This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.35 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 174,378 (6.1%) Reads that were too short: 40,355 (1.4%) Reads written (passing filters): 134,023 (4.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 35,384,455 bp (4.1%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 174378 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4843 696.4 0 4843 7 48298 696.4 0 48298 8 45208 696.4 0 45208 9 113 696.4 0 113 10 182 696.4 0 182 11 35 696.4 0 35 12 31 696.4 0 31 13 35 696.4 0 35 14 48 696.4 0 48 15 43 696.4 0 43 16 46 696.4 0 46 17 83 696.4 0 83 18 125 696.4 0 125 19 75 696.4 0 75 20 29 696.4 0 29 21 44 696.4 0 44 22 26 696.4 0 26 23 24 696.4 0 24 24 38 696.4 0 38 25 40 696.4 0 40 26 55 696.4 0 55 27 49 696.4 0 49 28 25 696.4 0 25 29 54 696.4 0 54 30 36 696.4 0 36 31 37 696.4 0 37 32 39 696.4 0 39 33 45 696.4 0 45 34 39 696.4 0 39 35 39 696.4 0 39 36 43 696.4 0 43 37 36 696.4 0 36 38 27 696.4 0 27 39 43 696.4 0 43 40 41 696.4 0 41 41 43 696.4 0 43 42 52 696.4 0 52 43 45 696.4 0 45 44 33 696.4 0 33 45 26 696.4 0 26 46 62 696.4 0 62 47 75 696.4 0 75 48 79 696.4 0 79 49 116 696.4 0 116 50 51 696.4 0 51 51 48 696.4 0 48 52 69 696.4 0 69 53 102 696.4 0 102 54 67 696.4 0 67 55 60 696.4 0 60 56 45 696.4 0 45 57 65 696.4 0 65 58 54 696.4 0 54 59 54 696.4 0 54 60 108 696.4 0 108 61 66 696.4 0 66 62 75 696.4 0 75 63 68 696.4 0 68 64 80 696.4 0 80 65 55 696.4 0 55 66 67 696.4 0 67 67 91 696.4 0 91 68 58 696.4 0 58 69 52 696.4 0 52 70 129 696.4 0 129 71 118 696.4 0 118 72 163 696.4 0 163 73 624 696.4 0 624 74 690 696.4 0 690 75 901 696.4 0 901 76 378 696.4 0 378 77 3361 696.4 0 3361 78 1889 696.4 0 1889 79 1694 696.4 0 1694 80 36 696.4 0 36 81 40 696.4 0 40 82 56 696.4 0 56 83 51 696.4 0 51 84 51 696.4 0 51 85 48 696.4 0 48 86 105 696.4 0 105 87 42 696.4 0 42 88 75 696.4 0 75 89 60 696.4 0 60 90 70 696.4 0 70 91 55 696.4 0 55 92 45 696.4 0 45 93 47 696.4 0 47 94 42 696.4 0 42 95 38 696.4 0 38 96 44 696.4 0 44 97 52 696.4 0 52 98 34 696.4 0 34 99 59 696.4 0 59 100 42 696.4 0 42 101 56 696.4 0 56 102 32 696.4 0 32 103 56 696.4 0 56 104 45 696.4 0 45 105 64 696.4 0 64 106 266 696.4 0 266 107 507 696.4 0 507 108 145 696.4 0 145 109 41 696.4 0 41 110 56 696.4 0 56 111 37 696.4 0 37 112 81 696.4 0 81 113 69 696.4 0 69 114 117 696.4 0 117 115 73 696.4 0 73 116 75 696.4 0 75 117 56 696.4 0 56 118 40 696.4 0 40 119 48 696.4 0 48 120 48 696.4 0 48 121 47 696.4 0 47 122 45 696.4 0 45 123 44 696.4 0 44 124 59 696.4 0 59 125 68 696.4 0 68 126 74 696.4 0 74 127 118 696.4 0 118 128 197 696.4 0 197 129 206 696.4 0 206 130 232 696.4 0 232 131 110 696.4 0 110 132 150 696.4 0 150 133 176 696.4 0 176 134 183 696.4 0 183 135 119 696.4 0 119 136 143 696.4 0 143 137 67 696.4 0 67 138 90 696.4 0 90 139 93 696.4 0 93 140 148 696.4 0 148 141 89 696.4 0 89 142 69 696.4 0 69 143 139 696.4 0 139 144 147 696.4 0 147 145 235 696.4 0 235 146 3940 696.4 0 3940 147 2195 696.4 0 2195 148 2778 696.4 0 2778 149 88 696.4 0 88 150 203 696.4 0 203 151 53 696.4 0 53 152 47 696.4 0 47 153 63 696.4 0 63 154 71 696.4 0 71 155 196 696.4 0 196 156 183 696.4 0 183 157 138 696.4 0 138 158 77 696.4 0 77 159 100 696.4 0 100 160 101 696.4 0 101 161 102 696.4 0 102 162 56 696.4 0 56 163 58 696.4 0 58 164 227 696.4 0 227 165 222 696.4 0 222 166 351 696.4 0 351 167 171 696.4 0 171 168 91 696.4 0 91 169 61 696.4 0 61 170 59 696.4 0 59 171 52 696.4 0 52 172 53 696.4 0 53 173 56 696.4 0 56 174 66 696.4 0 66 175 41 696.4 0 41 176 43 696.4 0 43 177 59 696.4 0 59 178 71 696.4 0 71 179 75 696.4 0 75 180 78 696.4 0 78 181 97 696.4 0 97 182 69 696.4 0 69 183 70 696.4 0 70 184 243 696.4 0 243 185 285 696.4 0 285 186 361 696.4 0 361 187 117 696.4 0 117 188 74 696.4 0 74 189 53 696.4 0 53 190 56 696.4 0 56 191 61 696.4 0 61 192 84 696.4 0 84 193 81 696.4 0 81 194 100 696.4 0 100 195 631 696.4 0 631 196 451 696.4 0 451 197 556 696.4 0 556 198 252 696.4 0 252 199 133 696.4 0 133 200 100 696.4 0 100 201 85 696.4 0 85 202 61 696.4 0 61 203 51 696.4 0 51 