This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.10 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 162,725 (5.7%) Reads that were too short: 33,470 (1.2%) Reads written (passing filters): 129,255 (4.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 34,979,129 bp (4.1%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 162725 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4013 696.4 0 4013 7 47359 696.4 0 47359 8 50798 696.4 0 50798 9 88 696.4 0 88 10 127 696.4 0 127 11 28 696.4 0 28 12 33 696.4 0 33 13 38 696.4 0 38 14 47 696.4 0 47 15 36 696.4 0 36 16 36 696.4 0 36 17 33 696.4 0 33 18 38 696.4 0 38 19 36 696.4 0 36 20 34 696.4 0 34 21 42 696.4 0 42 22 33 696.4 0 33 23 23 696.4 0 23 24 39 696.4 0 39 25 37 696.4 0 37 26 41 696.4 0 41 27 33 696.4 0 33 28 46 696.4 0 46 29 50 696.4 0 50 30 47 696.4 0 47 31 35 696.4 0 35 32 34 696.4 0 34 33 44 696.4 0 44 34 32 696.4 0 32 35 43 696.4 0 43 36 41 696.4 0 41 37 37 696.4 0 37 38 35 696.4 0 35 39 39 696.4 0 39 40 54 696.4 0 54 41 32 696.4 0 32 42 49 696.4 0 49 43 47 696.4 0 47 44 37 696.4 0 37 45 47 696.4 0 47 46 74 696.4 0 74 47 53 696.4 0 53 48 43 696.4 0 43 49 54 696.4 0 54 50 37 696.4 0 37 51 36 696.4 0 36 52 70 696.4 0 70 53 70 696.4 0 70 54 55 696.4 0 55 55 55 696.4 0 55 56 53 696.4 0 53 57 54 696.4 0 54 58 47 696.4 0 47 59 48 696.4 0 48 60 83 696.4 0 83 61 77 696.4 0 77 62 1867 696.4 0 1867 63 96 696.4 0 96 64 61 696.4 0 61 65 52 696.4 0 52 66 45 696.4 0 45 67 62 696.4 0 62 68 52 696.4 0 52 69 47 696.4 0 47 70 54 696.4 0 54 71 64 696.4 0 64 72 81 696.4 0 81 73 386 696.4 0 386 74 486 696.4 0 486 75 544 696.4 0 544 76 119 696.4 0 119 77 1302 696.4 0 1302 78 733 696.4 0 733 79 708 696.4 0 708 80 56 696.4 0 56 81 47 696.4 0 47 82 65 696.4 0 65 83 37 696.4 0 37 84 29 696.4 0 29 85 53 696.4 0 53 86 35 696.4 0 35 87 38 696.4 0 38 88 57 696.4 0 57 89 63 696.4 0 63 90 43 696.4 0 43 91 45 696.4 0 45 92 57 696.4 0 57 93 42 696.4 0 42 94 41 696.4 0 41 95 42 696.4 0 42 96 50 696.4 0 50 97 55 696.4 0 55 98 41 696.4 0 41 99 52 696.4 0 52 100 39 696.4 0 39 101 52 696.4 0 52 102 41 696.4 0 41 103 57 696.4 0 57 104 67 696.4 0 67 105 75 696.4 0 75 106 330 696.4 0 330 107 663 696.4 0 663 108 142 696.4 0 142 109 59 696.4 0 59 110 45 696.4 0 45 111 47 696.4 0 47 112 69 696.4 0 69 113 53 696.4 0 53 114 108 696.4 0 108 115 60 696.4 0 60 116 44 696.4 0 44 117 60 696.4 0 60 118 45 696.4 0 45 119 49 696.4 0 49 120 63 696.4 0 63 121 60 696.4 0 60 122 54 696.4 0 54 123 59 696.4 0 59 124 52 696.4 0 52 125 61 696.4 0 61 126 72 696.4 0 72 127 111 696.4 0 111 128 142 696.4 0 142 129 154 696.4 0 154 130 205 696.4 0 205 131 93 696.4 0 93 132 158 696.4 0 158 133 129 696.4 0 129 134 143 696.4 0 143 135 103 696.4 0 103 136 119 696.4 0 119 137 58 696.4 0 58 138 69 696.4 0 69 139 108 696.4 0 108 140 111 696.4 0 111 141 59 696.4 0 59 142 61 696.4 0 61 143 113 696.4 0 113 144 102 696.4 0 102 145 190 696.4 0 190 146 3334 696.4 0 3334 147 1817 696.4 0 1817 148 2357 696.4 0 2357 149 90 696.4 0 90 150 223 696.4 0 223 151 51 696.4 0 51 152 31 696.4 0 31 153 43 696.4 0 43 154 62 696.4 0 62 155 167 696.4 0 167 156 174 696.4 0 174 157 105 696.4 0 105 158 45 696.4 0 45 159 60 696.4 0 60 160 53 696.4 0 53 161 78 696.4 0 78 162 47 696.4 0 47 163 46 696.4 0 46 164 117 696.4 0 117 165 120 696.4 0 120 166 169 696.4 0 169 167 94 696.4 0 94 168 32 696.4 0 32 169 46 696.4 0 46 170 41 696.4 0 41 171 42 696.4 0 42 172 39 696.4 0 39 173 49 696.4 0 49 174 50 696.4 0 50 175 36 696.4 0 36 176 27 696.4 0 27 177 34 696.4 0 34 178 53 696.4 0 53 179 57 696.4 0 57 180 35 696.4 0 35 181 46 696.4 0 46 182 49 696.4 0 49 183 38 696.4 0 38 184 118 696.4 0 118 185 160 696.4 0 160 186 153 696.4 0 153 187 83 696.4 0 83 188 49 696.4 0 49 