This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.11 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 156,532 (5.5%) Reads that were too short: 8,544 (0.3%) Reads written (passing filters): 147,988 (5.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 39,547,968 bp (4.6%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 156532 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2475 696.4 0 2475 7 58851 696.4 0 58851 8 57179 696.4 0 57179 9 117 696.4 0 117 10 64 696.4 0 64 11 55 696.4 0 55 12 77 696.4 0 77 13 34 696.4 0 34 14 50 696.4 0 50 15 75 696.4 0 75 16 50 696.4 0 50 17 40 696.4 0 40 18 66 696.4 0 66 19 106 696.4 0 106 20 91 696.4 0 91 21 92 696.4 0 92 22 52 696.4 0 52 23 64 696.4 0 64 24 35 696.4 0 35 25 61 696.4 0 61 26 65 696.4 0 65 27 66 696.4 0 66 28 50 696.4 0 50 29 50 696.4 0 50 30 63 696.4 0 63 31 59 696.4 0 59 32 47 696.4 0 47 33 41 696.4 0 41 34 69 696.4 0 69 35 68 696.4 0 68 36 70 696.4 0 70 37 61 696.4 0 61 38 142 696.4 0 142 39 83 696.4 0 83 40 78 696.4 0 78 41 61 696.4 0 61 42 54 696.4 0 54 43 52 696.4 0 52 44 56 696.4 0 56 45 72 696.4 0 72 46 77 696.4 0 77 47 49 696.4 0 49 48 52 696.4 0 52 49 62 696.4 0 62 50 62 696.4 0 62 51 61 696.4 0 61 52 66 696.4 0 66 53 74 696.4 0 74 54 60 696.4 0 60 55 62 696.4 0 62 56 61 696.4 0 61 57 64 696.4 0 64 58 76 696.4 0 76 59 78 696.4 0 78 60 66 696.4 0 66 61 53 696.4 0 53 62 64 696.4 0 64 63 51 696.4 0 51 64 51 696.4 0 51 65 51 696.4 0 51 66 75 696.4 0 75 67 39 696.4 0 39 68 68 696.4 0 68 69 58 696.4 0 58 70 59 696.4 0 59 71 66 696.4 0 66 72 56 696.4 0 56 73 66 696.4 0 66 74 53 696.4 0 53 75 70 696.4 0 70 76 248 696.4 0 248 77 189 696.4 0 189 78 128 696.4 0 128 79 77 696.4 0 77 80 65 696.4 0 65 81 84 696.4 0 84 82 54 696.4 0 54 83 63 696.4 0 63 84 58 696.4 0 58 85 60 696.4 0 60 86 53 696.4 0 53 87 65 696.4 0 65 88 77 696.4 0 77 89 49 696.4 0 49 90 75 696.4 0 75 91 83 696.4 0 83 92 74 696.4 0 74 93 170 696.4 0 170 94 155 696.4 0 155 95 171 696.4 0 171 96 145 696.4 0 145 97 157 696.4 0 157 98 106 696.4 0 106 99 42 696.4 0 42 100 51 696.4 0 51 101 56 696.4 0 56 102 56 696.4 0 56 103 44 696.4 0 44 104 45 696.4 0 45 105 56 696.4 0 56 106 47 696.4 0 47 107 57 696.4 0 57 108 57 696.4 0 57 109 42 696.4 0 42 110 52 696.4 0 52 111 36 696.4 0 36 112 36 696.4 0 36 113 91 696.4 0 91 114 71 696.4 0 71 115 54 696.4 0 54 116 74 696.4 0 74 117 64 696.4 0 64 118 34 696.4 0 34 119 44 696.4 0 44 120 32 696.4 0 32 121 39 696.4 0 39 122 54 696.4 0 54 123 52 696.4 0 52 124 37 696.4 0 37 125 52 696.4 0 52 126 44 696.4 0 44 127 54 696.4 0 54 128 66 696.4 0 66 129 93 696.4 0 93 130 89 696.4 0 89 131 439 696.4 0 439 132 284 696.4 0 284 133 117 696.4 0 117 134 205 696.4 0 205 135 139 696.4 0 139 136 132 696.4 0 132 137 84 696.4 0 84 138 72 696.4 0 72 139 63 696.4 0 63 140 47 696.4 0 47 141 65 696.4 0 65 142 64 696.4 0 64 143 87 696.4 0 87 144 83 696.4 0 83 145 85 696.4 0 85 146 47 696.4 0 47 147 37 696.4 0 37 148 42 696.4 0 42 149 56 696.4 0 56 150 54 696.4 0 54 151 78 696.4 0 78 152 93 696.4 0 93 153 51 696.4 0 51 154 74 696.4 0 74 155 59 696.4 0 59 156 54 696.4 0 54 157 66 696.4 0 66 158 69 696.4 0 69 159 74 696.4 0 74 160 59 696.4 0 59 161 49 696.4 0 49 162 70 696.4 0 70 163 74 696.4 0 74 164 91 696.4 0 91 165 129 696.4 0 129 166 164 696.4 0 164 167 301 696.4 0 301 168 265 696.4 0 265 169 3569 696.4 0 3569 170 3948 696.4 0 3948 171 7778 696.4 0 7778 172 80 696.4 0 80 173 58 696.4 0 58 174 87 696.4 0 87 175 61 696.4 0 61 176 45 696.4 0 45 177 52 696.4 0 52 178 44 696.4 0 44 179 64 696.4 0 64 180 57 696.4 