This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.47 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 77,205 (2.7%) Reads that were too short: 48,153 (1.7%) Reads written (passing filters): 29,052 (1.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 6,134,734 bp (0.7%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 77205 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 121 696.4 0 121 7 4790 696.4 0 4790 8 62 696.4 0 62 9 4439 696.4 0 4439 10 14 696.4 0 14 11 21 696.4 0 21 12 21 696.4 0 21 13 14 696.4 0 14 14 12 696.4 0 12 15 20 696.4 0 20 16 14 696.4 0 14 17 24 696.4 0 24 18 15 696.4 0 15 19 14 696.4 0 14 20 17 696.4 0 17 21 19 696.4 0 19 22 13 696.4 0 13 23 18 696.4 0 18 24 19 696.4 0 19 25 10 696.4 0 10 26 16 696.4 0 16 27 17 696.4 0 17 28 9 696.4 0 9 29 12 696.4 0 12 30 20 696.4 0 20 31 6 696.4 0 6 32 20 696.4 0 20 33 21 696.4 0 21 34 101 696.4 0 101 35 19 696.4 0 19 36 21 696.4 0 21 37 17 696.4 0 17 38 18 696.4 0 18 39 24 696.4 0 24 40 12 696.4 0 12 41 33 696.4 0 33 42 803 696.4 0 803 43 169 696.4 0 169 44 13 696.4 0 13 45 13 696.4 0 13 46 16 696.4 0 16 47 17 696.4 0 17 48 16 696.4 0 16 49 10 696.4 0 10 50 11 696.4 0 11 51 12 696.4 0 12 52 16 696.4 0 16 53 10 696.4 0 10 54 15 696.4 0 15 55 18 696.4 0 18 56 17 696.4 0 17 57 15 696.4 0 15 58 12 696.4 0 12 59 18 696.4 0 18 60 15 696.4 0 15 61 15 696.4 0 15 62 19 696.4 0 19 63 14 696.4 0 14 64 22 696.4 0 22 65 25 696.4 0 25 66 21 696.4 0 21 67 23 696.4 0 23 68 5 696.4 0 5 69 25 696.4 0 25 70 24 696.4 0 24 71 25 696.4 0 25 72 24 696.4 0 24 73 12 696.4 0 12 74 17 696.4 0 17 75 21 696.4 0 21 76 16 696.4 0 16 77 24 696.4 0 24 78 72 696.4 0 72 79 71 696.4 0 71 80 93 696.4 0 93 81 471 696.4 0 471 82 289 696.4 0 289 83 258 696.4 0 258 84 104 696.4 0 104 85 158 696.4 0 158 86 153 696.4 0 153 87 129 696.4 0 129 88 90 696.4 0 90 89 74 696.4 0 74 90 77 696.4 0 77 91 73 696.4 0 73 92 78 696.4 0 78 93 77 696.4 0 77 94 69 696.4 0 69 95 114 696.4 0 114 96 68 696.4 0 68 97 80 696.4 0 80 98 84 696.4 0 84 99 43 696.4 0 43 100 45 696.4 0 45 101 104 696.4 0 104 102 199 696.4 0 199 103 224 696.4 0 224 104 154 696.4 0 154 105 84 696.4 0 84 106 66 696.4 0 66 107 114 696.4 0 114 108 84 696.4 0 84 109 119 696.4 0 119 110 156 696.4 0 156 111 149 696.4 0 149 112 140 696.4 0 140 113 124 696.4 0 124 114 115 696.4 0 115 115 125 696.4 0 125 116 78 696.4 0 78 117 128 696.4 0 128 118 129 696.4 0 129 119 229 696.4 0 229 120 127 696.4 0 127 121 77 696.4 0 77 122 100 696.4 0 100 123 117 696.4 0 117 124 187 696.4 0 187 125 177 696.4 0 177 126 128 696.4 0 128 127 157 696.4 0 157 128 93 696.4 0 93 129 108 696.4 0 108 130 89 696.4 0 89 131 128 696.4 0 128 132 200 696.4 0 200 133 396 696.4 0 396 134 258 696.4 0 258 135 313 696.4 0 313 136 118 696.4 0 118 137 121 696.4 0 121 138 219 696.4 0 219 139 223 696.4 0 223 140 227 696.4 0 227 141 153 696.4 0 153 142 91 696.4 0 91 143 104 696.4 0 104 144 143 696.4 0 143 145 142 696.4 0 142 146 341 696.4 0 341 147 232 696.4 0 232 148 266 696.4 0 266 149 136 696.4 0 136 150 86 696.4 0 86 151 159 696.4 0 159 152 140 696.4 0 140 153 159 696.4 0 159 154 134 696.4 0 134 155 143 696.4 0 143 156 209 696.4 0 209 157 160 696.4 0 160 158 246 696.4 0 246 159 139 696.4 0 139 160 150 696.4 0 150 161 124 696.4 0 124 162 119 696.4 0 119 163 116 696.4 0 116 164 163 696.4 0 163 165 151 696.4 0 151 166 149 696.4 0 149 167 107 696.4 0 107 168 114 696.4 0 114 169 112 696.4 0 112 170 126 696.4 0 126 171 119 696.4 0 119 172 149 696.4 0 149 173 132 696.4 0 132 174 124 696.4 0 124 175 90 696.4 0 90 176 83 696.4 0 83 177 102 696.4 0 102 178 78 