204 91 696.4 0 91 205 106 696.4 0 106 206 92 696.4 0 92 207 66 696.4 0 66 208 68 696.4 0 68 209 103 696.4 0 103 210 70 696.4 0 70 211 80 696.4 0 80 212 80 696.4 0 80 213 84 696.4 0 84 214 89 696.4 0 89 215 209 696.4 0 209 216 137 696.4 0 137 217 145 696.4 0 145 218 87 696.4 0 87 219 162 696.4 0 162 220 80 696.4 0 80 221 98 696.4 0 98 222 77 696.4 0 77 223 65 696.4 0 65 224 90 696.4 0 90 225 79 696.4 0 79 226 97 696.4 0 97 227 96 696.4 0 96 228 83 696.4 0 83 229 81 696.4 0 81 230 87 696.4 0 87 231 128 696.4 0 128 232 151 696.4 0 151 233 229 696.4 0 229 234 376 696.4 0 376 235 761 696.4 0 761 236 6962 696.4 0 6962 237 7962 696.4 0 7962 238 4391 696.4 0 4391 239 161 696.4 0 161 240 92 696.4 0 92 241 134 696.4 0 134 242 82 696.4 0 82 243 73 696.4 0 73 244 94 696.4 0 94 245 142 696.4 0 142 246 220 696.4 0 220 247 336 696.4 0 336 248 499 696.4 0 499 249 294 696.4 0 294 250 156 696.4 0 156 251 254 696.4 0 254 252 382 696.4 0 382 253 408 696.4 0 408 254 293 696.4 0 293 255 319 696.4 0 319 256 166 696.4 0 166 257 91 696.4 0 91 258 70 696.4 0 70 259 85 696.4 0 85 260 66 696.4 0 66 261 58 696.4 0 58 262 68 696.4 0 68 263 88 696.4 0 88 264 84 696.4 0 84 265 101 696.4 0 101 266 99 696.4 0 99 267 80 696.4 0 80 268 98 696.4 0 98 269 87 696.4 0 87 270 118 696.4 0 118 271 100 696.4 0 100 272 99 696.4 0 99 273 119 696.4 0 119 274 111 696.4 0 111 275 97 696.4 0 97 276 135 696.4 0 135 277 187 696.4 0 187 278 277 696.4 0 277 279 200 696.4 0 200 280 382 696.4 0 382 281 518 696.4 0 518 282 647 696.4 0 647 283 694 696.4 0 694 284 610 696.4 0 610 285 434 696.4 0 434 286 412 696.4 0 412 287 275 696.4 0 275 288 166 696.4 0 166 289 153 696.4 0 153 290 102 696.4 0 102 291 128 696.4 0 128 292 165 696.4 0 165 293 286 696.4 0 286 294 305 696.4 0 305 295 490 696.4 0 490 296 1249 696.4 0 1249 297 1318 696.4 0 1318 298 741 696.4 0 741 299 376 696.4 0 376 300 239 696.4 0 239 301 368 696.4 0 368 Finished in 7.19 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 134,023 Reads with adapters: 48,248 (36.0%) Reads that were too short: 75 (0.1%) Reads written (passing filters): 48,173 (35.9%) Total basepairs processed: 35,384,455 bp Total written (filtered): 13,132,210 bp (37.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 48248 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 31 0.0 3 0 0 18 7 6 19 718 0.0 3 0 594 60 47 17 20 45716 0.0 4 42925 2280 387 93 31 21 151 0.0 4 36 104 8 2 1 22 1207 0.0 4 1091 81 25 7 3 23 142 0.0 4 0 1 1 94 46 24 4 0.0 4 1 0 0 1 2 25 5 0.0 4 0 0 0 2 3 26 3 0.0 4 0 0 0 0 3 27 2 0.0 4 0 0 0 1 1 32 1 0.0 4 0 0 0 0 1 34 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 2 0.0 4 1 0 0 0 1 57 37 0.0 4 0 0 0 0 37 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 20 0.0 4 0 0 0 0 20 75 1 0.0 4 0 0 0 0 1 83 5 0.0 4 0 0 0 0 5 85 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 107 0.0 4 0 0 0 0 107 132 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 3 0.0 4 0 0 0 0 3 163 2 0.0 4 0 0 0 0 2 164 2 0.0 4 0 0 0 1 1 166 3 0.0 4 0 0 0 0 3 167 16 0.0 4 0 0 0 0 16 168 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 2 8 229 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 236 3 0.0 4 0 0 0 1 2 237 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 1 257 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 1 283 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 48,173 Reads with adapters: 7,549 (15.7%) Reads written (passing filters): 48,173 (100.0%) Total basepairs processed: 13,132,210 bp Total written (filtered): 13,047,639 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7549 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 9.9% G: 84.4% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 95 752.7 0 95 4 18 188.2 0 18 6 7 11.8 0 7 8 1 0.7 0 1 9 55 0.2 0 1 54 10 1908 0.0 1 161 1747 11 5269 0.0 1 269 5000 12 155 0.0 1 6 149 13 12 0.0 1 0 12 14 3 0.0 1 0 3 15 2 0.0 1 0 2 44 1 0.0 1 1 151 4 0.0 1 3 1 152 1 0.0 1 1 190 1 0.0 1 0 1 208 1 0.0 1 1 209 16 0.0 1 13 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.