This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.36 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 91,001 (3.2%) Reads that were too short: 3,434 (0.1%) Reads written (passing filters): 87,567 (3.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,901,128 bp (2.9%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 91001 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 932 696.4 0 932 7 1014 696.4 0 1014 8 41276 696.4 0 41276 9 38396 696.4 0 38396 10 71 696.4 0 71 11 26 696.4 0 26 12 15 696.4 0 15 13 8 696.4 0 8 14 7 696.4 0 7 15 6 696.4 0 6 16 3 696.4 0 3 17 8 696.4 0 8 18 6 696.4 0 6 19 9 696.4 0 9 20 5 696.4 0 5 21 4 696.4 0 4 22 8 696.4 0 8 23 13 696.4 0 13 24 8 696.4 0 8 25 4 696.4 0 4 26 5 696.4 0 5 27 12 696.4 0 12 28 8 696.4 0 8 29 15 696.4 0 15 30 13 696.4 0 13 31 15 696.4 0 15 32 7 696.4 0 7 33 6 696.4 0 6 34 9 696.4 0 9 35 15 696.4 0 15 36 8 696.4 0 8 37 20 696.4 0 20 38 24 696.4 0 24 39 13 696.4 0 13 40 16 696.4 0 16 41 15 696.4 0 15 42 7 696.4 0 7 43 12 696.4 0 12 44 8 696.4 0 8 45 11 696.4 0 11 46 11 696.4 0 11 47 26 696.4 0 26 48 179 696.4 0 179 49 115 696.4 0 115 50 252 696.4 0 252 51 12 696.4 0 12 52 9 696.4 0 9 53 11 696.4 0 11 54 6 696.4 0 6 55 16 696.4 0 16 56 11 696.4 0 11 57 7 696.4 0 7 58 3 696.4 0 3 59 4 696.4 0 4 60 11 696.4 0 11 61 9 696.4 0 9 62 12 696.4 0 12 63 14 696.4 0 14 64 11 696.4 0 11 65 8 696.4 0 8 66 4 696.4 0 4 67 9 696.4 0 9 68 6 696.4 0 6 69 12 696.4 0 12 70 8 696.4 0 8 71 10 696.4 0 10 72 7 696.4 0 7 73 9 696.4 0 9 74 9 696.4 0 9 75 11 696.4 0 11 76 9 696.4 0 9 77 10 696.4 0 10 78 9 696.4 0 9 79 9 696.4 0 9 80 16 696.4 0 16 81 21 696.4 0 21 82 33 696.4 0 33 83 40 696.4 0 40 84 27 696.4 0 27 85 24 696.4 0 24 86 11 696.4 0 11 87 14 696.4 0 14 88 9 696.4 0 9 89 19 696.4 0 19 90 14 696.4 0 14 91 5 696.4 0 5 92 12 696.4 0 12 93 6 696.4 0 6 94 8 696.4 0 8 95 7 696.4 0 7 96 14 696.4 0 14 97 43 696.4 0 43 98 32 696.4 0 32 99 10 696.4 0 10 100 5 696.4 0 5 101 9 696.4 0 9 102 10 696.4 0 10 103 10 696.4 0 10 104 15 696.4 0 15 105 22 696.4 0 22 106 14 696.4 0 14 107 13 696.4 0 13 108 21 696.4 0 21 109 18 696.4 0 18 110 6 696.4 0 6 111 13 696.4 0 13 112 6 696.4 0 6 113 25 696.4 0 25 114 17 696.4 0 17 115 21 696.4 0 21 116 10 696.4 0 10 117 23 696.4 0 23 118 108 696.4 0 108 119 67 696.4 0 67 120 132 696.4 0 132 121 22 696.4 0 22 122 19 696.4 0 19 123 13 696.4 0 13 124 11 696.4 0 11 125 21 696.4 0 21 126 19 696.4 0 19 127 21 696.4 0 21 128 10 696.4 0 10 129 15 696.4 0 15 130 16 696.4 0 16 131 14 696.4 0 14 132 23 696.4 0 23 133 44 696.4 0 44 134 18 696.4 0 18 135 26 696.4 0 26 136 69 696.4 0 69 137 46 696.4 0 46 138 77 696.4 0 77 139 21 696.4 0 21 140 21 696.4 0 21 141 28 696.4 0 28 142 32 696.4 0 32 143 34 696.4 0 34 144 21 696.4 0 21 145 29 696.4 0 29 146 201 696.4 0 201 147 79 696.4 0 79 148 876 696.4 0 876 149 18 696.4 0 18 150 17 696.4 0 17 151 16 696.4 0 16 152 19 696.4 0 19 153 22 696.4 0 22 154 30 696.4 0 30 155 8 696.4 0 8 156 20 696.4 0 20 157 16 696.4 0 16 158 19 696.4 0 19 159 9 696.4 0 9 160 9 696.4 0 9 161 9 696.4 0 9 162 10 696.4 0 10 163 11 696.4 0 11 164 12 696.4 0 12 165 20 696.4 0 20 166 15 696.4 0 15 167 13 696.4 0 13 168 13 696.4 0 13 169 9 696.4 0 9 170 9 696.4 0 9 171 18 696.4 0 18 172 9 696.4 0 9 173 15 696.4 0 15 174 10 696.4 0 10 175 12 696.4 0 12 176 15 696.4 0 15 177 15 696.4 0 15 178 14 696.4 0 14 179 29 696.4 0 29 180 14 696.4 0 14 181 49 696.4 0 49 182 54 696.4 0 54 183 164 696.4 0 164 184 9 696.4 0 9 185 7 696.4 0 7 186 15 696.4 0 15 187 11 696.4 0 11 188 23 696.4 0 23 189 31 696.4 