This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.09 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 99,858 (3.5%) Reads that were too short: 8,978 (0.3%) Reads written (passing filters): 90,880 (3.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 25,167,591 bp (2.9%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 99858 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 995 696.4 0 995 7 1203 696.4 0 1203 8 43241 696.4 0 43241 9 33103 696.4 0 33103 10 83 696.4 0 83 11 88 696.4 0 88 12 14 696.4 0 14 13 27 696.4 0 27 14 21 696.4 0 21 15 31 696.4 0 31 16 18 696.4 0 18 17 8 696.4 0 8 18 19 696.4 0 19 19 17 696.4 0 17 20 18 696.4 0 18 21 34 696.4 0 34 22 29 696.4 0 29 23 17 696.4 0 17 24 14 696.4 0 14 25 16 696.4 0 16 26 11 696.4 0 11 27 20 696.4 0 20 28 16 696.4 0 16 29 8 696.4 0 8 30 21 696.4 0 21 31 24 696.4 0 24 32 7 696.4 0 7 33 34 696.4 0 34 34 20 696.4 0 20 35 52 696.4 0 52 36 21 696.4 0 21 37 35 696.4 0 35 38 45 696.4 0 45 39 15 696.4 0 15 40 16 696.4 0 16 41 41 696.4 0 41 42 9 696.4 0 9 43 24 696.4 0 24 44 21 696.4 0 21 45 22 696.4 0 22 46 34 696.4 0 34 47 364 696.4 0 364 48 411 696.4 0 411 49 363 696.4 0 363 50 696 696.4 0 696 51 50 696.4 0 50 52 15 696.4 0 15 53 11 696.4 0 11 54 10 696.4 0 10 55 12 696.4 0 12 56 16 696.4 0 16 57 13 696.4 0 13 58 8 696.4 0 8 59 18 696.4 0 18 60 19 696.4 0 19 61 11 696.4 0 11 62 31 696.4 0 31 63 9 696.4 0 9 64 10 696.4 0 10 65 15 696.4 0 15 66 7 696.4 0 7 67 16 696.4 0 16 68 4 696.4 0 4 69 10 696.4 0 10 70 11 696.4 0 11 71 14 696.4 0 14 72 7 696.4 0 7 73 14 696.4 0 14 74 25 696.4 0 25 75 22 696.4 0 22 76 20 696.4 0 20 77 17 696.4 0 17 78 31 696.4 0 31 79 11 696.4 0 11 80 28 696.4 0 28 81 77 696.4 0 77 82 118 696.4 0 118 83 81 696.4 0 81 84 57 696.4 0 57 85 26 696.4 0 26 86 23 696.4 0 23 87 186 696.4 0 186 88 45 696.4 0 45 89 38 696.4 0 38 90 100 696.4 0 100 91 36 696.4 0 36 92 17 696.4 0 17 93 32 696.4 0 32 94 15 696.4 0 15 95 20 696.4 0 20 96 36 696.4 0 36 97 81 696.4 0 81 98 88 696.4 0 88 99 15 696.4 0 15 100 11 696.4 0 11 101 10 696.4 0 10 102 19 696.4 0 19 103 14 696.4 0 14 104 19 696.4 0 19 105 32 696.4 0 32 106 15 696.4 0 15 107 33 696.4 0 33 108 26 696.4 0 26 109 18 696.4 0 18 110 22 696.4 0 22 111 22 696.4 0 22 112 23 696.4 0 23 113 74 696.4 0 74 114 48 696.4 0 48 115 94 696.4 0 94 116 27 696.4 0 27 117 57 696.4 0 57 118 234 696.4 0 234 119 135 696.4 0 135 120 257 696.4 0 257 121 21 696.4 0 21 122 27 696.4 0 27 123 15 696.4 0 15 124 14 696.4 0 14 125 37 696.4 0 37 126 19 696.4 0 19 127 21 696.4 0 21 128 26 696.4 0 26 129 15 696.4 0 15 130 23 696.4 0 23 131 29 696.4 0 29 132 34 696.4 0 34 133 65 696.4 0 65 134 35 696.4 0 35 135 37 696.4 0 37 136 219 696.4 0 219 137 139 696.4 0 139 138 304 696.4 0 304 139 41 696.4 0 41 140 45 696.4 0 45 141 51 696.4 0 51 142 98 696.4 0 98 143 63 696.4 0 63 144 98 696.4 0 98 145 74 696.4 0 74 146 213 696.4 0 213 147 104 696.4 0 104 148 858 696.4 0 858 149 38 696.4 0 38 150 34 696.4 0 34 151 18 696.4 0 18 152 26 696.4 0 26 153 41 696.4 0 41 154 33 696.4 0 33 155 28 696.4 0 28 156 35 696.4 0 35 157 15 696.4 0 15 158 33 696.4 0 33 159 23 696.4 0 23 160 16 696.4 0 16 161 23 696.4 0 23 162 26 696.4 0 26 163 15 696.4 0 15 164 17 696.4 0 17 165 29 696.4 0 29 166 28 696.4 0 28 167 23 696.4 0 23 168 24 696.4 0 24 169 29 696.4 0 29 170 20 696.4 0 20 171 24 696.4 0 24 172 23 696.4 0 23 173 11 696.4 0 11 174 16 696.4 0 16 175 18 696.4 0 18 176 33 696.4 0 33 177 31 696.4 0 31 178 26 696.4 0 26 179 26 696.4 0 26 180 28 696.4 0 28 181 42 696.4 0 42 182 66 696.4 0 66 183 165 696.4 0 165 184 34 696.4 0 34 185 15 696.4 0 15 186 11 696.4 0 11 187 21 696.4 0 21 188 31 696.4 0 31 189 57 696.4 0 57 190 61 696.4 0 61 191 68 696.4 0 68 192 71 696.4 0 71 193 85 696.4 0 85 194 193 696.4 0 193 195 793 696.4 0 793 196 827 696.4 0 827 197 571 696.4 0 571 198 53 696.4 