696.4 0 78 179 149 696.4 0 149 180 236 696.4 0 236 181 185 696.4 0 185 182 166 696.4 0 166 183 111 696.4 0 111 184 140 696.4 0 140 185 164 696.4 0 164 186 144 696.4 0 144 187 122 696.4 0 122 188 101 696.4 0 101 189 109 696.4 0 109 190 128 696.4 0 128 191 130 696.4 0 130 192 131 696.4 0 131 193 105 696.4 0 105 194 144 696.4 0 144 195 88 696.4 0 88 196 106 696.4 0 106 197 80 696.4 0 80 198 139 696.4 0 139 199 174 696.4 0 174 200 140 696.4 0 140 201 124 696.4 0 124 202 94 696.4 0 94 203 141 696.4 0 141 204 127 696.4 0 127 205 190 696.4 0 190 206 226 696.4 0 226 207 206 696.4 0 206 208 352 696.4 0 352 209 439 696.4 0 439 210 905 696.4 0 905 211 10777 696.4 0 10777 212 12389 696.4 0 12389 213 5175 696.4 0 5175 214 1579 696.4 0 1579 215 169 696.4 0 169 216 83 696.4 0 83 217 106 696.4 0 106 218 136 696.4 0 136 219 171 696.4 0 171 220 161 696.4 0 161 221 111 696.4 0 111 222 80 696.4 0 80 223 127 696.4 0 127 224 258 696.4 0 258 225 173 696.4 0 173 226 193 696.4 0 193 227 111 696.4 0 111 228 114 696.4 0 114 229 136 696.4 0 136 230 123 696.4 0 123 231 115 696.4 0 115 232 111 696.4 0 111 233 105 696.4 0 105 234 140 696.4 0 140 235 145 696.4 0 145 236 150 696.4 0 150 237 124 696.4 0 124 238 130 696.4 0 130 239 144 696.4 0 144 240 131 696.4 0 131 241 124 696.4 0 124 242 124 696.4 0 124 243 142 696.4 0 142 244 295 696.4 0 295 245 202 696.4 0 202 246 714 696.4 0 714 247 146 696.4 0 146 248 92 696.4 0 92 249 84 696.4 0 84 250 81 696.4 0 81 251 107 696.4 0 107 252 110 696.4 0 110 253 107 696.4 0 107 254 117 696.4 0 117 255 103 696.4 0 103 256 165 696.4 0 165 257 139 696.4 0 139 258 139 696.4 0 139 259 129 696.4 0 129 260 164 696.4 0 164 261 116 696.4 0 116 262 141 696.4 0 141 263 116 696.4 0 116 264 123 696.4 0 123 265 114 696.4 0 114 266 154 696.4 0 154 267 177 696.4 0 177 268 164 696.4 0 164 269 103 696.4 0 103 270 211 696.4 0 211 271 185 696.4 0 185 272 345 696.4 0 345 273 213 696.4 0 213 274 261 696.4 0 261 275 233 696.4 0 233 276 152 696.4 0 152 277 208 696.4 0 208 278 164 696.4 0 164 279 209 696.4 0 209 280 137 696.4 0 137 281 113 696.4 0 113 282 128 696.4 0 128 283 96 696.4 0 96 284 130 696.4 0 130 285 139 696.4 0 139 286 128 696.4 0 128 287 249 696.4 0 249 288 158 696.4 0 158 289 143 696.4 0 143 290 115 696.4 0 115 291 156 696.4 0 156 292 681 696.4 0 681 293 601 696.4 0 601 294 679 696.4 0 679 295 341 696.4 0 341 296 121 696.4 0 121 297 120 696.4 0 120 298 119 696.4 0 119 299 165 696.4 0 165 300 213 696.4 0 213 301 601 696.4 0 601 Finished in 1.20 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 29,052 Reads with adapters: 4,563 (15.7%) Reads that were too short: 15 (0.1%) Reads written (passing filters): 4,548 (15.7%) Total basepairs processed: 6,134,734 bp Total written (filtered): 1,248,193 bp (20.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4563 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 31 0.0 3 0 31 17 4472 0.0 3 4394 74 3 1 18 11 0.0 3 0 11 19 6 0.0 3 0 0 5 1 20 5 0.0 3 0 0 0 5 21 2 0.0 3 1 0 0 1 27 1 0.0 3 0 0 0 1 33 2 0.0 3 1 0 0 1 36 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 6 0.0 3 0 0 0 6 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 4,548 Reads with adapters: 130 (2.9%) Reads written (passing filters): 4,548 (100.0%) Total basepairs processed: 1,248,193 bp Total written (filtered): 1,247,802 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 130 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 88.5% C: 7.7% G: 3.1% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 129 71.1 0 129 4 1 17.8 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.