0 31 190 25 696.4 0 25 191 67 696.4 0 67 192 30 696.4 0 30 193 38 696.4 0 38 194 58 696.4 0 58 195 315 696.4 0 315 196 269 696.4 0 269 197 219 696.4 0 219 198 13 696.4 0 13 199 15 696.4 0 15 200 7 696.4 0 7 201 10 696.4 0 10 202 12 696.4 0 12 203 15 696.4 0 15 204 8 696.4 0 8 205 11 696.4 0 11 206 18 696.4 0 18 207 7 696.4 0 7 208 8 696.4 0 8 209 16 696.4 0 16 210 12 696.4 0 12 211 8 696.4 0 8 212 11 696.4 0 11 213 16 696.4 0 16 214 31 696.4 0 31 215 26 696.4 0 26 216 57 696.4 0 57 217 25 696.4 0 25 218 42 696.4 0 42 219 12 696.4 0 12 220 21 696.4 0 21 221 26 696.4 0 26 222 13 696.4 0 13 223 13 696.4 0 13 224 29 696.4 0 29 225 16 696.4 0 16 226 15 696.4 0 15 227 9 696.4 0 9 228 6 696.4 0 6 229 21 696.4 0 21 230 32 696.4 0 32 231 29 696.4 0 29 232 31 696.4 0 31 233 14 696.4 0 14 234 78 696.4 0 78 235 51 696.4 0 51 236 19 696.4 0 19 237 19 696.4 0 19 238 27 696.4 0 27 239 39 696.4 0 39 240 30 696.4 0 30 241 24 696.4 0 24 242 23 696.4 0 23 243 116 696.4 0 116 244 90 696.4 0 90 245 113 696.4 0 113 246 47 696.4 0 47 247 17 696.4 0 17 248 23 696.4 0 23 249 26 696.4 0 26 250 42 696.4 0 42 251 52 696.4 0 52 252 48 696.4 0 48 253 37 696.4 0 37 254 20 696.4 0 20 255 16 696.4 0 16 256 31 696.4 0 31 257 29 696.4 0 29 258 55 696.4 0 55 259 62 696.4 0 62 260 78 696.4 0 78 261 126 696.4 0 126 262 51 696.4 0 51 263 129 696.4 0 129 264 19 696.4 0 19 265 18 696.4 0 18 266 18 696.4 0 18 267 13 696.4 0 13 268 11 696.4 0 11 269 33 696.4 0 33 270 52 696.4 0 52 271 37 696.4 0 37 272 20 696.4 0 20 273 14 696.4 0 14 274 18 696.4 0 18 275 18 696.4 0 18 276 11 696.4 0 11 277 12 696.4 0 12 278 17 696.4 0 17 279 14 696.4 0 14 280 15 696.4 0 15 281 27 696.4 0 27 282 41 696.4 0 41 283 17 696.4 0 17 284 27 696.4 0 27 285 31 696.4 0 31 286 34 696.4 0 34 287 58 696.4 0 58 288 60 696.4 0 60 289 148 696.4 0 148 290 41 696.4 0 41 291 59 696.4 0 59 292 44 696.4 0 44 293 25 696.4 0 25 294 30 696.4 0 30 295 17 696.4 0 17 296 36 696.4 0 36 297 68 696.4 0 68 298 52 696.4 0 52 299 87 696.4 0 87 300 33 696.4 0 33 301 31 696.4 0 31 Finished in 4.82 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 87,567 Reads with adapters: 39,546 (45.2%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 39,503 (45.1%) Total basepairs processed: 24,901,128 bp Total written (filtered): 10,896,680 bp (43.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 39546 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 6 0.0 3 0 0 6 16 257 0.0 3 0 252 5 17 38985 0.0 3 38171 783 28 3 18 107 0.0 3 3 102 2 19 31 0.0 3 6 0 22 3 20 66 0.0 3 0 0 0 66 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 1 2 35 2 0.0 3 0 0 1 1 45 9 0.0 3 0 0 0 9 48 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 68 1 0.0 3 1 74 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 143 9 0.0 3 0 0 0 9 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 3 0.0 3 0 0 0 3 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 217 22 0.0 3 0 0 0 22 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 228 1 0.0 3 1 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.40 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 39,503 Reads with adapters: 196 (0.5%) Reads written (passing filters): 39,503 (100.0%) Total basepairs processed: 10,896,680 bp Total written (filtered): 10,896,008 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 196 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.4% C: 10.7% G: 11.2% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 124 617.2 0 124 4 68 154.3 0 68 5 1 38.6 0 1 6 2 9.6 0 2 11 1 0.0 1 0 1