This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.35 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 77,054 (2.7%) Reads that were too short: 48,702 (1.7%) Reads written (passing filters): 28,352 (1.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 5,977,307 bp (0.7%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 77054 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 170 696.4 0 170 7 4989 696.4 0 4989 8 85 696.4 0 85 9 3892 696.4 0 3892 10 21 696.4 0 21 11 10 696.4 0 10 12 25 696.4 0 25 13 16 696.4 0 16 14 11 696.4 0 11 15 5 696.4 0 5 16 11 696.4 0 11 17 16 696.4 0 16 18 15 696.4 0 15 19 11 696.4 0 11 20 9 696.4 0 9 21 9 696.4 0 9 22 12 696.4 0 12 23 11 696.4 0 11 24 23 696.4 0 23 25 13 696.4 0 13 26 9 696.4 0 9 27 6 696.4 0 6 28 5 696.4 0 5 29 8 696.4 0 8 30 10 696.4 0 10 31 10 696.4 0 10 32 19 696.4 0 19 33 15 696.4 0 15 34 10 696.4 0 10 35 16 696.4 0 16 36 24 696.4 0 24 37 13 696.4 0 13 38 15 696.4 0 15 39 17 696.4 0 17 40 9 696.4 0 9 41 22 696.4 0 22 42 15 696.4 0 15 43 96 696.4 0 96 44 13 696.4 0 13 45 6 696.4 0 6 46 12 696.4 0 12 47 8 696.4 0 8 48 16 696.4 0 16 49 10 696.4 0 10 50 10 696.4 0 10 51 12 696.4 0 12 52 7 696.4 0 7 53 12 696.4 0 12 54 18 696.4 0 18 55 1219 696.4 0 1219 56 52 696.4 0 52 57 13 696.4 0 13 58 12 696.4 0 12 59 15 696.4 0 15 60 9 696.4 0 9 61 17 696.4 0 17 62 11 696.4 0 11 63 8 696.4 0 8 64 11 696.4 0 11 65 15 696.4 0 15 66 20 696.4 0 20 67 19 696.4 0 19 68 18 696.4 0 18 69 23 696.4 0 23 70 44 696.4 0 44 71 47 696.4 0 47 72 44 696.4 0 44 73 20 696.4 0 20 74 28 696.4 0 28 75 52 696.4 0 52 76 103 696.4 0 103 77 79 696.4 0 79 78 94 696.4 0 94 79 50 696.4 0 50 80 135 696.4 0 135 81 379 696.4 0 379 82 223 696.4 0 223 83 131 696.4 0 131 84 36 696.4 0 36 85 37 696.4 0 37 86 32 696.4 0 32 87 35 696.4 0 35 88 19 696.4 0 19 89 36 696.4 0 36 90 36 696.4 0 36 91 22 696.4 0 22 92 30 696.4 0 30 93 27 696.4 0 27 94 34 696.4 0 34 95 393 696.4 0 393 96 58 696.4 0 58 97 30 696.4 0 30 98 61 696.4 0 61 99 31 696.4 0 31 100 44 696.4 0 44 101 229 696.4 0 229 102 294 696.4 0 294 103 345 696.4 0 345 104 151 696.4 0 151 105 69 696.4 0 69 106 72 696.4 0 72 107 100 696.4 0 100 108 102 696.4 0 102 109 131 696.4 0 131 110 157 696.4 0 157 111 171 696.4 0 171 112 144 696.4 0 144 113 137 696.4 0 137 114 103 696.4 0 103 115 118 696.4 0 118 116 97 696.4 0 97 117 102 696.4 0 102 118 147 696.4 0 147 119 240 696.4 0 240 120 98 696.4 0 98 121 62 696.4 0 62 122 118 696.4 0 118 123 141 696.4 0 141 124 173 696.4 0 173 125 139 696.4 0 139 126 116 696.4 0 116 127 133 696.4 0 133 128 70 696.4 0 70 129 90 696.4 0 90 130 101 696.4 0 101 131 139 696.4 0 139 132 244 696.4 0 244 133 553 696.4 0 553 134 250 696.4 0 250 135 300 696.4 0 300 136 110 696.4 0 110 137 113 696.4 0 113 138 202 696.4 0 202 139 209 696.4 0 209 140 218 696.4 0 218 141 150 696.4 0 150 142 94 696.4 0 94 143 102 696.4 0 102 144 163 696.4 0 163 145 150 696.4 0 150 146 300 696.4 0 300 147 221 696.4 0 221 148 255 696.4 0 255 149 119 696.4 0 119 150 76 696.4 0 76 151 121 696.4 0 121 152 107 696.4 0 107 153 159 696.4 0 159 154 158 696.4 0 158 155 127 696.4 0 127 156 180 696.4 0 180 157 183 696.4 0 183 158 238 696.4 0 238 159 140 696.4 0 140 160 151 696.4 0 151 161 142 696.4 0 142 162 110 696.4 0 110 163 96 696.4 0 96 164 119 696.4 0 119 165 147 696.4 0 147 166 130 696.4 0 130 167 106 696.4 0 106 168 94 696.4 0 94 169 89 696.4 0 89 170 111 696.4 0 111 171 121 696.4 0 121 172 155 696.4 0 155 173 134 696.4 0 134 174 106 696.4 0 106 175 80 696.4 0 80 176 