189 43 696.4 0 43 190 27 696.4 0 27 191 51 696.4 0 51 192 52 696.4 0 52 193 51 696.4 0 51 194 71 696.4 0 71 195 445 696.4 0 445 196 295 696.4 0 295 197 286 696.4 0 286 198 114 696.4 0 114 199 88 696.4 0 88 200 49 696.4 0 49 201 52 696.4 0 52 202 46 696.4 0 46 203 50 696.4 0 50 204 68 696.4 0 68 205 80 696.4 0 80 206 70 696.4 0 70 207 34 696.4 0 34 208 33 696.4 0 33 209 85 696.4 0 85 210 39 696.4 0 39 211 32 696.4 0 32 212 47 696.4 0 47 213 46 696.4 0 46 214 50 696.4 0 50 215 129 696.4 0 129 216 111 696.4 0 111 217 99 696.4 0 99 218 66 696.4 0 66 219 106 696.4 0 106 220 80 696.4 0 80 221 57 696.4 0 57 222 42 696.4 0 42 223 53 696.4 0 53 224 60 696.4 0 60 225 46 696.4 0 46 226 49 696.4 0 49 227 37 696.4 0 37 228 52 696.4 0 52 229 55 696.4 0 55 230 71 696.4 0 71 231 120 696.4 0 120 232 122 696.4 0 122 233 214 696.4 0 214 234 321 696.4 0 321 235 569 696.4 0 569 236 7869 696.4 0 7869 237 9892 696.4 0 9892 238 5232 696.4 0 5232 239 79 696.4 0 79 240 44 696.4 0 44 241 56 696.4 0 56 242 38 696.4 0 38 243 33 696.4 0 33 244 35 696.4 0 35 245 65 696.4 0 65 246 88 696.4 0 88 247 142 696.4 0 142 248 176 696.4 0 176 249 90 696.4 0 90 250 56 696.4 0 56 251 78 696.4 0 78 252 105 696.4 0 105 253 107 696.4 0 107 254 72 696.4 0 72 255 82 696.4 0 82 256 54 696.4 0 54 257 36 696.4 0 36 258 44 696.4 0 44 259 40 696.4 0 40 260 29 696.4 0 29 261 40 696.4 0 40 262 30 696.4 0 30 263 47 696.4 0 47 264 44 696.4 0 44 265 54 696.4 0 54 266 45 696.4 0 45 267 48 696.4 0 48 268 54 696.4 0 54 269 56 696.4 0 56 270 36 696.4 0 36 271 68 696.4 0 68 272 47 696.4 0 47 273 52 696.4 0 52 274 43 696.4 0 43 275 41 696.4 0 41 276 73 696.4 0 73 277 103 696.4 0 103 278 164 696.4 0 164 279 98 696.4 0 98 280 186 696.4 0 186 281 372 696.4 0 372 282 401 696.4 0 401 283 452 696.4 0 452 284 228 696.4 0 228 285 176 696.4 0 176 286 135 696.4 0 135 287 74 696.4 0 74 288 66 696.4 0 66 289 83 696.4 0 83 290 77 696.4 0 77 291 65 696.4 0 65 292 83 696.4 0 83 293 161 696.4 0 161 294 134 696.4 0 134 295 231 696.4 0 231 296 457 696.4 0 457 297 462 696.4 0 462 298 276 696.4 0 276 299 164 696.4 0 164 300 116 696.4 0 116 301 247 696.4 0 247 Finished in 6.35 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 129,255 Reads with adapters: 48,448 (37.5%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 48,405 (37.4%) Total basepairs processed: 34,979,129 bp Total written (filtered): 13,382,898 bp (38.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 48448 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 317 0.0 3 0 310 7 17 47704 0.0 3 46724 949 29 2 18 117 0.0 3 12 99 6 19 15 0.0 3 1 0 10 4 20 70 0.0 3 0 0 0 70 22 1 0.0 3 0 0 0 1 23 1 0.0 3 1 28 1 0.0 3 1 35 1 0.0 3 1 39 23 0.0 3 0 0 0 23 40 1 0.0 3 0 0 0 1 45 49 0.0 3 0 0 0 49 48 5 0.0 3 0 0 0 5 56 1 0.0 3 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 6 0.0 3 0 0 0 6 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 49 0.0 3 0 0 0 49 115 15 0.0 3 0 0 0 15 117 4 0.0 3 0 0 0 4 118 4 0.0 3 0 0 0 4 119 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 1 143 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 174 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 216 3 0.0 3 0 0 0 3 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.77 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 48,405 Reads with adapters: 97 (0.2%) Reads written (passing filters): 48,405 (100.0%) Total basepairs processed: 13,382,898 bp Total written (filtered): 13,382,550 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 97 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.6% C: 30.9% G: 36.1% T: 13.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 77 756.3 0 77 4 7 189.1 0 7 5 4 47.3 0 4 6 6 11.8 0 6 9 1 0.2 0 0 1 12 2 0.0 1 0 2