0 57 181 56 696.4 0 56 182 58 696.4 0 58 183 59 696.4 0 59 184 56 696.4 0 56 185 59 696.4 0 59 186 54 696.4 0 54 187 55 696.4 0 55 188 54 696.4 0 54 189 53 696.4 0 53 190 54 696.4 0 54 191 58 696.4 0 58 192 76 696.4 0 76 193 52 696.4 0 52 194 106 696.4 0 106 195 54 696.4 0 54 196 48 696.4 0 48 197 39 696.4 0 39 198 57 696.4 0 57 199 57 696.4 0 57 200 39 696.4 0 39 201 61 696.4 0 61 202 68 696.4 0 68 203 109 696.4 0 109 204 82 696.4 0 82 205 96 696.4 0 96 206 67 696.4 0 67 207 46 696.4 0 46 208 47 696.4 0 47 209 58 696.4 0 58 210 56 696.4 0 56 211 55 696.4 0 55 212 47 696.4 0 47 213 57 696.4 0 57 214 26 696.4 0 26 215 50 696.4 0 50 216 60 696.4 0 60 217 86 696.4 0 86 218 78 696.4 0 78 219 54 696.4 0 54 220 39 696.4 0 39 221 69 696.4 0 69 222 111 696.4 0 111 223 81 696.4 0 81 224 99 696.4 0 99 225 38 696.4 0 38 226 69 696.4 0 69 227 61 696.4 0 61 228 69 696.4 0 69 229 55 696.4 0 55 230 152 696.4 0 152 231 90 696.4 0 90 232 183 696.4 0 183 233 69 696.4 0 69 234 58 696.4 0 58 235 76 696.4 0 76 236 153 696.4 0 153 237 126 696.4 0 126 238 109 696.4 0 109 239 54 696.4 0 54 240 57 696.4 0 57 241 53 696.4 0 53 242 85 696.4 0 85 243 46 696.4 0 46 244 49 696.4 0 49 245 65 696.4 0 65 246 86 696.4 0 86 247 49 696.4 0 49 248 41 696.4 0 41 249 50 696.4 0 50 250 53 696.4 0 53 251 44 696.4 0 44 252 47 696.4 0 47 253 80 696.4 0 80 254 80 696.4 0 80 255 55 696.4 0 55 256 64 696.4 0 64 257 44 696.4 0 44 258 66 696.4 0 66 259 62 696.4 0 62 260 54 696.4 0 54 261 62 696.4 0 62 262 105 696.4 0 105 263 74 696.4 0 74 264 59 696.4 0 59 265 63 696.4 0 63 266 68 696.4 0 68 267 71 696.4 0 71 268 63 696.4 0 63 269 70 696.4 0 70 270 262 696.4 0 262 271 283 696.4 0 283 272 349 696.4 0 349 273 267 696.4 0 267 274 308 696.4 0 308 275 36 696.4 0 36 276 55 696.4 0 55 277 83 696.4 0 83 278 142 696.4 0 142 279 111 696.4 0 111 280 68 696.4 0 68 281 62 696.4 0 62 282 48 696.4 0 48 283 86 696.4 0 86 284 55 696.4 0 55 285 62 696.4 0 62 286 52 696.4 0 52 287 59 696.4 0 59 288 57 696.4 0 57 289 61 696.4 0 61 290 68 696.4 0 68 291 77 696.4 0 77 292 101 696.4 0 101 293 68 696.4 0 68 294 116 696.4 0 116 295 82 696.4 0 82 296 191 696.4 0 191 297 207 696.4 0 207 298 177 696.4 0 177 299 73 696.4 0 73 300 67 696.4 0 67 301 43 696.4 0 43 Finished in 6.84 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 147,988 Reads with adapters: 55,361 (37.4%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 55,335 (37.4%) Total basepairs processed: 39,547,968 bp Total written (filtered): 15,324,224 bp (38.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 55361 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 11 0.0 3 0 0 11 16 396 0.0 3 0 388 8 17 54636 0.0 3 53525 1078 31 2 18 157 0.0 3 9 139 9 19 16 0.0 3 1 0 14 1 20 89 0.0 3 0 1 0 88 21 2 0.0 3 1 0 0 1 22 1 0.0 3 1 26 1 0.0 3 1 35 1 0.0 3 1 39 1 0.0 3 1 42 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 53 8 0.0 3 0 0 0 8 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 1 126 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 194 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.96 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 55,335 Reads with adapters: 142 (0.3%) Reads written (passing filters): 55,335 (100.0%) Total basepairs processed: 15,324,224 bp Total written (filtered): 15,323,774 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 142 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.3% C: 31.7% G: 19.0% T: 12.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 123 864.6 0 123 4 14 216.2 0 14 5 5 54.0 0 5