81 696.4 0 81 177 86 696.4 0 86 178 85 696.4 0 85 179 120 696.4 0 120 180 259 696.4 0 259 181 256 696.4 0 256 182 218 696.4 0 218 183 90 696.4 0 90 184 124 696.4 0 124 185 162 696.4 0 162 186 101 696.4 0 101 187 102 696.4 0 102 188 116 696.4 0 116 189 96 696.4 0 96 190 134 696.4 0 134 191 108 696.4 0 108 192 98 696.4 0 98 193 105 696.4 0 105 194 134 696.4 0 134 195 94 696.4 0 94 196 93 696.4 0 93 197 99 696.4 0 99 198 130 696.4 0 130 199 142 696.4 0 142 200 130 696.4 0 130 201 118 696.4 0 118 202 80 696.4 0 80 203 142 696.4 0 142 204 124 696.4 0 124 205 165 696.4 0 165 206 245 696.4 0 245 207 234 696.4 0 234 208 303 696.4 0 303 209 443 696.4 0 443 210 1250 696.4 0 1250 211 10456 696.4 0 10456 212 11624 696.4 0 11624 213 4691 696.4 0 4691 214 1399 696.4 0 1399 215 121 696.4 0 121 216 96 696.4 0 96 217 91 696.4 0 91 218 162 696.4 0 162 219 153 696.4 0 153 220 126 696.4 0 126 221 97 696.4 0 97 222 85 696.4 0 85 223 138 696.4 0 138 224 234 696.4 0 234 225 176 696.4 0 176 226 190 696.4 0 190 227 121 696.4 0 121 228 131 696.4 0 131 229 126 696.4 0 126 230 111 696.4 0 111 231 98 696.4 0 98 232 90 696.4 0 90 233 91 696.4 0 91 234 129 696.4 0 129 235 103 696.4 0 103 236 102 696.4 0 102 237 132 696.4 0 132 238 132 696.4 0 132 239 133 696.4 0 133 240 137 696.4 0 137 241 118 696.4 0 118 242 99 696.4 0 99 243 120 696.4 0 120 244 275 696.4 0 275 245 216 696.4 0 216 246 737 696.4 0 737 247 109 696.4 0 109 248 70 696.4 0 70 249 80 696.4 0 80 250 96 696.4 0 96 251 104 696.4 0 104 252 124 696.4 0 124 253 109 696.4 0 109 254 118 696.4 0 118 255 146 696.4 0 146 256 196 696.4 0 196 257 173 696.4 0 173 258 185 696.4 0 185 259 135 696.4 0 135 260 218 696.4 0 218 261 135 696.4 0 135 262 119 696.4 0 119 263 90 696.4 0 90 264 97 696.4 0 97 265 132 696.4 0 132 266 192 696.4 0 192 267 271 696.4 0 271 268 205 696.4 0 205 269 120 696.4 0 120 270 292 696.4 0 292 271 249 696.4 0 249 272 519 696.4 0 519 273 237 696.4 0 237 274 390 696.4 0 390 275 270 696.4 0 270 276 196 696.4 0 196 277 253 696.4 0 253 278 187 696.4 0 187 279 262 696.4 0 262 280 179 696.4 0 179 281 135 696.4 0 135 282 129 696.4 0 129 283 120 696.4 0 120 284 99 696.4 0 99 285 119 696.4 0 119 286 137 696.4 0 137 287 205 696.4 0 205 288 194 696.4 0 194 289 156 696.4 0 156 290 138 696.4 0 138 291 201 696.4 0 201 292 864 696.4 0 864 293 924 696.4 0 924 294 1020 696.4 0 1020 295 493 696.4 0 493 296 182 696.4 0 182 297 108 696.4 0 108 298 152 696.4 0 152 299 161 696.4 0 161 300 256 696.4 0 256 301 625 696.4 0 625 Finished in 1.38 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 28,352 Reads with adapters: 5,073 (17.9%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 5,050 (17.8%) Total basepairs processed: 5,977,307 bp Total written (filtered): 1,382,720 bp (23.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5073 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 45 0.0 3 0 38 6 1 20 4967 0.0 4 4720 196 33 12 6 21 19 0.0 4 5 12 1 1 23 8 0.0 4 0 1 0 3 4 24 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 5,050 Reads with adapters: 264 (5.2%) Reads written (passing filters): 5,050 (100.0%) Total basepairs processed: 1,382,720 bp Total written (filtered): 1,379,641 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 264 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.4% C: 9.1% G: 83.3% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 78.9 0 11 9 1 0.0 0 0 1 10 1 0.0 1 0 1 11 47 0.0 1 1 46 12 203 0.0 1 1 202 74 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.