 0.0 4 0 0 0 0 1 263 3 0.0 4 0 0 0 0 3 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.96 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 54,385 Reads with adapters: 8,521 (15.7%) Reads written (passing filters): 54,385 (100.0%) Total basepairs processed: 14,802,351 bp Total written (filtered): 14,700,843 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8521 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 10.5% G: 82.9% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 140 849.8 0 140 4 16 212.4 0 16 5 1 53.1 0 1 8 1 0.8 0 1 9 122 0.2 0 9 113 10 1994 0.1 1 178 1816 11 5976 0.0 1 297 5679 12 182 0.0 1 7 175 13 16 0.0 1 0 16 14 8 0.0 1 0 8 15 5 0.0 1 0 5 16 1 0.0 1 0 1 76 1 0.0 1 1 81 1 0.0 1 0 1 151 13 0.0 1 12 1 153 1 0.0 1 0 1 190 1 0.0 1 0 1 192 1 0.0 1 0 1 209 23 0.0 1 20 3 211 4 0.0 1 3 1 224 9 0.0 1 8 1 259 2 0.0 1 0 2 261 1 0.0 1 1 269 1 0.0 1 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.