0 53 199 35 696.4 0 35 200 17 696.4 0 17 201 37 696.4 0 37 202 28 696.4 0 28 203 28 696.4 0 28 204 29 696.4 0 29 205 27 696.4 0 27 206 21 696.4 0 21 207 30 696.4 0 30 208 25 696.4 0 25 209 16 696.4 0 16 210 25 696.4 0 25 211 22 696.4 0 22 212 46 696.4 0 46 213 66 696.4 0 66 214 111 696.4 0 111 215 101 696.4 0 101 216 92 696.4 0 92 217 54 696.4 0 54 218 56 696.4 0 56 219 39 696.4 0 39 220 61 696.4 0 61 221 67 696.4 0 67 222 30 696.4 0 30 223 27 696.4 0 27 224 33 696.4 0 33 225 31 696.4 0 31 226 23 696.4 0 23 227 28 696.4 0 28 228 23 696.4 0 23 229 61 696.4 0 61 230 53 696.4 0 53 231 108 696.4 0 108 232 64 696.4 0 64 233 42 696.4 0 42 234 100 696.4 0 100 235 60 696.4 0 60 236 59 696.4 0 59 237 42 696.4 0 42 238 53 696.4 0 53 239 82 696.4 0 82 240 100 696.4 0 100 241 85 696.4 0 85 242 54 696.4 0 54 243 361 696.4 0 361 244 242 696.4 0 242 245 372 696.4 0 372 246 193 696.4 0 193 247 51 696.4 0 51 248 62 696.4 0 62 249 82 696.4 0 82 250 111 696.4 0 111 251 119 696.4 0 119 252 119 696.4 0 119 253 75 696.4 0 75 254 84 696.4 0 84 255 49 696.4 0 49 256 59 696.4 0 59 257 62 696.4 0 62 258 94 696.4 0 94 259 120 696.4 0 120 260 119 696.4 0 119 261 374 696.4 0 374 262 157 696.4 0 157 263 266 696.4 0 266 264 58 696.4 0 58 265 38 696.4 0 38 266 45 696.4 0 45 267 22 696.4 0 22 268 69 696.4 0 69 269 133 696.4 0 133 270 193 696.4 0 193 271 141 696.4 0 141 272 39 696.4 0 39 273 27 696.4 0 27 274 35 696.4 0 35 275 40 696.4 0 40 276 31 696.4 0 31 277 54 696.4 0 54 278 42 696.4 0 42 279 44 696.4 0 44 280 59 696.4 0 59 281 89 696.4 0 89 282 93 696.4 0 93 283 78 696.4 0 78 284 94 696.4 0 94 285 84 696.4 0 84 286 79 696.4 0 79 287 112 696.4 0 112 288 123 696.4 0 123 289 322 696.4 0 322 290 155 696.4 0 155 291 142 696.4 0 142 292 91 696.4 0 91 293 112 696.4 0 112 294 80 696.4 0 80 295 66 696.4 0 66 296 107 696.4 0 107 297 243 696.4 0 243 298 190 696.4 0 190 299 223 696.4 0 223 300 74 696.4 0 74 301 78 696.4 0 78 Finished in 5.19 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 90,880 Reads with adapters: 38,178 (42.0%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 38,136 (42.0%) Total basepairs processed: 25,167,591 bp Total written (filtered): 10,324,749 bp (41.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38178 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 1 3 18 38 0.0 3 0 0 23 11 4 19 598 0.0 3 0 519 41 27 11 20 36642 0.0 4 34252 1903 357 95 35 21 109 0.0 4 15 79 8 6 1 22 29 0.0 4 9 4 7 3 6 23 203 0.0 4 1 1 0 159 42 24 4 0.0 4 1 0 0 1 2 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 30 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 77 1 0.0 4 1 86 16 0.0 4 0 0 0 0 16 111 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 471 0.0 4 0 0 0 0 471 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 1 235 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 237 4 0.0 4 0 0 0 0 4 238 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 270 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.42 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 38,136 Reads with adapters: 9,217 (24.2%) Reads written (passing filters): 38,136 (100.0%) Total basepairs processed: 10,324,749 bp Total written (filtered): 10,200,657 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9217 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 8.8% G: 85.5% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 595.9 0 27 4 8 149.0 0 8 6 2 9.3 0 2 7 2 2.3 0 2 8 3 0.6 0 3 9 2164 0.1 0 256 1908 10 5070 0.0 1 431 4639 11 1760 0.0 1 78 1682 12 28 0.0 1 0 28 13 15 0.0 1 0 15 14 1 0.0 1 0 1 72 1 0.0 1 0 1 139 1 0.0 1 1 149 1 0.0 1 1 150 12 0.0 1 9 3 151 3 0.0 1 2 1 152 1 0.0 1 1 207 1 0.0 1 1 208 6 0.0 1 5 1 209 1 0.0 1 1 210 2 0.0 1 2 211 1 0.0 1 0 1 258 1 0.0 1 1 269 92 0.0 1 86 6 270 12 0.0 1 12 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.